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The map label for this gene is punA [H]

Identifier: 52786238

GI number: 52786238

Start: 2428739

End: 2429554

Strand: Reverse

Name: punA [H]

Synonym: BLi02499

Alternate gene names: 52786238

Gene position: 2429554-2428739 (Counterclockwise)

Preceding gene: 52786239

Following gene: 52786237

Centisome position: 57.54

GC content: 50.98

Gene sequence:

>816_bases
GTGAAACAGATGATTGCAAATGCCGCAGACTATATTAAAAGCAAAACGGAAATCAAACCTGCGGTCGGCCTGATATTGGG
ATCCGGCCTTGGCGTGCTTGCCGATGAGATAGAAGACGCCGTCCGCATTAAATATGAAGACATCCCGGACTTTCCTGTTT
CCACTGTTGAAGGGCATGCCGGACAGCTTGTCATCGGCAGACTTGAAAATGTGCAAGTCGTCGCGATGCAAGGGCGTTTT
CATTTTTACGAAGGGTATTCCATGGACAAGGTAACCTTCCCTGTCCGTGTCATGAAAGAGCTGGGGATCGAGACTTTAAT
CGTGACCAATGCCGCAGGCGGCGTCAATGAATCGTTCCGTCCCGGCGATTTGATGATCATATCCGATCATATCAATTATA
TGGGAACGAACCCGCTGATCGGTCCAAACGATTCCTCGCTCGGCGTCAGATTCCCGGATATGTCGTCCGCATATGATAAG
GAACTGCGCCTCTTGGCGAAAAAAACAGCCGAAGAATTGAACATCAGCGTACAAGAAGGGGTTTACACAGCCGTTACAGG
ACCTACATACGAAACGCCTGCCGAAGTGCGGTTCCTCAGAACGATCGGATCGGATGCCGTCGGCATGTCGACGGTTCCGG
AAGTCATCGTGGCAAAACATGCGGGCCTTCGCGTGCTCGGCATTTCGTGTATATCAAACGCGGCAGCCGGAATCCTCGAT
CAGCCGCTCTCCCATGATGAAGTGATCGAAGTCACCGAAAAAGTAAAAGCCGACTTCTTGCGGTTTGTTAAAGCGGCAGT
CGCCAAAATCGGCTAA

Upstream 100 bases:

>100_bases
CATTCGCGGACATCGGCGCAACGATCGCCGACAACTTTAAAACGAATATGCCAAAGTACGGAAAAAGCTTTTTATCCCTG
CTGAAATAGGAGGAAACGAC

Downstream 100 bases:

>100_bases
AACTACAGCGAAGTTGAAATATGAGCCAGTGCAAGTTCAAATGAGAGCCTGCATTGGCTCTTTCTTTTTGGTCGTTGAGA
AATTTTTTCCAACCGATCCG

Product: purine nucleoside phosphorylase

Products: NA

Alternate protein names: Inosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; PU-NPase I [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MKQMIANAADYIKSKTEIKPAVGLILGSGLGVLADEIEDAVRIKYEDIPDFPVSTVEGHAGQLVIGRLENVQVVAMQGRF
HFYEGYSMDKVTFPVRVMKELGIETLIVTNAAGGVNESFRPGDLMIISDHINYMGTNPLIGPNDSSLGVRFPDMSSAYDK
ELRLLAKKTAEELNISVQEGVYTAVTGPTYETPAEVRFLRTIGSDAVGMSTVPEVIVAKHAGLRVLGISCISNAAAGILD
QPLSHDEVIEVTEKVKADFLRFVKAAVAKIG

Sequences:

>Translated_271_residues
MKQMIANAADYIKSKTEIKPAVGLILGSGLGVLADEIEDAVRIKYEDIPDFPVSTVEGHAGQLVIGRLENVQVVAMQGRF
HFYEGYSMDKVTFPVRVMKELGIETLIVTNAAGGVNESFRPGDLMIISDHINYMGTNPLIGPNDSSLGVRFPDMSSAYDK
ELRLLAKKTAEELNISVQEGVYTAVTGPTYETPAEVRFLRTIGSDAVGMSTVPEVIVAKHAGLRVLGISCISNAAAGILD
QPLSHDEVIEVTEKVKADFLRFVKAAVAKIG
>Mature_271_residues
MKQMIANAADYIKSKTEIKPAVGLILGSGLGVLADEIEDAVRIKYEDIPDFPVSTVEGHAGQLVIGRLENVQVVAMQGRF
HFYEGYSMDKVTFPVRVMKELGIETLIVTNAAGGVNESFRPGDLMIISDHINYMGTNPLIGPNDSSLGVRFPDMSSAYDK
ELRLLAKKTAEELNISVQEGVYTAVTGPTYETPAEVRFLRTIGSDAVGMSTVPEVIVAKHAGLRVLGISCISNAAAGILD
QPLSHDEVIEVTEKVKADFLRFVKAAVAKIG

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=273, Percent_Identity=50.1831501831502, Blast_Score=265, Evalue=4e-71,
Organism=Homo sapiens, GI47132622, Length=243, Percent_Identity=30.4526748971193, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1788746, Length=262, Percent_Identity=47.7099236641221, Blast_Score=246, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI17541190, Length=256, Percent_Identity=45.3125, Blast_Score=222, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6323238, Length=301, Percent_Identity=42.5249169435216, Blast_Score=211, Evalue=7e-56,
Organism=Drosophila melanogaster, GI45552887, Length=277, Percent_Identity=46.9314079422383, Blast_Score=254, Evalue=6e-68,
Organism=Drosophila melanogaster, GI45552885, Length=277, Percent_Identity=46.9314079422383, Blast_Score=253, Evalue=7e-68,
Organism=Drosophila melanogaster, GI24656093, Length=277, Percent_Identity=46.9314079422383, Blast_Score=253, Evalue=8e-68,
Organism=Drosophila melanogaster, GI24656090, Length=277, Percent_Identity=46.9314079422383, Blast_Score=253, Evalue=9e-68,
Organism=Drosophila melanogaster, GI24762376, Length=233, Percent_Identity=40.343347639485, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI221459247, Length=230, Percent_Identity=26.0869565217391, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI20130079, Length=225, Percent_Identity=28.4444444444444, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011270
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 29224; Mature: 29224

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS01240 PNP_MTAP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQMIANAADYIKSKTEIKPAVGLILGSGLGVLADEIEDAVRIKYEDIPDFPVSTVEGHA
CHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHEEEECCCCCCCCCCCCCCC
GQLVIGRLENVQVVAMQGRFHFYEGYSMDKVTFPVRVMKELGIETLIVTNAAGGVNESFR
CEEEEEECCCEEEEEEECCEEEECCCCCCCEEHHHHHHHHCCCEEEEEECCCCCCCCCCC
PGDLMIISDHINYMGTNPLIGPNDSSLGVRFPDMSSAYDKELRLLAKKTAEELNISVQEG
CCCEEEEECCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCEEHHCC
VYTAVTGPTYETPAEVRFLRTIGSDAVGMSTVPEVIVAKHAGLRVLGISCISNAAAGILD
EEEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHC
QPLSHDEVIEVTEKVKADFLRFVKAAVAKIG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKQMIANAADYIKSKTEIKPAVGLILGSGLGVLADEIEDAVRIKYEDIPDFPVSTVEGHA
CHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHEEEECCCCCCCCCCCCCCC
GQLVIGRLENVQVVAMQGRFHFYEGYSMDKVTFPVRVMKELGIETLIVTNAAGGVNESFR
CEEEEEECCCEEEEEEECCEEEECCCCCCCEEHHHHHHHHCCCEEEEEECCCCCCCCCCC
PGDLMIISDHINYMGTNPLIGPNDSSLGVRFPDMSSAYDKELRLLAKKTAEELNISVQEG
CCCEEEEECCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCEEHHCC
VYTAVTGPTYETPAEVRFLRTIGSDAVGMSTVPEVIVAKHAGLRVLGISCISNAAAGILD
EEEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHC
QPLSHDEVIEVTEKVKADFLRFVKAAVAKIG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10537218; 8969508; 9384377; 1629150 [H]