Click here to switch to the map view.

The map label for this gene is gatB

Identifier: 51893958

GI number: 51893958

Start: 3034451

End: 3035923

Strand: Reverse

Name: gatB

Synonym: STH2820

Alternate gene names: 51893958

Gene position: 3035923-3034451 (Counterclockwise)

Preceding gene: 51893959

Following gene: 51893957

Centisome position: 85.13

GC content: 66.12

Gene sequence:

>1473_bases
GTGTCCACCGCCAAGTGGGAGACCGTTATCGGCCTCGAGGTCCACGTGGAGCTGTCCACTGAGACCAAGATCTGGTGCGG
TTGCAAGAACGAGTTCGGGGCCGAGCCGAACACCAACGTCTGCCCGGTGTGCCTCGCCCTGCCCGGCGCGCTGCCGGTCC
TGAATTACAAGGCCGTGGAGTACACCATCCGCGCGGGACTGGCCCTGAACTGCAAGATCCAGCGGCACTCCAAGTTCGAC
CGGAAGAACTACTTCTACGCCGATCTGCCCTCGGGCTACCAGATCAGCCAATTCGACCTGCCGCTGTGCTACGACGGCTA
CGTGGACATCACGAAGAAGGACGGTACCACCCGCCGCATCCGCATCAAGCGGATCCACCTGGAGACCGACGCGGGGAAGC
TGCTCCATGCCGGCGACGACGTCGCGGCGGCGGATTACTCGCTGGTCGACTTCAACCGCGCCGGCGTGCCGCTCATCGAG
ATCGTCACCGAACCGGACCTGCGCTCGGCCGAGGAGGCCGGCCTGTTCCTGCAGAAGCTGCGCACGATCCTGAAGTACTC
GGGCGTCAGCGACGTGAAGATGGAAGAGGGCTCCATGCGCTGCGACGTGAACCTGTCGGTGCGCCCGGCGGGGTCCAGCG
AGTACGGCGTGCGGACGGAGCTGAAGAACGTCAACTCCTTCTCGGCCGTCATGCGGGGCATCGAGTACGAGGAGAAGCGG
CATATCCGTATCCTGGAGGAGGGCGGCCAGCCCGAGCAGGAGACCCGTTCCTGGCGCGACGCCCAGGGCATCTCGGTGCT
GCTGCGCTCCAAGGAGGACGCCGAGGACTACCGCTACTTCCCCGAGCCCGACCTGCCGCCGCTGGAGGTGTCGGCCGAGG
AGATCGAACGCATCCGGGCCGGCCTGCCCGAGCTGCCCGACGCCCTCATGCAGCGGCTGATGACCGAGTACGGCCTCTCC
GCTTACGACGCCTCCGTCATCGTCGCCGAGCGGGAGTACGCCCAGTGGTTCCTGCACGCGGTGGAGCTGGCCGGCGCCGG
CCAGGCCAAGACCGTCGCCAACTGGCAGATCAACGAGTTGTACCGGGTGATGAACGAGAAGGGGCTCGGCCCCGAACAGA
TCCCCGTCACGCCCGAGCAGCTGGTGGGCATGCTGAAGCTGATCGAGCAGGGAACCATCACCGGCAAGATCGCCAAGACC
GTCTTCGACAAGATGGTGGAGACGGGCAAGGACGCCGAGACCATCGTCAAGGAAGAGGGGCTCACCCAGGTGGCCGATGA
GGGCGAGCTGCTCGCCATCGCCCGGGAGGTGGTGGCCTCCAACCCGAAGGTCTTCGAGGACTGGAAGGCCGGCAAGCAGT
CGGCCGCCCAGTGGTTCGTCGGCCAGATCATGAAGCGGACCCGTGGGCGGGCCAACCCGCAGATGGCCCTGAAGCTGGTG
ACCCAGGCGCTCGAAGAGCAGGCGCAGAAGTAA

Upstream 100 bases:

>100_bases
TTGCCGACACCCAGATCCTGCAGATCGCCTGGGCGTACCAGAAGGTGACCAAGCATCACGAGGCCCGTCCGGCCCTGACT
GAGGAAGGAGGTCGGTAGCC

Downstream 100 bases:

>100_bases
GGACGCCCCCCGCCGTAGGAACTGCGGCGGGGGCGTCTAATCTTTTCGGTAAAGATTGAAAGGAGGCGTGGCCCGTGGAC
CTGGTAACCGGCGCCACCGG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MSTAKWETVIGLEVHVELSTETKIWCGCKNEFGAEPNTNVCPVCLALPGALPVLNYKAVEYTIRAGLALNCKIQRHSKFD
RKNYFYADLPSGYQISQFDLPLCYDGYVDITKKDGTTRRIRIKRIHLETDAGKLLHAGDDVAAADYSLVDFNRAGVPLIE
IVTEPDLRSAEEAGLFLQKLRTILKYSGVSDVKMEEGSMRCDVNLSVRPAGSSEYGVRTELKNVNSFSAVMRGIEYEEKR
HIRILEEGGQPEQETRSWRDAQGISVLLRSKEDAEDYRYFPEPDLPPLEVSAEEIERIRAGLPELPDALMQRLMTEYGLS
AYDASVIVAEREYAQWFLHAVELAGAGQAKTVANWQINELYRVMNEKGLGPEQIPVTPEQLVGMLKLIEQGTITGKIAKT
VFDKMVETGKDAETIVKEEGLTQVADEGELLAIAREVVASNPKVFEDWKAGKQSAAQWFVGQIMKRTRGRANPQMALKLV
TQALEEQAQK

Sequences:

>Translated_490_residues
MSTAKWETVIGLEVHVELSTETKIWCGCKNEFGAEPNTNVCPVCLALPGALPVLNYKAVEYTIRAGLALNCKIQRHSKFD
RKNYFYADLPSGYQISQFDLPLCYDGYVDITKKDGTTRRIRIKRIHLETDAGKLLHAGDDVAAADYSLVDFNRAGVPLIE
IVTEPDLRSAEEAGLFLQKLRTILKYSGVSDVKMEEGSMRCDVNLSVRPAGSSEYGVRTELKNVNSFSAVMRGIEYEEKR
HIRILEEGGQPEQETRSWRDAQGISVLLRSKEDAEDYRYFPEPDLPPLEVSAEEIERIRAGLPELPDALMQRLMTEYGLS
AYDASVIVAEREYAQWFLHAVELAGAGQAKTVANWQINELYRVMNEKGLGPEQIPVTPEQLVGMLKLIEQGTITGKIAKT
VFDKMVETGKDAETIVKEEGLTQVADEGELLAIAREVVASNPKVFEDWKAGKQSAAQWFVGQIMKRTRGRANPQMALKLV
TQALEEQAQK
>Mature_489_residues
STAKWETVIGLEVHVELSTETKIWCGCKNEFGAEPNTNVCPVCLALPGALPVLNYKAVEYTIRAGLALNCKIQRHSKFDR
KNYFYADLPSGYQISQFDLPLCYDGYVDITKKDGTTRRIRIKRIHLETDAGKLLHAGDDVAAADYSLVDFNRAGVPLIEI
VTEPDLRSAEEAGLFLQKLRTILKYSGVSDVKMEEGSMRCDVNLSVRPAGSSEYGVRTELKNVNSFSAVMRGIEYEEKRH
IRILEEGGQPEQETRSWRDAQGISVLLRSKEDAEDYRYFPEPDLPPLEVSAEEIERIRAGLPELPDALMQRLMTEYGLSA
YDASVIVAEREYAQWFLHAVELAGAGQAKTVANWQINELYRVMNEKGLGPEQIPVTPEQLVGMLKLIEQGTITGKIAKTV
FDKMVETGKDAETIVKEEGLTQVADEGELLAIAREVVASNPKVFEDWKAGKQSAAQWFVGQIMKRTRGRANPQMALKLVT
QALEEQAQK

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=500, Percent_Identity=37.6, Blast_Score=319, Evalue=4e-87,
Organism=Caenorhabditis elegans, GI115534094, Length=390, Percent_Identity=34.3589743589744, Blast_Score=213, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6319391, Length=507, Percent_Identity=30.9664694280079, Blast_Score=237, Evalue=4e-63,
Organism=Drosophila melanogaster, GI45550808, Length=497, Percent_Identity=35.4124748490946, Blast_Score=286, Evalue=2e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_SYMTH (Q67KJ3)

Other databases:

- EMBL:   AP006840
- RefSeq:   YP_076649.1
- ProteinModelPortal:   Q67KJ3
- SMR:   Q67KJ3
- GeneID:   2980078
- GenomeReviews:   AP006840_GR
- KEGG:   sth:STH2820
- NMPDR:   fig|292459.1.peg.2695
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- BioCyc:   STHE292459:STH2820-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 54795; Mature: 54664

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTAKWETVIGLEVHVELSTETKIWCGCKNEFGAEPNTNVCPVCLALPGALPVLNYKAVE
CCCCCCEEEEEEEEEEEECCCCEEEECCCHHCCCCCCCCCCHHHHCCCCCCCCCCHHHEE
YTIRAGLALNCKIQRHSKFDRKNYFYADLPSGYQISQFDLPLCYDGYVDITKKDGTTRRI
EEEECCEEEEEEECCCCCCCCCCCEEEECCCCCEEEECCCCEEECCEEEEEECCCCEEEE
RIKRIHLETDAGKLLHAGDDVAAADYSLVDFNRAGVPLIEIVTEPDLRSAEEAGLFLQKL
EEEEEEEECCCCCEEECCCCCCCCCCEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHH
RTILKYSGVSDVKMEEGSMRCDVNLSVRPAGSSEYGVRTELKNVNSFSAVMRGIEYEEKR
HHHHHHCCCCCCEECCCCEEEEEEEEEECCCCCCCCCHHHHHCCHHHHHHHHCCCCCCCC
HIRILEEGGQPEQETRSWRDAQGISVLLRSKEDAEDYRYFPEPDLPPLEVSAEEIERIRA
CEEEEECCCCCHHHHHHHHHHCCCEEEEECCCCHHHCCCCCCCCCCCCCCCHHHHHHHHC
GLPELPDALMQRLMTEYGLSAYDASVIVAEREYAQWFLHAVELAGAGQAKTVANWQINEL
CCCCCHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHCCCCCCCEECCCHHHHH
YRVMNEKGLGPEQIPVTPEQLVGMLKLIEQGTITGKIAKTVFDKMVETGKDAETIVKEEG
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCC
LTQVADEGELLAIAREVVASNPKVFEDWKAGKQSAAQWFVGQIMKRTRGRANPQMALKLV
CHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
TQALEEQAQK
HHHHHHHHCC
>Mature Secondary Structure 
STAKWETVIGLEVHVELSTETKIWCGCKNEFGAEPNTNVCPVCLALPGALPVLNYKAVE
CCCCCEEEEEEEEEEEECCCCEEEECCCHHCCCCCCCCCCHHHHCCCCCCCCCCHHHEE
YTIRAGLALNCKIQRHSKFDRKNYFYADLPSGYQISQFDLPLCYDGYVDITKKDGTTRRI
EEEECCEEEEEEECCCCCCCCCCCEEEECCCCCEEEECCCCEEECCEEEEEECCCCEEEE
RIKRIHLETDAGKLLHAGDDVAAADYSLVDFNRAGVPLIEIVTEPDLRSAEEAGLFLQKL
EEEEEEEECCCCCEEECCCCCCCCCCEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHH
RTILKYSGVSDVKMEEGSMRCDVNLSVRPAGSSEYGVRTELKNVNSFSAVMRGIEYEEKR
HHHHHHCCCCCCEECCCCEEEEEEEEEECCCCCCCCCHHHHHCCHHHHHHHHCCCCCCCC
HIRILEEGGQPEQETRSWRDAQGISVLLRSKEDAEDYRYFPEPDLPPLEVSAEEIERIRA
CEEEEECCCCCHHHHHHHHHHCCCEEEEECCCCHHHCCCCCCCCCCCCCCCHHHHHHHHC
GLPELPDALMQRLMTEYGLSAYDASVIVAEREYAQWFLHAVELAGAGQAKTVANWQINEL
CCCCCHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHCCCCCCCEECCCHHHHH
YRVMNEKGLGPEQIPVTPEQLVGMLKLIEQGTITGKIAKTVFDKMVETGKDAETIVKEEG
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCC
LTQVADEGELLAIAREVVASNPKVFEDWKAGKQSAAQWFVGQIMKRTRGRANPQMALKLV
CHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
TQALEEQAQK
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA