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The map label for this gene is mdr [H]

Identifier: 52785925

GI number: 52785925

Start: 2136370

End: 2137782

Strand: Direct

Name: mdr [H]

Synonym: BLi02176

Alternate gene names: 52785925

Gene position: 2136370-2137782 (Clockwise)

Preceding gene: 52785923

Following gene: 52785932

Centisome position: 50.59

GC content: 47.84

Gene sequence:

>1413_bases
TTGTCGGCGATCTTGGCGGACTCGACAGCTTTGTCTGGGTTGTATCTGCCTTTATGGTCGCTGAAATGGCCGGTATGCCG
ATATTCGGCAAGCTGTCGGATATGTATGGCAGAAAACGTTTTTTTTCTTTTTGGCAGCATCGTTTTCATGGCGGGATCAG
CATTGTGCGGAACGGCTACAACCATTGAGCAGCTCAGCATATACCGCGCGATCCAGGGAATTGGCGGAGGCGCGCTCATT
CCGATTGCTTTTACGATTATCTTTGACATTTTTCCGCTGGAAAAGCGCGGTAAGATCACTGGACTTTTCGGTGCTGTTTT
TGGCCTTTCAAGCATTTTCGGACCGCTCATCGGCGCCTATGTCACTGACTACATCAGCTGGCACTGGATTTTTTATATGA
ATCTGCCGCTCGGTATGGTTTCTTTTATTTTCATTCTTCTATTTTACAAAGAGACGAAACAGCATGAAAAGCATCGTATT
GATTGGCTCGGAGCGATTACGCTCGTAGGGGCGGTCGTATGCCTCATGTTTGCGTTGGAGCTCGGCGGCGGGAAATACGC
CTGGGATTCGAATGTCATCCTTACCCTTTTCGGAAGTGCAGCCGTCTTGTTGGTTTGCTTCCTCATGATCGAGCGCAAAG
CCAGTGAACCGATCATTTCGTTTCATATGTTTAAAAACCGGCTGTTTGCCGGCAGTACGCTTGTCGCCTTATTTTATGGA
GGGACATTTATTTCAGCGACCATCCAGATTCCAATCTTTGTTCAGGGTGTTTACGGAGATTCGGCGACAAACTCGGGACT
TGTGCTTTTGCCGATGATGCTCGGCTCTGTCGTATCAGCACAGCTTGGCGGATTCTTAACAACAAAAATGAGCTATCGGA
ACATTATGCTCATCTCTGGATTGGTTCTCGTCAGCGGATTTTCTTTATTAAGCACGCTTAACATTGACACTCCGCGTTTA
TTATTAACCATGTATATGATCGTCGTCGGCTTGGGAGTCGGTTTTTCTTTCTCTGTATTAAGCATGGCAGCGATCCATCA
TTTCGGCCCGGAGCAGCGCGGCTCAGCGACATCTACGAGCAACTTTATGCGCTCTCTCGGCATGACGATCGGCGCCAGTA
TGTTCGGGATGATTCAAAAAAACCAATTTTCAGAACAGCTGGAAGATATGTTTAAAAGCAGCGGCGCTTCAAATTTCGCC
TCAACGAATGACGCCGACCAGATTTTATCAAGTGCACGGGCATCACTGCCGCCGGATGTTTTACATAACATCACCGAAGC
GCTATCAAATTCGATTGTTCACACGTTCATTTGGGCGATTTCCCCAGCAGCGGCCGCCTTTTTACTGGTGTTCTTCATGC
CGAATGACAGGGTGAAAGTGAACGCAAAAAAGGAAGTGAACAAAGCCGGCTGA

Upstream 100 bases:

>100_bases
ACTCAAAATCAAAAATTAGGATTTGTTGTAGCAGGACTTTTGCTTGCCATTCTGATGGCTTCGATGGACAATACCATCGT
TGTTACGGCAATGGGAACAA

Downstream 100 bases:

>100_bases
ACCTCTAGCTTTGCACTGCCTTCTTTTTTCTCATTTTCGAAACCAATAAAAGCGGAATCGGCACAATGCCGAATAAAACG
GCCGCAATATAGCCGATCAT

Product: multidrug resistance protein B

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter 3 [H]

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MSAILADSTALSGLYLPLWSLKWPVCRYSASCRICMAENVFFLFGSIVFMAGSALCGTATTIEQLSIYRAIQGIGGGALI
PIAFTIIFDIFPLEKRGKITGLFGAVFGLSSIFGPLIGAYVTDYISWHWIFYMNLPLGMVSFIFILLFYKETKQHEKHRI
DWLGAITLVGAVVCLMFALELGGGKYAWDSNVILTLFGSAAVLLVCFLMIERKASEPIISFHMFKNRLFAGSTLVALFYG
GTFISATIQIPIFVQGVYGDSATNSGLVLLPMMLGSVVSAQLGGFLTTKMSYRNIMLISGLVLVSGFSLLSTLNIDTPRL
LLTMYMIVVGLGVGFSFSVLSMAAIHHFGPEQRGSATSTSNFMRSLGMTIGASMFGMIQKNQFSEQLEDMFKSSGASNFA
STNDADQILSSARASLPPDVLHNITEALSNSIVHTFIWAISPAAAAFLLVFFMPNDRVKVNAKKEVNKAG

Sequences:

>Translated_470_residues
MSAILADSTALSGLYLPLWSLKWPVCRYSASCRICMAENVFFLFGSIVFMAGSALCGTATTIEQLSIYRAIQGIGGGALI
PIAFTIIFDIFPLEKRGKITGLFGAVFGLSSIFGPLIGAYVTDYISWHWIFYMNLPLGMVSFIFILLFYKETKQHEKHRI
DWLGAITLVGAVVCLMFALELGGGKYAWDSNVILTLFGSAAVLLVCFLMIERKASEPIISFHMFKNRLFAGSTLVALFYG
GTFISATIQIPIFVQGVYGDSATNSGLVLLPMMLGSVVSAQLGGFLTTKMSYRNIMLISGLVLVSGFSLLSTLNIDTPRL
LLTMYMIVVGLGVGFSFSVLSMAAIHHFGPEQRGSATSTSNFMRSLGMTIGASMFGMIQKNQFSEQLEDMFKSSGASNFA
STNDADQILSSARASLPPDVLHNITEALSNSIVHTFIWAISPAAAAFLLVFFMPNDRVKVNAKKEVNKAG
>Mature_469_residues
SAILADSTALSGLYLPLWSLKWPVCRYSASCRICMAENVFFLFGSIVFMAGSALCGTATTIEQLSIYRAIQGIGGGALIP
IAFTIIFDIFPLEKRGKITGLFGAVFGLSSIFGPLIGAYVTDYISWHWIFYMNLPLGMVSFIFILLFYKETKQHEKHRID
WLGAITLVGAVVCLMFALELGGGKYAWDSNVILTLFGSAAVLLVCFLMIERKASEPIISFHMFKNRLFAGSTLVALFYGG
TFISATIQIPIFVQGVYGDSATNSGLVLLPMMLGSVVSAQLGGFLTTKMSYRNIMLISGLVLVSGFSLLSTLNIDTPRLL
LTMYMIVVGLGVGFSFSVLSMAAIHHFGPEQRGSATSTSNFMRSLGMTIGASMFGMIQKNQFSEQLEDMFKSSGASNFAS
TNDADQILSSARASLPPDVLHNITEALSNSIVHTFIWAISPAAAAFLLVFFMPNDRVKVNAKKEVNKAG

Specific function: Confers resistance to puromycin, tosufloxacin and norfloxacin [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=367, Percent_Identity=27.5204359673025, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1790195, Length=402, Percent_Identity=23.134328358209, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI87081983, Length=345, Percent_Identity=25.5072463768116, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1788710, Length=405, Percent_Identity=24.9382716049383, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1788392, Length=351, Percent_Identity=25.9259259259259, Blast_Score=90, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6322958, Length=471, Percent_Identity=24.203821656051, Blast_Score=113, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6319783, Length=456, Percent_Identity=23.6842105263158, Blast_Score=103, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6319770, Length=372, Percent_Identity=26.0752688172043, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6325455, Length=472, Percent_Identity=20.5508474576271, Blast_Score=83, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6321663, Length=485, Percent_Identity=24.1237113402062, Blast_Score=77, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6323735, Length=456, Percent_Identity=22.5877192982456, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50982; Mature: 50850

Theoretical pI: Translated: 8.55; Mature: 8.55

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAILADSTALSGLYLPLWSLKWPVCRYSASCRICMAENVFFLFGSIVFMAGSALCGTAT
CCCEECCCHHHCCHHHHHHHCCCCHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCHH
TIEQLSIYRAIQGIGGGALIPIAFTIIFDIFPLEKRGKITGLFGAVFGLSSIFGPLIGAY
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VTDYISWHWIFYMNLPLGMVSFIFILLFYKETKQHEKHRIDWLGAITLVGAVVCLMFALE
HHHHHHEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGGGKYAWDSNVILTLFGSAAVLLVCFLMIERKASEPIISFHMFKNRLFAGSTLVALFYG
CCCCCEEECCCEEEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHC
GTFISATIQIPIFVQGVYGDSATNSGLVLLPMMLGSVVSAQLGGFLTTKMSYRNIMLISG
CEEEEEEEEEEEEEEEEECCCCCCCCEEEHHHHHHHHHHHHHCCHHEEHHHHHHHHHHHH
LVLVSGFSLLSTLNIDTPRLLLTMYMIVVGLGVGFSFSVLSMAAIHHFGPEQRGSATSTS
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHH
NFMRSLGMTIGASMFGMIQKNQFSEQLEDMFKSSGASNFASTNDADQILSSARASLPPDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCHHH
LHNITEALSNSIVHTFIWAISPAAAAFLLVFFMPNDRVKVNAKKEVNKAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEECHHHCCCCC
>Mature Secondary Structure 
SAILADSTALSGLYLPLWSLKWPVCRYSASCRICMAENVFFLFGSIVFMAGSALCGTAT
CCEECCCHHHCCHHHHHHHCCCCHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCHH
TIEQLSIYRAIQGIGGGALIPIAFTIIFDIFPLEKRGKITGLFGAVFGLSSIFGPLIGAY
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VTDYISWHWIFYMNLPLGMVSFIFILLFYKETKQHEKHRIDWLGAITLVGAVVCLMFALE
HHHHHHEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGGGKYAWDSNVILTLFGSAAVLLVCFLMIERKASEPIISFHMFKNRLFAGSTLVALFYG
CCCCCEEECCCEEEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHC
GTFISATIQIPIFVQGVYGDSATNSGLVLLPMMLGSVVSAQLGGFLTTKMSYRNIMLISG
CEEEEEEEEEEEEEEEEECCCCCCCCEEEHHHHHHHHHHHHHCCHHEEHHHHHHHHHHHH
LVLVSGFSLLSTLNIDTPRLLLTMYMIVVGLGVGFSFSVLSMAAIHHFGPEQRGSATSTS
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHH
NFMRSLGMTIGASMFGMIQKNQFSEQLEDMFKSSGASNFASTNDADQILSSARASLPPDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCHHH
LHNITEALSNSIVHTFIWAISPAAAAFLLVFFMPNDRVKVNAKKEVNKAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEECHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023234; 9384377 [H]