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The map label for this gene is ispD

Identifier: 52783946

GI number: 52783946

Start: 108555

End: 109250

Strand: Direct

Name: ispD

Synonym: BLi00108

Alternate gene names: 52783946

Gene position: 108555-109250 (Clockwise)

Preceding gene: 52783945

Following gene: 52783947

Centisome position: 2.57

GC content: 46.41

Gene sequence:

>696_bases
ATGAATTATGAAGTCGTCATTCCTGCCGCCGGACAAGGTAAACGAATGAATGCCGGGAAAAACAAACTGTTTATCGAGGC
GAAAGGAATACCTGTGATTATTCATACATTAAAGGTGTTTGAAAACCATGATGCCTGCAAAAGGATCATTCTTGTCATCA
ATGAACAGGAATTCGGTGAATTCAACACCCTTTTAAAAACCTATCATTTTAAGACTCCGATCGAGATGGTGCCGGGCGGA
AGAGAACGCCAGCAAAGCGTTTTTGCGGGCATTAAAGCGGCCGGCAAAGAGGAAACGGTCCTCGTCCATGACGGAGCAAG
ACCTTTTATTAAACGGGAATATATCGAACAGCTTGTCGAGAAAGCAAAGGAAACAGGGGCAGCCATCGTTGCCGTTCCTG
TTAAAGACACGATCAAACGCGTTCAAGACGGTGAGATAGCCGGAACGATTGAACGTTCAAGCTTGTGGGCCGCTCAAACC
CCACAAGCTTTTCGTCTTTCTATTCTGATGAATGCTCACCTTGAGGCTGAAGCAAAAGGTTTCCTCGGTACGGACGACGC
CAGTCTTGTCGAGGAAGCCGGAGGTACGGTTGCAATCATTCAAGGGGATTATCAAAACATCAAACTGACGACCCCGGATG
ACTTGCTTGTCGCAGAAGCGATATTAGAAGCGGAAAAGAGGAATGAACATGTTTAG

Upstream 100 bases:

>100_bases
GACGTGCTCGTGACCAGCGTGCTGCAGACAGCGGCAGGCAGAATGATATTCGCCAAGCCGAAACTGCTGGAAAAGGCGCT
TTGAAAAAGGGAGAAATAAA

Downstream 100 bases:

>100_bases
AATAGGCCAAGGATTTGACGTCCATCAACTAGTGGAAGGCAGGCCGCTCATAATCGGGGGAATTACGATTCCTTACGAAA
AAGGCCTTCTCGGACATTCA

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MNYEVVIPAAGQGKRMNAGKNKLFIEAKGIPVIIHTLKVFENHDACKRIILVINEQEFGEFNTLLKTYHFKTPIEMVPGG
RERQQSVFAGIKAAGKEETVLVHDGARPFIKREYIEQLVEKAKETGAAIVAVPVKDTIKRVQDGEIAGTIERSSLWAAQT
PQAFRLSILMNAHLEAEAKGFLGTDDASLVEEAGGTVAIIQGDYQNIKLTTPDDLLVAEAILEAEKRNEHV

Sequences:

>Translated_231_residues
MNYEVVIPAAGQGKRMNAGKNKLFIEAKGIPVIIHTLKVFENHDACKRIILVINEQEFGEFNTLLKTYHFKTPIEMVPGG
RERQQSVFAGIKAAGKEETVLVHDGARPFIKREYIEQLVEKAKETGAAIVAVPVKDTIKRVQDGEIAGTIERSSLWAAQT
PQAFRLSILMNAHLEAEAKGFLGTDDASLVEEAGGTVAIIQGDYQNIKLTTPDDLLVAEAILEAEKRNEHV
>Mature_231_residues
MNYEVVIPAAGQGKRMNAGKNKLFIEAKGIPVIIHTLKVFENHDACKRIILVINEQEFGEFNTLLKTYHFKTPIEMVPGG
RERQQSVFAGIKAAGKEETVLVHDGARPFIKREYIEQLVEKAKETGAAIVAVPVKDTIKRVQDGEIAGTIERSSLWAAQT
PQAFRLSILMNAHLEAEAKGFLGTDDASLVEEAGGTVAIIQGDYQNIKLTTPDDLLVAEAILEAEKRNEHV

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=250, Percent_Identity=28.4, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI157671913, Length=132, Percent_Identity=30.3030303030303, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1789104, Length=226, Percent_Identity=36.283185840708, Blast_Score=131, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_BACLD (Q65PD2)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_077376.1
- RefSeq:   YP_089775.1
- ProteinModelPortal:   Q65PD2
- SMR:   Q65PD2
- STRING:   Q65PD2
- EnsemblBacteria:   EBBACT00000058168
- EnsemblBacteria:   EBBACT00000061556
- GeneID:   3028811
- GeneID:   3099001
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi00108
- KEGG:   bli:BL03265
- NMPDR:   fig|279010.5.peg.1485
- eggNOG:   COG1211
- GeneTree:   EBGT00050000001852
- HOGENOM:   HBG672839
- OMA:   PSNIKVT
- ProtClustDB:   PRK00155
- BioCyc:   BLIC279010-1:BLI00108-MONOMER
- BioCyc:   BLIC279010:BL03265-MONOMER
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 25431; Mature: 25431

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYEVVIPAAGQGKRMNAGKNKLFIEAKGIPVIIHTLKVFENHDACKRIILVINEQEFGE
CCEEEEEECCCCCCCCCCCCCEEEEEECCCEEEEEEHHHHCCCCCCEEEEEEECCCCCCH
FNTLLKTYHFKTPIEMVPGGRERQQSVFAGIKAAGKEETVLVHDGARPFIKREYIEQLVE
HHHHHHHHCCCCCHHCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHH
KAKETGAAIVAVPVKDTIKRVQDGEIAGTIERSSLWAAQTPQAFRLSILMNAHLEAEAKG
HHHHCCCEEEEEECHHHHHHHCCCCEEEEEECCCEECCCCCCCEEEEEEEECCCCCCCCC
FLGTDDASLVEEAGGTVAIIQGDYQNIKLTTPDDLLVAEAILEAEKRNEHV
CCCCCCHHHHHHCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNYEVVIPAAGQGKRMNAGKNKLFIEAKGIPVIIHTLKVFENHDACKRIILVINEQEFGE
CCEEEEEECCCCCCCCCCCCCEEEEEECCCEEEEEEHHHHCCCCCCEEEEEEECCCCCCH
FNTLLKTYHFKTPIEMVPGGRERQQSVFAGIKAAGKEETVLVHDGARPFIKREYIEQLVE
HHHHHHHHCCCCCHHCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHH
KAKETGAAIVAVPVKDTIKRVQDGEIAGTIERSSLWAAQTPQAFRLSILMNAHLEAEAKG
HHHHCCCEEEEEECHHHHHHHCCCCEEEEEECCCEECCCCCCCEEEEEEEECCCCCCCCC
FLGTDDASLVEEAGGTVAIIQGDYQNIKLTTPDDLLVAEAILEAEKRNEHV
CCCCCCHHHHHHCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA