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The map label for this gene is buk [H]

Identifier: 52786322

GI number: 52786322

Start: 2502574

End: 2503680

Strand: Reverse

Name: buk [H]

Synonym: BLi02584

Alternate gene names: 52786322

Gene position: 2503680-2502574 (Counterclockwise)

Preceding gene: 52786323

Following gene: 52786321

Centisome position: 59.29

GC content: 49.41

Gene sequence:

>1107_bases
ATGCAGGTACAGGAAAAACGTATTCTCGTCATCAATCCGGGATCTACATCTACAAAGATCGGCGTTTTTCATGATGACCG
TTCGATTTTCGAAAAATCAATCCGTCATGACGAGGCTGAGCTACAGCAATATCAGACCATTATTGATCAATATTCGTTCA
GAAAACAGGCGATACTCGAAACCCTGCATGAACAGGGAATCAATATTTCTAAATTGGATGCCGTTTGCGCCAGGGGAGGG
CTGCTTCGGCCGATTGAAGGCGGCACTTACGAAGTCAATGATGCGATGATTGTCGATTTGAAAAACGGCTATGCGGGGCA
GCATGCATCAAATCTCGGGGGCATCATCGCCAGGGAGATTGCCGACGGGTTAAATATTCCCGCTTTTATCGTCGACCCCG
TTGTTGTGGATGAAATGGCTCCTATCGCAAAAATTTCCGGCACCCCGGCTATTGAAAGGCGCAGCATTTTTCACGCGCTC
AACCAAAAAGCAGTTGCAAGGAAAGCGGCTTGGCAGTTTGGGAAGCGTTATGAAGATATGAAAATGATCATCACCCACAT
GGGAGGCGGCATTACGATCGGCGTCCATTGCCGCGGCCGGGTGATCGACGTCAACAACGGCCTCCACGGGGAAGGTCCGC
TCAGTCCAGAGCGGGCCGGAACCATTCCTGCGGGTGATCTGATCGATATGTGCTTTTCCGGCGAATATACGAAAGACGAG
CTGATGAAAATGCTTGTCGGCGGCGGAGGGCTTGCCGGCTATCTCGGCACGACGGATGCGGTAAAAGTTGAGAAAATGAT
CAAGGAAGGCGATCAAAAAGCTGCGCTCATCTATGAAGCGATGGCTTATCAAATCGCCAAAGAAATCGGGGCGGCCAGCG
CCGTCTTAAAAGGCGAAGTCGATGTCATTATTTTGACAGGAGGACTGGCATATGGAAAATCGTTTATTTCCTCGATCAGA
CAATACATAGACTGGATTTCGGATGTCGTCGTCTTTCCAGGAGAAAATGAACTTCAAGCATTGGCTGAAGGTGCATTTCG
CGTATTGAACGGCGAAGAAGAGGCAAAACAGTATCCGAACCAGAGGAGGGAAAGTCATGGCAACTGA

Upstream 100 bases:

>100_bases
GCATGCGCCAATCGAGAAGCCAATTTTTGCAAAACGGCCATCACATTTTAAGCAGACGTTAAAATCATAACCCCTATTCT
CTATTGGAGGTTTTAATTCA

Downstream 100 bases:

>100_bases
ATACGATCTCGTGATTCTTGGAGGGGGCACAGGCGGCTATGTGGCCGCCATCAGAGCCTCGCAGCTGGGGCTGAAAACAG
CCGTCGTTGAAAAGCAGAAG

Product: butyrate kinase

Products: NA

Alternate protein names: BK; Branched-chain carboxylic acid kinase [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MQVQEKRILVINPGSTSTKIGVFHDDRSIFEKSIRHDEAELQQYQTIIDQYSFRKQAILETLHEQGINISKLDAVCARGG
LLRPIEGGTYEVNDAMIVDLKNGYAGQHASNLGGIIAREIADGLNIPAFIVDPVVVDEMAPIAKISGTPAIERRSIFHAL
NQKAVARKAAWQFGKRYEDMKMIITHMGGGITIGVHCRGRVIDVNNGLHGEGPLSPERAGTIPAGDLIDMCFSGEYTKDE
LMKMLVGGGGLAGYLGTTDAVKVEKMIKEGDQKAALIYEAMAYQIAKEIGAASAVLKGEVDVIILTGGLAYGKSFISSIR
QYIDWISDVVVFPGENELQALAEGAFRVLNGEEEAKQYPNQRRESHGN

Sequences:

>Translated_368_residues
MQVQEKRILVINPGSTSTKIGVFHDDRSIFEKSIRHDEAELQQYQTIIDQYSFRKQAILETLHEQGINISKLDAVCARGG
LLRPIEGGTYEVNDAMIVDLKNGYAGQHASNLGGIIAREIADGLNIPAFIVDPVVVDEMAPIAKISGTPAIERRSIFHAL
NQKAVARKAAWQFGKRYEDMKMIITHMGGGITIGVHCRGRVIDVNNGLHGEGPLSPERAGTIPAGDLIDMCFSGEYTKDE
LMKMLVGGGGLAGYLGTTDAVKVEKMIKEGDQKAALIYEAMAYQIAKEIGAASAVLKGEVDVIILTGGLAYGKSFISSIR
QYIDWISDVVVFPGENELQALAEGAFRVLNGEEEAKQYPNQRRESHGN
>Mature_368_residues
MQVQEKRILVINPGSTSTKIGVFHDDRSIFEKSIRHDEAELQQYQTIIDQYSFRKQAILETLHEQGINISKLDAVCARGG
LLRPIEGGTYEVNDAMIVDLKNGYAGQHASNLGGIIAREIADGLNIPAFIVDPVVVDEMAPIAKISGTPAIERRSIFHAL
NQKAVARKAAWQFGKRYEDMKMIITHMGGGITIGVHCRGRVIDVNNGLHGEGPLSPERAGTIPAGDLIDMCFSGEYTKDE
LMKMLVGGGGLAGYLGTTDAVKVEKMIKEGDQKAALIYEAMAYQIAKEIGAASAVLKGEVDVIILTGGLAYGKSFISSIR
QYIDWISDVVVFPGENELQALAEGAFRVLNGEEEAKQYPNQRRESHGN

Specific function: Anaerobic degradation of L-threonine to propionate. [C]

COG id: COG3426

COG function: function code C; Butyrate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI145693184, Length=216, Percent_Identity=26.8518518518519, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR011245 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.7 [H]

Molecular weight: Translated: 40087; Mature: 40087

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVQEKRILVINPGSTSTKIGVFHDDRSIFEKSIRHDEAELQQYQTIIDQYSFRKQAILE
CCCCCCEEEEECCCCCCCEEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
TLHEQGINISKLDAVCARGGLLRPIEGGTYEVNDAMIVDLKNGYAGQHASNLGGIIAREI
HHHHCCCCHHHHHHHHHCCCCEECCCCCEEEECCEEEEEECCCCCCCCHHHHHHHHHHHH
ADGLNIPAFIVDPVVVDEMAPIAKISGTPAIERRSIFHALNQKAVARKAAWQFGKRYEDM
HCCCCCCHHEECCHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMIITHMGGGITIGVHCRGRVIDVNNGLHGEGPLSPERAGTIPAGDLIDMCFSGEYTKDE
HHHHHHCCCCEEEEEEECEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHH
LMKMLVGGGGLAGYLGTTDAVKVEKMIKEGDQKAALIYEAMAYQIAKEIGAASAVLKGEV
HHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DVIILTGGLAYGKSFISSIRQYIDWISDVVVFPGENELQALAEGAFRVLNGEEEAKQYPN
EEEEEECCHHHHHHHHHHHHHHHHHHHCEEECCCCHHHHHHHHHHHHHCCCHHHHHHCCH
QRRESHGN
HHHHCCCC
>Mature Secondary Structure
MQVQEKRILVINPGSTSTKIGVFHDDRSIFEKSIRHDEAELQQYQTIIDQYSFRKQAILE
CCCCCCEEEEECCCCCCCEEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
TLHEQGINISKLDAVCARGGLLRPIEGGTYEVNDAMIVDLKNGYAGQHASNLGGIIAREI
HHHHCCCCHHHHHHHHHCCCCEECCCCCEEEECCEEEEEECCCCCCCCHHHHHHHHHHHH
ADGLNIPAFIVDPVVVDEMAPIAKISGTPAIERRSIFHALNQKAVARKAAWQFGKRYEDM
HCCCCCCHHEECCHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMIITHMGGGITIGVHCRGRVIDVNNGLHGEGPLSPERAGTIPAGDLIDMCFSGEYTKDE
HHHHHHCCCCEEEEEEECEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHH
LMKMLVGGGGLAGYLGTTDAVKVEKMIKEGDQKAALIYEAMAYQIAKEIGAASAVLKGEV
HHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DVIILTGGLAYGKSFISSIRQYIDWISDVVVFPGENELQALAEGAFRVLNGEEEAKQYPN
EEEEEECCHHHHHHHHHHHHHHHHHHHCEEECCCCHHHHHHHHHHHHHCCCHHHHHHCCH
QRRESHGN
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]