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The map label for this gene is gltX

Identifier: 52783948

GI number: 52783948

Start: 109801

End: 111258

Strand: Direct

Name: gltX

Synonym: BLi00110

Alternate gene names: 52783948

Gene position: 109801-111258 (Clockwise)

Preceding gene: 52783947

Following gene: 52783949

Centisome position: 2.6

GC content: 46.36

Gene sequence:

>1458_bases
ATGGGAAACGAAGTTCGGGTTCGTTATGCGCCGAGCCCAACAGGCCATTTACATATCGGAAATGCCAGAACCGCTCTTTT
TAATTATTTGTTTGCCCGGAGCCAAGGCGGAAAATTCATCATCCGCATTGAAGATACGGACCGCAAGCGCAACATTGAAG
GCGGGGAGCAAAGCCAGCTGAACTATTTAAAATGGCTGGGCATCGACTGGGATGAAAGCGTGGATGTCGGAGGAGAATAC
GGACCATACCGCCAATCCGAAAGAAACGACATTTACAAAACATATTACGAGGAGCTTCTTGAAAAAGGGCTAGCCTACAA
ATGCTACTGCACGGAAGAAGAGCTTGAGAAAGAGCGTGAGGAACAGGCTGCCCGCGGAGAAATGCCGCGCTACTCCGGCA
AGTGCCGCAATTTAACAAAAGAAGAGCAGGAAAAGCTCGAAGCTGAAGGAAGACAGCCGAGCATCCGCTTTAAAGTGCCT
CAAGGTGAAGTCATCCGCTTTGATGACATCGTCAAAGGAGAAATTTCTTTTGAAACAGACGGAATCGGCGACTTCGTCAT
CGTGAAGAAAGACGGAACGCCTACTTACAACTTTGCCGTAGCGGTCGATGACTATTTAATGAAAATGACGCATGTCCTGC
GCGGCGAAGACCACATTTCCAATACGCCAAAGCAGATCATGATTTACAACGCCCTTGGCTGGGATATCCCGGCATTTGGA
CATATGACATTAATCGTCAATGAAAGCCGCAAAAAACTGAGCAAACGTGATGAGTCCATCATTCAGTTTATCGAGCAGTA
CGAAGAGCTCGGCTATTTGCCGGAAGCATTGTTTAACTTCATCACTCTTCTAGGCTGGTCGCCGGTCGGAGAAGAAGAAC
TGTTTACAAAAGAACAGTTTATTGAGATATTTGATGTAAACCGTTTGTCAAAATCACCAGCGGTGTTTGATACGCATAAA
CTGAAATGGGTCAACAACCAGTATGTGAAAAAACTTGATCTTGATCAGGTCATCGAATTGACCGTGCCGCATTTGCAAAA
AGCGGGCAAGGTTTCAGAAGAGCTTTCAGGCAGCGAACAGGAATGGGTGCGCAAGCTGATTTCCCTATATCAGGAACAGC
TGAGCTACGGCGCTGAAATCGTCGAGTTAACAGAGTTGTTCTTTAAAGACGATATCCAGTATAATCGTGAAGCAAGAACA
GTTTTAGAAGAAGAGCAGGTTCCGGAAGTGCTTCGCGTATTTGCCGAAAAGCTTGAGCAGCTTGACTCGTTTACGGCAGA
TGAGATCAAGGCATCCATCAAAGCCGTTCAAAAAGAAACAGGACATAAAGGGAAAAAGCTGTTTATGCCGATCCGCGTAG
CGACGACAGGCCAGACCCACGGTCCTGAACTGCCTCAGTCCATTGAATTATTGGGGAAAGACACCGTTTTAAAACGGCTG
CATAACATTATTCAATAA

Upstream 100 bases:

>100_bases
TCTGCTCCAAAAGGTATAATTCATTTTGAAGACAGGTTCATTTGATGATAGAATAGGAAATAAATTTGAACTCTGCTAGA
GACGAAAGGAAGTTAGACAG

Downstream 100 bases:

>100_bases
GATAAATCGTTGACAGGGATAAGTATAAAGCCAGGGCCTTTAAAGAGAGAGTCTTCATCGGCTGGAAAAGACTTAAGCGC
CGGGCTTTAGAAATGCACCT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MGNEVRVRYAPSPTGHLHIGNARTALFNYLFARSQGGKFIIRIEDTDRKRNIEGGEQSQLNYLKWLGIDWDESVDVGGEY
GPYRQSERNDIYKTYYEELLEKGLAYKCYCTEEELEKEREEQAARGEMPRYSGKCRNLTKEEQEKLEAEGRQPSIRFKVP
QGEVIRFDDIVKGEISFETDGIGDFVIVKKDGTPTYNFAVAVDDYLMKMTHVLRGEDHISNTPKQIMIYNALGWDIPAFG
HMTLIVNESRKKLSKRDESIIQFIEQYEELGYLPEALFNFITLLGWSPVGEEELFTKEQFIEIFDVNRLSKSPAVFDTHK
LKWVNNQYVKKLDLDQVIELTVPHLQKAGKVSEELSGSEQEWVRKLISLYQEQLSYGAEIVELTELFFKDDIQYNREART
VLEEEQVPEVLRVFAEKLEQLDSFTADEIKASIKAVQKETGHKGKKLFMPIRVATTGQTHGPELPQSIELLGKDTVLKRL
HNIIQ

Sequences:

>Translated_485_residues
MGNEVRVRYAPSPTGHLHIGNARTALFNYLFARSQGGKFIIRIEDTDRKRNIEGGEQSQLNYLKWLGIDWDESVDVGGEY
GPYRQSERNDIYKTYYEELLEKGLAYKCYCTEEELEKEREEQAARGEMPRYSGKCRNLTKEEQEKLEAEGRQPSIRFKVP
QGEVIRFDDIVKGEISFETDGIGDFVIVKKDGTPTYNFAVAVDDYLMKMTHVLRGEDHISNTPKQIMIYNALGWDIPAFG
HMTLIVNESRKKLSKRDESIIQFIEQYEELGYLPEALFNFITLLGWSPVGEEELFTKEQFIEIFDVNRLSKSPAVFDTHK
LKWVNNQYVKKLDLDQVIELTVPHLQKAGKVSEELSGSEQEWVRKLISLYQEQLSYGAEIVELTELFFKDDIQYNREART
VLEEEQVPEVLRVFAEKLEQLDSFTADEIKASIKAVQKETGHKGKKLFMPIRVATTGQTHGPELPQSIELLGKDTVLKRL
HNIIQ
>Mature_484_residues
GNEVRVRYAPSPTGHLHIGNARTALFNYLFARSQGGKFIIRIEDTDRKRNIEGGEQSQLNYLKWLGIDWDESVDVGGEYG
PYRQSERNDIYKTYYEELLEKGLAYKCYCTEEELEKEREEQAARGEMPRYSGKCRNLTKEEQEKLEAEGRQPSIRFKVPQ
GEVIRFDDIVKGEISFETDGIGDFVIVKKDGTPTYNFAVAVDDYLMKMTHVLRGEDHISNTPKQIMIYNALGWDIPAFGH
MTLIVNESRKKLSKRDESIIQFIEQYEELGYLPEALFNFITLLGWSPVGEEELFTKEQFIEIFDVNRLSKSPAVFDTHKL
KWVNNQYVKKLDLDQVIELTVPHLQKAGKVSEELSGSEQEWVRKLISLYQEQLSYGAEIVELTELFFKDDIQYNREARTV
LEEEQVPEVLRVFAEKLEQLDSFTADEIKASIKAVQKETGHKGKKLFMPIRVATTGQTHGPELPQSIELLGKDTVLKRLH
NIIQ

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=501, Percent_Identity=33.1337325349301, Blast_Score=273, Evalue=2e-73,
Organism=Homo sapiens, GI4826960, Length=300, Percent_Identity=24, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788743, Length=481, Percent_Identity=37.8378378378378, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI226510925, Length=253, Percent_Identity=34.3873517786561, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1786895, Length=219, Percent_Identity=30.1369863013699, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71993394, Length=483, Percent_Identity=33.5403726708075, Blast_Score=266, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6324540, Length=510, Percent_Identity=35.2941176470588, Blast_Score=273, Evalue=3e-74,
Organism=Saccharomyces cerevisiae, GI6321192, Length=220, Percent_Identity=29.5454545454545, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324742, Length=230, Percent_Identity=25.2173913043478, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24665321, Length=495, Percent_Identity=35.959595959596, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI17864488, Length=308, Percent_Identity=25.3246753246753, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_BACLD (Q65PD0)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_077378.1
- RefSeq:   YP_089777.1
- ProteinModelPortal:   Q65PD0
- SMR:   Q65PD0
- STRING:   Q65PD0
- EnsemblBacteria:   EBBACT00000055900
- EnsemblBacteria:   EBBACT00000059426
- GeneID:   3027316
- GeneID:   3097740
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi00110
- KEGG:   bli:BL03267
- NMPDR:   fig|279010.5.peg.1487
- eggNOG:   COG0008
- GeneTree:   EBGT00050000002239
- HOGENOM:   HBG628189
- OMA:   NGYPSYN
- ProtClustDB:   PRK01406
- BioCyc:   BLIC279010-1:BLI00110-MONOMER
- BioCyc:   BLIC279010:BL03267-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 56027; Mature: 55896

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 255-255

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNEVRVRYAPSPTGHLHIGNARTALFNYLFARSQGGKFIIRIEDTDRKRNIEGGEQSQL
CCCCEEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHH
NYLKWLGIDWDESVDVGGEYGPYRQSERNDIYKTYYEELLEKGLAYKCYCTEEELEKERE
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECHHHHHHHHH
EQAARGEMPRYSGKCRNLTKEEQEKLEAEGRQPSIRFKVPQGEVIRFDDIVKGEISFETD
HHHHHCCCCCCCCHHCCCCHHHHHHHHHCCCCCCEEEECCCCCEEEECCEEECEEEEECC
GIGDFVIVKKDGTPTYNFAVAVDDYLMKMTHVLRGEDHISNTPKQIMIYNALGWDIPAFG
CCCCEEEEECCCCCCEEEEEEHHHHHHHHHHHHCCCHHCCCCCCEEEEEEECCCCCCCCC
HMTLIVNESRKKLSKRDESIIQFIEQYEELGYLPEALFNFITLLGWSPVGEEELFTKEQF
CEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHH
IEIFDVNRLSKSPAVFDTHKLKWVNNQYVKKLDLDQVIELTVPHLQKAGKVSEELSGSEQ
HHHHHHHHCCCCCCEECCHHEEECCCHHHHHCCHHHHHHHHHHHHHHHCCHHHHHCCCHH
EWVRKLISLYQEQLSYGAEIVELTELFFKDDIQYNREARTVLEEEQVPEVLRVFAEKLEQ
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LDSFTADEIKASIKAVQKETGHKGKKLFMPIRVATTGQTHGPELPQSIELLGKDTVLKRL
HHCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
HNIIQ
HHHCC
>Mature Secondary Structure 
GNEVRVRYAPSPTGHLHIGNARTALFNYLFARSQGGKFIIRIEDTDRKRNIEGGEQSQL
CCCEEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHH
NYLKWLGIDWDESVDVGGEYGPYRQSERNDIYKTYYEELLEKGLAYKCYCTEEELEKERE
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECHHHHHHHHH
EQAARGEMPRYSGKCRNLTKEEQEKLEAEGRQPSIRFKVPQGEVIRFDDIVKGEISFETD
HHHHHCCCCCCCCHHCCCCHHHHHHHHHCCCCCCEEEECCCCCEEEECCEEECEEEEECC
GIGDFVIVKKDGTPTYNFAVAVDDYLMKMTHVLRGEDHISNTPKQIMIYNALGWDIPAFG
CCCCEEEEECCCCCCEEEEEEHHHHHHHHHHHHCCCHHCCCCCCEEEEEEECCCCCCCCC
HMTLIVNESRKKLSKRDESIIQFIEQYEELGYLPEALFNFITLLGWSPVGEEELFTKEQF
CEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHH
IEIFDVNRLSKSPAVFDTHKLKWVNNQYVKKLDLDQVIELTVPHLQKAGKVSEELSGSEQ
HHHHHHHHCCCCCCEECCHHEEECCCHHHHHCCHHHHHHHHHHHHHHHCCHHHHHCCCHH
EWVRKLISLYQEQLSYGAEIVELTELFFKDDIQYNREARTVLEEEQVPEVLRVFAEKLEQ
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LDSFTADEIKASIKAVQKETGHKGKKLFMPIRVATTGQTHGPELPQSIELLGKDTVLKRL
HHCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
HNIIQ
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA