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The map label for this gene is gatA

Identifier: 51893959

GI number: 51893959

Start: 3035926

End: 3037392

Strand: Reverse

Name: gatA

Synonym: STH2821

Alternate gene names: 51893959

Gene position: 3037392-3035926 (Counterclockwise)

Preceding gene: 51893960

Following gene: 51893958

Centisome position: 85.17

GC content: 70.07

Gene sequence:

>1467_bases
ATGCTGAGCGCGGCCCGGCTCAACCGGCTCTTTCTGGCCGGGGAGCTGTCGGCGGTGGAAATTGCGGAATCTGCCCTGAG
CCGCATCGCACAGGTTGAACCCGCGGTGGGCGCGTTCATCACGGTGGCGGCCGATCACGTGATCGAGCGGGCGAAGAAGC
TGGACGCGCGCCGCAAGGCCGGCGACACGGAGCTGGGCCCGCTGGCCGGCGTGCCGATCGCGGTGAAGGATAACATCTGC
ACCAGCGGGATGGAGACCACCTGCGCCTCCCGCATCCTGAAGGGGTACGTCTCGCCCTTCGACGCGACGGTGGTGGAGCG
GCTGCGTGCCGCCGGCGCGATGATCATCGGCAAGGCGAACATGGACGAGTTCGCGATGGGCTCCTCGGGCGAATCCTCTG
CGTTCGGCGTGACCCGCAACCCGTGGGACCTGGAGCGCGTGCCCGGCGGCTCATCCTCCGGGTCGGCCGCGGCGGTCGCG
GCCGGGGAGGCCCCTCTCGCCCTGGGCACGGACACGGGCGGCTCCATCCGGCAGCCGGCCGCATTCACCGGCATCGTCGG
GCTGAAGCCCACCTACGGGTACGTCAGCCGCTACGGCGTGGTCGCGTTCGCCTCGTCGCTGGACCAGGTGGGTCCGATGG
GCCGCGATGTGGAGGACGTGGCCCGGCTCTTCGAGGTGATCGCCGGACCCGACCGGCGCGACGCGACCAACGCCGGGCGC
ACCCCGCCGGCGCTGAAGTTCGGCGGCGAACCGTCCCTCAGCGGCGTGCGCCTCGGCGTGCCGAAGGAGCTGCTCGGCCC
CGGCATCGACCCCGGTGTGAAGGCCCGGGTCGAGGAGGCCATTGCCCAGTTGGAGGAGCTCGGCGCGACCGTGGAGGAGT
GCTCGCTGCCCTCCACCGAGTACGCGCTGTCGGCGTACTACGTCATCGCGGTCGCCGAGGCCTCTTCAAACCTGGCCCGG
TTCGACGGCGTGCGGTACGGCTACCGCGCCGCCCAGGCCGGCGGGTTGCACGAGATGTACAGCAAGACCCGCGGCGAGGG
CTTCGGCACGGAAGTGAAGCGCCGCATCATGCTGGGCACCTACGTCCTCTCCGCAGGCCATTACGACGCCTACTACCGGC
GGGCGCAGCAGGTACGCACCCTGGTCGTGCGCGACTTCGAGCGCGCCTTTGAGCGGTACGACGCCCTGGTGACCCCCACC
ACGCCGTTCACCGCCTGGAAGATCGGGGAGAAGGTCGACGACCCGGTCTCCATGTACCTGGGCGATATCTGCACCATTCC
GGTCAACCTGGCGGGGCTGCCGGCCGTCTCCGTCCCCTGCGGGTTCGTGGACGGCCTGCCGGTCGGGATGCAGCTGATCG
GCAAGCCCTTTGCCGACACCCAGATCCTGCAGATCGCCTGGGCGTACCAGAAGGTGACCAAGCATCACGAGGCCCGTCCG
GCCCTGACTGAGGAAGGAGGTCGGTAG

Upstream 100 bases:

>100_bases
GGACCGGGAGGCGGTTCTGGCGCTGGCCCCGGAGGCGGAGGCCGGCTGTTACAAGGTGCCGAAGATTACGGAGGGCTAAG
GGATGTCTGGGATGACGCAC

Downstream 100 bases:

>100_bases
CCGTGTCCACCGCCAAGTGGGAGACCGTTATCGGCCTCGAGGTCCACGTGGAGCTGTCCACTGAGACCAAGATCTGGTGC
GGTTGCAAGAACGAGTTCGG

Product: glutamyl-tRNA(Gln) amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MLSAARLNRLFLAGELSAVEIAESALSRIAQVEPAVGAFITVAADHVIERAKKLDARRKAGDTELGPLAGVPIAVKDNIC
TSGMETTCASRILKGYVSPFDATVVERLRAAGAMIIGKANMDEFAMGSSGESSAFGVTRNPWDLERVPGGSSSGSAAAVA
AGEAPLALGTDTGGSIRQPAAFTGIVGLKPTYGYVSRYGVVAFASSLDQVGPMGRDVEDVARLFEVIAGPDRRDATNAGR
TPPALKFGGEPSLSGVRLGVPKELLGPGIDPGVKARVEEAIAQLEELGATVEECSLPSTEYALSAYYVIAVAEASSNLAR
FDGVRYGYRAAQAGGLHEMYSKTRGEGFGTEVKRRIMLGTYVLSAGHYDAYYRRAQQVRTLVVRDFERAFERYDALVTPT
TPFTAWKIGEKVDDPVSMYLGDICTIPVNLAGLPAVSVPCGFVDGLPVGMQLIGKPFADTQILQIAWAYQKVTKHHEARP
ALTEEGGR

Sequences:

>Translated_488_residues
MLSAARLNRLFLAGELSAVEIAESALSRIAQVEPAVGAFITVAADHVIERAKKLDARRKAGDTELGPLAGVPIAVKDNIC
TSGMETTCASRILKGYVSPFDATVVERLRAAGAMIIGKANMDEFAMGSSGESSAFGVTRNPWDLERVPGGSSSGSAAAVA
AGEAPLALGTDTGGSIRQPAAFTGIVGLKPTYGYVSRYGVVAFASSLDQVGPMGRDVEDVARLFEVIAGPDRRDATNAGR
TPPALKFGGEPSLSGVRLGVPKELLGPGIDPGVKARVEEAIAQLEELGATVEECSLPSTEYALSAYYVIAVAEASSNLAR
FDGVRYGYRAAQAGGLHEMYSKTRGEGFGTEVKRRIMLGTYVLSAGHYDAYYRRAQQVRTLVVRDFERAFERYDALVTPT
TPFTAWKIGEKVDDPVSMYLGDICTIPVNLAGLPAVSVPCGFVDGLPVGMQLIGKPFADTQILQIAWAYQKVTKHHEARP
ALTEEGGR
>Mature_488_residues
MLSAARLNRLFLAGELSAVEIAESALSRIAQVEPAVGAFITVAADHVIERAKKLDARRKAGDTELGPLAGVPIAVKDNIC
TSGMETTCASRILKGYVSPFDATVVERLRAAGAMIIGKANMDEFAMGSSGESSAFGVTRNPWDLERVPGGSSSGSAAAVA
AGEAPLALGTDTGGSIRQPAAFTGIVGLKPTYGYVSRYGVVAFASSLDQVGPMGRDVEDVARLFEVIAGPDRRDATNAGR
TPPALKFGGEPSLSGVRLGVPKELLGPGIDPGVKARVEEAIAQLEELGATVEECSLPSTEYALSAYYVIAVAEASSNLAR
FDGVRYGYRAAQAGGLHEMYSKTRGEGFGTEVKRRIMLGTYVLSAGHYDAYYRRAQQVRTLVVRDFERAFERYDALVTPT
TPFTAWKIGEKVDDPVSMYLGDICTIPVNLAGLPAVSVPCGFVDGLPVGMQLIGKPFADTQILQIAWAYQKVTKHHEARP
ALTEEGGR

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=485, Percent_Identity=40.4123711340206, Blast_Score=338, Evalue=5e-93,
Organism=Homo sapiens, GI195972892, Length=491, Percent_Identity=25.6619144602851, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI166795287, Length=157, Percent_Identity=38.2165605095541, Blast_Score=78, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=422, Percent_Identity=39.5734597156398, Blast_Score=284, Evalue=8e-77,
Organism=Caenorhabditis elegans, GI17537465, Length=491, Percent_Identity=25.6619144602851, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17556264, Length=302, Percent_Identity=27.1523178807947, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17538252, Length=205, Percent_Identity=30.7317073170732, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71990152, Length=306, Percent_Identity=26.797385620915, Blast_Score=85, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=128, Percent_Identity=38.28125, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17556278, Length=128, Percent_Identity=38.28125, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17538254, Length=179, Percent_Identity=31.2849162011173, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=424, Percent_Identity=36.0849056603774, Blast_Score=252, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6319685, Length=461, Percent_Identity=34.4902386117137, Blast_Score=165, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6320448, Length=511, Percent_Identity=23.4833659491194, Blast_Score=97, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24648113, Length=488, Percent_Identity=39.344262295082, Blast_Score=313, Evalue=2e-85,
Organism=Drosophila melanogaster, GI24652985, Length=488, Percent_Identity=28.0737704918033, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI19922090, Length=488, Percent_Identity=28.0737704918033, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24652981, Length=488, Percent_Identity=28.0737704918033, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24652983, Length=488, Percent_Identity=28.0737704918033, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24644968, Length=487, Percent_Identity=25.8726899383984, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24648435, Length=495, Percent_Identity=26.0606060606061, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24648437, Length=495, Percent_Identity=26.0606060606061, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI45550774, Length=495, Percent_Identity=26.0606060606061, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI161078093, Length=506, Percent_Identity=27.0750988142292, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24648441, Length=436, Percent_Identity=26.605504587156, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648439, Length=436, Percent_Identity=26.605504587156, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21356731, Length=222, Percent_Identity=31.981981981982, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_SYMTH (Q67KJ2)

Other databases:

- EMBL:   AP006840
- RefSeq:   YP_076650.1
- ProteinModelPortal:   Q67KJ2
- SMR:   Q67KJ2
- GeneID:   2980426
- GenomeReviews:   AP006840_GR
- KEGG:   sth:STH2821
- NMPDR:   fig|292459.1.peg.2696
- HOGENOM:   HBG481888
- OMA:   TESSCYG
- BioCyc:   STHE292459:STH2821-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 51669; Mature: 51669

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 76-76 ACT_SITE 151-151 ACT_SITE 175-175

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSAARLNRLFLAGELSAVEIAESALSRIAQVEPAVGAFITVAADHVIERAKKLDARRKA
CCCHHHHHHHHEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GDTELGPLAGVPIAVKDNICTSGMETTCASRILKGYVSPFDATVVERLRAAGAMIIGKAN
CCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEEECCC
MDEFAMGSSGESSAFGVTRNPWDLERVPGGSSSGSAAAVAAGEAPLALGTDTGGSIRQPA
CCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEECCCCCCCCCCC
AFTGIVGLKPTYGYVSRYGVVAFASSLDQVGPMGRDVEDVARLFEVIAGPDRRDATNAGR
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
TPPALKFGGEPSLSGVRLGVPKELLGPGIDPGVKARVEEAIAQLEELGATVEECSLPSTE
CCCCCCCCCCCCCCCEEECCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCHH
YALSAYYVIAVAEASSNLARFDGVRYGYRAAQAGGLHEMYSKTRGEGFGTEVKRRIMLGT
HHHHEEEEEEEECCCCCHHHHCCCHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHH
YVLSAGHYDAYYRRAQQVRTLVVRDFERAFERYDALVTPTTPFTAWKIGEKVDDPVSMYL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCHHHHCCCCCCCHHHHHH
GDICTIPVNLAGLPAVSVPCGFVDGLPVGMQLIGKPFADTQILQIAWAYQKVTKHHEARP
CCHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCC
ALTEEGGR
CCCCCCCC
>Mature Secondary Structure
MLSAARLNRLFLAGELSAVEIAESALSRIAQVEPAVGAFITVAADHVIERAKKLDARRKA
CCCHHHHHHHHEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GDTELGPLAGVPIAVKDNICTSGMETTCASRILKGYVSPFDATVVERLRAAGAMIIGKAN
CCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEEECCC
MDEFAMGSSGESSAFGVTRNPWDLERVPGGSSSGSAAAVAAGEAPLALGTDTGGSIRQPA
CCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEECCCCCCCCCCC
AFTGIVGLKPTYGYVSRYGVVAFASSLDQVGPMGRDVEDVARLFEVIAGPDRRDATNAGR
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
TPPALKFGGEPSLSGVRLGVPKELLGPGIDPGVKARVEEAIAQLEELGATVEECSLPSTE
CCCCCCCCCCCCCCCEEECCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCHH
YALSAYYVIAVAEASSNLARFDGVRYGYRAAQAGGLHEMYSKTRGEGFGTEVKRRIMLGT
HHHHEEEEEEEECCCCCHHHHCCCHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHH
YVLSAGHYDAYYRRAQQVRTLVVRDFERAFERYDALVTPTTPFTAWKIGEKVDDPVSMYL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCHHHHCCCCCCCHHHHHH
GDICTIPVNLAGLPAVSVPCGFVDGLPVGMQLIGKPFADTQILQIAWAYQKVTKHHEARP
CCHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCC
ALTEEGGR
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA