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The map label for this gene is trpA

Identifier: 52786142

GI number: 52786142

Start: 2342196

End: 2342999

Strand: Reverse

Name: trpA

Synonym: BLi02398

Alternate gene names: 52786142

Gene position: 2342999-2342196 (Counterclockwise)

Preceding gene: 52786143

Following gene: 52786141

Centisome position: 55.49

GC content: 48.01

Gene sequence:

>804_bases
ATGTTCAATCTGAAAAAACAGCCGTCCGATAAGCTATTTGTTCCGTTTATTACAGCGGGAGACCCGCTGCCAGAAATCTC
GATCGAGCTGGCAAAATCTCTTCAAGAGGCGGGAGCGTCCGCCCTTGAACTCGGGGTTCCGTATTCTGATCCGCTCGCTG
ACGGCCCGGTGATTCAGCGCGCATCAAAAAGGGCGCTTGACAACGATATGAACATCGTAAAAGCGATCAGGCTTGGCGGT
GAAATGAAAAAAAACGGCGTCCATATTCCGATTATTCTCTTTACGTATTATAATCCTGTGTTACAATTAGATACAGATCA
CTTTTTCGCTTTACTGCGACAAAATCAGCTAGACGGACTCCTTGTACCGGACCTTCCTTTTGAGGAAAGCGTATATTTGC
AGCAGCAGTGTAAAAAACACGGGATTACCTATATTTCGCTGGTCGCGCCGACAAGCGAATCGCGCATTCAAAAAATAACG
CAGCAGGCGGAAGGTTTTGTGTATTGCGTTTCTTCGCTCGGTGTCACAGGTGTGCGCAATGAATTCGAAGATTCTGTTTC
TTCATTTATCAGGTCTGTTAAAGGAATGAGCTCCGTCCCGGTTGCCGTCGGGTTTGGGATTTCCAGCTCCAAACAAGTGG
AGATGATGAATGAACTGGCGGACGGCGTTGTCGTGGGAAGCGCCCTTGTCAGAAAGATCGAGGAGCTGAAAGACGAACTC
GTCGCCCCTGAGACAAGGGAACAAGCCTTAAAGGCGTTTGAGGCATACGCTAAGACATTTAGCGGATCGTACATGGTAAA
ATGA

Upstream 100 bases:

>100_bases
GAACTGCCGAGAGAGAAAAGCGTTCTCGTCTGCCTTTCAGGCAGGGGAGACAAAGACGTCCATACCTTGATGAAGGTGTT
GGAAGAAGAGGTGAATCAAG

Downstream 100 bases:

>100_bases
GGTGACAGAATGTTGCAAATTAAAGAACAGTTAAAACAATTAACGCCTTACCAGCCGGGCAAACCGATTGAAGAGGTCAA
AAAAGAGTTCAACCTCGACC

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MFNLKKQPSDKLFVPFITAGDPLPEISIELAKSLQEAGASALELGVPYSDPLADGPVIQRASKRALDNDMNIVKAIRLGG
EMKKNGVHIPIILFTYYNPVLQLDTDHFFALLRQNQLDGLLVPDLPFEESVYLQQQCKKHGITYISLVAPTSESRIQKIT
QQAEGFVYCVSSLGVTGVRNEFEDSVSSFIRSVKGMSSVPVAVGFGISSSKQVEMMNELADGVVVGSALVRKIEELKDEL
VAPETREQALKAFEAYAKTFSGSYMVK

Sequences:

>Translated_267_residues
MFNLKKQPSDKLFVPFITAGDPLPEISIELAKSLQEAGASALELGVPYSDPLADGPVIQRASKRALDNDMNIVKAIRLGG
EMKKNGVHIPIILFTYYNPVLQLDTDHFFALLRQNQLDGLLVPDLPFEESVYLQQQCKKHGITYISLVAPTSESRIQKIT
QQAEGFVYCVSSLGVTGVRNEFEDSVSSFIRSVKGMSSVPVAVGFGISSSKQVEMMNELADGVVVGSALVRKIEELKDEL
VAPETREQALKAFEAYAKTFSGSYMVK
>Mature_267_residues
MFNLKKQPSDKLFVPFITAGDPLPEISIELAKSLQEAGASALELGVPYSDPLADGPVIQRASKRALDNDMNIVKAIRLGG
EMKKNGVHIPIILFTYYNPVLQLDTDHFFALLRQNQLDGLLVPDLPFEESVYLQQQCKKHGITYISLVAPTSESRIQKIT
QQAEGFVYCVSSLGVTGVRNEFEDSVSSFIRSVKGMSSVPVAVGFGISSSKQVEMMNELADGVVVGSALVRKIEELKDEL
VAPETREQALKAFEAYAKTFSGSYMVK

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=255, Percent_Identity=34.9019607843137, Blast_Score=145, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6321412, Length=230, Percent_Identity=35.6521739130435, Blast_Score=159, Evalue=6e-40,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_BACLD (Q65I36)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_079562.1
- RefSeq:   YP_091971.1
- ProteinModelPortal:   Q65I36
- SMR:   Q65I36
- STRING:   Q65I36
- EnsemblBacteria:   EBBACT00000054873
- EnsemblBacteria:   EBBACT00000061124
- GeneID:   3028566
- GeneID:   3099453
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi02398
- KEGG:   bli:BL02770
- NMPDR:   fig|279010.5.peg.248
- eggNOG:   COG0159
- GeneTree:   EBGT00050000001942
- HOGENOM:   HBG391690
- OMA:   VHIPIIL
- ProtClustDB:   PRK13111
- BioCyc:   BLIC279010-1:BLI02398-MONOMER
- BioCyc:   BLIC279010:BL02770-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 29294; Mature: 29294

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 43-43 ACT_SITE 54-54

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNLKKQPSDKLFVPFITAGDPLPEISIELAKSLQEAGASALELGVPYSDPLADGPVIQR
CCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHH
ASKRALDNDMNIVKAIRLGGEMKKNGVHIPIILFTYYNPVLQLDTDHFFALLRQNQLDGL
HHHHHHCCHHHHHHHHHHCCCHHHCCEEEEEEEEEECCCEEEECHHHHHHHHHHCCCCCE
LVPDLPFEESVYLQQQCKKHGITYISLVAPTSESRIQKITQQAEGFVYCVSSLGVTGVRN
ECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCEEEHHHCCCCCCHH
EFEDSVSSFIRSVKGMSSVPVAVGFGISSSKQVEMMNELADGVVVGSALVRKIEELKDEL
HHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAPETREQALKAFEAYAKTFSGSYMVK
CCCHHHHHHHHHHHHHHHHHCCCEECC
>Mature Secondary Structure
MFNLKKQPSDKLFVPFITAGDPLPEISIELAKSLQEAGASALELGVPYSDPLADGPVIQR
CCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHH
ASKRALDNDMNIVKAIRLGGEMKKNGVHIPIILFTYYNPVLQLDTDHFFALLRQNQLDGL
HHHHHHCCHHHHHHHHHHCCCHHHCCEEEEEEEEEECCCEEEECHHHHHHHHHHCCCCCE
LVPDLPFEESVYLQQQCKKHGITYISLVAPTSESRIQKITQQAEGFVYCVSSLGVTGVRN
ECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCEEEHHHCCCCCCHH
EFEDSVSSFIRSVKGMSSVPVAVGFGISSSKQVEMMNELADGVVVGSALVRKIEELKDEL
HHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAPETREQALKAFEAYAKTFSGSYMVK
CCCHHHHHHHHHHHHHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA