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The map label for this gene is trpB

Identifier: 52786143

GI number: 52786143

Start: 2342968

End: 2344194

Strand: Reverse

Name: trpB

Synonym: BLi02399

Alternate gene names: 52786143

Gene position: 2344194-2342968 (Counterclockwise)

Preceding gene: 52786144

Following gene: 52786142

Centisome position: 55.51

GC content: 50.37

Gene sequence:

>1227_bases
ATGTATTCATATCCGAATGAACAAGGCAGGTTTGGTGATTTTGGCGGGAAATTCGTCCCGGAAACACTCATGCAGCCTTT
GGAAGAAATCGAAAAAGCGTTTCTTGAACTGAAAGACGACCCTTCATTTCAAAAGGATTATCAAAAGCTTTTAAATGACT
ACTCAGGCAGGCCGACGGCTTTGACGTTCGCAGACCAGCTCACTAAGCAGCTGGGCGGAGCGAAGATTTACTTAAAAAGA
GAAGATTTAAACCATACAGGCGCCCACAAAATCAACAATGCCCTCGGCCAGGCGCTCCTGGCCAAGAAAATGGGAAAAAC
AAAGCTCATTGCTGAAACCGGTGCAGGCCAGCACGGTGTGGCGGCGGCGACTGTCGCCGCGAAGTTCGGCTTATCATGCA
TCGTCTTCATGGGGGAGGAAGATGTTGCCAGACAATCGCTGAACGTGTTCCGGATGAAGCTCTTGGGAGCGGAGGTCGTC
CCGGTGTCAAGCGGAAACGGGACATTAAAAGATGCTACAAACGAAGCGATCCGCTACTGGGTTCAGCATTGCCAGGACCA
TTTTTACATGATCGGTTCCGTCGTCGGGCCGCATCCGTATCCTTATATCGTCCGCGAATTTCAACGGATGATCGGCGAAG
AGGCGAAAGCGCAGTTTTATCAATTGGAGCAAAGCCTCCCCGATAAAGTTGTCGCCTGTGTCGGAGGCGGAAGCAATGCG
ATCGGGATGTTCAGCGCGTTCATTGAAGAAACTGTTGAACTGATCGGAGTCGAAGCTGCGGGAAAAGGTGTTGACACACC
TCTTCATGCGGCCACGATCACAAAGGGTACAAAAGGCGTCATCCACGGTTCTTTGACCTACTTGATTCAAGACGAGTACG
GACAGATCATCGAGCCTTATTCCATCTCAGCCGGACTTGATTATCCGGGAGTCGGCCCCGAGCACGCCCATCTTCATCAA
ACAGGGCGCGTCACGTATGAATCTGTCACAGATGATGAAGCGGTCTCGGCATTAAGGCTGCTGACAGAGACTGAGGGCAT
TTTGCCGGCTATTGAATCCGCCCATGCATTGGCAAAAGCATTTGAAATCGCTAAAGAACTGCCGAGAGAGAAAAGCGTTC
TCGTCTGCCTTTCAGGCAGGGGAGACAAAGACGTCCATACCTTGATGAAGGTGTTGGAAGAAGAGGTGAATCAAGATGTT
CAATCTGAAAAAACAGCCGTCCGATAA

Upstream 100 bases:

>100_bases
AAGCGGCGTCCGCCGGGAATCGACTTGGCAAGCGGAATCGAAGAAAGAGGCAGAAAAAGCGAAAAGCTGATCAGCCTTTT
AGAAGAAAGGATGTTCGAAC

Downstream 100 bases:

>100_bases
GCTATTTGTTCCGTTTATTACAGCGGGAGACCCGCTGCCAGAAATCTCGATCGAGCTGGCAAAATCTCTTCAAGAGGCGG
GAGCGTCCGCCCTTGAACTC

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MYSYPNEQGRFGDFGGKFVPETLMQPLEEIEKAFLELKDDPSFQKDYQKLLNDYSGRPTALTFADQLTKQLGGAKIYLKR
EDLNHTGAHKINNALGQALLAKKMGKTKLIAETGAGQHGVAAATVAAKFGLSCIVFMGEEDVARQSLNVFRMKLLGAEVV
PVSSGNGTLKDATNEAIRYWVQHCQDHFYMIGSVVGPHPYPYIVREFQRMIGEEAKAQFYQLEQSLPDKVVACVGGGSNA
IGMFSAFIEETVELIGVEAAGKGVDTPLHAATITKGTKGVIHGSLTYLIQDEYGQIIEPYSISAGLDYPGVGPEHAHLHQ
TGRVTYESVTDDEAVSALRLLTETEGILPAIESAHALAKAFEIAKELPREKSVLVCLSGRGDKDVHTLMKVLEEEVNQDV
QSEKTAVR

Sequences:

