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The map label for this gene is lacF [H]

Identifier: 50841882

GI number: 50841882

Start: 445838

End: 446794

Strand: Direct

Name: lacF [H]

Synonym: PPA0399

Alternate gene names: 50841882

Gene position: 445838-446794 (Clockwise)

Preceding gene: 50841881

Following gene: 50841883

Centisome position: 17.41

GC content: 60.61

Gene sequence:

>957_bases
ATGATTCACCCCGGCACGCGGGGCGACACCGCCGTCGCCCCACTCTCGAGCCGGTCAGGACACCGCAAGAAGAAGGACCC
GACCAGCGTCGGAGCGTGGCCGGCCCTGTTCATCGGCCCGCTCATGCTCGGCATCGCAGTGTTCTACTACTGGCCGATTT
TCGAGAACGTTGTTGCGTCGCTGAAGCAGACGAACGCTTTCGGTGGGAACCCACAGTTCGTCGGATTCGAGAACTATCGG
GAACTGTTTGCTAGTCCGGACCTCAGGTCAGCGATCGGCAACACCTTGCTCTACACGGTCATCGTCCTGCTCGGCATTCC
ACTGTCGGTGGCTGTAGCGGCCATGATCGAGCTGCCCGGCCTGAGGTTCAAGGGGCTGTACCGGATCCTCTACTTCATGC
CGTACCTTGCCATGCCGATGGCCGTCGCTCAGGTGTGGAAGATCGTCTACAACGGTCAGTTTGGTCTGCTTAACCAGATT
CTTCGGAGCGTGGGGGTCTCCGATCCGCCCTACTGGCTGGTGTCACCTGGATGGGCGTTGTTCGCTGTTGCCCTGTTTGG
GTTGTGGGCGTCGATCGGGTTCAACGTGATCATCCTGTCTGCGGGGCTGAAGTCGATCCCGCGGGAGCTGTACGAGGCCG
CGTCCATTGACGGTGCCTCTGCCTGGCGACAGTTCCGGATGGTCACGGTGCCGTTGCTGTCCCCGTCGATCTTCTTCCTG
ACGATCATGACGACGATCGGTGGGTTCCAGTTGTTCGACGCGCTGTACGCGATGATCGGAACGGGGAACCCGGCTGAGCC
GAGGACTCGCTCGTTGGTGTCGCTGTTTTACCGACAAGCCTTCGTCAACCATGACCAGGGGCTCGGTGCCGCCGTGGCGA
TCGTTATCTTTGCTCTCGTCGCCATCGTGACTCTCGCCCAGTTCCTGGGGCAGAAGAGATGGGTGAACTATGTCTGA

Upstream 100 bases:

>100_bases
CTGACTACGTCAACCCGGCATTCTCCGGTGAGATGCCTGTCCCGGAGACGGCGAGGAAGCTCGCGACGTTCATGAATGAT
GCGCTGGCGAAGGAGAAGGG

Downstream 100 bases:

>100_bases
GGTGAGTACGGGGTCCGAACTCTCCGCTGTAGAGGTGGCGGCCCTTGCCAAGCGGGGCGACACCGTCTCGCAGCGGCGCT
TCGTCGGGGTGCACATCCTT

Product: sugar ABC transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MIHPGTRGDTAVAPLSSRSGHRKKKDPTSVGAWPALFIGPLMLGIAVFYYWPIFENVVASLKQTNAFGGNPQFVGFENYR
ELFASPDLRSAIGNTLLYTVIVLLGIPLSVAVAAMIELPGLRFKGLYRILYFMPYLAMPMAVAQVWKIVYNGQFGLLNQI
LRSVGVSDPPYWLVSPGWALFAVALFGLWASIGFNVIILSAGLKSIPRELYEAASIDGASAWRQFRMVTVPLLSPSIFFL
TIMTTIGGFQLFDALYAMIGTGNPAEPRTRSLVSLFYRQAFVNHDQGLGAAVAIVIFALVAIVTLAQFLGQKRWVNYV

Sequences:

>Translated_318_residues
MIHPGTRGDTAVAPLSSRSGHRKKKDPTSVGAWPALFIGPLMLGIAVFYYWPIFENVVASLKQTNAFGGNPQFVGFENYR
ELFASPDLRSAIGNTLLYTVIVLLGIPLSVAVAAMIELPGLRFKGLYRILYFMPYLAMPMAVAQVWKIVYNGQFGLLNQI
LRSVGVSDPPYWLVSPGWALFAVALFGLWASIGFNVIILSAGLKSIPRELYEAASIDGASAWRQFRMVTVPLLSPSIFFL
TIMTTIGGFQLFDALYAMIGTGNPAEPRTRSLVSLFYRQAFVNHDQGLGAAVAIVIFALVAIVTLAQFLGQKRWVNYV
>Mature_318_residues
MIHPGTRGDTAVAPLSSRSGHRKKKDPTSVGAWPALFIGPLMLGIAVFYYWPIFENVVASLKQTNAFGGNPQFVGFENYR
ELFASPDLRSAIGNTLLYTVIVLLGIPLSVAVAAMIELPGLRFKGLYRILYFMPYLAMPMAVAQVWKIVYNGQFGLLNQI
LRSVGVSDPPYWLVSPGWALFAVALFGLWASIGFNVIILSAGLKSIPRELYEAASIDGASAWRQFRMVTVPLLSPSIFFL
TIMTTIGGFQLFDALYAMIGTGNPAEPRTRSLVSLFYRQAFVNHDQGLGAAVAIVIFALVAIVTLAQFLGQKRWVNYV

Specific function: Part of the binding-protein-dependent transport system for lactose. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1175

COG function: function code G; ABC-type sugar transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789861, Length=291, Percent_Identity=31.2714776632302, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1787570, Length=273, Percent_Identity=28.5714285714286, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1790465, Length=251, Percent_Identity=29.4820717131474, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34893; Mature: 34893

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHPGTRGDTAVAPLSSRSGHRKKKDPTSVGAWPALFIGPLMLGIAVFYYWPIFENVVAS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKQTNAFGGNPQFVGFENYRELFASPDLRSAIGNTLLYTVIVLLGIPLSVAVAAMIELPG
HHHHCCCCCCCCEECHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCC
LRFKGLYRILYFMPYLAMPMAVAQVWKIVYNGQFGLLNQILRSVGVSDPPYWLVSPGWAL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEECCCHHH
FAVALFGLWASIGFNVIILSAGLKSIPRELYEAASIDGASAWRQFRMVTVPLLSPSIFFL
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEHHHCHHHHHH
TIMTTIGGFQLFDALYAMIGTGNPAEPRTRSLVSLFYRQAFVNHDQGLGAAVAIVIFALV
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AIVTLAQFLGQKRWVNYV
HHHHHHHHHCHHHCCCCC
>Mature Secondary Structure
MIHPGTRGDTAVAPLSSRSGHRKKKDPTSVGAWPALFIGPLMLGIAVFYYWPIFENVVAS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKQTNAFGGNPQFVGFENYRELFASPDLRSAIGNTLLYTVIVLLGIPLSVAVAAMIELPG
HHHHCCCCCCCCEECHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCC
LRFKGLYRILYFMPYLAMPMAVAQVWKIVYNGQFGLLNQILRSVGVSDPPYWLVSPGWAL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEECCCHHH
FAVALFGLWASIGFNVIILSAGLKSIPRELYEAASIDGASAWRQFRMVTVPLLSPSIFFL
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEHHHCHHHHHH
TIMTTIGGFQLFDALYAMIGTGNPAEPRTRSLVSLFYRQAFVNHDQGLGAAVAIVIFALV
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AIVTLAQFLGQKRWVNYV
HHHHHHHHHCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1630315 [H]