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The map label for this gene is ykoW [H]

Identifier: 52785261

GI number: 52785261

Start: 1468790

End: 1470442

Strand: Reverse

Name: ykoW [H]

Synonym: BLi01496

Alternate gene names: 52785261

Gene position: 1470442-1468790 (Counterclockwise)

Preceding gene: 52785266

Following gene: 52785260

Centisome position: 34.82

GC content: 47.67

Gene sequence:

>1653_bases
GTGACAGAAGTTCTTGGTGAATATAATGGATGGCTCGTCCTATTATCCTATTTCACTGCAGTTTTGGCAGCTTATACAGC
CCTTGATTTATCTGAACGGGTCGTACATACAAAAGGCAGGAAAAATATCGGATGGCTTTTGGCCGGTTCCCTCACGATGG
GGCTTGGCATCTGGTCTATGCACTTTATCGGGATGGTGTCATTTCAGACAGCAGAAGAAATGAAATACGATTTGAAATTC
GTCTTGTTGTCGATTGTTGCCGCTTTCGCAGGCTCGCTCATCTCATTTTATGTGGTGAATGAAAAAAAACCGACGAATGG
AAGACTGGCGGCCGGGTCCCTTGCAATGGGATGCGCAATTGCCTCCATGCATTTTATCGGGATGGAAGCAATGGAAAATA
TGACAATCGTCTATCATCCCGTTCTTTATGCCGCCTCTTTTGCAATCGCCATTTTTGCCTCCTTCGCTGCCTTAAAACTG
TCAGTTGTATTCGCCAAAAAAACCGGTTCTTTTCGGATATTGTTCATTAAAATCGGAAGCGCGCTTTTAATGGGCGGAGC
GATTTCCGGCATGCACTATACAGGAATGTCAGCAGCTACGCTTTTCATGGCTGACAGCGTTGACACAGGCTATGAAGGAA
TCGATACCGTTGAACTGGGCATTATGATCGTGCTTGTCAGCCTGATGCTTCAATGCTTCTTGATTTTCGGCGCCTTCAAT
GACAAACGCCTGCTGCTTCATATCGAAAAAGTCAAAGATAACGAGGAGCGGTTCCAGTCCTTGATCAACCACAACATTGA
TCCGATCTATGTTTTTTCGCTTGAAGGCAAGCTGCTTTCCGCCAATTCATCGGGATACAGCCTTCTTGAAAAAATGGGAA
TTGAACCCGAGCGCCTGTCTTCCGTTTTTCGGCAGGAGGATCATGACAGGCTGCTCGGCTGGTTCCTTCAAGTCCAAAAT
GAAAAACAGGCCATCAACCGCGATACGGCAATCGCGATAGCTGAAAAGCGCTTTGATTTAAATGTGACGCTGATACCGGT
GTATGTCAGAAAGCAGCTTGACAGCATTTATGTCATATGCAAAGATATGACAAACCAGCGTGAAGCAGAACGCAAAATCC
ATAGAATGGCCCACTATGACGCGTTGACGGATTTGCCAAACAGAAGGTATGCAGTCGACCGTCTGGCAAATGTTCTAAAG
CGGCAGGAGACAGGGACGGCCGTGTTGTTTTTGGATTTAAACCGTTTCAAAGTGTTTAATGACGCCCTAGGCCACGACGT
CGGGGACCTGCTGCTGGTGGCTGCTGCACAGCGTTTGGCGCACTGTGTGCCCAATGAAGGCTTTATCGCCCGGCTGGGCG
GCGATGAATTTATCATTGTCGCTCCTTTACAAGGGATCGATCAGCTGACCAGGCAATTGATCAGTCAGTTTGAAACGCCT
TTTCGCATTAAAGAGCATCGCCTCAAAACATCCGTCAGCATCGGAATCGCCGTTTCTCCGGACGATGGAACAGACGGCGA
TGAGCTGATGAGAAAAGCCGACATGGCTATGTACGCCGCAAAGCACAAGAGCGTAAGCAGATATCGGTACTTCTCGTCTT
CCCTCGCCAGAAAGAACAGACCGTCTTTCCAGAAGGAAATCGAGCTGAACTAA

Upstream 100 bases:

>100_bases
TTTTTTATGTCAACGGTTAAGAGTACGGCAGTCTAGCCTTTCACAATACAGTATAGTTATTTAGACTTTTCATCATAAGT
ACAAAAAGGCGGAGAAAAAA

Downstream 100 bases:

>100_bases
GGCTTCCTTCAAGAAGAACGGCTCCAAACTTGTACCGCCGCAAGCATCCGGGCTTCAGCCCGGATGCTTTTTTCCTCTGT
CATCCATAAGTTGCTATTCA

Product: YkoW

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 550; Mature: 549

Protein sequence:

>550_residues
MTEVLGEYNGWLVLLSYFTAVLAAYTALDLSERVVHTKGRKNIGWLLAGSLTMGLGIWSMHFIGMVSFQTAEEMKYDLKF
VLLSIVAAFAGSLISFYVVNEKKPTNGRLAAGSLAMGCAIASMHFIGMEAMENMTIVYHPVLYAASFAIAIFASFAALKL
SVVFAKKTGSFRILFIKIGSALLMGGAISGMHYTGMSAATLFMADSVDTGYEGIDTVELGIMIVLVSLMLQCFLIFGAFN
DKRLLLHIEKVKDNEERFQSLINHNIDPIYVFSLEGKLLSANSSGYSLLEKMGIEPERLSSVFRQEDHDRLLGWFLQVQN
EKQAINRDTAIAIAEKRFDLNVTLIPVYVRKQLDSIYVICKDMTNQREAERKIHRMAHYDALTDLPNRRYAVDRLANVLK
RQETGTAVLFLDLNRFKVFNDALGHDVGDLLLVAAAQRLAHCVPNEGFIARLGGDEFIIVAPLQGIDQLTRQLISQFETP
FRIKEHRLKTSVSIGIAVSPDDGTDGDELMRKADMAMYAAKHKSVSRYRYFSSSLARKNRPSFQKEIELN

Sequences:

>Translated_550_residues
MTEVLGEYNGWLVLLSYFTAVLAAYTALDLSERVVHTKGRKNIGWLLAGSLTMGLGIWSMHFIGMVSFQTAEEMKYDLKF
VLLSIVAAFAGSLISFYVVNEKKPTNGRLAAGSLAMGCAIASMHFIGMEAMENMTIVYHPVLYAASFAIAIFASFAALKL
SVVFAKKTGSFRILFIKIGSALLMGGAISGMHYTGMSAATLFMADSVDTGYEGIDTVELGIMIVLVSLMLQCFLIFGAFN
DKRLLLHIEKVKDNEERFQSLINHNIDPIYVFSLEGKLLSANSSGYSLLEKMGIEPERLSSVFRQEDHDRLLGWFLQVQN
EKQAINRDTAIAIAEKRFDLNVTLIPVYVRKQLDSIYVICKDMTNQREAERKIHRMAHYDALTDLPNRRYAVDRLANVLK
RQETGTAVLFLDLNRFKVFNDALGHDVGDLLLVAAAQRLAHCVPNEGFIARLGGDEFIIVAPLQGIDQLTRQLISQFETP
FRIKEHRLKTSVSIGIAVSPDDGTDGDELMRKADMAMYAAKHKSVSRYRYFSSSLARKNRPSFQKEIELN
>Mature_549_residues
TEVLGEYNGWLVLLSYFTAVLAAYTALDLSERVVHTKGRKNIGWLLAGSLTMGLGIWSMHFIGMVSFQTAEEMKYDLKFV
LLSIVAAFAGSLISFYVVNEKKPTNGRLAAGSLAMGCAIASMHFIGMEAMENMTIVYHPVLYAASFAIAIFASFAALKLS
VVFAKKTGSFRILFIKIGSALLMGGAISGMHYTGMSAATLFMADSVDTGYEGIDTVELGIMIVLVSLMLQCFLIFGAFND
KRLLLHIEKVKDNEERFQSLINHNIDPIYVFSLEGKLLSANSSGYSLLEKMGIEPERLSSVFRQEDHDRLLGWFLQVQNE
KQAINRDTAIAIAEKRFDLNVTLIPVYVRKQLDSIYVICKDMTNQREAERKIHRMAHYDALTDLPNRRYAVDRLANVLKR
QETGTAVLFLDLNRFKVFNDALGHDVGDLLLVAAAQRLAHCVPNEGFIARLGGDEFIIVAPLQGIDQLTRQLISQFETPF
RIKEHRLKTSVSIGIAVSPDDGTDGDELMRKADMAMYAAKHKSVSRYRYFSSSLARKNRPSFQKEIELN

Specific function: Probable signaling protein whose physiological role is not yet known [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1787541, Length=191, Percent_Identity=32.4607329842932, Blast_Score=118, Evalue=9e-28,
Organism=Escherichia coli, GI1788381, Length=226, Percent_Identity=29.2035398230088, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI87081977, Length=203, Percent_Identity=30.5418719211823, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87081881, Length=183, Percent_Identity=32.2404371584699, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1788956, Length=161, Percent_Identity=34.7826086956522, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787262, Length=174, Percent_Identity=29.8850574712644, Blast_Score=76, Evalue=7e-15,
Organism=Escherichia coli, GI145693134, Length=170, Percent_Identity=32.3529411764706, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1786584, Length=189, Percent_Identity=26.984126984127, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1787802, Length=165, Percent_Identity=28.4848484848485, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI87082007, Length=193, Percent_Identity=29.0155440414508, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI87081974, Length=151, Percent_Identity=29.8013245033113, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR005330
- InterPro:   IPR000014 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF03707 MHYT [H]

EC number: NA

Molecular weight: Translated: 61444; Mature: 61313

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: PS50924 MHYT ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEVLGEYNGWLVLLSYFTAVLAAYTALDLSERVVHTKGRKNIGWLLAGSLTMGLGIWSM
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHH
HFIGMVSFQTAEEMKYDLKFVLLSIVAAFAGSLISFYVVNEKKPTNGRLAAGSLAMGCAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCEECCHHHHHHHH
ASMHFIGMEAMENMTIVYHPVLYAASFAIAIFASFAALKLSVVFAKKTGSFRILFIKIGS
HHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEEEEEECC
ALLMGGAISGMHYTGMSAATLFMADSVDTGYEGIDTVELGIMIVLVSLMLQCFLIFGAFN
HHHHCCCCCCCEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
DKRLLLHIEKVKDNEERFQSLINHNIDPIYVFSLEGKLLSANSSGYSLLEKMGIEPERLS
CCEEEEEEEHHCCCHHHHHHHHCCCCCEEEEEEECCEEEECCCCHHHHHHHHCCCHHHHH
SVFRQEDHDRLLGWFLQVQNEKQAINRDTAIAIAEKRFDLNVTLIPVYVRKQLDSIYVIC
HHHHCCCHHHHHHHHHHHCCHHHHHCCCCEEEEEEECCCCEEEEEHHHHHHHHHHEEEEE
KDMTNQREAERKIHRMAHYDALTDLPNRRYAVDRLANVLKRQETGTAVLFLDLNRFKVFN
HHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHH
DALGHDVGDLLLVAAAQRLAHCVPNEGFIARLGGDEFIIVAPLQGIDQLTRQLISQFETP
HHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHCCCC
FRIKEHRLKTSVSIGIAVSPDDGTDGDELMRKADMAMYAAKHKSVSRYRYFSSSLARKNR
CHHHHHHHHEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PSFQKEIELN
CCCCCCCCCC
>Mature Secondary Structure 
TEVLGEYNGWLVLLSYFTAVLAAYTALDLSERVVHTKGRKNIGWLLAGSLTMGLGIWSM
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHH
HFIGMVSFQTAEEMKYDLKFVLLSIVAAFAGSLISFYVVNEKKPTNGRLAAGSLAMGCAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCEECCHHHHHHHH
ASMHFIGMEAMENMTIVYHPVLYAASFAIAIFASFAALKLSVVFAKKTGSFRILFIKIGS
HHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEEEEEECC
ALLMGGAISGMHYTGMSAATLFMADSVDTGYEGIDTVELGIMIVLVSLMLQCFLIFGAFN
HHHHCCCCCCCEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
DKRLLLHIEKVKDNEERFQSLINHNIDPIYVFSLEGKLLSANSSGYSLLEKMGIEPERLS
CCEEEEEEEHHCCCHHHHHHHHCCCCCEEEEEEECCEEEECCCCHHHHHHHHCCCHHHHH
SVFRQEDHDRLLGWFLQVQNEKQAINRDTAIAIAEKRFDLNVTLIPVYVRKQLDSIYVIC
HHHHCCCHHHHHHHHHHHCCHHHHHCCCCEEEEEEECCCCEEEEEHHHHHHHHHHEEEEE
KDMTNQREAERKIHRMAHYDALTDLPNRRYAVDRLANVLKRQETGTAVLFLDLNRFKVFN
HHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHH
DALGHDVGDLLLVAAAQRLAHCVPNEGFIARLGGDEFIIVAPLQGIDQLTRQLISQFETP
HHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHCCCC
FRIKEHRLKTSVSIGIAVSPDDGTDGDELMRKADMAMYAAKHKSVSRYRYFSSSLARKNR
CHHHHHHHHEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PSFQKEIELN
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 11728710 [H]