Click here to switch to the map view.

The map label for this gene is sssIM [H]

Identifier: 50365120

GI number: 50365120

Start: 348849

End: 350216

Strand: Direct

Name: sssIM [H]

Synonym: Mfl304

Alternate gene names: 50365120

Gene position: 348849-350216 (Clockwise)

Preceding gene: 50365119

Following gene: 50365121

Centisome position: 43.98

GC content: 25.51

Gene sequence:

>1368_bases
ATGGAAAAAATAAAAGTTTTCGAAACGTTTTCAGGTATAGGTGCTCAACATAAAGCTTTAGATATTTTAAAACAAAATAA
TTATATAAATTATGAAGTTGTTGGAACGAGCGAATGAGATATATGAGCCAATATAGCGTATAATGCAATTCATAATAAAA
ATATAGATCATACAAAAAATATTAGTGAGAAAGAGATTAATGACTTTTTAATTAAATTTACACATTCAAGAGATTCAAAA
ACTCCGCTTACAAATGAACAAGTAATCAAATTACCGATAATAATAAAACAAAATTTATATAACTCTATTAAAAATTCAAA
CAATTTAGGAAGTATAGTTGGCGTTACTGGCAATATGATATCATCTCAAGTAGGTAAGATAGATTTATTAACTTATTCAT
TTCCATGCCAAGATTTATCTACTGCAGGAAGCTTTCATGGGAAAAATCAAGGAATGAAAAAAAATAGTGGAACAAGATCA
GGTCTTTTATGAGAAATTGAAAGAATATTAAAAGAACTTAAAGCAAAAAAAGAATTACCAAAATATTTGCTTTTAGAAAA
TGTTAATAACATGATTTCGGAAAGACATAAAGATGATTACTTAATGTGATTAGATTTTTTAAATTCAATTGGATATAATA
CTCAAACTTACGTATTAGAAGCAAACAAATATGGTTGCCCACAAAATCGTAAAAGAGTTTTTGCGTTGAGCGTTAGAAAA
GATGTAACTAAAGAATTTAAGGATACTTTTTTAGAAAGTAAAAGAACAAGCATTAAAGAAGAAAAAACACCATTTGATAT
TTCTAAAGAAATAGGCATATATAAAAAAACACATGAATTGCTGAGAGAGGATTATTCAATAGAAAAATACAAAAATGAAG
CAATAAATGCAATTCCTAATAGAACTGATAGTAGAAAAAGAATTTTTTTAAATAATAAAATGCTTTCAACACAGGAAAAT
ACAAATATTGAAAAACATTCTAAAAATATATATATCCAAAATGGAAAAACGTATTCAAATTTTTCTTCAACTATAACAAC
TAAACAAGATAGAGATCCTAATGGCGGAATCATAAACTTAAAAGGTACAGATTTGCAAAATAGATATAATGAAAAAAAAC
AAATTAGGAAAGCAAATTTTAGACTTTTAACGCCTAGAGAGTGTTATATGTTAATGGGTTTTTCAGAGAATGATTTTGAT
AAAGTTTTATCAAAAGCTGAGCAAATTAATTTTAGTGTAAGTGTAAGAAAAAAAACTTTTACTGACAGTATACTTTACAG
ACAAAGCGGGAACAGTATATCTGTCAATGTTTTAACTTCTATTTTTAAAGTAATTGCAGATATAGAAAATAAAACATTTA
AAGGATAA

Upstream 100 bases:

>100_bases
ATCACCCTAACCGGTGATTTTTTTATGTTTAAAAGAAGGTGCGCTGTTTTTAAAAAATAAAACTGTTATAATAATTTTAT
GCAAATAAAAAGGGGTATTC

Downstream 100 bases:

>100_bases
AAAGATATAATTTTAAAAAAGGGGTAATATGAAAAAAAGAATATTTAATAAATTTCAACCATATTTAAATGAATTTGAAA
AAGAAAAAATAGCATTAGTA

Product: deoxycytosine methylase

Products: NA

Alternate protein names: Cytosine-specific methyltransferase SssI; M.SssI [H]

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MEKIKVFETFSGIGAQHKALDILKQNNYINYEVVGTSEWDIWANIAYNAIHNKNIDHTKNISEKEINDFLIKFTHSRDSK
TPLTNEQVIKLPIIIKQNLYNSIKNSNNLGSIVGVTGNMISSQVGKIDLLTYSFPCQDLSTAGSFHGKNQGMKKNSGTRS
GLLWEIERILKELKAKKELPKYLLLENVNNMISERHKDDYLMWLDFLNSIGYNTQTYVLEANKYGCPQNRKRVFALSVRK
DVTKEFKDTFLESKRTSIKEEKTPFDISKEIGIYKKTHELLREDYSIEKYKNEAINAIPNRTDSRKRIFLNNKMLSTQEN
TNIEKHSKNIYIQNGKTYSNFSSTITTKQDRDPNGGIINLKGTDLQNRYNEKKQIRKANFRLLTPRECYMLMGFSENDFD
KVLSKAEQINFSVSVRKKTFTDSILYRQSGNSISVNVLTSIFKVIADIENKTFKG

Sequences:

>Translated_455_residues
MEKIKVFETFSGIGAQHKALDILKQNNYINYEVVGTSE*DI*ANIAYNAIHNKNIDHTKNISEKEINDFLIKFTHSRDSK
TPLTNEQVIKLPIIIKQNLYNSIKNSNNLGSIVGVTGNMISSQVGKIDLLTYSFPCQDLSTAGSFHGKNQGMKKNSGTRS
GLL*EIERILKELKAKKELPKYLLLENVNNMISERHKDDYLM*LDFLNSIGYNTQTYVLEANKYGCPQNRKRVFALSVRK
DVTKEFKDTFLESKRTSIKEEKTPFDISKEIGIYKKTHELLREDYSIEKYKNEAINAIPNRTDSRKRIFLNNKMLSTQEN
TNIEKHSKNIYIQNGKTYSNFSSTITTKQDRDPNGGIINLKGTDLQNRYNEKKQIRKANFRLLTPRECYMLMGFSENDFD
KVLSKAEQINFSVSVRKKTFTDSILYRQSGNSISVNVLTSIFKVIADIENKTFKG
>Mature_455_residues
MEKIKVFETFSGIGAQHKALDILKQNNYINYEVVGTSE*DI*ANIAYNAIHNKNIDHTKNISEKEINDFLIKFTHSRDSK
TPLTNEQVIKLPIIIKQNLYNSIKNSNNLGSIVGVTGNMISSQVGKIDLLTYSFPCQDLSTAGSFHGKNQGMKKNSGTRS
GLL*EIERILKELKAKKELPKYLLLENVNNMISERHKDDYLM*LDFLNSIGYNTQTYVLEANKYGCPQNRKRVFALSVRK
DVTKEFKDTFLESKRTSIKEEKTPFDISKEIGIYKKTHELLREDYSIEKYKNEAINAIPNRTDSRKRIFLNNKMLSTQEN
TNIEKHSKNIYIQNGKTYSNFSSTITTKQDRDPNGGIINLKGTDLQNRYNEKKQIRKANFRLLTPRECYMLMGFSENDFD
KVLSKAEQINFSVSVRKKTFTDSILYRQSGNSISVNVLTSIFKVIADIENKTFKG

Specific function: This de-novo methylase methylates completely and exclusively CG residues in Spiroplasma strain MQ-1 DNA [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 51723; Mature: 51723

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKIKVFETFSGIGAQHKALDILKQNNYINYEVVGTSEDIANIAYNAIHNKNIDHTKNIS
CCCEEEEHHHHCCCCHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCC
EKEINDFLIKFTHSRDSKTPLTNEQVIKLPIIIKQNLYNSIKNSNNLGSIVGVTGNMISS
HHHHHHHHEEEECCCCCCCCCCCCCEEEEEEEEHHHHHHHHCCCCCCCEEEECCCHHHHH
QVGKIDLLTYSFPCQDLSTAGSFHGKNQGMKKNSGTRSGLLEIERILKELKAKKELPKYL
CCCEEEEEEEECCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHH
LLENVNNMISERHKDDYLMLDFLNSIGYNTQTYVLEANKYGCPQNRKRVFALSVRKDVTK
HHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEEHHHHHHHH
EFKDTFLESKRTSIKEEKTPFDISKEIGIYKKTHELLREDYSIEKYKNEAINAIPNRTDS
HHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCC
RKRIFLNNKMLSTQENTNIEKHSKNIYIQNGKTYSNFSSTITTKQDRDPNGGIINLKGTD
CCEEEEECCEECCCCCCCHHHCCCEEEEECCCEECCCCCEECCCCCCCCCCCEEEEECCC
LQNRYNEKKQIRKANFRLLTPRECYMLMGFSENDFDKVLSKAEQINFSVSVRKKTFTDSI
HHHHHHHHHHHHHCCCEEECCHHEEEEECCCCCCHHHHHHHHHHCCEEEEEECHHHHHHH
LYRQSGNSISVNVLTSIFKVIADIENKTFKG
HEECCCCEEEHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MEKIKVFETFSGIGAQHKALDILKQNNYINYEVVGTSEDIANIAYNAIHNKNIDHTKNIS
CCCEEEEHHHHCCCCHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCC
EKEINDFLIKFTHSRDSKTPLTNEQVIKLPIIIKQNLYNSIKNSNNLGSIVGVTGNMISS
HHHHHHHHEEEECCCCCCCCCCCCCEEEEEEEEHHHHHHHHCCCCCCCEEEECCCHHHHH
QVGKIDLLTYSFPCQDLSTAGSFHGKNQGMKKNSGTRSGLLEIERILKELKAKKELPKYL
CCCEEEEEEEECCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHH
LLENVNNMISERHKDDYLMLDFLNSIGYNTQTYVLEANKYGCPQNRKRVFALSVRKDVTK
HHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEEHHHHHHHH
EFKDTFLESKRTSIKEEKTPFDISKEIGIYKKTHELLREDYSIEKYKNEAINAIPNRTDS
HHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCC
RKRIFLNNKMLSTQENTNIEKHSKNIYIQNGKTYSNFSSTITTKQDRDPNGGIINLKGTD
CCEEEEECCEECCCCCCCHHHCCCEEEEECCCEECCCCCEECCCCCCCCCCCEEEEECCC
LQNRYNEKKQIRKANFRLLTPRECYMLMGFSENDFDKVLSKAEQINFSVSVRKKTFTDSI
HHHHHHHHHHHHHCCCEEECCHHEEEEECCCCCCHHHHHHHHHHCCEEEEEECHHHHHHH
LYRQSGNSISVNVLTSIFKVIADIENKTFKG
HEECCCCEEEHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2181400 [H]