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The map label for this gene is metG [H]

Identifier: 52783895

GI number: 52783895

Start: 49915

End: 51903

Strand: Direct

Name: metG [H]

Synonym: BLi00051

Alternate gene names: 52783895

Gene position: 49915-51903 (Clockwise)

Preceding gene: 52783893

Following gene: 52783896

Centisome position: 1.18

GC content: 45.8

Gene sequence:

>1989_bases
ATGCCGCAAGAAAAAAATACGTTTTACATCACTACACCCATTTATTACCCAAGTGGGAAGCTACATATTGGACATGCCTA
CACGACCGTTGCAGGTGATGCGATGGCTCGTTATAAAAGACTGAGAGGCTTTGATGTCAGATACTTAACTGGTACAGATG
AGCACGGACAAAAAATTCAGCAAACGGCGGAGAAGGAAAACATCACTCCGCAGGAATTGGTCGATCGTGCGGCAGAAGAC
ATTCAGCAGCTTTGGAAAAAGCTCGACATATCCAATGACGACTTCATTCGGACGACAGAGGAACGTCATAAAAAAGTGAT
CGAAAAGGTATTTCAGAAGCTGCTTGACAATGGTGACATATATCTTGATGAATATGAGGGCTGGTACAGCATCCCTGACG
AAACGTTTTACACCGAAACCCAGCTCGTTGATGTCGAGCGAAACGAGAAAGGCGAAGTTATCGGAGGGAAAAGTCCTGAC
AGCGGCCACCCCGTTGAGCTAATCAAAGAGGAATCTTACTTCTTCCGCATGGGAAAATACGCGGATCGGCTTCTCGCTTT
CTATGAAGAAAATCCTGAATTCATCCAGCCTGAATCCCGGAAAAATGAAATGATCAACAACTTTATCAAACCGGGTCTTG
AAGATTTGGCTGTTTCCCGGACGACGTTCGACTGGGGGATAAAAGTGCCCGGCAATCCGAAGCATGTCATCTATGTTTGG
ATAGATGCGCTGTTTAATTACATTACGGCACTTGGCTTTAATACGGAAAACGATGAAAATTATCAAAAATATTGGCCGGC
AGACGTTCATCTTGTCGGTAAGGAGATCGTCCGCTTCCATACCATTTACTGGCCGATTATGCTGATGGCATTGGACCTTC
CGCTGCCGAAAAAGGTTTTTGCCCACGGGTGGCTGCTGATGAAAGATGGAAAAATGTCTAAATCAAAAGGCAATGTGGTT
GACCCGGTCACATTAATCGACAGATACGGACTAGATGCATTGCGTTATTATCTTCTCCGCGAAGTGCCGTTCGGTTCTGA
CGGCGTATTTACACCGGAAGGATTTGTCGAGCGGATTAACTATGATTTAGCGAATGATTTAGGCAACCTGCTGAACAGAA
CGGTCGCCATGGTCAACAAATACTTTGACGGCCGGATTCAGTCGTATGAAGGCCCGGTTACCGCTTTTGACGAACCGTTG
TCATCATTCTCGCAAAAGACAATTGAAGCGTACGAGCAGGCGATCGAAAATATGGAGTTTTCCGTTGCGCTTTCATCATT
GTGGCAGTTTGTCAGCAGAACGAATAAATACATCGACGAGACAGCCCCTTGGGTACTGGCAAAAGATAAGGATAAAGAAA
AAGAGCTTCAATCGGTCATGTACCATTTGGCGGAGTCATTGCGCATCACTGCTGTATTGCTGCAGCCGTTCTTAACGCAG
ACGCCTGAAAAGATTTTCGCACAGCTTGGCGTTACGGATGCGTCACTGAAAACCTGGGACAGCATTCAAAGCTTCGGCCA
GCTGAAATCTGTGACAGTTCAAAAAGGAGAACCATTGTTCCCGCGTCTTGAGGCGGAAGACGAAGTCGCTTATATCAAAT
CAAAAATGCAGGGAACGGCTCCAAAAGAAGAACCAAAGCAAGAGGAAAAAGCACATGAACGGCTTCCGGAAATCACGATA
GACGATTTTATGAGCACAGAGCTCAGAGTGGCTGAAGTCATTCATGCTGAACCCGTCAAAAAAGCGGATCGTCTGTTAAA
ACTCCAGCTTGATTTAGGCTTTGAAAAACGCCAAGTCGTTTCAGGAATTGCCAAGCACTACAAACCTGAAGAGCTTGTCG
GCCGGAAAGTCATTTGTGTAACAAATTTAAAACCGGTCAAACTAAGAGGAGAACTTTCTCAAGGCATGATATTGGCTGGA
GAGGACAACGGCGTTCTCTCGCTTGCGGCGGTAGACAGTTCATTAGCAAACGGAACGCGAATCAAATAA

Upstream 100 bases:

>100_bases
TAAAGAGGGATTATATATTGAGTATATACTGAATGAATACACATGCCGCTCTCGTCCTTTGGGCGGGAGTTTTTTGACAT
TTTAATTGGGAGGTTCCATC

Downstream 100 bases:

>100_bases
ATGCAGGCAAAGGGTGTTCCACGTGTAACATTTCGTCGAACACCTTTTGTTTTTTCGACAATTTCTTAAAAAGGTGGCAT
TTTCATGTTATTTGATACAC

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 662; Mature: 661

Protein sequence:

>662_residues
MPQEKNTFYITTPIYYPSGKLHIGHAYTTVAGDAMARYKRLRGFDVRYLTGTDEHGQKIQQTAEKENITPQELVDRAAED
IQQLWKKLDISNDDFIRTTEERHKKVIEKVFQKLLDNGDIYLDEYEGWYSIPDETFYTETQLVDVERNEKGEVIGGKSPD
SGHPVELIKEESYFFRMGKYADRLLAFYEENPEFIQPESRKNEMINNFIKPGLEDLAVSRTTFDWGIKVPGNPKHVIYVW
IDALFNYITALGFNTENDENYQKYWPADVHLVGKEIVRFHTIYWPIMLMALDLPLPKKVFAHGWLLMKDGKMSKSKGNVV
DPVTLIDRYGLDALRYYLLREVPFGSDGVFTPEGFVERINYDLANDLGNLLNRTVAMVNKYFDGRIQSYEGPVTAFDEPL
SSFSQKTIEAYEQAIENMEFSVALSSLWQFVSRTNKYIDETAPWVLAKDKDKEKELQSVMYHLAESLRITAVLLQPFLTQ
TPEKIFAQLGVTDASLKTWDSIQSFGQLKSVTVQKGEPLFPRLEAEDEVAYIKSKMQGTAPKEEPKQEEKAHERLPEITI
DDFMSTELRVAEVIHAEPVKKADRLLKLQLDLGFEKRQVVSGIAKHYKPEELVGRKVICVTNLKPVKLRGELSQGMILAG
EDNGVLSLAAVDSSLANGTRIK

Sequences:

>Translated_662_residues
MPQEKNTFYITTPIYYPSGKLHIGHAYTTVAGDAMARYKRLRGFDVRYLTGTDEHGQKIQQTAEKENITPQELVDRAAED
IQQLWKKLDISNDDFIRTTEERHKKVIEKVFQKLLDNGDIYLDEYEGWYSIPDETFYTETQLVDVERNEKGEVIGGKSPD
SGHPVELIKEESYFFRMGKYADRLLAFYEENPEFIQPESRKNEMINNFIKPGLEDLAVSRTTFDWGIKVPGNPKHVIYVW
IDALFNYITALGFNTENDENYQKYWPADVHLVGKEIVRFHTIYWPIMLMALDLPLPKKVFAHGWLLMKDGKMSKSKGNVV
DPVTLIDRYGLDALRYYLLREVPFGSDGVFTPEGFVERINYDLANDLGNLLNRTVAMVNKYFDGRIQSYEGPVTAFDEPL
SSFSQKTIEAYEQAIENMEFSVALSSLWQFVSRTNKYIDETAPWVLAKDKDKEKELQSVMYHLAESLRITAVLLQPFLTQ
TPEKIFAQLGVTDASLKTWDSIQSFGQLKSVTVQKGEPLFPRLEAEDEVAYIKSKMQGTAPKEEPKQEEKAHERLPEITI
DDFMSTELRVAEVIHAEPVKKADRLLKLQLDLGFEKRQVVSGIAKHYKPEELVGRKVICVTNLKPVKLRGELSQGMILAG
EDNGVLSLAAVDSSLANGTRIK
>Mature_661_residues
PQEKNTFYITTPIYYPSGKLHIGHAYTTVAGDAMARYKRLRGFDVRYLTGTDEHGQKIQQTAEKENITPQELVDRAAEDI
QQLWKKLDISNDDFIRTTEERHKKVIEKVFQKLLDNGDIYLDEYEGWYSIPDETFYTETQLVDVERNEKGEVIGGKSPDS
GHPVELIKEESYFFRMGKYADRLLAFYEENPEFIQPESRKNEMINNFIKPGLEDLAVSRTTFDWGIKVPGNPKHVIYVWI
DALFNYITALGFNTENDENYQKYWPADVHLVGKEIVRFHTIYWPIMLMALDLPLPKKVFAHGWLLMKDGKMSKSKGNVVD
PVTLIDRYGLDALRYYLLREVPFGSDGVFTPEGFVERINYDLANDLGNLLNRTVAMVNKYFDGRIQSYEGPVTAFDEPLS
SFSQKTIEAYEQAIENMEFSVALSSLWQFVSRTNKYIDETAPWVLAKDKDKEKELQSVMYHLAESLRITAVLLQPFLTQT
PEKIFAQLGVTDASLKTWDSIQSFGQLKSVTVQKGEPLFPRLEAEDEVAYIKSKMQGTAPKEEPKQEEKAHERLPEITID
DFMSTELRVAEVIHAEPVKKADRLLKLQLDLGFEKRQVVSGIAKHYKPEELVGRKVICVTNLKPVKLRGELSQGMILAGE
DNGVLSLAAVDSSLANGTRIK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=549, Percent_Identity=35.8834244080146, Blast_Score=324, Evalue=1e-88,
Organism=Homo sapiens, GI14043022, Length=667, Percent_Identity=25.0374812593703, Blast_Score=169, Evalue=1e-41,
Organism=Escherichia coli, GI1788432, Length=699, Percent_Identity=29.3276108726753, Blast_Score=300, Evalue=2e-82,
Organism=Escherichia coli, GI1789455, Length=102, Percent_Identity=34.3137254901961, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI212645096, Length=493, Percent_Identity=35.2941176470588, Blast_Score=299, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI71992781, Length=383, Percent_Identity=34.7258485639687, Blast_Score=214, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI17540794, Length=603, Percent_Identity=26.0364842454395, Blast_Score=154, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI71997510, Length=178, Percent_Identity=30.3370786516854, Blast_Score=66, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI71997517, Length=178, Percent_Identity=30.3370786516854, Blast_Score=66, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6321610, Length=527, Percent_Identity=31.1195445920304, Blast_Score=270, Evalue=4e-73,
Organism=Saccharomyces cerevisiae, GI6321704, Length=566, Percent_Identity=25.2650176678445, Blast_Score=138, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6323414, Length=225, Percent_Identity=28.8888888888889, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI21358483, Length=561, Percent_Identity=32.0855614973262, Blast_Score=293, Evalue=3e-79,
Organism=Drosophila melanogaster, GI24655311, Length=565, Percent_Identity=27.0796460176991, Blast_Score=171, Evalue=1e-42,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 75795; Mature: 75664

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQEKNTFYITTPIYYPSGKLHIGHAYTTVAGDAMARYKRLRGFDVRYLTGTDEHGQKIQ
CCCCCCEEEEECCEEECCCCEEECCHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHH
QTAEKENITPQELVDRAAEDIQQLWKKLDISNDDFIRTTEERHKKVIEKVFQKLLDNGDI
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEECHHHHHHHHHHHHHHHHHCCCCE
YLDEYEGWYSIPDETFYTETQLVDVERNEKGEVIGGKSPDSGHPVELIKEESYFFRMGKY
EEECCCCEECCCCCCEECCEEEEECCCCCCCCEECCCCCCCCCCHHHHHCCHHHHHHHHH
ADRLLAFYEENPEFIQPESRKNEMINNFIKPGLEDLAVSRTTFDWGIKVPGNPKHVIYVW
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHCCEEEECCEECCCCCCEEEEEE
IDALFNYITALGFNTENDENYQKYWPADVHLVGKEIVRFHTIYWPIMLMALDLPLPKKVF
HHHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
AHGWLLMKDGKMSKSKGNVVDPVTLIDRYGLDALRYYLLREVPFGSDGVFTPEGFVERIN
HCCEEEEECCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHC
YDLANDLGNLLNRTVAMVNKYFDGRIQSYEGPVTAFDEPLSSFSQKTIEAYEQAIENMEF
HHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHH
SVALSSLWQFVSRTNKYIDETAPWVLAKDKDKEKELQSVMYHLAESLRITAVLLQPFLTQ
HHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TPEKIFAQLGVTDASLKTWDSIQSFGQLKSVTVQKGEPLFPRLEAEDEVAYIKSKMQGTA
CHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHCCCC
PKEEPKQEEKAHERLPEITIDDFMSTELRVAEVIHAEPVKKADRLLKLQLDLGFEKRQVV
CCCCCHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHCHHHHHHHEEEEEECCCCHHHHHH
SGIAKHYKPEELVGRKVICVTNLKPVKLRGELSQGMILAGEDNGVLSLAAVDSSLANGTR
HHHHHHCCCHHHHCCEEEEEECCCCEEEECCCCCCEEEEECCCCEEEEEECCHHHCCCCC
IK
CC
>Mature Secondary Structure 
PQEKNTFYITTPIYYPSGKLHIGHAYTTVAGDAMARYKRLRGFDVRYLTGTDEHGQKIQ
CCCCCEEEEECCEEECCCCEEECCHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHH
QTAEKENITPQELVDRAAEDIQQLWKKLDISNDDFIRTTEERHKKVIEKVFQKLLDNGDI
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEECHHHHHHHHHHHHHHHHHCCCCE
YLDEYEGWYSIPDETFYTETQLVDVERNEKGEVIGGKSPDSGHPVELIKEESYFFRMGKY
EEECCCCEECCCCCCEECCEEEEECCCCCCCCEECCCCCCCCCCHHHHHCCHHHHHHHHH
ADRLLAFYEENPEFIQPESRKNEMINNFIKPGLEDLAVSRTTFDWGIKVPGNPKHVIYVW
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHCCEEEECCEECCCCCCEEEEEE
IDALFNYITALGFNTENDENYQKYWPADVHLVGKEIVRFHTIYWPIMLMALDLPLPKKVF
HHHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
AHGWLLMKDGKMSKSKGNVVDPVTLIDRYGLDALRYYLLREVPFGSDGVFTPEGFVERIN
HCCEEEEECCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHC
YDLANDLGNLLNRTVAMVNKYFDGRIQSYEGPVTAFDEPLSSFSQKTIEAYEQAIENMEF
HHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHH
SVALSSLWQFVSRTNKYIDETAPWVLAKDKDKEKELQSVMYHLAESLRITAVLLQPFLTQ
HHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TPEKIFAQLGVTDASLKTWDSIQSFGQLKSVTVQKGEPLFPRLEAEDEVAYIKSKMQGTA
CHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHCCCC
PKEEPKQEEKAHERLPEITIDDFMSTELRVAEVIHAEPVKKADRLLKLQLDLGFEKRQVV
CCCCCHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHCHHHHHHHEEEEEECCCCHHHHHH
SGIAKHYKPEELVGRKVICVTNLKPVKLRGELSQGMILAGEDNGVLSLAAVDSSLANGTR
HHHHHHCCCHHHHCCEEEEEECCCCEEEECCCCCCEEEEECCCCEEEEEECCHHHCCCCC
IK
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]