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The map label for this gene is bcd

Identifier: 52786323

GI number: 52786323

Start: 2503719

End: 2504813

Strand: Reverse

Name: bcd

Synonym: BLi02585

Alternate gene names: 52786323

Gene position: 2504813-2503719 (Counterclockwise)

Preceding gene: 52786324

Following gene: 52786322

Centisome position: 59.32

GC content: 51.69

Gene sequence:

>1095_bases
ATGGAACTATTTCGATATATGGAACAGTATGACTACGAGCAATTGGTATTTTGCCAAGATAAACAGTCCGGTTTAAAAGC
GATCATCGCGATTCATGATACGACGCTCGGGCCGGCTCTCGGCGGCACAAGAATGTGGACATACGAAAGTGAAGAAGCCG
CAATTGAAGATGCGCTGCGCCTTGCGCGGGGAATGACCTACAAAAATGCGGCGGCCGGACTGAACCTCGGAGGAGGCAAA
ACCGTTATTATCGGAGATCCGCGCAAAGATAAAAACGAAGAAATGTTCCGCGCTTTCGGCCGCTACATTCAAGGCTTGAA
CGGCAGATACATCACAGCCGAAGACGTCGGTACAACCGTTGAAGATATGGACATCATTCATGACGAAACCGATTTCGTTA
CAGGCATTTCACCTGCTTTCGGTTCATCAGGAAATCCTTCTCCGGTAACAGCTTACGGGGTATATAAAGGGATGAAGGCG
GCGGCGAAAGCGGCATTCGGAACGGATTCGCTTGAAGGCAAAACCGTTGCGGTTCAAGGCGTCGGAAACGTGGCCTACAA
CCTGTGCCGGCACCTCCACGAAGAAGGCGCGAAACTGATCGTGACCGACATCAACAAAGAAGCAGTTGAACGTGCAGTCG
CCGAATTCGGCGCCCGCGCCGTCGATCCGGATGATATTTATTCGCAGGAATGCGATATATATGCGCCGTGTGCCCTCGGA
GCGACAATCAACGATGATACGATTCCGCAGCTGAAAGCCAAAGTGATTGCCGGGGCAGCCAACAACCAGCTGAAAGAAAC
CCGCCACGGTGATCAAATCCACGACATGGGCATCGTTTATGCCCCGGACTATGTCATCAATGCCGGCGGCGTCATCAATG
TCGCTGACGAGCTTTACGGCTATAATTCGGAGCGCGCGCTGAAGAAAGTCGAAGGCATCTACGGAAACATTGAACGCGTC
CTTGAAATTTCGAAGCGCGACCGCATTCCGACATACTTGGCCGCAGACCGTCTGGCGGAAGAACGAATTGAGCGCATGCG
CCAATCGAGAAGCCAATTTTTGCAAAACGGCCATCACATTTTAAGCAGACGTTAA

Upstream 100 bases:

>100_bases
CATCGCCTTGACAAGCAGGGCCGATTCCGCAGAAAACAAGTTGTATTCGATTGCTTTGGCGCTGTGTACGTCTGAAGCAC
GACATGAGGAGGAATAAAAA

Downstream 100 bases:

>100_bases
AATCATAACCCCTATTCTCTATTGGAGGTTTTAATTCAATGCAGGTACAGGAAAAACGTATTCTCGTCATCAATCCGGGA
TCTACATCTACAAAGATCGG

Product: leucine dehydrogenase

Products: NA

Alternate protein names: LeuDH

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MELFRYMEQYDYEQLVFCQDKQSGLKAIIAIHDTTLGPALGGTRMWTYESEEAAIEDALRLARGMTYKNAAAGLNLGGGK
TVIIGDPRKDKNEEMFRAFGRYIQGLNGRYITAEDVGTTVEDMDIIHDETDFVTGISPAFGSSGNPSPVTAYGVYKGMKA
AAKAAFGTDSLEGKTVAVQGVGNVAYNLCRHLHEEGAKLIVTDINKEAVERAVAEFGARAVDPDDIYSQECDIYAPCALG
ATINDDTIPQLKAKVIAGAANNQLKETRHGDQIHDMGIVYAPDYVINAGGVINVADELYGYNSERALKKVEGIYGNIERV
LEISKRDRIPTYLAADRLAEERIERMRQSRSQFLQNGHHILSRR

Sequences:

>Translated_364_residues
MELFRYMEQYDYEQLVFCQDKQSGLKAIIAIHDTTLGPALGGTRMWTYESEEAAIEDALRLARGMTYKNAAAGLNLGGGK
TVIIGDPRKDKNEEMFRAFGRYIQGLNGRYITAEDVGTTVEDMDIIHDETDFVTGISPAFGSSGNPSPVTAYGVYKGMKA
AAKAAFGTDSLEGKTVAVQGVGNVAYNLCRHLHEEGAKLIVTDINKEAVERAVAEFGARAVDPDDIYSQECDIYAPCALG
ATINDDTIPQLKAKVIAGAANNQLKETRHGDQIHDMGIVYAPDYVINAGGVINVADELYGYNSERALKKVEGIYGNIERV
LEISKRDRIPTYLAADRLAEERIERMRQSRSQFLQNGHHILSRR
>Mature_364_residues
MELFRYMEQYDYEQLVFCQDKQSGLKAIIAIHDTTLGPALGGTRMWTYESEEAAIEDALRLARGMTYKNAAAGLNLGGGK
TVIIGDPRKDKNEEMFRAFGRYIQGLNGRYITAEDVGTTVEDMDIIHDETDFVTGISPAFGSSGNPSPVTAYGVYKGMKA
AAKAAFGTDSLEGKTVAVQGVGNVAYNLCRHLHEEGAKLIVTDINKEAVERAVAEFGARAVDPDDIYSQECDIYAPCALG
ATINDDTIPQLKAKVIAGAANNQLKETRHGDQIHDMGIVYAPDYVINAGGVINVADELYGYNSERALKKVEGIYGNIERV
LEISKRDRIPTYLAADRLAEERIERMRQSRSQFLQNGHHILSRR

Specific function: Functions catabolically in the bacterial metabolism of branched-chain L-amino acids, and plays an important role in spore germination in cooperation with alanine dehydrogenase

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family

Homologues:

Organism=Homo sapiens, GI31377775, Length=305, Percent_Identity=27.2131147540984, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI4885281, Length=305, Percent_Identity=27.2131147540984, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17544676, Length=311, Percent_Identity=26.3665594855305, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24649283, Length=297, Percent_Identity=28.956228956229, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI45549226, Length=306, Percent_Identity=27.4509803921569, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI45553475, Length=306, Percent_Identity=27.4509803921569, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24649460, Length=271, Percent_Identity=27.6752767527675, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): DHLE_BACLI (Q53560)

Other databases:

- EMBL:   S81735
- ProteinModelPortal:   Q53560
- SMR:   Q53560
- BRENDA:   1.4.1.9
- GO:   GO:0005488
- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR016211
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000188
- PRINTS:   PR00082
- SMART:   SM00839

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N

EC number: =1.4.1.9

Molecular weight: Translated: 40041; Mature: 40041

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: ACT_SITE 80-80

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELFRYMEQYDYEQLVFCQDKQSGLKAIIAIHDTTLGPALGGTRMWTYESEEAAIEDALR
CHHHHHHHHCCCCCEEEECCCCCCCEEEEEEECCCCCCCCCCCEEEEECCHHHHHHHHHH
LARGMTYKNAAAGLNLGGGKTVIIGDPRKDKNEEMFRAFGRYIQGLNGRYITAEDVGTTV
HHHCCCCCHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEHHHCCCCH
EDMDIIHDETDFVTGISPAFGSSGNPSPVTAYGVYKGMKAAAKAAFGTDSLEGKTVAVQG
HHHHHHCCCCHHHCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCEEEEEC
VGNVAYNLCRHLHEEGAKLIVTDINKEAVERAVAEFGARAVDPDDIYSQECDIYAPCALG
CHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCCCCHHHHHCCCCCEEECEECC
ATINDDTIPQLKAKVIAGAANNQLKETRHGDQIHDMGIVYAPDYVINAGGVINVADELYG
CCCCCCCHHHHHHHHHHCCCCCHHHHHHCCCCHHHCCEEECCCEEEECCCEEEEHHHHCC
YNSERALKKVEGIYGNIERVLEISKRDRIPTYLAADRLAEERIERMRQSRSQFLQNGHHI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LSRR
HCCC
>Mature Secondary Structure
MELFRYMEQYDYEQLVFCQDKQSGLKAIIAIHDTTLGPALGGTRMWTYESEEAAIEDALR
CHHHHHHHHCCCCCEEEECCCCCCCEEEEEEECCCCCCCCCCCEEEEECCHHHHHHHHHH
LARGMTYKNAAAGLNLGGGKTVIIGDPRKDKNEEMFRAFGRYIQGLNGRYITAEDVGTTV
HHHCCCCCHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEHHHCCCCH
EDMDIIHDETDFVTGISPAFGSSGNPSPVTAYGVYKGMKAAAKAAFGTDSLEGKTVAVQG
HHHHHHCCCCHHHCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCEEEEEC
VGNVAYNLCRHLHEEGAKLIVTDINKEAVERAVAEFGARAVDPDDIYSQECDIYAPCALG
CHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCCCCHHHHHCCCCCEEECEECC
ATINDDTIPQLKAKVIAGAANNQLKETRHGDQIHDMGIVYAPDYVINAGGVINVADELYG
CCCCCCCHHHHHHHHHHCCCCCHHHHHHCCCCHHHCCEEECCCEEEECCCEEEEHHHHCC
YNSERALKKVEGIYGNIERVLEISKRDRIPTYLAADRLAEERIERMRQSRSQFLQNGHHI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LSRR
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8597545