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The map label for this gene is pfkA

Identifier: 49187488

GI number: 49187488

Start: 4408689

End: 4409648

Strand: Reverse

Name: pfkA

Synonym: BAS4493

Alternate gene names: 49187488

Gene position: 4409648-4408689 (Counterclockwise)

Preceding gene: 49187489

Following gene: 49187487

Centisome position: 84.34

GC content: 39.48

Gene sequence:

>960_bases
ATGAAACGTATTGGTGTATTAACAAGTGGTGGAGATTCACCTGGTATGAATGCTGCCATTCGTGCAGTTGTTCGTAAAGC
GATTTTCCATGATATTGAAGTATATGGTATTTACCATGGATACGCTGGATTAATTTCTGGTCACATTGAAAAATTAGAAC
TAGGTTCTGTTGGCGATATTATCCACCGCGGTGGTACGAAATTATATACAGCAAGATGTCCTGAGTTTAAAGACCCAGAA
GTACGACTAAAAGGTATCGAGCAATTAAAGAAACACGGTATCGAAGGACTTGTTGTTATTGGTGGAGATGGTTCTTACCA
AGGCGCTAAAAAATTAACTGAACAAGGATTCCCTTGTGTTGGTGTACCAGGTACAATTGACAATGATATCCCTGGAACAG
ACTTCACAATTGGTTTCGATACAGCTTTAAACACTGTTATTGATGCAATTGATAAAATTCGTGACACAGCTACATCTCAT
GAACGTACATATGTTATCGAAGTAATGGGACGTCACGCTGGTGATATCGCATTATGGGCTGGTTTAGCTGATGGTGCAGA
AACAATCTTAATTCCAGAAGAAGAGTATGACATGGAAGATGTTATCGCTCGTCTGAAGCGCGGTAGTGAACGTGGTAAAA
AACACAGTATTATCGTTGTAGCTGAAGGTGTTGGAAGTGCAATTGACATCGGTAAGCACATTGAAGAAGCAACAAACTTT
GATACTCGCGTAACTGTATTAGGTCACGTACAACGTGGTGGATCACCAAGTGCACAAGACCGTGTATTAGCAAGCCGTCT
TGGCGCAAGAGCAGTTGAATTATTAATTGCTGGTAAAGGTGGACGTTGTGTAGGTATTCAAGATAACAAACTTGTTGATC
ATGACATTATCGAAGCGTTAGCTCAAAAGCATACAATCGATAAAGATATGTATCAATTATCTAAAGAATTATCCATCTAA

Upstream 100 bases:

>100_bases
CACGTTTTTATTATGAAAAGACACATCTTCTCCATTGTATTTTTATATTTTTTCGTGTTATTTTATTATAAGGCAAATAA
TCTTTATGAGGTGAGTACAA

Downstream 100 bases:

>100_bases
TCGGCGTAATGTCGGATATTTGGGAACGGTTTCATAATTTTCGGAGGTGCAATATGCGTAAAACTAAAATTGTATGTACT
ATAGGTCCTGCTAGTGAAAG

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKRIGVLTSGGDSPGMNAAIRAVVRKAIFHDIEVYGIYHGYAGLISGHIEKLELGSVGDIIHRGGTKLYTARCPEFKDPE
VRLKGIEQLKKHGIEGLVVIGGDGSYQGAKKLTEQGFPCVGVPGTIDNDIPGTDFTIGFDTALNTVIDAIDKIRDTATSH
ERTYVIEVMGRHAGDIALWAGLADGAETILIPEEEYDMEDVIARLKRGSERGKKHSIIVVAEGVGSAIDIGKHIEEATNF
DTRVTVLGHVQRGGSPSAQDRVLASRLGARAVELLIAGKGGRCVGIQDNKLVDHDIIEALAQKHTIDKDMYQLSKELSI

Sequences:

>Translated_319_residues
MKRIGVLTSGGDSPGMNAAIRAVVRKAIFHDIEVYGIYHGYAGLISGHIEKLELGSVGDIIHRGGTKLYTARCPEFKDPE
VRLKGIEQLKKHGIEGLVVIGGDGSYQGAKKLTEQGFPCVGVPGTIDNDIPGTDFTIGFDTALNTVIDAIDKIRDTATSH
ERTYVIEVMGRHAGDIALWAGLADGAETILIPEEEYDMEDVIARLKRGSERGKKHSIIVVAEGVGSAIDIGKHIEEATNF
DTRVTVLGHVQRGGSPSAQDRVLASRLGARAVELLIAGKGGRCVGIQDNKLVDHDIIEALAQKHTIDKDMYQLSKELSI
>Mature_319_residues
MKRIGVLTSGGDSPGMNAAIRAVVRKAIFHDIEVYGIYHGYAGLISGHIEKLELGSVGDIIHRGGTKLYTARCPEFKDPE
VRLKGIEQLKKHGIEGLVVIGGDGSYQGAKKLTEQGFPCVGVPGTIDNDIPGTDFTIGFDTALNTVIDAIDKIRDTATSH
ERTYVIEVMGRHAGDIALWAGLADGAETILIPEEEYDMEDVIARLKRGSERGKKHSIIVVAEGVGSAIDIGKHIEEATNF
DTRVTVLGHVQRGGSPSAQDRVLASRLGARAVELLIAGKGGRCVGIQDNKLVDHDIIEALAQKHTIDKDMYQLSKELSI

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family

Homologues:

Organism=Homo sapiens, GI11321601, Length=350, Percent_Identity=42.2857142857143, Blast_Score=231, Evalue=7e-61,
Organism=Homo sapiens, GI266453768, Length=347, Percent_Identity=42.0749279538905, Blast_Score=229, Evalue=2e-60,
Organism=Homo sapiens, GI266453748, Length=347, Percent_Identity=42.0749279538905, Blast_Score=229, Evalue=2e-60,
Organism=Homo sapiens, GI4505749, Length=347, Percent_Identity=42.0749279538905, Blast_Score=229, Evalue=2e-60,
Organism=Homo sapiens, GI266453619, Length=347, Percent_Identity=42.0749279538905, Blast_Score=229, Evalue=3e-60,
Organism=Homo sapiens, GI48762920, Length=350, Percent_Identity=42.2857142857143, Blast_Score=225, Evalue=4e-59,
Organism=Escherichia coli, GI1790350, Length=318, Percent_Identity=55.9748427672956, Blast_Score=360, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI25147584, Length=347, Percent_Identity=40.9221902017291, Blast_Score=224, Evalue=4e-59,
Organism=Caenorhabditis elegans, GI17558788, Length=309, Percent_Identity=39.4822006472492, Blast_Score=200, Evalue=9e-52,
Organism=Caenorhabditis elegans, GI72003601, Length=319, Percent_Identity=32.9153605015674, Blast_Score=172, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6323861, Length=343, Percent_Identity=37.6093294460641, Blast_Score=204, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6321679, Length=338, Percent_Identity=38.7573964497041, Blast_Score=203, Evalue=3e-53,
Organism=Drosophila melanogaster, GI28573326, Length=347, Percent_Identity=41.4985590778098, Blast_Score=237, Evalue=9e-63,
Organism=Drosophila melanogaster, GI24652337, Length=347, Percent_Identity=42.6512968299712, Blast_Score=236, Evalue=1e-62,
Organism=Drosophila melanogaster, GI17647809, Length=347, Percent_Identity=42.6512968299712, Blast_Score=236, Evalue=2e-62,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): K6PF_BACAA (C3PAI8)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002868878.1
- ProteinModelPortal:   C3PAI8
- SMR:   C3PAI8
- EnsemblBacteria:   EBBACT00000128608
- GeneID:   7850067
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_4855
- GeneTree:   EBGT00050000001727
- ProtClustDB:   PRK03202
- GO:   GO:0006096
- HAMAP:   MF_00339
- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023
- PIRSF:   PIRSF000532
- PRINTS:   PR00476
- TIGRFAMs:   TIGR02482

Pfam domain/function: PF00365 PFK; SSF53784 Ppfruckinase

EC number: =2.7.1.11

Molecular weight: Translated: 34309; Mature: 34309

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: ACT_SITE 127-127 BINDING 162-162 BINDING 243-243 BINDING 249-249 BINDING 252-252

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIGVLTSGGDSPGMNAAIRAVVRKAIFHDIEVYGIYHGYAGLISGHIEKLELGSVGDI
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHEEEEEEHHHHHHHHCCHHHEECCCHHHH
IHRGGTKLYTARCPEFKDPEVRLKGIEQLKKHGIEGLVVIGGDGSYQGAKKLTEQGFPCV
HHCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCEE
GVPGTIDNDIPGTDFTIGFDTALNTVIDAIDKIRDTATSHERTYVIEVMGRHAGDIALWA
CCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEE
GLADGAETILIPEEEYDMEDVIARLKRGSERGKKHSIIVVAEGVGSAIDIGKHIEEATNF
ECCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCC
DTRVTVLGHVQRGGSPSAQDRVLASRLGARAVELLIAGKGGRCVGIQDNKLVDHDIIEAL
CCEEEEEEEECCCCCCCHHHHHHHHHHHHHEEEEEEECCCCEEECCCCCCCCHHHHHHHH
AQKHTIDKDMYQLSKELSI
HHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKRIGVLTSGGDSPGMNAAIRAVVRKAIFHDIEVYGIYHGYAGLISGHIEKLELGSVGDI
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHEEEEEEHHHHHHHHCCHHHEECCCHHHH
IHRGGTKLYTARCPEFKDPEVRLKGIEQLKKHGIEGLVVIGGDGSYQGAKKLTEQGFPCV
HHCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCEE
GVPGTIDNDIPGTDFTIGFDTALNTVIDAIDKIRDTATSHERTYVIEVMGRHAGDIALWA
CCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEE
GLADGAETILIPEEEYDMEDVIARLKRGSERGKKHSIIVVAEGVGSAIDIGKHIEEATNF
ECCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCC
DTRVTVLGHVQRGGSPSAQDRVLASRLGARAVELLIAGKGGRCVGIQDNKLVDHDIIEAL
CCEEEEEEEECCCCCCCHHHHHHHHHHHHHEEEEEEECCCCEEECCCCCCCCHHHHHHHH
AQKHTIDKDMYQLSKELSI
HHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA