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The map label for this gene is bkdAA [H]

Identifier: 52786320

GI number: 52786320

Start: 2500148

End: 2501140

Strand: Reverse

Name: bkdAA [H]

Synonym: BLi02582

Alternate gene names: 52786320

Gene position: 2501140-2500148 (Counterclockwise)

Preceding gene: 52786321

Following gene: 52786319

Centisome position: 59.23

GC content: 49.55

Gene sequence:

>993_bases
ATGGGCAATAACCGTCATGAAGCAGTGGGATTAACAGATGAACAAGCAATCGACATGTACAAAACCATGCTTTTAGCCAG
AAAACTCGATGAGCGGATGTGGCTCTTAAACCGTTCAGGCAAAATTCCTTTTGTCATCTCTTGTCAAGGACAGGAAGCAG
CCCAGGTCGGAGCGGCTTTCGCTTTAGACCGGGAGAACGATTATGTGCTTCCGTATTACAGAGATATGGGCGTTGTGCTT
GCTTTTGGAATGACGGCAAAAGATTTAATGCTGTCGGCGTTTGCAAAAGCCGAGGATCCGAATTCAGGAGGAAAGCAGAT
GCCAAGCCACTTCGGCCAAAAGAAAAACCGGATCGTCACGGGCTCTTCGCCGGTGACCACGCAAGTTCCGCATGCGGTGG
GCGTAGCGCTTGCCGGAAGGCTTGACAAGAAAGACATTGCAACTTTTGTGACGTTCGGCGAAGGCTCTTCAAACCAAGGG
GACTTCCATGAAGGGGCAAACTTTGCCGCCGTGCATAAGCTTCCCGTCATTTTTATGTGTGAAAACAATAAATACGCGAT
CTCGGTTCCTTATGAAAAGCAGGTAGCTTGCGAAAACATTTCCGACCGCGCCGTCGGTTACGGCATGCCTGGCGTGACCG
TTGACGGAAATGATCCGCTTGAAGTCTACAAAGCTGTTAAAGAAGCAAGGGAACGGGCCGGAAGAGGCGAAGGGCCGACA
TTAATCGAAACGATATCATACCGGCTGACCCCGCACTCCAGCGATGATGACGACCGAAGCTACAGAGAGCGCGAAGAGGT
GGCGGAGGCGAAGAAAAATGATCCGCTTAACAAATTCGCACAATACTTAAAAGAAGCAGGTCTTCTGGACGACCAGTCTG
AGAAGCAAATATCGGATGAGATCACAAAAATCGTCAATGAAGCAACAGATTATGCTGAACATGCACCTTATGCAGATCCG
GAAGATGCGCTGCGGCATGTTTATGCGGAGTAA

Upstream 100 bases:

>100_bases
ATCCACCCGCATCCGACGCTGTCGGAGGCGATCGGAGAAGCCGCCCTTGCCGTGGATGGAAAAGCGATTCATTTTTAGAA
AGCATGAAGGAGTGAAAAAA

Downstream 100 bases:

>100_bases
GGGGGAAGAACAATGCCAGTTATGTCATATATAGATGCTGTCACTTTGGCTTTGAAAGAAGAAATGGAAAAGGATCCCCG
CGTCTTTGTTTTAGGGGAGG

Product: BkdAA

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; BCKDH E1-alpha [H]

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MGNNRHEAVGLTDEQAIDMYKTMLLARKLDERMWLLNRSGKIPFVISCQGQEAAQVGAAFALDRENDYVLPYYRDMGVVL
AFGMTAKDLMLSAFAKAEDPNSGGKQMPSHFGQKKNRIVTGSSPVTTQVPHAVGVALAGRLDKKDIATFVTFGEGSSNQG
DFHEGANFAAVHKLPVIFMCENNKYAISVPYEKQVACENISDRAVGYGMPGVTVDGNDPLEVYKAVKEARERAGRGEGPT
LIETISYRLTPHSSDDDDRSYREREEVAEAKKNDPLNKFAQYLKEAGLLDDQSEKQISDEITKIVNEATDYAEHAPYADP
EDALRHVYAE

Sequences:

>Translated_330_residues
MGNNRHEAVGLTDEQAIDMYKTMLLARKLDERMWLLNRSGKIPFVISCQGQEAAQVGAAFALDRENDYVLPYYRDMGVVL
AFGMTAKDLMLSAFAKAEDPNSGGKQMPSHFGQKKNRIVTGSSPVTTQVPHAVGVALAGRLDKKDIATFVTFGEGSSNQG
DFHEGANFAAVHKLPVIFMCENNKYAISVPYEKQVACENISDRAVGYGMPGVTVDGNDPLEVYKAVKEARERAGRGEGPT
LIETISYRLTPHSSDDDDRSYREREEVAEAKKNDPLNKFAQYLKEAGLLDDQSEKQISDEITKIVNEATDYAEHAPYADP
EDALRHVYAE
>Mature_329_residues
GNNRHEAVGLTDEQAIDMYKTMLLARKLDERMWLLNRSGKIPFVISCQGQEAAQVGAAFALDRENDYVLPYYRDMGVVLA
FGMTAKDLMLSAFAKAEDPNSGGKQMPSHFGQKKNRIVTGSSPVTTQVPHAVGVALAGRLDKKDIATFVTFGEGSSNQGD
FHEGANFAAVHKLPVIFMCENNKYAISVPYEKQVACENISDRAVGYGMPGVTVDGNDPLEVYKAVKEARERAGRGEGPTL
IETISYRLTPHSSDDDDRSYREREEVAEAKKNDPLNKFAQYLKEAGLLDDQSEKQISDEITKIVNEATDYAEHAPYADPE
DALRHVYAE

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCKDHA family [H]

Homologues:

Organism=Homo sapiens, GI11386135, Length=321, Percent_Identity=38.3177570093458, Blast_Score=217, Evalue=1e-56,
Organism=Homo sapiens, GI258645172, Length=321, Percent_Identity=38.3177570093458, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI4885543, Length=321, Percent_Identity=31.4641744548287, Blast_Score=148, Evalue=8e-36,
Organism=Homo sapiens, GI291084742, Length=321, Percent_Identity=31.4641744548287, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI4505685, Length=321, Percent_Identity=31.4641744548287, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI291084744, Length=328, Percent_Identity=30.7926829268293, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI291084757, Length=321, Percent_Identity=28.0373831775701, Blast_Score=106, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI86563355, Length=322, Percent_Identity=40.0621118012422, Blast_Score=217, Evalue=8e-57,
Organism=Caenorhabditis elegans, GI86563357, Length=322, Percent_Identity=40.0621118012422, Blast_Score=217, Evalue=8e-57,
Organism=Caenorhabditis elegans, GI17536047, Length=328, Percent_Identity=32.0121951219512, Blast_Score=147, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI32564172, Length=328, Percent_Identity=32.0121951219512, Blast_Score=146, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6321026, Length=319, Percent_Identity=31.0344827586207, Blast_Score=139, Evalue=5e-34,
Organism=Drosophila melanogaster, GI21355903, Length=315, Percent_Identity=34.2857142857143, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24639740, Length=329, Percent_Identity=32.2188449848024, Blast_Score=141, Evalue=7e-34,
Organism=Drosophila melanogaster, GI24639744, Length=329, Percent_Identity=32.2188449848024, Blast_Score=140, Evalue=9e-34,
Organism=Drosophila melanogaster, GI28571106, Length=329, Percent_Identity=32.2188449848024, Blast_Score=140, Evalue=9e-34,
Organism=Drosophila melanogaster, GI24639746, Length=287, Percent_Identity=32.7526132404181, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24639748, Length=306, Percent_Identity=32.0261437908497, Blast_Score=128, Evalue=7e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 36463; Mature: 36332

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNNRHEAVGLTDEQAIDMYKTMLLARKLDERMWLLNRSGKIPFVISCQGQEAAQVGAAF
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHE
ALDRENDYVLPYYRDMGVVLAFGMTAKDLMLSAFAKAEDPNSGGKQMPSHFGQKKNRIVT
EECCCCCEEECHHHCCCCHHEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHCHHCCEEEE
GSSPVTTQVPHAVGVALAGRLDKKDIATFVTFGEGSSNQGDFHEGANFAAVHKLPVIFMC
CCCCCCCCCCHHHHHHHHCCCCHHHHEEEEEECCCCCCCCCHHCCCCEEEEECCCEEEEE
ENNKYAISVPYEKQVACENISDRAVGYGMPGVTVDGNDPLEVYKAVKEARERAGRGEGPT
CCCCEEEECCCHHHHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCH
LIETISYRLTPHSSDDDDRSYREREEVAEAKKNDPLNKFAQYLKEAGLLDDQSEKQISDE
HHHHHEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHH
ITKIVNEATDYAEHAPYADPEDALRHVYAE
HHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
GNNRHEAVGLTDEQAIDMYKTMLLARKLDERMWLLNRSGKIPFVISCQGQEAAQVGAAF
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHE
ALDRENDYVLPYYRDMGVVLAFGMTAKDLMLSAFAKAEDPNSGGKQMPSHFGQKKNRIVT
EECCCCCEEECHHHCCCCHHEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHCHHCCEEEE
GSSPVTTQVPHAVGVALAGRLDKKDIATFVTFGEGSSNQGDFHEGANFAAVHKLPVIFMC
CCCCCCCCCCHHHHHHHHCCCCHHHHEEEEEECCCCCCCCCHHCCCCEEEEECCCEEEEE
ENNKYAISVPYEKQVACENISDRAVGYGMPGVTVDGNDPLEVYKAVKEARERAGRGEGPT
CCCCEEEECCCHHHHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCH
LIETISYRLTPHSSDDDDRSYREREEVAEAKKNDPLNKFAQYLKEAGLLDDQSEKQISDE
HHHHHEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHH
ITKIVNEATDYAEHAPYADPEDALRHVYAE
HHHHHHHHHHHHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8504804; 8969508; 9384377 [H]