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The map label for this gene is cysK [H]

Identifier: 52783930

GI number: 52783930

Start: 86372

End: 87295

Strand: Direct

Name: cysK [H]

Synonym: BLi00089

Alternate gene names: 52783930

Gene position: 86372-87295 (Clockwise)

Preceding gene: 52783929

Following gene: 52783931

Centisome position: 2.05

GC content: 46.32

Gene sequence:

>924_bases
ATGGTTCGCGTTGCAAACTCTGTTTATGACTTAATCGGAAATACACCTGTCGTTAAATTAAATCGACTAGTTGATGAAGA
CAGTGCTGAAGTATATGTGAAACTGGAGTATATGAACCCTGGAAGCAGCGTAAAAGACCGGATTGCGCTTGCTATGATAG
AAGACGCTGAAGAGAAAGGCCTTCTGAAAGAAGGAGATACACTGATTGAACCGACAAGCGGAAATACAGGAATCGGACTT
GCCATGGTCGCTGCAGCGAAAGGCATCAAAGCGATTCTTGTTATGCCTGATACAATGAGTTCTGAGCGCCGCAACCTTCT
GCGTGCATATGGAGCTGAGCTTGTGCTGACTCCAGGTGCTGAGGGAATGAAAGGCGCGATTAAAAAAGCGGAAGAACTGG
CTGAGGAACACGGCTATTTTATGCCTCAGCAATTTAACAATCCTGCGAACGCAGAAGTTCACCGCCGCACGACGGGAAAA
GAAATCGCTGAACAATTCGGAGACGGTTTAGATGCTTTCATTGCAGGTATCGGAACCGGCGGAACGATAACGGGTGCCGG
AGAAGTTCTGAAAGAAAAAAATCCGTCCATCAAGATTTTCGCGGTGGAACCGACTGATTCTCCTGTTCTTTCCGGAGGAA
AACCAGGGCCGCACAAAATTCAGGGAATCGGAGCGGGATTCGTTCCGAAAATCTTGAATACGGAAATTTACGATGAAATC
ATCACAGTGAAAAACGAAGAAGCCTTTGAATTTGCCCGTAAAGCGGCGAGAGAGGAAGGAATTCTCGGAGGAATTTCTTC
AGGCGCAGCCATCTGTGCTGCTCTTCAAGTTGCTAAAAAGCTTGGAAAAGGCAAAAAAGTTCTCGCTGTGCTTCCTAGTA
ACGGAGAGAGATACTTAAGCACGCCTCTTTACCAATTTGAATAG

Upstream 100 bases:

>100_bases
TTGGTTTTATGACGATAAAAAATAAAGATTGACAAAATATTTTGATATTGATAAATTAGATATAATCCCAATAAAATTAC
TCGGAAATTGAGGTGTTCAA

Downstream 100 bases:

>100_bases
GCTCCAGCTGAAACAGGAGGCTGCCGACGATGTCGGCGGCCTTTTGTTTGTCATGGAGCGCAGCCCTTTTAAAAGCTCGG
TGTCCTTGAGTGAACATGCT

Product: hypothetical protein

Products: NA

Alternate protein names: CSase; O-acetylserine (thiol)-lyase; OAS-TL; Superoxide-inducible protein 11; SOI11 [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MVRVANSVYDLIGNTPVVKLNRLVDEDSAEVYVKLEYMNPGSSVKDRIALAMIEDAEEKGLLKEGDTLIEPTSGNTGIGL
AMVAAAKGIKAILVMPDTMSSERRNLLRAYGAELVLTPGAEGMKGAIKKAEELAEEHGYFMPQQFNNPANAEVHRRTTGK
EIAEQFGDGLDAFIAGIGTGGTITGAGEVLKEKNPSIKIFAVEPTDSPVLSGGKPGPHKIQGIGAGFVPKILNTEIYDEI
ITVKNEEAFEFARKAAREEGILGGISSGAAICAALQVAKKLGKGKKVLAVLPSNGERYLSTPLYQFE

Sequences:

>Translated_307_residues
MVRVANSVYDLIGNTPVVKLNRLVDEDSAEVYVKLEYMNPGSSVKDRIALAMIEDAEEKGLLKEGDTLIEPTSGNTGIGL
AMVAAAKGIKAILVMPDTMSSERRNLLRAYGAELVLTPGAEGMKGAIKKAEELAEEHGYFMPQQFNNPANAEVHRRTTGK
EIAEQFGDGLDAFIAGIGTGGTITGAGEVLKEKNPSIKIFAVEPTDSPVLSGGKPGPHKIQGIGAGFVPKILNTEIYDEI
ITVKNEEAFEFARKAAREEGILGGISSGAAICAALQVAKKLGKGKKVLAVLPSNGERYLSTPLYQFE
>Mature_307_residues
MVRVANSVYDLIGNTPVVKLNRLVDEDSAEVYVKLEYMNPGSSVKDRIALAMIEDAEEKGLLKEGDTLIEPTSGNTGIGL
AMVAAAKGIKAILVMPDTMSSERRNLLRAYGAELVLTPGAEGMKGAIKKAEELAEEHGYFMPQQFNNPANAEVHRRTTGK
EIAEQFGDGLDAFIAGIGTGGTITGAGEVLKEKNPSIKIFAVEPTDSPVLSGGKPGPHKIQGIGAGFVPKILNTEIYDEI
ITVKNEEAFEFARKAAREEGILGGISSGAAICAALQVAKKLGKGKKVLAVLPSNGERYLSTPLYQFE

Specific function: Catalyzes the conversion of O-acetylserine to cysteine. Also acts as a sensor of cysteine availability in the signal transduction pathway modulating CymR activity. When cysteine is present, the pool of O-acetylserine (OAS) is low, which leads to the forma

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=303, Percent_Identity=41.2541254125413, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI295821200, Length=303, Percent_Identity=41.2541254125413, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI4557415, Length=303, Percent_Identity=41.2541254125413, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI11345492, Length=255, Percent_Identity=27.4509803921569, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1788754, Length=304, Percent_Identity=53.6184210526316, Blast_Score=276, Evalue=1e-75,
Organism=Escherichia coli, GI2367138, Length=293, Percent_Identity=43.6860068259386, Blast_Score=234, Evalue=4e-63,
Organism=Escherichia coli, GI1790207, Length=275, Percent_Identity=29.0909090909091, Blast_Score=68, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17535051, Length=299, Percent_Identity=52.5083612040134, Blast_Score=299, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI115535073, Length=297, Percent_Identity=55.2188552188552, Blast_Score=288, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI17562970, Length=300, Percent_Identity=49.3333333333333, Blast_Score=275, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI32566674, Length=294, Percent_Identity=49.6598639455782, Blast_Score=269, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI17561720, Length=300, Percent_Identity=47, Blast_Score=235, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI32566672, Length=213, Percent_Identity=49.2957746478873, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17534315, Length=314, Percent_Identity=39.171974522293, Blast_Score=168, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI25147552, Length=312, Percent_Identity=38.1410256410256, Blast_Score=158, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17561716, Length=117, Percent_Identity=44.4444444444444, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6321594, Length=317, Percent_Identity=38.801261829653, Blast_Score=201, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6321449, Length=326, Percent_Identity=35.8895705521472, Blast_Score=135, Evalue=8e-33,
Organism=Drosophila melanogaster, GI24643623, Length=309, Percent_Identity=39.4822006472492, Blast_Score=205, Evalue=3e-53,
Organism=Drosophila melanogaster, GI20129101, Length=309, Percent_Identity=39.4822006472492, Blast_Score=205, Evalue=3e-53,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 32631; Mature: 32631

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRVANSVYDLIGNTPVVKLNRLVDEDSAEVYVKLEYMNPGSSVKDRIALAMIEDAEEKG
CEEEHHHHHHHHCCCCEEEEHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHCHHHCC
LLKEGDTLIEPTSGNTGIGLAMVAAAKGIKAILVMPDTMSSERRNLLRAYGAELVLTPGA
CCCCCCEEEECCCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCCEEECCCC
EGMKGAIKKAEELAEEHGYFMPQQFNNPANAEVHRRTTGKEIAEQFGDGLDAFIAGIGTG
CCHHHHHHHHHHHHHHCCCCCCHHCCCCCCCEEEHHHHHHHHHHHHCCCHHHHHHHCCCC
GTITGAGEVLKEKNPSIKIFAVEPTDSPVLSGGKPGPHKIQGIGAGFVPKILNTEIYDEI
CEECCCHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHH
ITVKNEEAFEFARKAAREEGILGGISSGAAICAALQVAKKLGKGKKVLAVLPSNGERYLS
HHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEC
TPLYQFE
CCCCCCC
>Mature Secondary Structure
MVRVANSVYDLIGNTPVVKLNRLVDEDSAEVYVKLEYMNPGSSVKDRIALAMIEDAEEKG
CEEEHHHHHHHHCCCCEEEEHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHCHHHCC
LLKEGDTLIEPTSGNTGIGLAMVAAAKGIKAILVMPDTMSSERRNLLRAYGAELVLTPGA
CCCCCCEEEECCCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCCEEECCCC
EGMKGAIKKAEELAEEHGYFMPQQFNNPANAEVHRRTTGKEIAEQFGDGLDAFIAGIGTG
CCHHHHHHHHHHHHHHCCCCCCHHCCCCCCCEEEHHHHHHHHHHHHCCCHHHHHHHCCCC
GTITGAGEVLKEKNPSIKIFAVEPTDSPVLSGGKPGPHKIQGIGAGFVPKILNTEIYDEI
CEECCCHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHH
ITVKNEEAFEFARKAAREEGILGGISSGAAICAALQVAKKLGKGKKVLAVLPSNGERYLS
HHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEC
TPLYQFE
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 8755892; 9298659 [H]