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The map label for this gene is lpdV [H]

Identifier: 52786321

GI number: 52786321

Start: 2501163

End: 2502584

Strand: Reverse

Name: lpdV [H]

Synonym: BLi02583

Alternate gene names: 52786321

Gene position: 2502584-2501163 (Counterclockwise)

Preceding gene: 52786322

Following gene: 52786320

Centisome position: 59.27

GC content: 52.04

Gene sequence:

>1422_bases
ATGGCAACTGAATACGATCTCGTGATTCTTGGAGGGGGCACAGGCGGCTATGTGGCCGCCATCAGAGCCTCGCAGCTGGG
GCTGAAAACAGCCGTCGTTGAAAAGCAGAAGCTCGGCGGCACGTGCCTTCATAAAGGCTGCATCCCGTCGAAGGCGCTCC
TTAGAAGCGCGGAAGTATACAGGACAGCAAAAAAGGCGGACGAATTCGGAGTGGTCATTCCTGAAGTCGGACTGCGGTTT
TCTCAAGTTCAAAGCAGAAAACAAAAAATAATTGACCAGCTGCACAACGGTGTCAAACACTTGATGAAAAAAGGAAAGAT
CGATGTTTACGAAGGAATCGGACGGATTCTCGGGCCGTCGATTTTTTCCCCGATGCCTGGGACGGTTTCCGTTGAGATGG
CGAACGGGGATGAAAATGAGATGCTCATACCGAAAAACGTCATCATCGCTACAGGCTCAAGGCCCAGGTCGCTGCCAGGG
CTTGAGCTTGACGGAGAAAACGTTCTGTCATCAGACGAAGCGCTTGAACTTGAGCAGCTTCCGGCTTCCATGCTGATCGT
CGGAGGAGGTGTCATCGGGATCGAGTGGGCTTCCATGCTGAACGATTTCGGTGTTGATGTCACCGTCATTGAGTATGCAG
ACCGGATTTTGCCGACTGAAGATGCCGATATTTCTAGGGAAATGCAGGCGCAGCTCGCGAAAAAAGGCATCACAATGATC
ACGGGAGCAAAAGTGCTGCCCGACACATTGGAGAAAGGCGATGCGGTCAGCATTCAAGCGGAAAAAGACGGAGAAAAACA
ATCCTATTCCGCTGAGAAAATGCTCGTATCGGTCGGAAGGCAGGCAAATATTGAGGGTATCGGTCTGGAAAACACGGATA
TCCAGGTGGAAAACGGCTTTATCGTGACAAACAGCATGTACCAGACAAAAGAATCCCACATCTATGCGATAGGAGATGTG
ATCGGCGGCCTTCAGCTTGCACATGTAGCTTCTCACGAAGGGATTACGGCCGTTGAGCATATTGCTGGTGAAAACCCGCA
CGCCATCGATTATTCGCTTGTTTCAAAATGCATCTATTCAAGCCCTGAAGCCGCATCGGTCGGCTTGACAGAGGGTGAGG
CGAAGGCGCAGGGGAAAAGCGTTAAAGTAGGCAAATTTCCGTTTCAGGCGATTGGCAAAGCGCTCGTGTACGGTGAGACG
GACGGCTTTGTCAAAATCGTCGCCGATCGCGAAACAGATGACATTTTGGGCGTTCACATGATCGGCCCGCATGTGACAGA
CATGATATCTGAAGCCGGTCTGGCAAAGGTGCTTGACGCCACCCCGTGGGAAGTCGGTCAAACGATCCACCCGCATCCGA
CGCTGTCGGAGGCGATCGGAGAAGCCGCCCTTGCCGTGGATGGAAAAGCGATTCATTTTTAG

Upstream 100 bases:

>100_bases
CCAGGAGAAAATGAACTTCAAGCATTGGCTGAAGGTGCATTTCGCGTATTGAACGGCGAAGAAGAGGCAAAACAGTATCC
GAACCAGAGGAGGGAAAGTC

Downstream 100 bases:

>100_bases
AAAGCATGAAGGAGTGAAAAAAATGGGCAATAACCGTCATGAAGCAGTGGGATTAACAGATGAACAAGCAATCGACATGT
ACAAAACCATGCTTTTAGCC

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of branched-chain alpha-keto acid dehydrogenase complex; LPD-Val [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MATEYDLVILGGGTGGYVAAIRASQLGLKTAVVEKQKLGGTCLHKGCIPSKALLRSAEVYRTAKKADEFGVVIPEVGLRF
SQVQSRKQKIIDQLHNGVKHLMKKGKIDVYEGIGRILGPSIFSPMPGTVSVEMANGDENEMLIPKNVIIATGSRPRSLPG
LELDGENVLSSDEALELEQLPASMLIVGGGVIGIEWASMLNDFGVDVTVIEYADRILPTEDADISREMQAQLAKKGITMI
TGAKVLPDTLEKGDAVSIQAEKDGEKQSYSAEKMLVSVGRQANIEGIGLENTDIQVENGFIVTNSMYQTKESHIYAIGDV
IGGLQLAHVASHEGITAVEHIAGENPHAIDYSLVSKCIYSSPEAASVGLTEGEAKAQGKSVKVGKFPFQAIGKALVYGET
DGFVKIVADRETDDILGVHMIGPHVTDMISEAGLAKVLDATPWEVGQTIHPHPTLSEAIGEAALAVDGKAIHF

Sequences:

>Translated_473_residues
MATEYDLVILGGGTGGYVAAIRASQLGLKTAVVEKQKLGGTCLHKGCIPSKALLRSAEVYRTAKKADEFGVVIPEVGLRF
SQVQSRKQKIIDQLHNGVKHLMKKGKIDVYEGIGRILGPSIFSPMPGTVSVEMANGDENEMLIPKNVIIATGSRPRSLPG
LELDGENVLSSDEALELEQLPASMLIVGGGVIGIEWASMLNDFGVDVTVIEYADRILPTEDADISREMQAQLAKKGITMI
TGAKVLPDTLEKGDAVSIQAEKDGEKQSYSAEKMLVSVGRQANIEGIGLENTDIQVENGFIVTNSMYQTKESHIYAIGDV
IGGLQLAHVASHEGITAVEHIAGENPHAIDYSLVSKCIYSSPEAASVGLTEGEAKAQGKSVKVGKFPFQAIGKALVYGET
DGFVKIVADRETDDILGVHMIGPHVTDMISEAGLAKVLDATPWEVGQTIHPHPTLSEAIGEAALAVDGKAIHF
>Mature_472_residues
ATEYDLVILGGGTGGYVAAIRASQLGLKTAVVEKQKLGGTCLHKGCIPSKALLRSAEVYRTAKKADEFGVVIPEVGLRFS
QVQSRKQKIIDQLHNGVKHLMKKGKIDVYEGIGRILGPSIFSPMPGTVSVEMANGDENEMLIPKNVIIATGSRPRSLPGL
ELDGENVLSSDEALELEQLPASMLIVGGGVIGIEWASMLNDFGVDVTVIEYADRILPTEDADISREMQAQLAKKGITMIT
GAKVLPDTLEKGDAVSIQAEKDGEKQSYSAEKMLVSVGRQANIEGIGLENTDIQVENGFIVTNSMYQTKESHIYAIGDVI
GGLQLAHVASHEGITAVEHIAGENPHAIDYSLVSKCIYSSPEAASVGLTEGEAKAQGKSVKVGKFPFQAIGKALVYGETD
GFVKIVADRETDDILGVHMIGPHVTDMISEAGLAKVLDATPWEVGQTIHPHPTLSEAIGEAALAVDGKAIHF

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=477, Percent_Identity=40.251572327044, Blast_Score=317, Evalue=1e-86,
Organism=Homo sapiens, GI50301238, Length=466, Percent_Identity=27.0386266094421, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI22035672, Length=480, Percent_Identity=27.7083333333333, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI33519430, Length=462, Percent_Identity=24.4588744588745, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI33519428, Length=462, Percent_Identity=24.4588744588745, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI33519426, Length=462, Percent_Identity=24.4588744588745, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI148277065, Length=462, Percent_Identity=24.4588744588745, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI148277071, Length=462, Percent_Identity=24.4588744588745, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI291045266, Length=483, Percent_Identity=24.8447204968944, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI291045268, Length=480, Percent_Identity=23.9583333333333, Blast_Score=96, Evalue=1e-19,
Organism=Escherichia coli, GI1786307, Length=473, Percent_Identity=36.1522198731501, Blast_Score=279, Evalue=3e-76,
Organism=Escherichia coli, GI87082354, Length=478, Percent_Identity=29.2887029288703, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI87081717, Length=468, Percent_Identity=28.2051282051282, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI1789915, Length=465, Percent_Identity=27.741935483871, Blast_Score=154, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI32565766, Length=473, Percent_Identity=41.8604651162791, Blast_Score=340, Evalue=7e-94,
Organism=Caenorhabditis elegans, GI17557007, Length=491, Percent_Identity=24.847250509165, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71982272, Length=496, Percent_Identity=26.008064516129, Blast_Score=117, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI71983419, Length=460, Percent_Identity=25.8695652173913, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71983429, Length=460, Percent_Identity=26.0869565217391, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321091, Length=482, Percent_Identity=39.4190871369295, Blast_Score=300, Evalue=4e-82,
Organism=Saccharomyces cerevisiae, GI6325240, Length=481, Percent_Identity=29.7297297297297, Blast_Score=190, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6325166, Length=471, Percent_Identity=26.5392781316348, Blast_Score=158, Evalue=2e-39,
Organism=Drosophila melanogaster, GI21358499, Length=478, Percent_Identity=41.8410041841004, Blast_Score=331, Evalue=8e-91,
Organism=Drosophila melanogaster, GI24640553, Length=493, Percent_Identity=25.5578093306288, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24640549, Length=493, Percent_Identity=25.5578093306288, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24640551, Length=493, Percent_Identity=25.5578093306288, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI17737741, Length=479, Percent_Identity=25.4697286012526, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50285; Mature: 50154

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATEYDLVILGGGTGGYVAAIRASQLGLKTAVVEKQKLGGTCLHKGCIPSKALLRSAEVY
CCCCEEEEEEECCCCCEEEEEEHHHCCHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHH
RTAKKADEFGVVIPEVGLRFSQVQSRKQKIIDQLHNGVKHLMKKGKIDVYEGIGRILGPS
HHHHHHHHCCEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHH
IFSPMPGTVSVEMANGDENEMLIPKNVIIATGSRPRSLPGLELDGENVLSSDEALELEQL
HCCCCCCEEEEEECCCCCCCEECCCCEEEEECCCCCCCCCCEECCCCCCCCCCHHHHHHC
PASMLIVGGGVIGIEWASMLNDFGVDVTVIEYADRILPTEDADISREMQAQLAKKGITMI
CCEEEEECCCEEEHHHHHHHHHCCCCEEEEEHHHHHCCCCCCCHHHHHHHHHHHCCCEEE
TGAKVLPDTLEKGDAVSIQAEKDGEKQSYSAEKMLVSVGRQANIEGIGLENTDIQVENGF
ECCCCCHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCE
IVTNSMYQTKESHIYAIGDVIGGLQLAHVASHEGITAVEHIAGENPHAIDYSLVSKCIYS
EEECCHHHCCCCCEEEEHHHHCCHHHHHHHCCCCCHHHHHHCCCCCCEECHHHHHHHHCC
SPEAASVGLTEGEAKAQGKSVKVGKFPFQAIGKALVYGETDGFVKIVADRETDDILGVHM
CCCCCCCCCCCCCHHCCCCEEEECCCCHHHHCCEEEEECCCCEEEEEECCCCCCEEEEEE
IGPHVTDMISEAGLAKVLDATPWEVGQTIHPHPTLSEAIGEAALAVDGKAIHF
CCCHHHHHHHHCCHHHHHCCCCHHHCCCCCCCCCHHHHHCCCEEEECCCEECC
>Mature Secondary Structure 
ATEYDLVILGGGTGGYVAAIRASQLGLKTAVVEKQKLGGTCLHKGCIPSKALLRSAEVY
CCCEEEEEEECCCCCEEEEEEHHHCCHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHH
RTAKKADEFGVVIPEVGLRFSQVQSRKQKIIDQLHNGVKHLMKKGKIDVYEGIGRILGPS
HHHHHHHHCCEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHH
IFSPMPGTVSVEMANGDENEMLIPKNVIIATGSRPRSLPGLELDGENVLSSDEALELEQL
HCCCCCCEEEEEECCCCCCCEECCCCEEEEECCCCCCCCCCEECCCCCCCCCCHHHHHHC
PASMLIVGGGVIGIEWASMLNDFGVDVTVIEYADRILPTEDADISREMQAQLAKKGITMI
CCEEEEECCCEEEHHHHHHHHHCCCCEEEEEHHHHHCCCCCCCHHHHHHHHHHHCCCEEE
TGAKVLPDTLEKGDAVSIQAEKDGEKQSYSAEKMLVSVGRQANIEGIGLENTDIQVENGF
ECCCCCHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCE
IVTNSMYQTKESHIYAIGDVIGGLQLAHVASHEGITAVEHIAGENPHAIDYSLVSKCIYS
EEECCHHHCCCCCEEEEHHHHCCHHHHHHHCCCCCHHHHHHCCCCCCEECHHHHHHHHCC
SPEAASVGLTEGEAKAQGKSVKVGKFPFQAIGKALVYGETDGFVKIVADRETDDILGVHM
CCCCCCCCCCCCCHHCCCCEEEECCCCHHHHCCEEEEECCCCEEEEEECCCCCCEEEEEE
IGPHVTDMISEAGLAKVLDATPWEVGQTIHPHPTLSEAIGEAALAVDGKAIHF
CCCHHHHHHHHCCHHHHHCCCCHHHCCCCCCCCCHHHHHCCCEEEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 8504804 [H]