>Translated_408_residues
MYSYPNEQGRFGDFGGKFVPETLMQPLEEIEKAFLELKDDPSFQKDYQKLLNDYSGRPTALTFADQLTKQLGGAKIYLKR
EDLNHTGAHKINNALGQALLAKKMGKTKLIAETGAGQHGVAAATVAAKFGLSCIVFMGEEDVARQSLNVFRMKLLGAEVV
PVSSGNGTLKDATNEAIRYWVQHCQDHFYMIGSVVGPHPYPYIVREFQRMIGEEAKAQFYQLEQSLPDKVVACVGGGSNA
IGMFSAFIEETVELIGVEAAGKGVDTPLHAATITKGTKGVIHGSLTYLIQDEYGQIIEPYSISAGLDYPGVGPEHAHLHQ
TGRVTYESVTDDEAVSALRLLTETEGILPAIESAHALAKAFEIAKELPREKSVLVCLSGRGDKDVHTLMKVLEEEVNQDV
QSEKTAVR
>Mature_408_residues
MYSYPNEQGRFGDFGGKFVPETLMQPLEEIEKAFLELKDDPSFQKDYQKLLNDYSGRPTALTFADQLTKQLGGAKIYLKR
EDLNHTGAHKINNALGQALLAKKMGKTKLIAETGAGQHGVAAATVAAKFGLSCIVFMGEEDVARQSLNVFRMKLLGAEVV
PVSSGNGTLKDATNEAIRYWVQHCQDHFYMIGSVVGPHPYPYIVREFQRMIGEEAKAQFYQLEQSLPDKVVACVGGGSNA
IGMFSAFIEETVELIGVEAAGKGVDTPLHAATITKGTKGVIHGSLTYLIQDEYGQIIEPYSISAGLDYPGVGPEHAHLHQ
TGRVTYESVTDDEAVSALRLLTETEGILPAIESAHALAKAFEIAKELPREKSVLVCLSGRGDKDVHTLMKVLEEEVNQDV
QSEKTAVR

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=385, Percent_Identity=57.1428571428571, Blast_Score=442, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6321412, Length=385, Percent_Identity=56.3636363636364, Blast_Score=454, Evalue=1e-128,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB_BACLD (Q65I35)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_079563.1
- RefSeq:   YP_091972.1
- HSSP:   P0A2K1
- ProteinModelPortal:   Q65I35
- SMR:   Q65I35
- STRING:   Q65I35
- EnsemblBacteria:   EBBACT00000054445
- EnsemblBacteria:   EBBACT00000062327
- GeneID:   3028127
- GeneID:   3098719
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi02399
- KEGG:   bli:BL02771
- NMPDR:   fig|279010.5.peg.249
- eggNOG:   COG0133
- GeneTree:   EBGT00050000001631
- HOGENOM:   HBG303148
- OMA:   LKREDLC
- ProtClustDB:   PRK04346
- BioCyc:   BLIC279010-1:BLI02399-MONOMER
- BioCyc:   BLIC279010:BL02771-MONOMER
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026
- TIGRFAMs:   TIGR00263

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 44504; Mature: 44504

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSYPNEQGRFGDFGGKFVPETLMQPLEEIEKAFLELKDDPSFQKDYQKLLNDYSGRPTA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCE
LTFADQLTKQLGGAKIYLKREDLNHTGAHKINNALGQALLAKKMGKTKLIAETGAGQHGV
EHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCH
AAATVAAKFGLSCIVFMGEEDVARQSLNVFRMKLLGAEVVPVSSGNGTLKDATNEAIRYW
HHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHH
VQHCQDHFYMIGSVVGPHPYPYIVREFQRMIGEEAKAQFYQLEQSLPDKVVACVGGGSNA
HHHHHHHHEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCH
IGMFSAFIEETVELIGVEAAGKGVDTPLHAATITKGTKGVIHGSLTYLIQDEYGQIIEPY
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCEEECCEEEEEECCCCCEECCC
SISAGLDYPGVGPEHAHLHQTGRVTYESVTDDEAVSALRLLTETEGILPAIESAHALAKA
CCCCCCCCCCCCCCHHHHHHCCCEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
FEIAKELPREKSVLVCLSGRGDKDVHTLMKVLEEEVNQDVQSEKTAVR
HHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHCCC
>Mature Secondary Structure
MYSYPNEQGRFGDFGGKFVPETLMQPLEEIEKAFLELKDDPSFQKDYQKLLNDYSGRPTA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCE
LTFADQLTKQLGGAKIYLKREDLNHTGAHKINNALGQALLAKKMGKTKLIAETGAGQHGV
EHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCH
AAATVAAKFGLSCIVFMGEEDVARQSLNVFRMKLLGAEVVPVSSGNGTLKDATNEAIRYW
HHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHH
VQHCQDHFYMIGSVVGPHPYPYIVREFQRMIGEEAKAQFYQLEQSLPDKVVACVGGGSNA
HHHHHHHHEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCH
IGMFSAFIEETVELIGVEAAGKGVDTPLHAATITKGTKGVIHGSLTYLIQDEYGQIIEPY
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCEEECCEEEEEECCCCCEECCC
SISAGLDYPGVGPEHAHLHQTGRVTYESVTDDEAVSALRLLTETEGILPAIESAHALAKA
CCCCCCCCCCCCCCHHHHHHCCCEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
FEIAKELPREKSVLVCLSGRGDKDVHTLMKVLEEEVNQDVQSEKTAVR
HHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA