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The map label for this gene is yoaE [H]

Identifier: 52785923

GI number: 52785923

Start: 2133567

End: 2135606

Strand: Direct

Name: yoaE [H]

Synonym: BLi02174

Alternate gene names: 52785923

Gene position: 2133567-2135606 (Clockwise)

Preceding gene: 52785922

Following gene: 52785925

Centisome position: 50.53

GC content: 49.41

Gene sequence:

>2040_bases
ATGGAGTCATATGTTGATCAAAAAGACGGGATTTTCAAATCCGTATGTTCGCTCGACTGCCCGGATCAATGCGGGCTGCT
CGTTCATAAAAAAGACGGAAAAATCGTTAAAATACAGGGCGACCCGGACCATCCGGTCACACAGGGCAATATTTGCAATA
AAGTCCGCAATATGACGGAGCGTATTTATGACCCCAAACGGCTGAAAACCCCGCTGAAACGGACGGGTCCAAAAGGAGCC
GGGATGTTTGAACCGATCAGCTGGGATGAAGCGATTGAAACGATTCGCACCCGCTGGCAGTCGCTCATTGAACAGGAAGG
CCCCGAAAGCATTCTGCCCTACAGCTTTTACGGAAATATGGGAAACTTGACGGCTGAAGGAATGGACAGGCGCTTTTTCC
ACCGCCTCGGTTCCAGCCAGCTTGACCGGGCGATTTGCACCCCTGCCGGTTCTGTCGGCTATAAATACACGATGGGCGGA
AGCATCGGCACCGATCCTGAAGATACAACGGAGACAAAACTGTTTATTTTTTGGGGAATCAACGCGGTCTCAACCAACAT
GCATCAAATCACCATTGCTCAAAAAGCCCGGAAAAACGGCGCAAAAATCGTCGTAATCGATGTTCATAAAAATCAGACCG
GCCGGATGGCCGACTGGTTTATTCCGATTCGCCCCGGCACAGACAGCGCGCTTGCGCTTGGCATCATGCATGTTTTATTC
AAGGAACATTTGGCTGATGAATCATTTTTGAAAGAATTTACAGTCGGCCACGAAGAGCTGCGCCGCCACGTGCGGCAATA
CGACCCCGCCGCAGTAGAGGCGGTCACCGGCGTATCCCGGAATGATATTATCGAGCTTGCCCGCCTGTACGGCCGCACCT
CCCCTTCATTGATTCGGATCGGCAACGGTCTTCAGCATCATGATAATGGAGGAATGATCGTTCGCACCATCTCGTGCCTG
CCCGCGATCACGGGCCAATGGACGAAAAAAGGAGGCGGAGCGATCAAAGGCAACACGGCATTTTTGGCCTATAATACAAA
AGCATTGCAGCGCCCCGATTTATTAAAAGGCAGAACGCCGAGAGTCATCAACATGAATCAGCTCGGCAAAGCTCTCTTAG
AAACCGAACCACCGATCCGTTCGCTGTTTGTGTACAGCAGCAATCCGGCCGTTGTCGCGCCTGAGGCGAACAAAGTCAGA
AAAGGACTTGAGCGCGAAGATTTATTCACGGTTGTTCACGATCTATTTCTGACGGAAACGGCACGGTACGCCGATATCGT
CCTGCCGGCTTCTTCCGCTTTCGAAAATACGGATTTCTATACATCATATTGGCACCATTATGCGCAGATTCAGAAGCCTG
TGATTGAGAAATACGGCGAGAGCAAGTCAAATACAGAAGTATTCAGGCTTTTGGCTGAGGCGATGGGCTTTGATGATGAG
CCGCTGAAAGATTCCGACGAAGAGATGATCAGACAGGCGCTTGACTACCCTGATAATCCGTGGCTCGATGGCATTGACTA
TGACAGCCTGACTGAAAAGCATTTTGTCAAAGCAAATCGAAAAACGGTTTTTCCTGATAAACTGCCGACGCCTAGCGGAA
AAATTGAATTGTATTCAAAAATGATGGAGCAGCACGGCTATCCGGGCCTGCCGACTCATACCCCGCTCATTGAAACGGAC
AAGTATCCATTTTTATTTGTGCCCGGCCCGAATCACAATTTTTTAAATTCGACCTTTTCAAACAATGAAAAACATATCAA
ATTAGAAAAGGAGCCGAAACTTCACTTAAATATAAAAGATGCCGAAGAAAAGGGCATCGAAAATGGAGATTTGGTGAAAG
TTTGGAATGACCGCGGCTCATGCATCCTGCCCGCTGCCGTCGGAGAAAACGTCCTTCCGGGCGTGGCCGTCAGCCAGGGA
TTGTGGGCGGATGCAGAAGGGAAGCATTATCTCGTCAATGCGCTGACGCCCGACCGTTTAGCTGATATGGGCGGTGGTGC
AACTTTCTTTTCAGGAAGAGTTCAAATTGAAAAAATATGA

Upstream 100 bases:

>100_bases
TTTTTCTGCCGGGATTCTTCCGCTCTTATTTTCAGAATTGACGACATTTTGTGATATAATGCTAGAATCATATTCATTTT
ATCGGAAAGGAACGGATTCC

Downstream 100 bases:

>100_bases
ACATCAAAAAAGACCGGTTGCAGCAAAACCGGTCTTTTATCACTTCGTCTTTTTTAAGGTTTTGGCATAATCGGCGGCAA
ACGCCGTGCTGTTTTTCAAA

Product: YoaE

Products: Reduced form of N-Oxide compounds [C]

Alternate protein names: NA

Number of amino acids: Translated: 679; Mature: 679

Protein sequence:

>679_residues
MESYVDQKDGIFKSVCSLDCPDQCGLLVHKKDGKIVKIQGDPDHPVTQGNICNKVRNMTERIYDPKRLKTPLKRTGPKGA
GMFEPISWDEAIETIRTRWQSLIEQEGPESILPYSFYGNMGNLTAEGMDRRFFHRLGSSQLDRAICTPAGSVGYKYTMGG
SIGTDPEDTTETKLFIFWGINAVSTNMHQITIAQKARKNGAKIVVIDVHKNQTGRMADWFIPIRPGTDSALALGIMHVLF
KEHLADESFLKEFTVGHEELRRHVRQYDPAAVEAVTGVSRNDIIELARLYGRTSPSLIRIGNGLQHHDNGGMIVRTISCL
PAITGQWTKKGGGAIKGNTAFLAYNTKALQRPDLLKGRTPRVINMNQLGKALLETEPPIRSLFVYSSNPAVVAPEANKVR
KGLEREDLFTVVHDLFLTETARYADIVLPASSAFENTDFYTSYWHHYAQIQKPVIEKYGESKSNTEVFRLLAEAMGFDDE
PLKDSDEEMIRQALDYPDNPWLDGIDYDSLTEKHFVKANRKTVFPDKLPTPSGKIELYSKMMEQHGYPGLPTHTPLIETD
KYPFLFVPGPNHNFLNSTFSNNEKHIKLEKEPKLHLNIKDAEEKGIENGDLVKVWNDRGSCILPAAVGENVLPGVAVSQG
LWADAEGKHYLVNALTPDRLADMGGGATFFSGRVQIEKI

Sequences:

