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The map label for this gene is pyk [H]

Identifier: 50364991

GI number: 50364991

Start: 203834

End: 205270

Strand: Direct

Name: pyk [H]

Synonym: Mfl175

Alternate gene names: 50364991

Gene position: 203834-205270 (Clockwise)

Preceding gene: 50364990

Following gene: 50364993

Centisome position: 25.7

GC content: 32.29

Gene sequence:

>1437_bases
ATGAACAAGAAAGAATTACAAGCACGTGTAAAACGTACTAAAATTATTACTACAACTGGTCCATCAACAAATGAACCAGA
ACAAATAAGAGAATTATTTGAAAATGGAATGACAACAATACGTTTAAATTTTTCACATGGTGATTATGAAGAACAAGGTT
ACAGAATTGCTGGAGCTAAAAAAGTTAGAGAAGAATTAGGAAAACCTGTTTCTATTTTATTAGATACAAAAGGGCCTGAA
ATTCGTGTTGGTAAATTTGTAGATGGAAAACAAGAAGTTACTGCAAATCAATCAATTACAATTTACACAGATGCTGAATC
ATTCAAAAACAAAGAATGTTTATCAGGAGAAATGACTGTTGCTTATGACATGTCAGTTGATTTAAAAATTGGTGATACAA
TTTTAATCGATGATGGTAAATTAGAAATGACTGTTGAAGAAGTTAAACCAGGTGTTGTTAAAGCTATCGCTTTCAATAAC
CACTTAGTAAAAACTAACAAACGTGTTAACTTACCAGGTGTTGATTTCTCAATGCCATTCTTAGCTCAAAAAGATATCAA
TGATATTAAATATGGAGTTGAACAAGGTGTTGACTACATCGCTGCATCATTTGTTAACTCAGCTGAAAATGTAAAAGAAA
TTCGTGATATTTTAGCAGAAGCTAATGGATCAGATATTCAAATTATTTCAAAAATTGAATCACAAGTTGGAATTGACAAC
ATTGATGCAATTATTGAAGCATCAGATGGAATTATGATTGCTCGTGGTGACTTAGGATTAGAAATTCCTTACTACGACGT
ACCATACTGAGAAAAAATAATGATCAGAAAATGTCGTGAAGCTGGTAAAGTTGTTATTGTTGCTACTCAAATGTTAGAAA
CAATGACAGAAAACCCAGCACCTACAAGAGCTGAAGTAACTGACGTTTACTTTGCTACTGAATTAGGAGCAGATGCAACA
ATGTTATCTGGAGAATCAGCAGCTGGAGATTACCCATTCATTACTGTTAACACAATGGCAACAATCAACAAACGTGCTGA
AATAGAATTCTACAAAAAAGCATACTACCAAACTCAATTAGAAAACGCTAAAAACTCAACTAGTGGACCACGTGCAGATA
TTGCAATGGACTTAGCTGAAAGAACTCGTGATGGACAATATGAATTTGCTGTTGTTTTATCAAGAACTGGAGCTTTATTA
AAAACTATTTCTAAATTTAGACCAAATGTAACAATTTTAGGTGTAAGTGAATCAGAAAGATTATGAACTGCATTTGGTGT
ATGACACTCAATCTTCATGAACAAAACTGCTAACTTATTAGAATTAGAAGAAAATGTTGCTCAAATCTCAGAAATTGCAA
AATTATGAGGTGCAAAATCAGGATCAAAAATCTTAGTTGTAAGAAACAAAGATATTCGTGAAATTACAGTAGCTTAA

Upstream 100 bases:

>100_bases
CAGAATTTATCGAAAAAATAAGATTATTGAACGCAAAGATATCAAAATAGGAAAAAATAATTTAAAATATATATTGAAAA
AGTACATAGGAGAAAATAAA

Downstream 100 bases:

>100_bases
TTAAAATAATAAAAAACTACCGCAAGGTAGATTTTTTTTTATTTATTAAGAGATTTTCTCAATAGCAGCTTTAGATACTT
TATTTTTAAATCACTTTTGA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MNKKELQARVKRTKIITTTGPSTNEPEQIRELFENGMTTIRLNFSHGDYEEQGYRIAGAKKVREELGKPVSILLDTKGPE
IRVGKFVDGKQEVTANQSITIYTDAESFKNKECLSGEMTVAYDMSVDLKIGDTILIDDGKLEMTVEEVKPGVVKAIAFNN
HLVKTNKRVNLPGVDFSMPFLAQKDINDIKYGVEQGVDYIAASFVNSAENVKEIRDILAEANGSDIQIISKIESQVGIDN
IDAIIEASDGIMIARGDLGLEIPYYDVPYWEKIMIRKCREAGKVVIVATQMLETMTENPAPTRAEVTDVYFATELGADAT
MLSGESAAGDYPFITVNTMATINKRAEIEFYKKAYYQTQLENAKNSTSGPRADIAMDLAERTRDGQYEFAVVLSRTGALL
KTISKFRPNVTILGVSESERLWTAFGVWHSIFMNKTANLLELEENVAQISEIAKLWGAKSGSKILVVRNKDIREITVA

Sequences:

>Translated_478_residues
MNKKELQARVKRTKIITTTGPSTNEPEQIRELFENGMTTIRLNFSHGDYEEQGYRIAGAKKVREELGKPVSILLDTKGPE
IRVGKFVDGKQEVTANQSITIYTDAESFKNKECLSGEMTVAYDMSVDLKIGDTILIDDGKLEMTVEEVKPGVVKAIAFNN
HLVKTNKRVNLPGVDFSMPFLAQKDINDIKYGVEQGVDYIAASFVNSAENVKEIRDILAEANGSDIQIISKIESQVGIDN
IDAIIEASDGIMIARGDLGLEIPYYDVPY*EKIMIRKCREAGKVVIVATQMLETMTENPAPTRAEVTDVYFATELGADAT
MLSGESAAGDYPFITVNTMATINKRAEIEFYKKAYYQTQLENAKNSTSGPRADIAMDLAERTRDGQYEFAVVLSRTGALL
KTISKFRPNVTILGVSESERL*TAFGV*HSIFMNKTANLLELEENVAQISEIAKL*GAKSGSKILVVRNKDIREITVA
>Mature_478_residues
MNKKELQARVKRTKIITTTGPSTNEPEQIRELFENGMTTIRLNFSHGDYEEQGYRIAGAKKVREELGKPVSILLDTKGPE
IRVGKFVDGKQEVTANQSITIYTDAESFKNKECLSGEMTVAYDMSVDLKIGDTILIDDGKLEMTVEEVKPGVVKAIAFNN
HLVKTNKRVNLPGVDFSMPFLAQKDINDIKYGVEQGVDYIAASFVNSAENVKEIRDILAEANGSDIQIISKIESQVGIDN
IDAIIEASDGIMIARGDLGLEIPYYDVPY*EKIMIRKCREAGKVVIVATQMLETMTENPAPTRAEVTDVYFATELGADAT
MLSGESAAGDYPFITVNTMATINKRAEIEFYKKAYYQTQLENAKNSTSGPRADIAMDLAERTRDGQYEFAVVLSRTGALL
KTISKFRPNVTILGVSESERL*TAFGV*HSIFMNKTANLLELEENVAQISEIAKL*GAKSGSKILVVRNKDIREITVA

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=425, Percent_Identity=37.4117647058824, Blast_Score=266, Evalue=4e-71,
Organism=Homo sapiens, GI33286420, Length=425, Percent_Identity=37.4117647058824, Blast_Score=266, Evalue=4e-71,
Organism=Homo sapiens, GI33286418, Length=425, Percent_Identity=37.6470588235294, Blast_Score=265, Evalue=7e-71,
Organism=Homo sapiens, GI32967597, Length=419, Percent_Identity=37.4701670644391, Blast_Score=262, Evalue=5e-70,
Organism=Homo sapiens, GI10835121, Length=419, Percent_Identity=37.4701670644391, Blast_Score=262, Evalue=6e-70,
Organism=Homo sapiens, GI310128732, Length=288, Percent_Identity=38.8888888888889, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI310128730, Length=288, Percent_Identity=38.8888888888889, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI310128736, Length=236, Percent_Identity=34.7457627118644, Blast_Score=139, Evalue=8e-33,
Organism=Homo sapiens, GI310128734, Length=236, Percent_Identity=34.7457627118644, Blast_Score=139, Evalue=8e-33,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=34.4186046511628, Blast_Score=121, Evalue=2e-27,
Organism=Escherichia coli, GI1787965, Length=426, Percent_Identity=42.7230046948357, Blast_Score=317, Evalue=8e-88,
Organism=Escherichia coli, GI1788160, Length=346, Percent_Identity=39.3063583815029, Blast_Score=208, Evalue=7e-55,
Organism=Caenorhabditis elegans, GI17544584, Length=419, Percent_Identity=39.1408114558473, Blast_Score=283, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI71984413, Length=422, Percent_Identity=38.1516587677725, Blast_Score=257, Evalue=8e-69,
Organism=Caenorhabditis elegans, GI17506829, Length=422, Percent_Identity=38.1516587677725, Blast_Score=257, Evalue=9e-69,
Organism=Caenorhabditis elegans, GI17506831, Length=428, Percent_Identity=38.0841121495327, Blast_Score=256, Evalue=1e-68,
Organism=Caenorhabditis elegans, GI71984406, Length=422, Percent_Identity=38.1516587677725, Blast_Score=256, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6319279, Length=423, Percent_Identity=41.1347517730496, Blast_Score=275, Evalue=1e-74,
Organism=Saccharomyces cerevisiae, GI6324923, Length=422, Percent_Identity=41.2322274881517, Blast_Score=272, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24648964, Length=419, Percent_Identity=39.618138424821, Blast_Score=259, Evalue=3e-69,
Organism=Drosophila melanogaster, GI28571814, Length=419, Percent_Identity=39.618138424821, Blast_Score=259, Evalue=3e-69,
Organism=Drosophila melanogaster, GI24648966, Length=409, Percent_Identity=38.8753056234719, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24581235, Length=424, Percent_Identity=31.8396226415094, Blast_Score=203, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24646914, Length=250, Percent_Identity=38.8, Blast_Score=152, Evalue=4e-37,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 52269; Mature: 52269

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKELQARVKRTKIITTTGPSTNEPEQIRELFENGMTTIRLNFSHGDYEEQGYRIAGAK
CCHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHCCCEEEEEEECCCCHHHCCEEEECHH
KVREELGKPVSILLDTKGPEIRVGKFVDGKQEVTANQSITIYTDAESFKNKECLSGEMTV
HHHHHHCCCEEEEEECCCCCEEEECCCCCCHHCCCCCEEEEEECHHHHCCCHHCCCCEEE
AYDMSVDLKIGDTILIDDGKLEMTVEEVKPGVVKAIAFNNHLVKTNKRVNLPGVDFSMPF
EEEEEEEEEECCEEEEECCCEEEEHHHCCCCEEEEEEECCEEEECCCEECCCCCCCCCCC
LAQKDINDIKYGVEQGVDYIAASFVNSAENVKEIRDILAEANGSDIQIISKIESQVGIDN
HHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCC
IDAIIEASDGIMIARGDLGLEIPYYDVPYEKIMIRKCREAGKVVIVATQMLETMTENPAP
CCEEEECCCCEEEEECCCCEECCCCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHCCCCC
TRAEVTDVYFATELGADATMLSGESAAGDYPFITVNTMATINKRAEIEFYKKAYYQTQLE
CCCEEEEEEEEECCCCCEEEECCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHH
NAKNSTSGPRADIAMDLAERTRDGQYEFAVVLSRTGALLKTISKFRPNVTILGVSESERL
CCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHCCCEEEEECCCCCCE
TAFGVHSIFMNKTANLLELEENVAQISEIAKLGAKSGSKILVVRNKDIREITVA
EEEEHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECC
>Mature Secondary Structure
MNKKELQARVKRTKIITTTGPSTNEPEQIRELFENGMTTIRLNFSHGDYEEQGYRIAGAK
CCHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHCCCEEEEEEECCCCHHHCCEEEECHH
KVREELGKPVSILLDTKGPEIRVGKFVDGKQEVTANQSITIYTDAESFKNKECLSGEMTV
HHHHHHCCCEEEEEECCCCCEEEECCCCCCHHCCCCCEEEEEECHHHHCCCHHCCCCEEE
AYDMSVDLKIGDTILIDDGKLEMTVEEVKPGVVKAIAFNNHLVKTNKRVNLPGVDFSMPF
EEEEEEEEEECCEEEEECCCEEEEHHHCCCCEEEEEEECCEEEECCCEECCCCCCCCCCC
LAQKDINDIKYGVEQGVDYIAASFVNSAENVKEIRDILAEANGSDIQIISKIESQVGIDN
HHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCC
IDAIIEASDGIMIARGDLGLEIPYYDVPYEKIMIRKCREAGKVVIVATQMLETMTENPAP
CCEEEECCCCEEEEECCCCEECCCCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHCCCCC
TRAEVTDVYFATELGADATMLSGESAAGDYPFITVNTMATINKRAEIEFYKKAYYQTQLE
CCCEEEEEEEEECCCCCEEEECCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHH
NAKNSTSGPRADIAMDLAERTRDGQYEFAVVLSRTGALLKTISKFRPNVTILGVSESERL
CCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHCCCEEEEECCCCCCE
TAFGVHSIFMNKTANLLELEENVAQISEIAKLGAKSGSKILVVRNKDIREITVA
EEEEHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]