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The map label for this gene is metE

Identifier: 52785190

GI number: 52785190

Start: 1415863

End: 1418151

Strand: Reverse

Name: metE

Synonym: BLi01422

Alternate gene names: 52785190

Gene position: 1418151-1415863 (Counterclockwise)

Preceding gene: 52785191

Following gene: 52785189

Centisome position: 33.58

GC content: 47.49

Gene sequence:

>2289_bases
ATGACTAATGTAAAAACGAGCAGCTTGGGCTTTCCAAGAATCGGCTTGAACAGAGAATGGAAAAAATCGCTTGAGGCTTA
TTGGAAAGGAAACACGGACCGCGAGACCTTTTTGAAAGAAATGGATGAACAATTTTTAGCAGCGCTCCAGACTCAGCTTG
ATCAGCAAATCGATATCATACCGGTTTCCGACTTTACAATGTACGACCATGTTCTTGACACGGCGGTGATGTTCAACTGG
ATTCCAGATCGATTCAAGGATATAAACGATCCGTTAGATACTTATTTCGCAATGGCGAGAGGCACGAAAGATGCTGTATC
GAGTGAAATGACAAAATGGTTTAATACAAACTACCATTATATTGTGCCTGAATATGAAAAAGGTGCACAATACCGCGTGA
CGAGAAACAAACCGCTTCAAGATTACCAAAGAGCAAAAGCAGCATTGGGAACAGAAACGAAGCCCGTCATACTCGGCCTT
TACACTTTCGTAGCCCTTGCAAAAGGCTATGAACAACAGGATATTAAAGATATTTATAACCAAATGACACCTCTTTACAT
CCAGGTTTTGAAAGAGCTTGAGCAGGAAGGCGTCAAATGGGTGCAAATTGACGAGCCTGCTCTTGTGACGGCTTCACCTG
AAGAAGCGGCTGCTGTCAAAGAAATCTATCAGACGATTACAGAAGAAGTCTCTGAACTGAACATCCTTCTGCAAACCTAC
TTTGACTCGGTTGATGCTTATGAAGAGCTGATATCGTTTCCTGTCGCAGGAATTGGTCTTGATTTTGTTCATGATAAAGG
GAAAAACTTCGAACACCTGAAAGCGCACGGTTTTCCTAAAGACAAAGTCCTTGCCGCCGGCATTTTAGACGGACGCAACA
TTTGGAAAGCCAATCTCGAAGAGCGCCTCGACCTGACGCTTGAACTGATCCAGAGAGCGGGTGTTGACGAAGTCTGGATT
CAGCCTTCAAACAGCCTGCTTCATGTCCCTGTCGCAAAACACCCGGGCGAACATCTTGCCGACGATCTCTTGAACGGTTT
ATCTTTCGCAAAAGAGAAACTTCTGGAGCTTACACTGCTGAAGAACGGACTTGTTTCCGGAAAAGCGGCCATCCAAGCGG
AAATCGATGAAGCGCACGGACACCTTCAAGATCTCAAACAGTACGGTGCAGCGACAAATTCGGCCTTTGCCGAAGAAAGA
GGCAAGCTGACTGAGGAAGACTTTAAACGCCCGACAGCTTTTGAAGAAAGGCTGCGGATTCAAAATGACTCTCTCGGACT
TCCCCTATTGCCGACAACAACGATCGGCAGCTTCCCGCAGACGGCGGATGTGCGGAGCGCGCGGCAAAAATGGCGGAAAA
AAGAATGGTCCGACGAGCAGTATGAAGCATTTATTCAGGAAGAAACAAAGAAATGGATTGATATTCAGGAAGATCTCGGA
CTTGACGTTCTCGTTCACGGAGAATTCGAACGGACAGACATGGTTGAGTATTTCGGCGAAAAGCTCGGAGGATTCGCCTT
TACTAAATACGCCTGGGTTCAGTCATACGGTTCCCGCTGCGTCCGGCCGCCGGTCATCTACGGAGATGTCGAGTTTAAAG
AGCCGATGACGGTAAAAGAAACGGTTTACGCCCAATCCTTGACCTCGAAGAAAGTCAAGGGCATGCTGACAGGGCCTGTT
ACCATTTTAAACTGGTCCTTTGCCCGCTATGACCTGCCGAGAAAAGAGATCGCCTTCCAAATCGCCTGCGCCCTCCGCAA
AGAGGTTGAAGCGCTTGAAAAAGCAGGAATTCAAATCATTCAGGTCGATGAACCTGCCTTGAGAGAAGGCCTGCCGCTTA
AAGAACGGGATTGGGACGAGTATCTCAAATGGGCTGCAGAAGCGTTCAGACTGTCCACTTCATCTGTGGAAGATACGACG
CAAATCCATACGCATATGTGCTACAGCAACTTTGAAGATATCGTAGACGCGATCGAAGATCTTGACGCAGACGTCATTAC
GATCGAGCACAGCAGAAGCCACGGCGGATTTCTTGATTATCTGGAACAGCACCCTTACCTGAAAGGGCTTGGTCTTGGCG
TATATGATATTCACAGCCCTCGCGTCCCTTCCAGCGATGAAATGCTCACGATCATAGAAGACGCGCTGAAAGTCTGCCCG
GCTGATCGCTTCTGGGTAAACCCTGACTGCGGTTTAAAAACGAGACAGCCAGAGGAAACGATCGCAGCGCTTAAGAATAT
GGTTGAAGCAGCCAAACAAGCAAGAGGCAAACTGGCTCAGACTGTTTAA

Upstream 100 bases:

>100_bases
TATTTCTCGTTCGCAACATGCTGTTTTCAGGCATTCACCTTCTCATTGTCCGAAGTGTGAGTGTCTTTTTTTATTGAACA
CTAAAAGGAGGAGACCAGAC

Downstream 100 bases:

>100_bases
TTTCACAAAAAATCCACTACAAACGCCGCCTGTTCACACGGGCGGCTCTTTTCATGGCTCCAGCCCTTTTTAGGCCAAAA
GAACCGTTATACAAGGTATG

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme

Number of amino acids: Translated: 762; Mature: 761

Protein sequence:

>762_residues
MTNVKTSSLGFPRIGLNREWKKSLEAYWKGNTDRETFLKEMDEQFLAALQTQLDQQIDIIPVSDFTMYDHVLDTAVMFNW
IPDRFKDINDPLDTYFAMARGTKDAVSSEMTKWFNTNYHYIVPEYEKGAQYRVTRNKPLQDYQRAKAALGTETKPVILGL
YTFVALAKGYEQQDIKDIYNQMTPLYIQVLKELEQEGVKWVQIDEPALVTASPEEAAAVKEIYQTITEEVSELNILLQTY
FDSVDAYEELISFPVAGIGLDFVHDKGKNFEHLKAHGFPKDKVLAAGILDGRNIWKANLEERLDLTLELIQRAGVDEVWI
QPSNSLLHVPVAKHPGEHLADDLLNGLSFAKEKLLELTLLKNGLVSGKAAIQAEIDEAHGHLQDLKQYGAATNSAFAEER
GKLTEEDFKRPTAFEERLRIQNDSLGLPLLPTTTIGSFPQTADVRSARQKWRKKEWSDEQYEAFIQEETKKWIDIQEDLG
LDVLVHGEFERTDMVEYFGEKLGGFAFTKYAWVQSYGSRCVRPPVIYGDVEFKEPMTVKETVYAQSLTSKKVKGMLTGPV
TILNWSFARYDLPRKEIAFQIACALRKEVEALEKAGIQIIQVDEPALREGLPLKERDWDEYLKWAAEAFRLSTSSVEDTT
QIHTHMCYSNFEDIVDAIEDLDADVITIEHSRSHGGFLDYLEQHPYLKGLGLGVYDIHSPRVPSSDEMLTIIEDALKVCP
ADRFWVNPDCGLKTRQPEETIAALKNMVEAAKQARGKLAQTV

Sequences:

>Translated_762_residues
MTNVKTSSLGFPRIGLNREWKKSLEAYWKGNTDRETFLKEMDEQFLAALQTQLDQQIDIIPVSDFTMYDHVLDTAVMFNW
IPDRFKDINDPLDTYFAMARGTKDAVSSEMTKWFNTNYHYIVPEYEKGAQYRVTRNKPLQDYQRAKAALGTETKPVILGL
YTFVALAKGYEQQDIKDIYNQMTPLYIQVLKELEQEGVKWVQIDEPALVTASPEEAAAVKEIYQTITEEVSELNILLQTY
FDSVDAYEELISFPVAGIGLDFVHDKGKNFEHLKAHGFPKDKVLAAGILDGRNIWKANLEERLDLTLELIQRAGVDEVWI
QPSNSLLHVPVAKHPGEHLADDLLNGLSFAKEKLLELTLLKNGLVSGKAAIQAEIDEAHGHLQDLKQYGAATNSAFAEER
GKLTEEDFKRPTAFEERLRIQNDSLGLPLLPTTTIGSFPQTADVRSARQKWRKKEWSDEQYEAFIQEETKKWIDIQEDLG
LDVLVHGEFERTDMVEYFGEKLGGFAFTKYAWVQSYGSRCVRPPVIYGDVEFKEPMTVKETVYAQSLTSKKVKGMLTGPV
TILNWSFARYDLPRKEIAFQIACALRKEVEALEKAGIQIIQVDEPALREGLPLKERDWDEYLKWAAEAFRLSTSSVEDTT
QIHTHMCYSNFEDIVDAIEDLDADVITIEHSRSHGGFLDYLEQHPYLKGLGLGVYDIHSPRVPSSDEMLTIIEDALKVCP
ADRFWVNPDCGLKTRQPEETIAALKNMVEAAKQARGKLAQTV
>Mature_761_residues
TNVKTSSLGFPRIGLNREWKKSLEAYWKGNTDRETFLKEMDEQFLAALQTQLDQQIDIIPVSDFTMYDHVLDTAVMFNWI
PDRFKDINDPLDTYFAMARGTKDAVSSEMTKWFNTNYHYIVPEYEKGAQYRVTRNKPLQDYQRAKAALGTETKPVILGLY
TFVALAKGYEQQDIKDIYNQMTPLYIQVLKELEQEGVKWVQIDEPALVTASPEEAAAVKEIYQTITEEVSELNILLQTYF
DSVDAYEELISFPVAGIGLDFVHDKGKNFEHLKAHGFPKDKVLAAGILDGRNIWKANLEERLDLTLELIQRAGVDEVWIQ
PSNSLLHVPVAKHPGEHLADDLLNGLSFAKEKLLELTLLKNGLVSGKAAIQAEIDEAHGHLQDLKQYGAATNSAFAEERG
KLTEEDFKRPTAFEERLRIQNDSLGLPLLPTTTIGSFPQTADVRSARQKWRKKEWSDEQYEAFIQEETKKWIDIQEDLGL
DVLVHGEFERTDMVEYFGEKLGGFAFTKYAWVQSYGSRCVRPPVIYGDVEFKEPMTVKETVYAQSLTSKKVKGMLTGPVT
ILNWSFARYDLPRKEIAFQIACALRKEVEALEKAGIQIIQVDEPALREGLPLKERDWDEYLKWAAEAFRLSTSSVEDTTQ
IHTHMCYSNFEDIVDAIEDLDADVITIEHSRSHGGFLDYLEQHPYLKGLGLGVYDIHSPRVPSSDEMLTIIEDALKVCPA
DRFWVNPDCGLKTRQPEETIAALKNMVEAAKQARGKLAQTV

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family

Homologues:

Organism=Escherichia coli, GI2367304, Length=762, Percent_Identity=46.9816272965879, Blast_Score=644, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=775, Percent_Identity=44.6451612903226, Blast_Score=604, Evalue=1e-173,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METE_BACLD (Q65KT8)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_078611.1
- RefSeq:   YP_091019.1
- HSSP:   Q9X112
- ProteinModelPortal:   Q65KT8
- SMR:   Q65KT8
- STRING:   Q65KT8
- EnsemblBacteria:   EBBACT00000056208
- EnsemblBacteria:   EBBACT00000058876
- GeneID:   3030479
- GeneID:   3098755
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi01422
- KEGG:   bli:BL03738
- NMPDR:   fig|279010.5.peg.876
- eggNOG:   COG0620
- GeneTree:   EBGT00050000001686
- HOGENOM:   HBG287495
- OMA:   RFGWVQS
- ProtClustDB:   PRK05222
- BioCyc:   BLIC279010-1:BLI01422-MONOMER
- BioCyc:   BLIC279010:BL03738-MONOMER
- HAMAP:   MF_00172
- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629
- PIRSF:   PIRSF000382
- TIGRFAMs:   TIGR01371

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2

EC number: =2.1.1.14

Molecular weight: Translated: 86719; Mature: 86588

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNVKTSSLGFPRIGLNREWKKSLEAYWKGNTDRETFLKEMDEQFLAALQTQLDQQIDII
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEE
PVSDFTMYDHVLDTAVMFNWIPDRFKDINDPLDTYFAMARGTKDAVSSEMTKWFNTNYHY
ECCCHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCEE
IVPEYEKGAQYRVTRNKPLQDYQRAKAALGTETKPVILGLYTFVALAKGYEQQDIKDIYN
ECCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
QMTPLYIQVLKELEQEGVKWVQIDEPALVTASPEEAAAVKEIYQTITEEVSELNILLQTY
HHHHHHHHHHHHHHHCCCEEEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDSVDAYEELISFPVAGIGLDFVHDKGKNFEHLKAHGFPKDKVLAAGILDGRNIWKANLE
HHHHHHHHHHHHCCHHHCCHHHHHCCCCCHHHHHHCCCCHHHEEEEECCCCCHHHHHCHH
ERLDLTLELIQRAGVDEVWIQPSNSLLHVPVAKHPGEHLADDLLNGLSFAKEKLLELTLL
HHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KNGLVSGKAAIQAEIDEAHGHLQDLKQYGAATNSAFAEERGKLTEEDFKRPTAFEERLRI
HCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHCCCCCHHHHHHHH
QNDSLGLPLLPTTTIGSFPQTADVRSARQKWRKKEWSDEQYEAFIQEETKKWIDIQEDLG
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHCCC
LDVLVHGEFERTDMVEYFGEKLGGFAFTKYAWVQSYGSRCVRPPVIYGDVEFKEPMTVKE
CEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCHHH
TVYAQSLTSKKVKGMLTGPVTILNWSFARYDLPRKEIAFQIACALRKEVEALEKAGIQII
HHHHHHHHHHHHCCEECCCEEEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEE
QVDEPALREGLPLKERDWDEYLKWAAEAFRLSTSSVEDTTQIHTHMCYSNFEDIVDAIED
EECCHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LDADVITIEHSRSHGGFLDYLEQHPYLKGLGLGVYDIHSPRVPSSDEMLTIIEDALKVCP
CCCCEEEEECCCCCCCHHHHHHHCCCHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHCC
ADRFWVNPDCGLKTRQPEETIAALKNMVEAAKQARGKLAQTV
CCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TNVKTSSLGFPRIGLNREWKKSLEAYWKGNTDRETFLKEMDEQFLAALQTQLDQQIDII
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEE
PVSDFTMYDHVLDTAVMFNWIPDRFKDINDPLDTYFAMARGTKDAVSSEMTKWFNTNYHY
ECCCHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCEE
IVPEYEKGAQYRVTRNKPLQDYQRAKAALGTETKPVILGLYTFVALAKGYEQQDIKDIYN
ECCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
QMTPLYIQVLKELEQEGVKWVQIDEPALVTASPEEAAAVKEIYQTITEEVSELNILLQTY
HHHHHHHHHHHHHHHCCCEEEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDSVDAYEELISFPVAGIGLDFVHDKGKNFEHLKAHGFPKDKVLAAGILDGRNIWKANLE
HHHHHHHHHHHHCCHHHCCHHHHHCCCCCHHHHHHCCCCHHHEEEEECCCCCHHHHHCHH
ERLDLTLELIQRAGVDEVWIQPSNSLLHVPVAKHPGEHLADDLLNGLSFAKEKLLELTLL
HHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KNGLVSGKAAIQAEIDEAHGHLQDLKQYGAATNSAFAEERGKLTEEDFKRPTAFEERLRI
HCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHCCCCCHHHHHHHH
QNDSLGLPLLPTTTIGSFPQTADVRSARQKWRKKEWSDEQYEAFIQEETKKWIDIQEDLG
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHCCC
LDVLVHGEFERTDMVEYFGEKLGGFAFTKYAWVQSYGSRCVRPPVIYGDVEFKEPMTVKE
CEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCHHH
TVYAQSLTSKKVKGMLTGPVTILNWSFARYDLPRKEIAFQIACALRKEVEALEKAGIQII
HHHHHHHHHHHHCCEECCCEEEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEE
QVDEPALREGLPLKERDWDEYLKWAAEAFRLSTSSVEDTTQIHTHMCYSNFEDIVDAIED
EECCHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LDADVITIEHSRSHGGFLDYLEQHPYLKGLGLGVYDIHSPRVPSSDEMLTIIEDALKVCP
CCCCEEEEECCCCCCCHHHHHHHCCCHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHCC
ADRFWVNPDCGLKTRQPEETIAALKNMVEAAKQARGKLAQTV
CCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA