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The map label for this gene is lmrB [H]

Identifier: 52785922

GI number: 52785922

Start: 2131995

End: 2133419

Strand: Direct

Name: lmrB [H]

Synonym: BLi02173

Alternate gene names: 52785922

Gene position: 2131995-2133419 (Clockwise)

Preceding gene: 52785921

Following gene: 52785923

Centisome position: 50.49

GC content: 47.44

Gene sequence:

>1425_bases
ATGGAGAAAAATTTAAATTCCAAAGCAATATTAGTCACCTTCATGATTGGTGCATTTTTTGCAATATTAAACGAAACGCT
GTTGAACATAGCATTAACAGAGCTGATGGAAGTCTTTCACGTTGATGCCCCGACCGTTCAATGGCTGGCAACCGGATTTA
TGCTTGTAATGGGTGTGTTAATGCCTATTTCCGCTTTACTGATTCAATGGTTCACGACAAGACAGATGTTTATCGGCGTT
ATGTCGATTTTTTTAGCAGGAACCGTCATTGCCGCTTGTGCAGTGAATTTCCCGATGCTCTTGACGGGGCGCATGATTCA
GGCAGTCGGTACGGGACTCTTGATCCCCATTATTATGAACGCCCTCTTGCTGATTTACCGGCCTGAAGTCCGCGGGAAAA
TCATGGGGACGTTCGGACTGGTCATCATGTTTGCTCCGGCGATCGGACCCACTTTTTCGGGCGTTATTGTTGACATCCTC
GGCTGGCGCTGGCTGTTTATCATCGTTATTCCTTTTGCAGTATTTTCAATTCTGTTTGCGGTAAAGTATTTGCAAAATGT
CGGAGAACCGACGCGCCCGCGGGCTGATTTTTTATCGATCATACTCTCAACCATCGGGATCGGCGGAATCGTATACGGAT
TCAGCAGTGCCGGAGAGGCTCAGGAAGGGTTTCAGTCCCCTCTGATCATCGGTGTGATCATCATCAGCCTGATCAGCATC
GTGTTGTTCGTGCTTCGGCAGTTGAAACTTGAACAACCGTTGCTTGATGTGCGTGTGTTCGCCTATAAAAATTACACGCG
CGGGGTCTGCCTTTTTGTGATTGTCATTATGGCGATGTTCGCTTCCGAAATTGTGATGCCGATGTATTTGCAAGGGCCGC
TCGGTTTTTCAGCAAAAGCAGCAGGTCTTTTGCTTTTGCCCGGAGCCTTGCTAAACGGTCTCATGTCGCCGGCTATGGGC
GCTTTGTTCGATAAGTTCGGCCCGAGAAAACTCATCATTCCAGGTACGATCGTTCTCGTCGGCGTGATGTTTTTCTTCAG
CAGGATCAATCCTTCGGTGCCGATTTGGGCGTTCATTATTGTATACATCATCTTGATGCTGTCCATCTCGGCCATCATGA
TGCCCGCACAGACGAATGCATTAAACGAATTGCCGAAGCATTTATATCCTCATGGAACGGCAATTTCCAATACTCTTCAG
CCTGTTGCAGGCGCACTCGGTGTTTCGGTGTTCGTCAGCATCATGAGCCAGGGACAGAAAAGCTATCTTGCCGGCCAAGA
AGCAGCCAGCAAACAAATCGTGCATGAAGCGATGACTCATGGTGTTCACCATGCCTACTGGTTTGCGCTCGCTTTAAGCT
GTATCGCTTTCTTTACCGCTTTATTTATTAAAAAAGCGGTCGCGCCTGATTTTGATAAAGAATAA

Upstream 100 bases:

>100_bases
ATGAAAAACGAAAAAAGCAAATTAGGACCCCGGAGATTCGGAGTCCTACATATCATTTGTTGTCATGTTTTTGGGTAAAT
TGTATTACAGGAGGTTTGAT

Downstream 100 bases:

>100_bases
CCAAAAACTTAAAGGATGTCCTTCCTCGGGGCATCCTTTTTTATCATTTTTTCTGCCGGGATTCTTCCGCTCTTATTTTC
AGAATTGACGACATTTTGTG

Product: LmrB

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MEKNLNSKAILVTFMIGAFFAILNETLLNIALTELMEVFHVDAPTVQWLATGFMLVMGVLMPISALLIQWFTTRQMFIGV
MSIFLAGTVIAACAVNFPMLLTGRMIQAVGTGLLIPIIMNALLLIYRPEVRGKIMGTFGLVIMFAPAIGPTFSGVIVDIL
GWRWLFIIVIPFAVFSILFAVKYLQNVGEPTRPRADFLSIILSTIGIGGIVYGFSSAGEAQEGFQSPLIIGVIIISLISI
VLFVLRQLKLEQPLLDVRVFAYKNYTRGVCLFVIVIMAMFASEIVMPMYLQGPLGFSAKAAGLLLLPGALLNGLMSPAMG
ALFDKFGPRKLIIPGTIVLVGVMFFFSRINPSVPIWAFIIVYIILMLSISAIMMPAQTNALNELPKHLYPHGTAISNTLQ
PVAGALGVSVFVSIMSQGQKSYLAGQEAASKQIVHEAMTHGVHHAYWFALALSCIAFFTALFIKKAVAPDFDKE

Sequences:

>Translated_474_residues
MEKNLNSKAILVTFMIGAFFAILNETLLNIALTELMEVFHVDAPTVQWLATGFMLVMGVLMPISALLIQWFTTRQMFIGV
MSIFLAGTVIAACAVNFPMLLTGRMIQAVGTGLLIPIIMNALLLIYRPEVRGKIMGTFGLVIMFAPAIGPTFSGVIVDIL
GWRWLFIIVIPFAVFSILFAVKYLQNVGEPTRPRADFLSIILSTIGIGGIVYGFSSAGEAQEGFQSPLIIGVIIISLISI
VLFVLRQLKLEQPLLDVRVFAYKNYTRGVCLFVIVIMAMFASEIVMPMYLQGPLGFSAKAAGLLLLPGALLNGLMSPAMG
ALFDKFGPRKLIIPGTIVLVGVMFFFSRINPSVPIWAFIIVYIILMLSISAIMMPAQTNALNELPKHLYPHGTAISNTLQ
PVAGALGVSVFVSIMSQGQKSYLAGQEAASKQIVHEAMTHGVHHAYWFALALSCIAFFTALFIKKAVAPDFDKE
>Mature_474_residues
MEKNLNSKAILVTFMIGAFFAILNETLLNIALTELMEVFHVDAPTVQWLATGFMLVMGVLMPISALLIQWFTTRQMFIGV
MSIFLAGTVIAACAVNFPMLLTGRMIQAVGTGLLIPIIMNALLLIYRPEVRGKIMGTFGLVIMFAPAIGPTFSGVIVDIL
GWRWLFIIVIPFAVFSILFAVKYLQNVGEPTRPRADFLSIILSTIGIGGIVYGFSSAGEAQEGFQSPLIIGVIIISLISI
VLFVLRQLKLEQPLLDVRVFAYKNYTRGVCLFVIVIMAMFASEIVMPMYLQGPLGFSAKAAGLLLLPGALLNGLMSPAMG
ALFDKFGPRKLIIPGTIVLVGVMFFFSRINPSVPIWAFIIVYIILMLSISAIMMPAQTNALNELPKHLYPHGTAISNTLQ
PVAGALGVSVFVSIMSQGQKSYLAGQEAASKQIVHEAMTHGVHHAYWFALALSCIAFFTALFIKKAVAPDFDKE

Specific function: Proton-dependent transporter. May mediate the efflux of lincomycin [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1788710, Length=427, Percent_Identity=22.0140515222482, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1790195, Length=403, Percent_Identity=26.0545905707196, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1789042, Length=423, Percent_Identity=25.7683215130024, Blast_Score=125, Evalue=7e-30,
Organism=Escherichia coli, GI87081983, Length=316, Percent_Identity=26.2658227848101, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1788392, Length=448, Percent_Identity=21.875, Blast_Score=75, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 51635; Mature: 51635

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure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HHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274031; 9384377 [H]