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The map label for this gene is bkdAB [H]

Identifier: 52786319

GI number: 52786319

Start: 2499152

End: 2500135

Strand: Reverse

Name: bkdAB [H]

Synonym: BLi02581

Alternate gene names: 52786319

Gene position: 2500135-2499152 (Counterclockwise)

Preceding gene: 52786320

Following gene: 52786318

Centisome position: 59.21

GC content: 52.13

Gene sequence:

>984_bases
ATGCCAGTTATGTCATATATAGATGCTGTCACTTTGGCTTTGAAAGAAGAAATGGAAAAGGATCCCCGCGTCTTTGTTTT
AGGGGAGGATGTCGGCAAAAAAGGCGGTGTATTCAAGGCGACAGCCGGTCTTTACGACCAGTTCGGCGAAGAGCGGGTGA
TGGACACGCCTTTGGCGGAATCCGCAATTGCCGGCGTCGGAATCGGGGCCGCCATGTACGGGATGAGACCAGTCGCTGAA
ATGCAGTTTGCTGATTTTATTATGCCGGCTGTCAACCAGATTATTTCGGAAGCGGCAAAAATCCGCTACCGCTCCAACAA
TGACTGGAACTGTCCGATCGTCATCCGCGCACCTTACGGCGGAGGCGTGCACGGCGCGCTGTACCATTCACAGTCGGTTG
AAGCCGTGTTTGCCAATCAGCCCGGCCTGAAAATCGTCATGCCTTCGACGCCTTATGATGTCAAAGGGCTTTTGAAAGCG
GCCATCCGCGATGACGACCCTGTGCTGTTCTTCGAACATAAACGGGCATACCGGCTGATCAAGGGCGAAGTGCCGAGTGA
CGACTATGTGCTGCCGATCGGAAAGGCGGACGTGAAGCGGGAAGGCGAAGACATTACCGTCATCACATACGGATTGTGCG
TCCATTTCGCCCTTCAGGCTGCCGAACGGCTTGCCAAAGACGGGATTTCCGCACATATCCTTGATTTAAGAACGGTATAT
CCGCTCGATCAGGAAGCGATTATTGAAGCGGCTTCCAAAACGGGCAAAGTGCTTTTGATGACCGAAGATACGAAGGAAGG
CAGCATTATGAGTGAAGTGGCCGCGATCATTTCAGAGCATTGCCTGTTCGATTTGGATGCGCCGATTAAGCGGTTGGCGG
GCCCTGATGTCCCGGCAATGCCGTATGCTCCTACAATGGAAAAATTCTTTATGGTCAATCCGGATAAAGCAGAAGCAGCG
ATGAGAGAATTGGCTGAGTTTTAA

Upstream 100 bases:

>100_bases
AAAAATCGTCAATGAAGCAACAGATTATGCTGAACATGCACCTTATGCAGATCCGGAAGATGCGCTGCGGCATGTTTATG
CGGAGTAAGGGGGAAGAACA

Downstream 100 bases:

>100_bases
TATAGCGAAGGAGGACACATCGTATGGCAGTGGAACAAATGACAATGCCGCAGCTCGGGGAGAGCGTGACTGAGGGAACC
ATCAGCAAATGGCTCGTCAG

Product: BkdAB

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component beta chain; BCKDH E1-beta [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MPVMSYIDAVTLALKEEMEKDPRVFVLGEDVGKKGGVFKATAGLYDQFGEERVMDTPLAESAIAGVGIGAAMYGMRPVAE
MQFADFIMPAVNQIISEAAKIRYRSNNDWNCPIVIRAPYGGGVHGALYHSQSVEAVFANQPGLKIVMPSTPYDVKGLLKA
AIRDDDPVLFFEHKRAYRLIKGEVPSDDYVLPIGKADVKREGEDITVITYGLCVHFALQAAERLAKDGISAHILDLRTVY
PLDQEAIIEAASKTGKVLLMTEDTKEGSIMSEVAAIISEHCLFDLDAPIKRLAGPDVPAMPYAPTMEKFFMVNPDKAEAA
MRELAEF

Sequences:

>Translated_327_residues
MPVMSYIDAVTLALKEEMEKDPRVFVLGEDVGKKGGVFKATAGLYDQFGEERVMDTPLAESAIAGVGIGAAMYGMRPVAE
MQFADFIMPAVNQIISEAAKIRYRSNNDWNCPIVIRAPYGGGVHGALYHSQSVEAVFANQPGLKIVMPSTPYDVKGLLKA
AIRDDDPVLFFEHKRAYRLIKGEVPSDDYVLPIGKADVKREGEDITVITYGLCVHFALQAAERLAKDGISAHILDLRTVY
PLDQEAIIEAASKTGKVLLMTEDTKEGSIMSEVAAIISEHCLFDLDAPIKRLAGPDVPAMPYAPTMEKFFMVNPDKAEAA
MRELAEF
>Mature_326_residues
PVMSYIDAVTLALKEEMEKDPRVFVLGEDVGKKGGVFKATAGLYDQFGEERVMDTPLAESAIAGVGIGAAMYGMRPVAEM
QFADFIMPAVNQIISEAAKIRYRSNNDWNCPIVIRAPYGGGVHGALYHSQSVEAVFANQPGLKIVMPSTPYDVKGLLKAA
IRDDDPVLFFEHKRAYRLIKGEVPSDDYVLPIGKADVKREGEDITVITYGLCVHFALQAAERLAKDGISAHILDLRTVYP
LDQEAIIEAASKTGKVLLMTEDTKEGSIMSEVAAIISEHCLFDLDAPIKRLAGPDVPAMPYAPTMEKFFMVNPDKAEAAM
RELAEF

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4557353, Length=327, Percent_Identity=42.8134556574924, Blast_Score=279, Evalue=3e-75,
Organism=Homo sapiens, GI34101272, Length=327, Percent_Identity=42.8134556574924, Blast_Score=279, Evalue=3e-75,
Organism=Homo sapiens, GI156564403, Length=304, Percent_Identity=39.1447368421053, Blast_Score=231, Evalue=7e-61,
Organism=Homo sapiens, GI291084858, Length=304, Percent_Identity=37.828947368421, Blast_Score=213, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17538422, Length=309, Percent_Identity=43.042071197411, Blast_Score=252, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI17506935, Length=316, Percent_Identity=42.4050632911392, Blast_Score=245, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6319698, Length=312, Percent_Identity=40.0641025641026, Blast_Score=236, Evalue=3e-63,
Organism=Drosophila melanogaster, GI160714832, Length=327, Percent_Identity=41.2844036697248, Blast_Score=256, Evalue=1e-68,
Organism=Drosophila melanogaster, GI160714828, Length=327, Percent_Identity=41.2844036697248, Blast_Score=256, Evalue=1e-68,
Organism=Drosophila melanogaster, GI21358145, Length=308, Percent_Identity=42.2077922077922, Blast_Score=248, Evalue=6e-66,
Organism=Drosophila melanogaster, GI24650940, Length=308, Percent_Identity=42.2077922077922, Blast_Score=248, Evalue=6e-66,
Organism=Drosophila melanogaster, GI24650943, Length=90, Percent_Identity=43.3333333333333, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24650945, Length=90, Percent_Identity=43.3333333333333, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 35777; Mature: 35646

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVMSYIDAVTLALKEEMEKDPRVFVLGEDVGKKGGVFKATAGLYDQFGEERVMDTPLAE
CCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEHHHHHHHHCCCHHCCCCHHH
SAIAGVGIGAAMYGMRPVAEMQFADFIMPAVNQIISEAAKIRYRSNNDWNCPIVIRAPYG
HHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEEECCCC
GGVHGALYHSQSVEAVFANQPGLKIVMPSTPYDVKGLLKAAIRDDDPVLFFEHKRAYRLI
CCCCHHEECCCCCEEEECCCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEECCCHHHHH
KGEVPSDDYVLPIGKADVKREGEDITVITYGLCVHFALQAAERLAKDGISAHILDLRTVY
CCCCCCCCEEEECCCHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEEHHEEC
PLDQEAIIEAASKTGKVLLMTEDTKEGSIMSEVAAIISEHCLFDLDAPIKRLAGPDVPAM
CCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHEECCCHHHHHHCCCCCCCC
PYAPTMEKFFMVNPDKAEAAMRELAEF
CCCCCCHHEEEECCCHHHHHHHHHHCC
>Mature Secondary Structure 
PVMSYIDAVTLALKEEMEKDPRVFVLGEDVGKKGGVFKATAGLYDQFGEERVMDTPLAE
CHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEHHHHHHHHCCCHHCCCCHHH
SAIAGVGIGAAMYGMRPVAEMQFADFIMPAVNQIISEAAKIRYRSNNDWNCPIVIRAPYG
HHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEEECCCC
GGVHGALYHSQSVEAVFANQPGLKIVMPSTPYDVKGLLKAAIRDDDPVLFFEHKRAYRLI
CCCCHHEECCCCCEEEECCCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEECCCHHHHH
KGEVPSDDYVLPIGKADVKREGEDITVITYGLCVHFALQAAERLAKDGISAHILDLRTVY
CCCCCCCCEEEECCCHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEEHHEEC
PLDQEAIIEAASKTGKVLLMTEDTKEGSIMSEVAAIISEHCLFDLDAPIKRLAGPDVPAM
CCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHEECCCHHHHHHCCCCCCCC
PYAPTMEKFFMVNPDKAEAAMRELAEF
CCCCCCHHEEEECCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8504804; 8969508; 9384377 [H]