Click here to switch to the map view.

The map label for this gene is rsmE [H]

Identifier: 50365119

GI number: 50365119

Start: 347939

End: 348655

Strand: Direct

Name: rsmE [H]

Synonym: Mfl303

Alternate gene names: 50365119

Gene position: 347939-348655 (Clockwise)

Preceding gene: 50365118

Following gene: 50365120

Centisome position: 43.86

GC content: 22.73

Gene sequence:

>717_bases
ATGTTTAGATATTTTGTTGAAATTAAAAAAGATAATAATTTTTTTATTATTAATAATAACGATGTACATCATATAAAAAA
TGTTGTAAAACTTAAAAATGATGAAATTATTGAGTGTGTTTATGAAAAAGAAGTTTATCAAACTAAAATTATTGATTTAA
CTATTGAAAATAATGTAATTGTTGAAATTATTAATAAAGTAGAAACAACAATAAGCAATGTTAAAAAAGTTTTAATAGCT
GGAGTTTTAAGAGAACAAAAATGAGATTATTTATTACAAAAATCAACAGAACTTGGAGTTGATGAAATAATACCAGTTAT
TTTTAAGAGAAATGTAGTTAAAATTGATGAAAAAAAGATTGATCAAAAACTGCAAAGATGACAAGCAATTTGTGACACAG
CTGCTAAACAAGCAAAAAGGACAACAATCCCTGTTGTTAATAATTTAATAACTAATCTAAAAGATTTAAAAAACAATTTA
TGTGATTTAAATCTTGTGGCTTGAGAAAAAGAAAAAAATATTGAACTTAAAACATATTTAACACAAGACTTTAATTCAAT
TAGTTTCATTATTGGGTGTGAAGGTGGAATTGAAGTTAGTGAAATTAAAATTCTGCACGAAATAGGTTTTAAGAATGTTA
GTCTAGGAACTAATATATTAAGAGCTGAAACAGCACCAACTTATGTATTAAGTAGTTTAATTTATGAAAATAAATAA

Upstream 100 bases:

>100_bases
GATTATTATAAAAAAGATCCTAAGAATACATTATTAAAAGATTTTAAACCAGATAAAGAAAAATATGATATTTTTAAATC
AATCATTAAAGGTGAGATAA

Downstream 100 bases:

>100_bases
TCTTAGTATTTAAATTAAAAGTCAATAAGTATATAATTATAACGTGTCTGGCACCGCCTTTCCTTAATTATCAATTATAT
TTTAGCCAGACACATCACCC

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MFRYFVEIKKDNNFFIINNNDVHHIKNVVKLKNDEIIECVYEKEVYQTKIIDLTIENNVIVEIINKVETTISNVKKVLIA
GVLREQKWDYLLQKSTELGVDEIIPVIFKRNVVKIDEKKIDQKLQRWQAICDTAAKQAKRTTIPVVNNLITNLKDLKNNL
CDLNLVAWEKEKNIELKTYLTQDFNSISFIIGCEGGIEVSEIKILHEIGFKNVSLGTNILRAETAPTYVLSSLIYENK

Sequences:

>Translated_238_residues
MFRYFVEIKKDNNFFIINNNDVHHIKNVVKLKNDEIIECVYEKEVYQTKIIDLTIENNVIVEIINKVETTISNVKKVLIA
GVLREQK*DYLLQKSTELGVDEIIPVIFKRNVVKIDEKKIDQKLQR*QAICDTAAKQAKRTTIPVVNNLITNLKDLKNNL
CDLNLVA*EKEKNIELKTYLTQDFNSISFIIGCEGGIEVSEIKILHEIGFKNVSLGTNILRAETAPTYVLSSLIYENK
>Mature_238_residues
MFRYFVEIKKDNNFFIINNNDVHHIKNVVKLKNDEIIECVYEKEVYQTKIIDLTIENNVIVEIINKVETTISNVKKVLIA
GVLREQK*DYLLQKSTELGVDEIIPVIFKRNVVKIDEKKIDQKLQR*QAICDTAAKQAKRTTIPVVNNLITNLKDLKNNL
CDLNLVA*EKEKNIELKTYLTQDFNSISFIIGCEGGIEVSEIKILHEIGFKNVSLGTNILRAETAPTYVLSSLIYENK

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=244, Percent_Identity=23.7704918032787, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 26929; Mature: 26929

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRYFVEIKKDNNFFIINNNDVHHIKNVVKLKNDEIIECVYEKEVYQTKIIDLTIENNVI
CCEEEEEEEECCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEEEECCCHH
VEIINKVETTISNVKKVLIAGVLREQKDYLLQKSTELGVDEIIPVIFKRNVVKIDEKKID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEECHHHHH
QKLQRQAICDTAAKQAKRTTIPVVNNLITNLKDLKNNLCDLNLVAEKEKNIELKTYLTQD
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCC
FNSISFIIGCEGGIEVSEIKILHEIGFKNVSLGTNILRAETAPTYVLSSLIYENK
CCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCHHEECCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MFRYFVEIKKDNNFFIINNNDVHHIKNVVKLKNDEIIECVYEKEVYQTKIIDLTIENNVI
CCEEEEEEEECCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEEEECCCHH
VEIINKVETTISNVKKVLIAGVLREQKDYLLQKSTELGVDEIIPVIFKRNVVKIDEKKID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEECHHHHH
QKLQRQAICDTAAKQAKRTTIPVVNNLITNLKDLKNNLCDLNLVAEKEKNIELKTYLTQD
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCC
FNSISFIIGCEGGIEVSEIKILHEIGFKNVSLGTNILRAETAPTYVLSSLIYENK
CCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCHHEECCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9729120 [H]