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The map label for this gene is thrS [H]

Identifier: 50364993

GI number: 50364993

Start: 206087

End: 208021

Strand: Direct

Name: thrS [H]

Synonym: Mfl177

Alternate gene names: 50364993

Gene position: 206087-208021 (Clockwise)

Preceding gene: 50364991

Following gene: 50364994

Centisome position: 25.98

GC content: 27.75

Gene sequence:

>1935_bases
ATGAAAATTAAATTATTAGATGGATCAATTAAGGAATTTGATCAAGCAACAAACGTTAGAACAATAGCTGAAACAATAGC
TATAAGTTTAAAAAAAGCTACTGCGGCAGCAAAAGTAAATGGAAGATATGTGTCAGTAGATTATGTTATTGAAAAAGATT
CAAATTTAGAATTGATAACAAATAGACATGAAGACTTTTACAAAACATTGAATTATACTGCAGCTTTTGTTACAGGTGTT
GCTATTTCAGAATTATATAAAGATGTTAAGCTTGCAAAAGTTTTATATAAAGAAGATGAACTTGAATATGGAATTACTTT
TGAAGTTGAACCAAGAATTGGTTTAGAAGAATTACAAAACATTCAAAATAAAGTTAATCAAATAATTAAAAATGAATCAA
TTTTATCTAAGGAAATGGATTTAAAAGAAGCTGAAGAAATTTTTAAAGGAAATCAGTATCAATTACATTTAGCAAAGGAA
ATGTTTGAAACCTATGGTGAAATAACTGTTTATACATTAAATGGTGTTTCAATAGTTTCTAAACACCCAATAACTTTAAA
TTTAAAAAATATAAAAGTTGTTGAGGTTCAACAATTAACTGGTGAATATTGATTAAATGATTCTAAAAATATCATGTTAC
AAAAAGTTCATGGTATGGCCGCAGAAAGTATTGAAACTTTGTCTTCTAAAAAAGAAATTCTAGAAGATAGAAGAAGTAGA
GACCACAGAATTATAAACAAAACTTTAAAAATATTTGGTTTTGATCATTTAGTAGGTCCTGGATTGCCTTTATGAATGCC
AAATGGAACAATCGTTAAAGAAGAAATCAAAAAGTATCTTAAAGAAAAAGAATGAGAATATGACTACATTAATGTAACTA
CTCCAGTTATAGGGACTGTTGAATTGTATAAAAAATCAGGACACTGAGACCACTATGGAGAAGATATGTTCCAACCATTT
AATGGTGGAAGTGGTAGTGATGAACAATTCATTTTAAGACCAATGAACTGTCCACACCACGTTGCTGTTTATAAACAAGA
ACAAAGAAGTTACAGAGATTTACCTTTAAGAATTGCTGAACATGCTTTACAACATAGATATGAATCAAGTGGTAGTTTAA
CAGGATTAGAACGTGTTAGAGCGATGGAATTAACTGATAGCCATATTTTTGTTAGACCAGACCAAGTTGAAGCAGAATTT
AAATCAATTTATAAATTAATTACTGAAGTATTAGAAACATTTAATATTCAAATTGATTACTTAAGCTTAAGTTTAAGAGA
TCCAGAAGATAAAGTTAAATACTATCAAGATGATAAAATGTGAGACGAAGCTGAGGCAGCTTTAGAAAAAGTTTTACAAG
ACTTAAATATTGATTATAAGAAATGTATTGGTGAAGCAGCATTCTATGGTCCTAAATTAGACATACAAATTAAAACAGCT
CAAAATCATGAAATAACTGTTTCTACAATTCAATTAGACTTCTTACTACCAAAGAAATTTGATGTAACATATATTGACCA
AAATCAAGAATTTAAAGCTCCAATCATGATTCATAGAGGTTTAATTGGAACTTATGAAAGATTTATTGCAACATTGTTAG
AACAAACTAAAGGTGTATTACCATTATGATTGGCTCCTAATCAAATTGAAATTATTCCTGTTGGAAGTGAAGAAAATATT
GAGTATGCAAATGAAGTAAAAAAACAATTAAAAAAAGATTTTATTAGAACTCATGTAGATTTAAGAGATGAAAGATTAAG
TTACAAAATACGTGATGCTCAAACAAGTAAAATACCTTATCAATTAGTTCTTGGTGATCAAGAAAGAAAAGATAATTCTG
TTACTTACCGTCAATATGGTAGCGAGCAACAAACAACAATTAAGTTAAATGAATTTAAAAATATGATTACAGATTTAATT
TGTAATAAGAAGTAG

Upstream 100 bases:

>100_bases
TAAATTAAGGTATTAAGTTTTTTAATACGAATTAGGATGGTACCGCGTTATAAACGCTCCTAAATATTAGGGGCGTTTTT
GTTTTTTTAAGGAGGAAAAT

Downstream 100 bases:

>100_bases
TTTGATATAAACTATAAATACAAAGGAGAAAAAGATTATGGCAGAAATGATTAAAGTTTCAACAAAAGAAGAATTTGACA
AAATTATTAGCGAAGGTAAA

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 644; Mature: 644

Protein sequence:

>644_residues
MKIKLLDGSIKEFDQATNVRTIAETIAISLKKATAAAKVNGRYVSVDYVIEKDSNLELITNRHEDFYKTLNYTAAFVTGV
AISELYKDVKLAKVLYKEDELEYGITFEVEPRIGLEELQNIQNKVNQIIKNESILSKEMDLKEAEEIFKGNQYQLHLAKE
MFETYGEITVYTLNGVSIVSKHPITLNLKNIKVVEVQQLTGEYWLNDSKNIMLQKVHGMAAESIETLSSKKEILEDRRSR
DHRIINKTLKIFGFDHLVGPGLPLWMPNGTIVKEEIKKYLKEKEWEYDYINVTTPVIGTVELYKKSGHWDHYGEDMFQPF
NGGSGSDEQFILRPMNCPHHVAVYKQEQRSYRDLPLRIAEHALQHRYESSGSLTGLERVRAMELTDSHIFVRPDQVEAEF
KSIYKLITEVLETFNIQIDYLSLSLRDPEDKVKYYQDDKMWDEAEAALEKVLQDLNIDYKKCIGEAAFYGPKLDIQIKTA
QNHEITVSTIQLDFLLPKKFDVTYIDQNQEFKAPIMIHRGLIGTYERFIATLLEQTKGVLPLWLAPNQIEIIPVGSEENI
EYANEVKKQLKKDFIRTHVDLRDERLSYKIRDAQTSKIPYQLVLGDQERKDNSVTYRQYGSEQQTTIKLNEFKNMITDLI
CNKK

Sequences:

>Translated_644_residues
MKIKLLDGSIKEFDQATNVRTIAETIAISLKKATAAAKVNGRYVSVDYVIEKDSNLELITNRHEDFYKTLNYTAAFVTGV
AISELYKDVKLAKVLYKEDELEYGITFEVEPRIGLEELQNIQNKVNQIIKNESILSKEMDLKEAEEIFKGNQYQLHLAKE
MFETYGEITVYTLNGVSIVSKHPITLNLKNIKVVEVQQLTGEY*LNDSKNIMLQKVHGMAAESIETLSSKKEILEDRRSR
DHRIINKTLKIFGFDHLVGPGLPL*MPNGTIVKEEIKKYLKEKE*EYDYINVTTPVIGTVELYKKSGH*DHYGEDMFQPF
NGGSGSDEQFILRPMNCPHHVAVYKQEQRSYRDLPLRIAEHALQHRYESSGSLTGLERVRAMELTDSHIFVRPDQVEAEF
KSIYKLITEVLETFNIQIDYLSLSLRDPEDKVKYYQDDKM*DEAEAALEKVLQDLNIDYKKCIGEAAFYGPKLDIQIKTA
QNHEITVSTIQLDFLLPKKFDVTYIDQNQEFKAPIMIHRGLIGTYERFIATLLEQTKGVLPL*LAPNQIEIIPVGSEENI
EYANEVKKQLKKDFIRTHVDLRDERLSYKIRDAQTSKIPYQLVLGDQERKDNSVTYRQYGSEQQTTIKLNEFKNMITDLI
CNKK
>Mature_644_residues
MKIKLLDGSIKEFDQATNVRTIAETIAISLKKATAAAKVNGRYVSVDYVIEKDSNLELITNRHEDFYKTLNYTAAFVTGV
AISELYKDVKLAKVLYKEDELEYGITFEVEPRIGLEELQNIQNKVNQIIKNESILSKEMDLKEAEEIFKGNQYQLHLAKE
MFETYGEITVYTLNGVSIVSKHPITLNLKNIKVVEVQQLTGEY*LNDSKNIMLQKVHGMAAESIETLSSKKEILEDRRSR
DHRIINKTLKIFGFDHLVGPGLPL*MPNGTIVKEEIKKYLKEKE*EYDYINVTTPVIGTVELYKKSGH*DHYGEDMFQPF
NGGSGSDEQFILRPMNCPHHVAVYKQEQRSYRDLPLRIAEHALQHRYESSGSLTGLERVRAMELTDSHIFVRPDQVEAEF
KSIYKLITEVLETFNIQIDYLSLSLRDPEDKVKYYQDDKM*DEAEAALEKVLQDLNIDYKKCIGEAAFYGPKLDIQIKTA
QNHEITVSTIQLDFLLPKKFDVTYIDQNQEFKAPIMIHRGLIGTYERFIATLLEQTKGVLPL*LAPNQIEIIPVGSEENI
EYANEVKKQLKKDFIRTHVDLRDERLSYKIRDAQTSKIPYQLVLGDQERKDNSVTYRQYGSEQQTTIKLNEFKNMITDLI
CNKK

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38570111, Length=601, Percent_Identity=32.1131447587354, Blast_Score=284, Evalue=2e-76,
Organism=Homo sapiens, GI38202255, Length=623, Percent_Identity=30.1765650080257, Blast_Score=268, Evalue=1e-71,
Organism=Homo sapiens, GI20070344, Length=641, Percent_Identity=30.1092043681747, Blast_Score=264, Evalue=2e-70,
Organism=Escherichia coli, GI1788013, Length=633, Percent_Identity=31.911532385466, Blast_Score=334, Evalue=1e-92,
Organism=Caenorhabditis elegans, GI71984184, Length=623, Percent_Identity=30.8186195826645, Blast_Score=257, Evalue=1e-68,
Organism=Caenorhabditis elegans, GI71984192, Length=622, Percent_Identity=30.7073954983923, Blast_Score=257, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6322112, Length=642, Percent_Identity=30.5295950155763, Blast_Score=265, Evalue=1e-71,
Organism=Saccharomyces cerevisiae, GI6322654, Length=390, Percent_Identity=32.0512820512821, Blast_Score=206, Evalue=1e-53,
Organism=Drosophila melanogaster, GI24583842, Length=622, Percent_Identity=31.3504823151125, Blast_Score=288, Evalue=1e-77,
Organism=Drosophila melanogaster, GI28574141, Length=622, Percent_Identity=31.3504823151125, Blast_Score=288, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24583839, Length=622, Percent_Identity=31.3504823151125, Blast_Score=288, Evalue=1e-77,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 73445; Mature: 73445

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKLLDGSIKEFDQATNVRTIAETIAISLKKATAAAKVNGRYVSVDYVIEKDSNLELIT
CEEEEECCCHHHHHHHCCHHHHHHHHHHHHHHHHHEEEECCEEEEEEEEEECCCCEEEEE
NRHEDFYKTLNYTAAFVTGVAISELYKDVKLAKVLYKEDELEYGITFEVEPRIGLEELQN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHH
IQNKVNQIIKNESILSKEMDLKEAEEIFKGNQYQLHLAKEMFETYGEITVYTLNGVSIVS
HHHHHHHHHHCHHHHHHHCCHHHHHHHHCCCCEEEHHHHHHHHHHCCEEEEEECCEEEEE
KHPITLNLKNIKVVEVQQLTGEYLNDSKNIMLQKVHGMAAESIETLSSKKEILEDRRSRD
CCCEEEEECCEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
HRIINKTLKIFGFDHLVGPGLPLMPNGTIVKEEIKKYLKEKEEYDYINVTTPVIGTVELY
HHHHHHHHHHHCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCEEHHHHH
KKSGHDHYGEDMFQPFNGGSGSDEQFILRPMNCPHHVAVYKQEQRSYRDLPLRIAEHALQ
HCCCCCCCCHHHCCCCCCCCCCCCCEEEECCCCCCHHHEEEHHHHHHCCCCHHHHHHHHH
HRYESSGSLTGLERVRAMELTDSHIFVRPDQVEAEFKSIYKLITEVLETFNIQIDYLSLS
HHHCCCCCCHHHHHHHHEEECCCEEEECCHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEE
LRDPEDKVKYYQDDKMDEAEAALEKVLQDLNIDYKKCIGEAAFYGPKLDIQIKTAQNHEI
ECCCHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEEEECCCCEE
TVSTIQLDFLLPKKFDVTYIDQNQEFKAPIMIHRGLIGTYERFIATLLEQTKGVLPLLAP
EEEEEEEEEECCCCCCEEEECCCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCEEEECC
NQIEIIPVGSEENIEYANEVKKQLKKDFIRTHVDLRDERLSYKIRDAQTSKIPYQLVLGD
CEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHEEEEECCCCCCCCEEEEECC
QERKDNSVTYRQYGSEQQTTIKLNEFKNMITDLICNKK
CCCCCCCCCHHHCCCCCCEEEEHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKIKLLDGSIKEFDQATNVRTIAETIAISLKKATAAAKVNGRYVSVDYVIEKDSNLELIT
CEEEEECCCHHHHHHHCCHHHHHHHHHHHHHHHHHEEEECCEEEEEEEEEECCCCEEEEE
NRHEDFYKTLNYTAAFVTGVAISELYKDVKLAKVLYKEDELEYGITFEVEPRIGLEELQN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHH
IQNKVNQIIKNESILSKEMDLKEAEEIFKGNQYQLHLAKEMFETYGEITVYTLNGVSIVS
HHHHHHHHHHCHHHHHHHCCHHHHHHHHCCCCEEEHHHHHHHHHHCCEEEEEECCEEEEE
KHPITLNLKNIKVVEVQQLTGEYLNDSKNIMLQKVHGMAAESIETLSSKKEILEDRRSRD
CCCEEEEECCEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
HRIINKTLKIFGFDHLVGPGLPLMPNGTIVKEEIKKYLKEKEEYDYINVTTPVIGTVELY
HHHHHHHHHHHCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCEEHHHHH
KKSGHDHYGEDMFQPFNGGSGSDEQFILRPMNCPHHVAVYKQEQRSYRDLPLRIAEHALQ
HCCCCCCCCHHHCCCCCCCCCCCCCEEEECCCCCCHHHEEEHHHHHHCCCCHHHHHHHHH
HRYESSGSLTGLERVRAMELTDSHIFVRPDQVEAEFKSIYKLITEVLETFNIQIDYLSLS
HHHCCCCCCHHHHHHHHEEECCCEEEECCHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEE
LRDPEDKVKYYQDDKMDEAEAALEKVLQDLNIDYKKCIGEAAFYGPKLDIQIKTAQNHEI
ECCCHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEEEECCCCEE
TVSTIQLDFLLPKKFDVTYIDQNQEFKAPIMIHRGLIGTYERFIATLLEQTKGVLPLLAP
EEEEEEEEEECCCCCCEEEECCCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCEEEECC
NQIEIIPVGSEENIEYANEVKKQLKKDFIRTHVDLRDERLSYKIRDAQTSKIPYQLVLGD
CEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHEEEEECCCCCCCCEEEEECC
QERKDNSVTYRQYGSEQQTTIKLNEFKNMITDLICNKK
CCCCCCCCCHHHCCCCCCEEEEHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA