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The map label for this gene is ispF

Identifier: 52783947

GI number: 52783947

Start: 109243

End: 109719

Strand: Direct

Name: ispF

Synonym: BLi00109

Alternate gene names: 52783947

Gene position: 109243-109719 (Clockwise)

Preceding gene: 52783946

Following gene: 52783948

Centisome position: 2.59

GC content: 49.48

Gene sequence:

>477_bases
ATGTTTAGAATAGGCCAAGGATTTGACGTCCATCAACTAGTGGAAGGCAGGCCGCTCATAATCGGGGGAATTACGATTCC
TTACGAAAAAGGCCTTCTCGGACATTCAGATGCAGATGTGCTTTTGCACACGATTGCGGATGCATGTCTCGGTGCCGTCG
GCGAGGGCGATATCGGAAAACACTTTCCGGATACCGATCCTGAATTCAAAGATGCCGACTCCTTCAAACTCCTTCGCCAC
GTCTGGATGATCGTGAAGGAAAAAGGCTATACGCTAGGGAACCTGGATTGTACAATCATCGCCCAAAAGCCTAAAATGGC
GCCGTATATCGAAGACATGAGAGCCAGAATTGCCGAAGGGCTAGAGGCGGAAATTTCCCAAATTAACGTAAAAGCGACCA
CAACCGAAAAGCTCGGATTTACGGGAAGAGCCGAAGGAATCGCCGCCCAGGCTTCCGTTCTGCTCCAAAAGGTATAA

Upstream 100 bases:

>100_bases
GTTGCAATCATTCAAGGGGATTATCAAAACATCAAACTGACGACCCCGGATGACTTGCTTGTCGCAGAAGCGATATTAGA
AGCGGAAAAGAGGAATGAAC

Downstream 100 bases:

>100_bases
TTCATTTTGAAGACAGGTTCATTTGATGATAGAATAGGAAATAAATTTGAACTCTGCTAGAGACGAAAGGAAGTTAGACA
GATGGGAAACGAAGTTCGGG

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MFRIGQGFDVHQLVEGRPLIIGGITIPYEKGLLGHSDADVLLHTIADACLGAVGEGDIGKHFPDTDPEFKDADSFKLLRH
VWMIVKEKGYTLGNLDCTIIAQKPKMAPYIEDMRARIAEGLEAEISQINVKATTTEKLGFTGRAEGIAAQASVLLQKV

Sequences:

>Translated_158_residues
MFRIGQGFDVHQLVEGRPLIIGGITIPYEKGLLGHSDADVLLHTIADACLGAVGEGDIGKHFPDTDPEFKDADSFKLLRH
VWMIVKEKGYTLGNLDCTIIAQKPKMAPYIEDMRARIAEGLEAEISQINVKATTTEKLGFTGRAEGIAAQASVLLQKV
>Mature_158_residues
MFRIGQGFDVHQLVEGRPLIIGGITIPYEKGLLGHSDADVLLHTIADACLGAVGEGDIGKHFPDTDPEFKDADSFKLLRH
VWMIVKEKGYTLGNLDCTIIAQKPKMAPYIEDMRARIAEGLEAEISQINVKATTTEKLGFTGRAEGIAAQASVLLQKV

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=156, Percent_Identity=64.1025641025641, Blast_Score=186, Evalue=7e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_BACLD (Q65PD1)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_077377.1
- RefSeq:   YP_089776.1
- ProteinModelPortal:   Q65PD1
- SMR:   Q65PD1
- STRING:   Q65PD1
- EnsemblBacteria:   EBBACT00000055607
- EnsemblBacteria:   EBBACT00000060314
- GeneID:   3028340
- GeneID:   3099418
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi00109
- KEGG:   bli:BL03266
- NMPDR:   fig|279010.5.peg.1486
- eggNOG:   COG0245
- GeneTree:   EBGT00050000002053
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- ProtClustDB:   PRK00084
- BioCyc:   BLIC279010-1:BLI00109-MONOMER
- BioCyc:   BLIC279010:BL03266-MONOMER
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 17188; Mature: 17188

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRIGQGFDVHQLVEGRPLIIGGITIPYEKGLLGHSDADVLLHTIADACLGAVGEGDIGK
CCCCCCCCCHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
HFPDTDPEFKDADSFKLLRHVWMIVKEKGYTLGNLDCTIIAQKPKMAPYIEDMRARIAEG
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCEEEEEECCCCCCHHHHHHHHHHHCC
LEAEISQINVKATTTEKLGFTGRAEGIAAQASVLLQKV
HHHHHHHEEEEEECHHHCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MFRIGQGFDVHQLVEGRPLIIGGITIPYEKGLLGHSDADVLLHTIADACLGAVGEGDIGK
CCCCCCCCCHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
HFPDTDPEFKDADSFKLLRHVWMIVKEKGYTLGNLDCTIIAQKPKMAPYIEDMRARIAEG
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCEEEEEECCCCCCHHHHHHHHHHHCC
LEAEISQINVKATTTEKLGFTGRAEGIAAQASVLLQKV
HHHHHHHEEEEEECHHHCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA