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The map label for this gene is dacF [H]

Identifier: 52786237

GI number: 52786237

Start: 2427409

End: 2428581

Strand: Reverse

Name: dacF [H]

Synonym: BLi02498

Alternate gene names: 52786237

Gene position: 2428581-2427409 (Counterclockwise)

Preceding gene: 52786238

Following gene: 52786236

Centisome position: 57.51

GC content: 44.59

Gene sequence:

>1173_bases
ATGAAACGTCATTTATCCAAATTGCTGATTTTTACGATAATCCTGATGACTGCGCCGTCGGCGCTTGCAAAAGAGAGCGC
AAAGAAACCGTCGGAGCTTGCTGATGACGCAAAATCTGCCGTTTTAATCGAGCGGGATACCGGTAAGGTGATGTATGACA
AGAACAGCCGTGAAAAGCTTCCTCCGGCCAGCATGACAAAGGTGATGACCATGCTGCTGATCATGGAGGCCCTTGATGAA
GGCAAAATCAAAATGAAAGACAAAGTCAGAACAAGCGAGTATGCCGCTTCAATGGGGGGTTCGCAAATTTTTCTAGAGCC
CGGTGAAGAAATGACGGTTGAAGATATGCTGAAAGGGATCGCCATCGGATCGGGTAATGATGCCTCTGTTGCAATGGCAG
AGCATATTGCGGGGTCTGAAGAGGAATTCGTCAAAAGAATGAATGAAAAAGCTGCTGAGCTCGGCCTCAAGGATACGTCT
TTTCAAAATACGACAGGTCTGCCTGAAGAAGGCCATTACAGCACAGCCTATGACATGGCGCTCATGGCGAAAGAGCTTTT
AAAATATGAAAACATCACGAAATTTACGGGAACGTATGAAGACTATCTGAGAGAAAACACTGATAAAAAATTCTGGCTCG
TCAACACAAACCGCCTCATCAAATTTTATCCAGGAGTTGATGGAGTCAAGACCGGCTTTACAAATGAAGCAAAGTACTGT
CTGACAGCTTCAGCGAAAAAAGGAAACATGCGCGTCATCGCTGTCGTTTTTGGAGCCAGCACGCCGAAAGAACGAAATGC
CCAAATCACAAAAATGCTTGATTTTGCGTTCAGCCAATATGAAACGCATCCGATGTATAAACGTAACGAAATCATTTCCG
ATTTGAAGGTCAATAAAGGCCGTGAGCAAAAAATAAACCTTGTCACATCAGAACCGATTTCCCTTTTGACGAAAAAAGGC
GAAAATATCGAATCTGTTAAAAAAGAAATCAAACAGAAAGAAGATATCCAGGCCCCTGTCAAAAAAGGCACAGAGCTTGG
GACGCTCGTTTTGAAAAAGGATGGAAAGGTGCTCGCTGAAAGTCCTCTTGTCGCTGAAAAAGATATGGACAAAGCGGGGA
TGTGGACGATGTTCAAGCGGACGATGACCCACTGGACGAAGTGGAGTGAATAA

Upstream 100 bases:

>100_bases
CTCTTTCTTTTTGGTCGTTGAGAAATTTTTTCCAACCGATCCGTATAAATCCAACCGGATTGGAAAAAATAGAGAGGATT
ACGAAATGGAGGTCTGGGTG

Downstream 100 bases:

>100_bases
TGCCGAACGGTCACTAGTTTTGTCACGGTGAAGGAATTTATAAAGTCTGAAGCGAAACACTCATTATCCGATTTAAACCA
AGGAGGAATGAGGATGAGCC

Product: DacF

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MKRHLSKLLIFTIILMTAPSALAKESAKKPSELADDAKSAVLIERDTGKVMYDKNSREKLPPASMTKVMTMLLIMEALDE
GKIKMKDKVRTSEYAASMGGSQIFLEPGEEMTVEDMLKGIAIGSGNDASVAMAEHIAGSEEEFVKRMNEKAAELGLKDTS
FQNTTGLPEEGHYSTAYDMALMAKELLKYENITKFTGTYEDYLRENTDKKFWLVNTNRLIKFYPGVDGVKTGFTNEAKYC
LTASAKKGNMRVIAVVFGASTPKERNAQITKMLDFAFSQYETHPMYKRNEIISDLKVNKGREQKINLVTSEPISLLTKKG
ENIESVKKEIKQKEDIQAPVKKGTELGTLVLKKDGKVLAESPLVAEKDMDKAGMWTMFKRTMTHWTKWSE

Sequences:

>Translated_390_residues
MKRHLSKLLIFTIILMTAPSALAKESAKKPSELADDAKSAVLIERDTGKVMYDKNSREKLPPASMTKVMTMLLIMEALDE
GKIKMKDKVRTSEYAASMGGSQIFLEPGEEMTVEDMLKGIAIGSGNDASVAMAEHIAGSEEEFVKRMNEKAAELGLKDTS
FQNTTGLPEEGHYSTAYDMALMAKELLKYENITKFTGTYEDYLRENTDKKFWLVNTNRLIKFYPGVDGVKTGFTNEAKYC
LTASAKKGNMRVIAVVFGASTPKERNAQITKMLDFAFSQYETHPMYKRNEIISDLKVNKGREQKINLVTSEPISLLTKKG
ENIESVKKEIKQKEDIQAPVKKGTELGTLVLKKDGKVLAESPLVAEKDMDKAGMWTMFKRTMTHWTKWSE
>Mature_390_residues
MKRHLSKLLIFTIILMTAPSALAKESAKKPSELADDAKSAVLIERDTGKVMYDKNSREKLPPASMTKVMTMLLIMEALDE
GKIKMKDKVRTSEYAASMGGSQIFLEPGEEMTVEDMLKGIAIGSGNDASVAMAEHIAGSEEEFVKRMNEKAAELGLKDTS
FQNTTGLPEEGHYSTAYDMALMAKELLKYENITKFTGTYEDYLRENTDKKFWLVNTNRLIKFYPGVDGVKTGFTNEAKYC
LTASAKKGNMRVIAVVFGASTPKERNAQITKMLDFAFSQYETHPMYKRNEIISDLKVNKGREQKINLVTSEPISLLTKKG
ENIESVKKEIKQKEDIQAPVKKGTELGTLVLKKDGKVLAESPLVAEKDMDKAGMWTMFKRTMTHWTKWSE

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=390, Percent_Identity=34.6153846153846, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI1786851, Length=366, Percent_Identity=33.3333333333333, Blast_Score=188, Evalue=4e-49,
Organism=Escherichia coli, GI87082022, Length=394, Percent_Identity=29.9492385786802, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI87082054, Length=226, Percent_Identity=32.7433628318584, Blast_Score=89, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 43752; Mature: 43752

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRHLSKLLIFTIILMTAPSALAKESAKKPSELADDAKSAVLIERDTGKVMYDKNSREKL
CHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHCEEEEEECCCCEEECCCCCCCC
PPASMTKVMTMLLIMEALDEGKIKMKDKVRTSEYAASMGGSQIFLEPGEEMTVEDMLKGI
CCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHE
AIGSGNDASVAMAEHIAGSEEEFVKRMNEKAAELGLKDTSFQNTTGLPEEGHYSTAYDMA
ECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHH
LMAKELLKYENITKFTGTYEDYLRENTDKKFWLVNTNRLIKFYPGVDGVKTGFTNEAKYC
HHHHHHHHHHCCHHHCCCHHHHHHCCCCCEEEEEECCCEEEEECCCCCHHCCCCCCCCEE
LTASAKKGNMRVIAVVFGASTPKERNAQITKMLDFAFSQYETHPMYKRNEIISDLKVNKG
EEECCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
REQKINLVTSEPISLLTKKGENIESVKKEIKQKEDIQAPVKKGTELGTLVLKKDGKVLAE
CCCEEEEEECCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEC
SPLVAEKDMDKAGMWTMFKRTMTHWTKWSE
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKRHLSKLLIFTIILMTAPSALAKESAKKPSELADDAKSAVLIERDTGKVMYDKNSREKL
CHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHCEEEEEECCCCEEECCCCCCCC
PPASMTKVMTMLLIMEALDEGKIKMKDKVRTSEYAASMGGSQIFLEPGEEMTVEDMLKGI
CCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHE
AIGSGNDASVAMAEHIAGSEEEFVKRMNEKAAELGLKDTSFQNTTGLPEEGHYSTAYDMA
ECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHH
LMAKELLKYENITKFTGTYEDYLRENTDKKFWLVNTNRLIKFYPGVDGVKTGFTNEAKYC
HHHHHHHHHHCCHHHCCCHHHHHHCCCCCEEEEEECCCEEEEECCCCCHHCCCCCCCCEE
LTASAKKGNMRVIAVVFGASTPKERNAQITKMLDFAFSQYETHPMYKRNEIISDLKVNKG
EEECCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
REQKINLVTSEPISLLTKKGENIESVKKEIKQKEDIQAPVKKGTELGTLVLKKDGKVLAE
CCCEEEEEECCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEC
SPLVAEKDMDKAGMWTMFKRTMTHWTKWSE
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]