>Translated_679_residues
MESYVDQKDGIFKSVCSLDCPDQCGLLVHKKDGKIVKIQGDPDHPVTQGNICNKVRNMTERIYDPKRLKTPLKRTGPKGA
GMFEPISWDEAIETIRTRWQSLIEQEGPESILPYSFYGNMGNLTAEGMDRRFFHRLGSSQLDRAICTPAGSVGYKYTMGG
SIGTDPEDTTETKLFIFWGINAVSTNMHQITIAQKARKNGAKIVVIDVHKNQTGRMADWFIPIRPGTDSALALGIMHVLF
KEHLADESFLKEFTVGHEELRRHVRQYDPAAVEAVTGVSRNDIIELARLYGRTSPSLIRIGNGLQHHDNGGMIVRTISCL
PAITGQWTKKGGGAIKGNTAFLAYNTKALQRPDLLKGRTPRVINMNQLGKALLETEPPIRSLFVYSSNPAVVAPEANKVR
KGLEREDLFTVVHDLFLTETARYADIVLPASSAFENTDFYTSYWHHYAQIQKPVIEKYGESKSNTEVFRLLAEAMGFDDE
PLKDSDEEMIRQALDYPDNPWLDGIDYDSLTEKHFVKANRKTVFPDKLPTPSGKIELYSKMMEQHGYPGLPTHTPLIETD
KYPFLFVPGPNHNFLNSTFSNNEKHIKLEKEPKLHLNIKDAEEKGIENGDLVKVWNDRGSCILPAAVGENVLPGVAVSQG
LWADAEGKHYLVNALTPDRLADMGGGATFFSGRVQIEKI
>Mature_679_residues
MESYVDQKDGIFKSVCSLDCPDQCGLLVHKKDGKIVKIQGDPDHPVTQGNICNKVRNMTERIYDPKRLKTPLKRTGPKGA
GMFEPISWDEAIETIRTRWQSLIEQEGPESILPYSFYGNMGNLTAEGMDRRFFHRLGSSQLDRAICTPAGSVGYKYTMGG
SIGTDPEDTTETKLFIFWGINAVSTNMHQITIAQKARKNGAKIVVIDVHKNQTGRMADWFIPIRPGTDSALALGIMHVLF
KEHLADESFLKEFTVGHEELRRHVRQYDPAAVEAVTGVSRNDIIELARLYGRTSPSLIRIGNGLQHHDNGGMIVRTISCL
PAITGQWTKKGGGAIKGNTAFLAYNTKALQRPDLLKGRTPRVINMNQLGKALLETEPPIRSLFVYSSNPAVVAPEANKVR
KGLEREDLFTVVHDLFLTETARYADIVLPASSAFENTDFYTSYWHHYAQIQKPVIEKYGESKSNTEVFRLLAEAMGFDDE
PLKDSDEEMIRQALDYPDNPWLDGIDYDSLTEKHFVKANRKTVFPDKLPTPSGKIELYSKMMEQHGYPGLPTHTPLIETD
KYPFLFVPGPNHNFLNSTFSNNEKHIKLEKEPKLHLNIKDAEEKGIENGDLVKVWNDRGSCILPAAVGENVLPGVAVSQG
LWADAEGKHYLVNALTPDRLADMGGGATFFSGRVQIEKI

Specific function: Terminal Reductase During Anaerobic Growth On Various Sulfoxide And N-Oxide Compounds. Allows E.Coli To Grow Anaerobically On Me(2)So As Respiratory Oxidant. [C]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081797, Length=766, Percent_Identity=29.7650130548303, Blast_Score=255, Evalue=5e-69,
Organism=Escherichia coli, GI1787870, Length=765, Percent_Identity=29.9346405228758, Blast_Score=235, Evalue=7e-63,
Organism=Escherichia coli, GI171474008, Length=761, Percent_Identity=28.2522996057819, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI3868721, Length=692, Percent_Identity=26.7341040462428, Blast_Score=189, Evalue=6e-49,
Organism=Escherichia coli, GI87081994, Length=763, Percent_Identity=25.5570117955439, Blast_Score=152, Evalue=7e-38,
Organism=Escherichia coli, GI145693196, Length=719, Percent_Identity=25.0347705146036, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1787231, Length=712, Percent_Identity=23.876404494382, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1788534, Length=552, Percent_Identity=21.5579710144928, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI3868720, Length=450, Percent_Identity=23.5555555555556, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI3868719, Length=435, Percent_Identity=22.5287356321839, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1787778, Length=298, Percent_Identity=26.8456375838926, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 75913; Mature: 75913

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00490 MOLYBDOPTERIN_PROK_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESYVDQKDGIFKSVCSLDCPDQCGLLVHKKDGKIVKIQGDPDHPVTQGNICNKVRNMTE
CCCCCCCCCCHHHHHHCCCCCCCCCEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHH
RIYDPKRLKTPLKRTGPKGAGMFEPISWDEAIETIRTRWQSLIEQEGPESILPYSFYGNM
HHCCCHHHCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCC
GNLTAEGMDRRFFHRLGSSQLDRAICTPAGSVGYKYTMGGSIGTDPEDTTETKLFIFWGI
CCCCHHHHHHHHHHHHCHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCEEEEEEECC
NAVSTNMHQITIAQKARKNGAKIVVIDVHKNQTGRMADWFIPIRPGTDSALALGIMHVLF
CHHCCCCEEEEEHHHHHHCCCEEEEEEECCCCCCCEEEEEEEECCCCCHHHHHHHHHHHH
KEHLADESFLKEFTVGHEELRRHVRQYDPAAVEAVTGVSRNDIIELARLYGRTSPSLIRI
HHHHCCHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEE
GNGLQHHDNGGMIVRTISCLPAITGQWTKKGGGAIKGNTAFLAYNTKALQRPDLLKGRTP
CCCCEECCCCCEEEEEEHHHHHHCCCCCCCCCCEEECCEEEEEECCHHHCCCHHHCCCCC
RVINMNQLGKALLETEPPIRSLFVYSSNPAVVAPEANKVRKGLEREDLFTVVHDLFLTET
CEEEHHHHHHHHHCCCCCCEEEEEECCCCCEECCCHHHHHCCCCHHHHHHHHHHHHHHCC
ARYADIVLPASSAFENTDFYTSYWHHYAQIQKPVIEKYGESKSNTEVFRLLAEAMGFDDE
CCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
PLKDSDEEMIRQALDYPDNPWLDGIDYDSLTEKHFVKANRKTVFPDKLPTPSGKIELYSK
CCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHH
MMEQHGYPGLPTHTPLIETDKYPFLFVPGPNHNFLNSTFSNNEKHIKLEKEPKLHLNIKD
HHHHCCCCCCCCCCCCEECCCCCEEEECCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEC
AEEKGIENGDLVKVWNDRGSCILPAAVGENVLPGVAVSQGLWADAEGKHYLVNALTPDRL
HHHCCCCCCCEEEEECCCCCEEEEHHCCCCCCCCCHHCCCCCCCCCCCEEEEECCCHHHH
ADMGGGATFFSGRVQIEKI
HHCCCCCEEEECEEEEEEC
>Mature Secondary Structure
MESYVDQKDGIFKSVCSLDCPDQCGLLVHKKDGKIVKIQGDPDHPVTQGNICNKVRNMTE
CCCCCCCCCCHHHHHHCCCCCCCCCEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHH
RIYDPKRLKTPLKRTGPKGAGMFEPISWDEAIETIRTRWQSLIEQEGPESILPYSFYGNM
HHCCCHHHCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCC
GNLTAEGMDRRFFHRLGSSQLDRAICTPAGSVGYKYTMGGSIGTDPEDTTETKLFIFWGI
CCCCHHHHHHHHHHHHCHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCEEEEEEECC
NAVSTNMHQITIAQKARKNGAKIVVIDVHKNQTGRMADWFIPIRPGTDSALALGIMHVLF
CHHCCCCEEEEEHHHHHHCCCEEEEEEECCCCCCCEEEEEEEECCCCCHHHHHHHHHHHH
KEHLADESFLKEFTVGHEELRRHVRQYDPAAVEAVTGVSRNDIIELARLYGRTSPSLIRI
HHHHCCHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEE
GNGLQHHDNGGMIVRTISCLPAITGQWTKKGGGAIKGNTAFLAYNTKALQRPDLLKGRTP
CCCCEECCCCCEEEEEEHHHHHHCCCCCCCCCCEEECCEEEEEECCHHHCCCHHHCCCCC
RVINMNQLGKALLETEPPIRSLFVYSSNPAVVAPEANKVRKGLEREDLFTVVHDLFLTET
CEEEHHHHHHHHHCCCCCCEEEEEECCCCCEECCCHHHHHCCCCHHHHHHHHHHHHHHCC
ARYADIVLPASSAFENTDFYTSYWHHYAQIQKPVIEKYGESKSNTEVFRLLAEAMGFDDE
CCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
PLKDSDEEMIRQALDYPDNPWLDGIDYDSLTEKHFVKANRKTVFPDKLPTPSGKIELYSK
CCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHH
MMEQHGYPGLPTHTPLIETDKYPFLFVPGPNHNFLNSTFSNNEKHIKLEKEPKLHLNIKD
HHHHCCCCCCCCCCCCEECCCCCEEEECCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEC
AEEKGIENGDLVKVWNDRGSCILPAAVGENVLPGVAVSQGLWADAEGKHYLVNALTPDRL
HHHCCCCCCCEEEEECCCCCEEEEHHCCCCCCCCCHHCCCCCCCCCCCEEEEECCCHHHH
ADMGGGATFFSGRVQIEKI
HHCCCCCEEEECEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: Fe; Mo [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Oxide; Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

Specific reaction: Reduces Various N-Oxide And Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]