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The map label for this gene is ispA [H]

Identifier: 52785191

GI number: 52785191

Start: 1418622

End: 1419575

Strand: Reverse

Name: ispA [H]

Synonym: BLi01423

Alternate gene names: 52785191

Gene position: 1419575-1418622 (Counterclockwise)

Preceding gene: 52785195

Following gene: 52785190

Centisome position: 33.62

GC content: 50.84

Gene sequence:

>954_bases
ATGCAAATGCGATTAATTCCTTATGTCACTGACACAAAGCTGATGGACGTGAATGAACTGCCGGAAGGAATCGATGCGAT
TAAGGCACCGGAGCTGTGGTCACAAGGTTTTAAAGGAAAAGATATCACTGTCGCAGTGCTTGATACAGGCTGTGACGCAA
CCCATCCAGATTTGGCGGACCGAATCATCGGAGGCAGAAACTTCACTGATGATGACAACGGAAAAGAAGATCAGTTTCAT
GATTATAATGGACATGGCACCCATGTGGCGGGTACGATTGCCGCAAACGATCAAAACGGAGGCATTTCCGGCGTAGCACC
CGAAGCGAACCTATTAATCGTCAAGGTGCTCGGCGGTGAAGACGGCAGCGGGGATTATGAATGGATCATCAACGGGATCA
ACTACGCCGTTGAGCAAAAAGCCGACATTATTTCAATGTCGCTCGGCGGTCCTGCCGACGTTCCGGAGTTGAAGGAAGCG
GTGACAAACGCCGTGAAGAGCGGAGTGCTCGTCGTCTGCGCCGCAGGAAACGAAGGCGACGGCAATGACCGTACAGAGGA
GTACTCATACCCTGCTGCATACAACGAAGTCATCGCCGTCGGATCCGTGTCATTGACGCGTGAGTCTTCCGAATTTTCAA
ATGCGAACAAAGAAATTGACCTTGTTGCACCTGGAGAAGAAATCCTCTCTACATTGCCCGACCATCAATACGGAAAGCTG
ACGGGAACATCGATGGCTACACCGCACGTCAGCGGCGCGCTCGCTCTCATCAAGTCAGCTGAAGAAGAGGCGTTTAAACG
GAAACTGACAGAACCCGAACTGTATGCTCAGTTAATCCGCCGCACCCTTCCTCTTGATTACTCAAAAGCGCTGATCGGCA
ACGGATTCTTATATTTGTCAGCGCCGGAGGTACTGGCGGAAAAAGCCGGCGAAGCAAAACTTCTTTCCCTTTAA

Upstream 100 bases:

>100_bases
TCATACAAATGAACCTTTTTTTTACAAAGTGAATGTTTTACAATAGAATCACGCACCTTGTAACCCATCTAAAGCAGCAT
ATAACAGGGAGGGCTTTGAA

Downstream 100 bases:

>100_bases
CAGTCTAAAGGAGGCTGCCGACAATGTCGGCGGCCTTTTTCATGGCCATGTATAAAGCTGAATCTTTTTAATTGCAAGAA
TTCAAAAATTATTTTGACTA

Product: hypothetical protein

Products: NA

Alternate protein names: ISP-1 [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MQMRLIPYVTDTKLMDVNELPEGIDAIKAPELWSQGFKGKDITVAVLDTGCDATHPDLADRIIGGRNFTDDDNGKEDQFH
DYNGHGTHVAGTIAANDQNGGISGVAPEANLLIVKVLGGEDGSGDYEWIINGINYAVEQKADIISMSLGGPADVPELKEA
VTNAVKSGVLVVCAAGNEGDGNDRTEEYSYPAAYNEVIAVGSVSLTRESSEFSNANKEIDLVAPGEEILSTLPDHQYGKL
TGTSMATPHVSGALALIKSAEEEAFKRKLTEPELYAQLIRRTLPLDYSKALIGNGFLYLSAPEVLAEKAGEAKLLSL

Sequences:

>Translated_317_residues
MQMRLIPYVTDTKLMDVNELPEGIDAIKAPELWSQGFKGKDITVAVLDTGCDATHPDLADRIIGGRNFTDDDNGKEDQFH
DYNGHGTHVAGTIAANDQNGGISGVAPEANLLIVKVLGGEDGSGDYEWIINGINYAVEQKADIISMSLGGPADVPELKEA
VTNAVKSGVLVVCAAGNEGDGNDRTEEYSYPAAYNEVIAVGSVSLTRESSEFSNANKEIDLVAPGEEILSTLPDHQYGKL
TGTSMATPHVSGALALIKSAEEEAFKRKLTEPELYAQLIRRTLPLDYSKALIGNGFLYLSAPEVLAEKAGEAKLLSL
>Mature_317_residues
MQMRLIPYVTDTKLMDVNELPEGIDAIKAPELWSQGFKGKDITVAVLDTGCDATHPDLADRIIGGRNFTDDDNGKEDQFH
DYNGHGTHVAGTIAANDQNGGISGVAPEANLLIVKVLGGEDGSGDYEWIINGINYAVEQKADIISMSLGGPADVPELKEA
VTNAVKSGVLVVCAAGNEGDGNDRTEEYSYPAAYNEVIAVGSVSLTRESSEFSNANKEIDLVAPGEEILSTLPDHQYGKL
TGTSMATPHVSGALALIKSAEEEAFKRKLTEPELYAQLIRRTLPLDYSKALIGNGFLYLSAPEVLAEKAGEAKLLSL

Specific function: Major intracellular protease produced by Bacillus subtilis [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

Organism=Homo sapiens, GI4506775, Length=277, Percent_Identity=29.9638989169675, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI31317307, Length=303, Percent_Identity=27.3927392739274, Blast_Score=66, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6320775, Length=285, Percent_Identity=36.4912280701754, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6319893, Length=288, Percent_Identity=33.6805555555556, Blast_Score=115, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6324576, Length=260, Percent_Identity=33.0769230769231, Blast_Score=100, Evalue=3e-22,
Organism=Drosophila melanogaster, GI45550681, Length=294, Percent_Identity=27.891156462585, Blast_Score=99, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500 [H]

Pfam domain/function: PF00082 Peptidase_S8 [H]

EC number: NA

Molecular weight: Translated: 33795; Mature: 33795

Theoretical pI: Translated: 4.23; Mature: 4.23

Prosite motif: PS00136 SUBTILASE_ASP ; PS00137 SUBTILASE_HIS ; PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQMRLIPYVTDTKLMDVNELPEGIDAIKAPELWSQGFKGKDITVAVLDTGCDATHPDLAD
CCEEEEEEECCCEEECHHHCCHHHHHHCCCHHHHCCCCCCCEEEEEEECCCCCCCCHHHH
RIIGGRNFTDDDNGKEDQFHDYNGHGTHVAGTIAANDQNGGISGVAPEANLLIVKVLGGE
HHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCC
DGSGDYEWIINGINYAVEQKADIISMSLGGPADVPELKEAVTNAVKSGVLVVCAAGNEGD
CCCCCCEEEEECHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCC
GNDRTEEYSYPAAYNEVIAVGSVSLTRESSEFSNANKEIDLVAPGEEILSTLPDHQYGKL
CCCCCCCCCCCCHHHHEEEECCEEEECCCHHHCCCCCEEEEECCCHHHHHHCCCCCCCCC
TGTSMATPHVSGALALIKSAEEEAFKRKLTEPELYAQLIRRTLPLDYSKALIGNGFLYLS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHCCCEEEEE
APEVLAEKAGEAKLLSL
CHHHHHHCCCCEEECCC
>Mature Secondary Structure
MQMRLIPYVTDTKLMDVNELPEGIDAIKAPELWSQGFKGKDITVAVLDTGCDATHPDLAD
CCEEEEEEECCCEEECHHHCCHHHHHHCCCHHHHCCCCCCCEEEEEEECCCCCCCCHHHH
RIIGGRNFTDDDNGKEDQFHDYNGHGTHVAGTIAANDQNGGISGVAPEANLLIVKVLGGE
HHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCC
DGSGDYEWIINGINYAVEQKADIISMSLGGPADVPELKEAVTNAVKSGVLVVCAAGNEGD
CCCCCCEEEEECHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCC
GNDRTEEYSYPAAYNEVIAVGSVSLTRESSEFSNANKEIDLVAPGEEILSTLPDHQYGKL
CCCCCCCCCCCCHHHHEEEECCEEEECCCHHHCCCCCEEEEECCCHHHHHHCCCCCCCCC
TGTSMATPHVSGALALIKSAEEEAFKRKLTEPELYAQLIRRTLPLDYSKALIGNGFLYLS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHCCCEEEEE
APEVLAEKAGEAKLLSL
CHHHHHHCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3087947; 9384377 [H]