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The map label for this gene is pfkA [H]

Identifier: 50364990

GI number: 50364990

Start: 202812

End: 203783

Strand: Direct

Name: pfkA [H]

Synonym: Mfl174

Alternate gene names: 50364990

Gene position: 202812-203783 (Clockwise)

Preceding gene: 50364989

Following gene: 50364991

Centisome position: 25.57

GC content: 33.02

Gene sequence:

>972_bases
ATGAAAAGAATTGGAGTTTTAACTTCAGGAGGAGATGCACCTGGAATGAATAATGCCATTGCTGGAGTTGTTAAAGCAGC
TGAAGCACAAGGTATTGAAGTTTATGGTGTAAGAGATGGGTATAAAGGTTTAATTAATGGTTGATTTGAAAAATTAGATT
CTAAATTTGCTTTAGAAATTATTTCAAAAGGTGGGACTGTTTTAGGTTCAGCTAGATTACCAGAATTCAAAGAAGAAAGT
GTAAGACAAAAAGCTGTTGATCAATTAAAAGCACATGACATTGAAGCATTAGTTGTTATTGGTGGAGATGGAAGCTACAT
GGGAGCTCAAAGATTAACTGAAATGGGAATAAACTGTATTGGTTTACCAGGAACAATAGATAATGATATTGTTTCAAGTG
ATTATACAATTGGATTTGATACAGCATTAAATACAGTTATTGAATCAGTTGAAAAAGTGCGTGATACTATGCAATCACAC
AATCGTGCTGCCGTAATTGAAGTTATGGGAAATGGTTGTGGAGATTTAGTTACTTATGCAGCTATTGCAACTCAAGCAGA
AATATTTTCACCAAGTGAAAGTAAATTAACTGTTGAACAAATATGTGAACAAGCTAAAAAATTTGCAGAAGCAAACCACA
GAAGTTTAATAATATTAATTAGTGAAAAACAACTTGATGCTAATGAATTAGCAAAAAAAGTTGAAGAAGCAAGTGGTTAT
GTAACTAGAGCAACAATTTTAGGACATGTTCAAAGAGGTGGGCGCCCATCAGGTATGGATCGCTATTTAGCTTTTACAGC
AGCAATTTATGCAGTTGAAAAATTAATTGAAGGTAAAGGTGGACTTTACATTGGTCTAAGTGAAAACAAACTTGTTGCAA
GAGATATTGAGTCAACATTGAATATGCCAAAGCCAGATAAGACAGAATTTATCGAAAAAATAAGATTATTGAACGCAAAG
ATATCAAAATAG

Upstream 100 bases:

>100_bases
TTATTATTTATTTCCACATTTATTGCAAAAAGATAAAGCAATAAAAAAGGATGTTTAGTATAATTAGTAGGTATTCTTAT
TTTATGAAAGAAGGGGTAAT

Downstream 100 bases:

>100_bases
GAAAAAATAATTTAAAATATATATTGAAAAAGTACATAGGAGAAAATAAAATGAACAAGAAAGAATTACAAGCACGTGTA
AAACGTACTAAAATTATTAC

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MKRIGVLTSGGDAPGMNNAIAGVVKAAEAQGIEVYGVRDGYKGLINGWFEKLDSKFALEIISKGGTVLGSARLPEFKEES
VRQKAVDQLKAHDIEALVVIGGDGSYMGAQRLTEMGINCIGLPGTIDNDIVSSDYTIGFDTALNTVIESVEKVRDTMQSH
NRAAVIEVMGNGCGDLVTYAAIATQAEIFSPSESKLTVEQICEQAKKFAEANHRSLIILISEKQLDANELAKKVEEASGY
VTRATILGHVQRGGRPSGMDRYLAFTAAIYAVEKLIEGKGGLYIGLSENKLVARDIESTLNMPKPDKTEFIEKIRLLNAK
ISK

Sequences:

>Translated_323_residues
MKRIGVLTSGGDAPGMNNAIAGVVKAAEAQGIEVYGVRDGYKGLING*FEKLDSKFALEIISKGGTVLGSARLPEFKEES
VRQKAVDQLKAHDIEALVVIGGDGSYMGAQRLTEMGINCIGLPGTIDNDIVSSDYTIGFDTALNTVIESVEKVRDTMQSH
NRAAVIEVMGNGCGDLVTYAAIATQAEIFSPSESKLTVEQICEQAKKFAEANHRSLIILISEKQLDANELAKKVEEASGY
VTRATILGHVQRGGRPSGMDRYLAFTAAIYAVEKLIEGKGGLYIGLSENKLVARDIESTLNMPKPDKTEFIEKIRLLNAK
ISK
>Mature_323_residues
MKRIGVLTSGGDAPGMNNAIAGVVKAAEAQGIEVYGVRDGYKGLING*FEKLDSKFALEIISKGGTVLGSARLPEFKEES
VRQKAVDQLKAHDIEALVVIGGDGSYMGAQRLTEMGINCIGLPGTIDNDIVSSDYTIGFDTALNTVIESVEKVRDTMQSH
NRAAVIEVMGNGCGDLVTYAAIATQAEIFSPSESKLTVEQICEQAKKFAEANHRSLIILISEKQLDANELAKKVEEASGY
VTRATILGHVQRGGRPSGMDRYLAFTAAIYAVEKLIEGKGGLYIGLSENKLVARDIESTLNMPKPDKTEFIEKIRLLNAK
ISK

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=359, Percent_Identity=36.2116991643454, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI266453768, Length=359, Percent_Identity=35.0974930362117, Blast_Score=176, Evalue=4e-44,
Organism=Homo sapiens, GI266453748, Length=359, Percent_Identity=35.0974930362117, Blast_Score=176, Evalue=4e-44,
Organism=Homo sapiens, GI4505749, Length=359, Percent_Identity=35.0974930362117, Blast_Score=176, Evalue=4e-44,
Organism=Homo sapiens, GI266453619, Length=359, Percent_Identity=35.0974930362117, Blast_Score=176, Evalue=4e-44,
Organism=Homo sapiens, GI48762920, Length=342, Percent_Identity=35.9649122807018, Blast_Score=173, Evalue=2e-43,
Organism=Escherichia coli, GI1790350, Length=313, Percent_Identity=50.1597444089457, Blast_Score=305, Evalue=3e-84,
Organism=Caenorhabditis elegans, GI17558788, Length=310, Percent_Identity=36.1290322580645, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI25147584, Length=329, Percent_Identity=36.1702127659575, Blast_Score=177, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI72003601, Length=310, Percent_Identity=29.0322580645161, Blast_Score=120, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6321679, Length=346, Percent_Identity=35.5491329479769, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6323861, Length=345, Percent_Identity=33.3333333333333, Blast_Score=168, Evalue=8e-43,
Organism=Drosophila melanogaster, GI28573326, Length=311, Percent_Identity=36.3344051446945, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI24652337, Length=311, Percent_Identity=35.048231511254, Blast_Score=178, Evalue=5e-45,
Organism=Drosophila melanogaster, GI17647809, Length=311, Percent_Identity=35.048231511254, Blast_Score=177, Evalue=6e-45,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 34609; Mature: 34609

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIGVLTSGGDAPGMNNAIAGVVKAAEAQGIEVYGVRDGYKGLINGFEKLDSKFALEII
CCCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHH
SKGGTVLGSARLPEFKEESVRQKAVDQLKAHDIEALVVIGGDGSYMGAQRLTEMGINCIG
HCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHCCCEEEE
LPGTIDNDIVSSDYTIGFDTALNTVIESVEKVRDTMQSHNRAAVIEVMGNGCGDLVTYAA
CCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHH
IATQAEIFSPSESKLTVEQICEQAKKFAEANHRSLIILISEKQLDANELAKKVEEASGYV
HHHHHHHCCCCCCCEEHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCE
TRATILGHVQRGGRPSGMDRYLAFTAAIYAVEKLIEGKGGLYIGLSENKLVARDIESTLN
EHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCC
MPKPDKTEFIEKIRLLNAKISK
CCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKRIGVLTSGGDAPGMNNAIAGVVKAAEAQGIEVYGVRDGYKGLINGFEKLDSKFALEII
CCCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHH
SKGGTVLGSARLPEFKEESVRQKAVDQLKAHDIEALVVIGGDGSYMGAQRLTEMGINCIG
HCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHCCCEEEE
LPGTIDNDIVSSDYTIGFDTALNTVIESVEKVRDTMQSHNRAAVIEVMGNGCGDLVTYAA
CCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHH
IATQAEIFSPSESKLTVEQICEQAKKFAEANHRSLIILISEKQLDANELAKKVEEASGYV
HHHHHHHCCCCCCCEEHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCE
TRATILGHVQRGGRPSGMDRYLAFTAAIYAVEKLIEGKGGLYIGLSENKLVARDIESTLN
EHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCC
MPKPDKTEFIEKIRLLNAKISK
CCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA