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The map label for this gene is yacD [H]

Identifier: 52783929

GI number: 52783929

Start: 85406

End: 86296

Strand: Direct

Name: yacD [H]

Synonym: BLi00088

Alternate gene names: 52783929

Gene position: 85406-86296 (Clockwise)

Preceding gene: 52783928

Following gene: 52783930

Centisome position: 2.02

GC content: 42.31

Gene sequence:

>891_bases
TTGAAATCGAGAACACTTTGGCCGATTATTTTAGGTGCGATCTTCGTCAATTGCATTGTGATCGCGTATGTTCTGACGAA
AGCGCAGACCGCTTCTTCATCGTCAGCTCAAGAAGTCATTGCCCATATCGGAAAAAACGGCATCACGCGTGAAGAATGGC
TGAGCGAAATGGAAGAGCGCTACGGAAAATCAACCCTTGAAGATATGATCAACGACCGCGTAGTTACCCAAATGGCCGAA
AAAAACAAGATCAAAGTGTCTGATGATGACATTGAAAGAGAATTTCTGCTGATTAAAGCTGTATACAATTCCTTCTACGA
AGACGAAAACACAACAGAAAAAGAATGGAAAGAGCAGATCAAGCATAATATTCTTCTTGAAGAGCTCCTGACAAAAGACA
TTGTCATTTCTGACAGCGAATTGAAGTCATTCTATAACAAAAATAAGGACTTATATAAATTCGATGATTCTTATCGCCTC
AAGCATATCGTTGTCAAAGATAAAGATGAAGCAGAACAAGTTCTTAAAGAACTGAAGGAAGGGTCCAGTTTTGAAGCGGC
CGCAGCCGAACACTCGACTGACCGGTATACGGCTCCTTACGGCGGCGACCTCGGTTTCGTCAATGATCAGCATGAAAACG
TTCCGGCCGAATATTTGGAGAAAGCTGAACAACTAAAGGAAAATCAATGGGTGGATGAACCGATTAAAACAAGCCAGGGC
TATGCGATTATCCAGCTCAAAGAAAAGCTCCCGGGGCAAGCTTTCACTTACAGCGAAGTGAAAAATCAAATCAGAAGGCA
GATCGCGATGGATCAATTAGGCGACAAAGCTTCTGTCAAGACGCTCTGGAAAGATGCGAAACTGTCTTGGTTTTATGACG
ATAAAAAATAA

Upstream 100 bases:

>100_bases
TATCTGTTTACAAAGGAAGAGCTTGAAGCGCTTCGAGATGAAATATAAGCCCGCTATCCAGTAGCGGGTCTTTTACAATG
GGGAAAGGGGAGACACCATA

Downstream 100 bases:

>100_bases
AGATTGACAAAATATTTTGATATTGATAAATTAGATATAATCCCAATAAAATTACTCGGAAATTGAGGTGTTCAAATGGT
TCGCGTTGCAAACTCTGTTT

Product: YacD

Products: peptidylproline (omega=0) [C]

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MKSRTLWPIILGAIFVNCIVIAYVLTKAQTASSSSAQEVIAHIGKNGITREEWLSEMEERYGKSTLEDMINDRVVTQMAE
KNKIKVSDDDIEREFLLIKAVYNSFYEDENTTEKEWKEQIKHNILLEELLTKDIVISDSELKSFYNKNKDLYKFDDSYRL
KHIVVKDKDEAEQVLKELKEGSSFEAAAAEHSTDRYTAPYGGDLGFVNDQHENVPAEYLEKAEQLKENQWVDEPIKTSQG
YAIIQLKEKLPGQAFTYSEVKNQIRRQIAMDQLGDKASVKTLWKDAKLSWFYDDKK

Sequences:

>Translated_296_residues
MKSRTLWPIILGAIFVNCIVIAYVLTKAQTASSSSAQEVIAHIGKNGITREEWLSEMEERYGKSTLEDMINDRVVTQMAE
KNKIKVSDDDIEREFLLIKAVYNSFYEDENTTEKEWKEQIKHNILLEELLTKDIVISDSELKSFYNKNKDLYKFDDSYRL
KHIVVKDKDEAEQVLKELKEGSSFEAAAAEHSTDRYTAPYGGDLGFVNDQHENVPAEYLEKAEQLKENQWVDEPIKTSQG
YAIIQLKEKLPGQAFTYSEVKNQIRRQIAMDQLGDKASVKTLWKDAKLSWFYDDKK
>Mature_296_residues
MKSRTLWPIILGAIFVNCIVIAYVLTKAQTASSSSAQEVIAHIGKNGITREEWLSEMEERYGKSTLEDMINDRVVTQMAE
KNKIKVSDDDIEREFLLIKAVYNSFYEDENTTEKEWKEQIKHNILLEELLTKDIVISDSELKSFYNKNKDLYKFDDSYRL
KHIVVKDKDEAEQVLKELKEGSSFEAAAAEHSTDRYTAPYGGDLGFVNDQHENVPAEYLEKAEQLKENQWVDEPIKTSQG
YAIIQLKEKLPGQAFTYSEVKNQIRRQIAMDQLGDKASVKTLWKDAKLSWFYDDKK

Specific function: Ppiases Accelerate The Folding Of Proteins. Seems To Be Involved In The Folding Of Outer Membrane Proteins. Its Preference At The P1 Position Of The Peptide Substrate Is Glu > Leu > Ala > His > Val > Phe > Ile > Gly > Lys > Thr. [C]

COG id: COG0760

COG function: function code O; Parvulin-like peptidyl-prolyl isomerase

Gene ontology:

Cell location: Type II Membrane Protein [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PpiC domain [H]

Homologues:

Organism=Escherichia coli, GI1786645, Length=165, Percent_Identity=30.9090909090909, Blast_Score=71, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000297
- InterPro:   IPR023058
- InterPro:   IPR008880 [H]

Pfam domain/function: PF00639 Rotamase [H]

EC number: 5.2.1.8 [C]

Molecular weight: Translated: 34256; Mature: 34256

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS01096 PPIC_PPIASE_1 ; PS50198 PPIC_PPIASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSRTLWPIILGAIFVNCIVIAYVLTKAQTASSSSAQEVIAHIGKNGITREEWLSEMEER
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
YGKSTLEDMINDRVVTQMAEKNKIKVSDDDIEREFLLIKAVYNSFYEDENTTEKEWKEQI
HCHHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
KHNILLEELLTKDIVISDSELKSFYNKNKDLYKFDDSYRLKHIVVKDKDEAEQVLKELKE
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEECCCCCCEEEEEECCCHHHHHHHHHHHC
GSSFEAAAAEHSTDRYTAPYGGDLGFVNDQHENVPAEYLEKAEQLKENQWVDEPIKTSQG
CCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHCCCC
YAIIQLKEKLPGQAFTYSEVKNQIRRQIAMDQLGDKASVKTLWKDAKLSWFYDDKK
EEEEEEHHHCCCCCEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEECCCC
>Mature Secondary Structure
MKSRTLWPIILGAIFVNCIVIAYVLTKAQTASSSSAQEVIAHIGKNGITREEWLSEMEER
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
YGKSTLEDMINDRVVTQMAEKNKIKVSDDDIEREFLLIKAVYNSFYEDENTTEKEWKEQI
HCHHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
KHNILLEELLTKDIVISDSELKSFYNKNKDLYKFDDSYRLKHIVVKDKDEAEQVLKELKE
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEECCCCCCEEEEEECCCHHHHHHHHHHHC
GSSFEAAAAEHSTDRYTAPYGGDLGFVNDQHENVPAEYLEKAEQLKENQWVDEPIKTSQG
CCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHCCCC
YAIIQLKEKLPGQAFTYSEVKNQIRRQIAMDQLGDKASVKTLWKDAKLSWFYDDKK
EEEEEEHHHCCCCCEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Peptidoproline (omega=180) [C]

Specific reaction: Peptidoproline (omega=180) = peptidylproline (omega=0) [C]

General reaction: Rotation (Single bond) [C]

Inhibitor: cyclosporins [C]

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]