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The map label for this gene is dacA [H]

Identifier: 52783865

GI number: 52783865

Start: 17505

End: 18830

Strand: Direct

Name: dacA [H]

Synonym: BLi00015

Alternate gene names: 52783865

Gene position: 17505-18830 (Clockwise)

Preceding gene: 52783864

Following gene: 52783866

Centisome position: 0.41

GC content: 43.97

Gene sequence:

>1326_bases
TTGAAAAGCAAGAGATTAAAGCAGCTGATCATGTTAATCGTTGCTTTTGCCGTGACTGTAGGTGCATTTTCTCCTATGTC
AACTGCAAAAGCTGCAAATGATCCAATCAATGTAGACGCGAAAGCAGCAATCCTGGTGGAAGCTTCATCGGGGAAAATTT
TGTACAGTAAAAATGCCGACCAGCGTCTTCCTGTTGCCAGCATGGCAAAAATGATGACGGAGTATCTTCTGTTGGAAGCG
ATTCACGAAGGCAAAGTGAAATGGGATCAGAAGTACACGCCTGACGATTATGTATACGAGATTTCCCAAGATCGTTCACT
ATCCAATGTTCCTCTTCGTAAAGACGGATCATACACTGTAAAAGAGCTTTATCAGGCGACTGCAATTTACTCTGCAAACG
CGGCGGCTATTGCACTTTCTGAAATTGTTGCCGGTTCTGAATCGAAGTTTGTAGAGTTAATGAACAAAAAAGCCAAAGAG
CTTGGAATGAAAGATTTCAAATTTGTGAATGCCACAGGTTTGGAAAACAAGGATCTTCACGGCAAACATCCGAGCGGAAC
AAATGCCAATGAAGAAAATGAAGTGTCTGCGAGAGACATGGCGCTCCTTGCCGATCATTTAGTGAGCGATTATCCAGAGA
TCCTCGATACGGCGAGCATTGCCAAAACGAAGTTCAGAAAAGGCACAGACGATGAAATGGATATGCCGAACTGGAACTTT
ATGCTGAAAGGCCTTGTTCAGCAATATGAAGGTGTGGACGGGCTGAAAACCGGGTCAACCGACTCAGCCGGATCATGCTT
TACGGCTACTGCTGAAAGAAACGGCATGAGAGTGATTTCCGTCGTTCTGAATGCGAAAGGCGACCTTCATACGGCGCGAT
TTAAAGAAACGAAAAAAATGCTCGATTATGCATTTGAAAACTTCACTATGAAAGAGTTTTACGCAAAAGGCGCACAAGTG
AAAGGCAACGAAACAGTCACAGTCGACAAAGGGAAAGAAACTGAAGTCGGCATCGTCACAGAAAAAGCCTTTTCAATGCC
TGTGAAGAACGGTGAAGAAAAGAATTACAAAGCGAAAGTGACGCTGAACAAAGACGAGCTGACCGCACCTGTTAAAAAAG
GTGAGAAAGTCGGTACCTTGACGGCCTCCTATAAGGGAGAAGAAAAGGACTACGGATTTCTCGGCAGCGATGTATCAGGT
GTAAACCTGGTGACAAAAGAAGATGATGAGAAAGCAAACTGGTTTATTTTAACGATGAGAAGCATCGGCGGCTTTTTTGC
CGGTATATGGAACAGCATTGTTGAAATGGTAACCGGCTGGTTTTAA

Upstream 100 bases:

>100_bases
TTGCTTCATTATGATAAAATTTATAATGTTGTGTTTGAAAAATGTCTTAAAAGGCTTTGATATAGATTATTAGTCTGATG
AAAAGCGGAGGTATCACGAT

Downstream 100 bases:

>100_bases
AAAATAAAGAGCTCTATTTGATAGGGCTCTTTATTGTCATATTCAGATAGAATTACCATTTACAATCCTAACGATTTGTT
ATTAAAATAGAAATAATCTT

Product: DacA

Products: NA

Alternate protein names: CPase; DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MKSKRLKQLIMLIVAFAVTVGAFSPMSTAKAANDPINVDAKAAILVEASSGKILYSKNADQRLPVASMAKMMTEYLLLEA
IHEGKVKWDQKYTPDDYVYEISQDRSLSNVPLRKDGSYTVKELYQATAIYSANAAAIALSEIVAGSESKFVELMNKKAKE
LGMKDFKFVNATGLENKDLHGKHPSGTNANEENEVSARDMALLADHLVSDYPEILDTASIAKTKFRKGTDDEMDMPNWNF
MLKGLVQQYEGVDGLKTGSTDSAGSCFTATAERNGMRVISVVLNAKGDLHTARFKETKKMLDYAFENFTMKEFYAKGAQV
KGNETVTVDKGKETEVGIVTEKAFSMPVKNGEEKNYKAKVTLNKDELTAPVKKGEKVGTLTASYKGEEKDYGFLGSDVSG
VNLVTKEDDEKANWFILTMRSIGGFFAGIWNSIVEMVTGWF

Sequences:

>Translated_441_residues
MKSKRLKQLIMLIVAFAVTVGAFSPMSTAKAANDPINVDAKAAILVEASSGKILYSKNADQRLPVASMAKMMTEYLLLEA
IHEGKVKWDQKYTPDDYVYEISQDRSLSNVPLRKDGSYTVKELYQATAIYSANAAAIALSEIVAGSESKFVELMNKKAKE
LGMKDFKFVNATGLENKDLHGKHPSGTNANEENEVSARDMALLADHLVSDYPEILDTASIAKTKFRKGTDDEMDMPNWNF
MLKGLVQQYEGVDGLKTGSTDSAGSCFTATAERNGMRVISVVLNAKGDLHTARFKETKKMLDYAFENFTMKEFYAKGAQV
KGNETVTVDKGKETEVGIVTEKAFSMPVKNGEEKNYKAKVTLNKDELTAPVKKGEKVGTLTASYKGEEKDYGFLGSDVSG
VNLVTKEDDEKANWFILTMRSIGGFFAGIWNSIVEMVTGWF
>Mature_441_residues
MKSKRLKQLIMLIVAFAVTVGAFSPMSTAKAANDPINVDAKAAILVEASSGKILYSKNADQRLPVASMAKMMTEYLLLEA
IHEGKVKWDQKYTPDDYVYEISQDRSLSNVPLRKDGSYTVKELYQATAIYSANAAAIALSEIVAGSESKFVELMNKKAKE
LGMKDFKFVNATGLENKDLHGKHPSGTNANEENEVSARDMALLADHLVSDYPEILDTASIAKTKFRKGTDDEMDMPNWNF
MLKGLVQQYEGVDGLKTGSTDSAGSCFTATAERNGMRVISVVLNAKGDLHTARFKETKKMLDYAFENFTMKEFYAKGAQV
KGNETVTVDKGKETEVGIVTEKAFSMPVKNGEEKNYKAKVTLNKDELTAPVKKGEKVGTLTASYKGEEKDYGFLGSDVSG
VNLVTKEDDEKANWFILTMRSIGGFFAGIWNSIVEMVTGWF

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=427, Percent_Identity=33.2552693208431, Blast_Score=178, Evalue=5e-46,
Organism=Escherichia coli, GI87082022, Length=418, Percent_Identity=27.7511961722488, Blast_Score=138, Evalue=7e-34,
Organism=Escherichia coli, GI1786851, Length=413, Percent_Identity=30.5084745762712, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI87082054, Length=260, Percent_Identity=27.6923076923077, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 48581; Mature: 48581

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSKRLKQLIMLIVAFAVTVGAFSPMSTAKAANDPINVDAKAAILVEASSGKILYSKNAD
CCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCEEEEEEEECCCCEEEECCCC
QRLPVASMAKMMTEYLLLEAIHEGKVKWDQKYTPDDYVYEISQDRSLSNVPLRKDGSYTV
CCCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCHHEEEECCCCCCCCCCCCCCCCHHH
KELYQATAIYSANAAAIALSEIVAGSESKFVELMNKKAKELGMKDFKFVNATGLENKDLH
HHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCC
GKHPSGTNANEENEVSARDMALLADHLVSDYPEILDTASIAKTKFRKGTDDEMDMPNWNF
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
MLKGLVQQYEGVDGLKTGSTDSAGSCFTATAERNGMRVISVVLNAKGDLHTARFKETKKM
HHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHH
LDYAFENFTMKEFYAKGAQVKGNETVTVDKGKETEVGIVTEKAFSMPVKNGEEKNYKAKV
HHHHHHCCHHHHHHHCCCEECCCCEEEEECCCCCEEEEEEHHHHCCCCCCCCCCCEEEEE
TLNKDELTAPVKKGEKVGTLTASYKGEEKDYGFLGSDVSGVNLVTKEDDEKANWFILTMR
EECHHHHHCHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEHH
SIGGFFAGIWNSIVEMVTGWF
HHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKSKRLKQLIMLIVAFAVTVGAFSPMSTAKAANDPINVDAKAAILVEASSGKILYSKNAD
CCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCEEEEEEEECCCCEEEECCCC
QRLPVASMAKMMTEYLLLEAIHEGKVKWDQKYTPDDYVYEISQDRSLSNVPLRKDGSYTV
CCCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCHHEEEECCCCCCCCCCCCCCCCHHH
KELYQATAIYSANAAAIALSEIVAGSESKFVELMNKKAKELGMKDFKFVNATGLENKDLH
HHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCC
GKHPSGTNANEENEVSARDMALLADHLVSDYPEILDTASIAKTKFRKGTDDEMDMPNWNF
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
MLKGLVQQYEGVDGLKTGSTDSAGSCFTATAERNGMRVISVVLNAKGDLHTARFKETKKM
HHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHH
LDYAFENFTMKEFYAKGAQVKGNETVTVDKGKETEVGIVTEKAFSMPVKNGEEKNYKAKV
HHHHHHCCHHHHHHHCCCEECCCCEEEEECCCCCEEEEEEHHHHCCCCCCCCCCCEEEEE
TLNKDELTAPVKKGEKVGTLTASYKGEEKDYGFLGSDVSGVNLVTKEDDEKANWFILTMR
EECHHHHHCHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEHH
SIGGFFAGIWNSIVEMVTGWF
HHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 6768745; 3087956; 6780559; 111240 [H]