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The map label for this gene is pyk [H]

Identifier: 49187487

GI number: 49187487

Start: 4406878

End: 4408635

Strand: Reverse

Name: pyk [H]

Synonym: BAS4492

Alternate gene names: 49187487

Gene position: 4408635-4406878 (Counterclockwise)

Preceding gene: 49187488

Following gene: 49187486

Centisome position: 84.32

GC content: 40.44

Gene sequence:

>1758_bases
ATGCGTAAAACTAAAATTGTATGTACTATAGGTCCTGCTAGTGAAAGTATTGAAAAATTAGAGCAATTAATCGAAGCAGG
TATGAACGTTGCTCGTTTAAACTTCTCTCATGGTAGCCATGAAGAGCACGGTGCTCGTATTAAAAACATTCGTGAAGCTT
CAAAGAAAACTGGTAAAACAGTTGGTATCTTACTTGATACAAAAGGTCCAGAAATCCGTACTCATGACTTCGTAGACGGA
CAAGCTGAGCTTGTAACAGGTGCAGAAGTAGTTCTTTCTACTGAGCAAGTATTAGGTACTGCAGAGAAGTTCTCTGTATC
TTATGCTGGTCTTTATGACGATGTAGATCCAGGTTCTCGCATTCTAATCGATGACGGTCTTATCGAGCTAGAAGTAATCG
AAAAAGCTGACGGAAACATCCGTACAAAAGTTCTTAACAGCGGAACTGTAAAAAATAAAAAAGGTGTTAACGTACCAAAC
GTAAGCATTAAGCTTCCTGGTATCACTGAAAAAGACGTAAAAGATATCATCTTCGGTATCGAGCAAAAAGTTGATTTCAT
CGCAGCTTCATTCGTACGTAAAGCAGCTGACGTATTAGAAATCCGTGAATTATTAGAAGAGCATAACGCTCAATACATCC
AAATCGTACCAAAAATTGAAAACCAAGAGGGTATCGACAACATCGATTCAATCTTAGAAGTTTCTGACGGCTTAATGGTA
GCTCGTGGTGACATGGGTGTAGAAATTCCACCAGAAGAAGTACCATTAGTACAAAAACGTCTAATCAAAAAATGTAACGT
GTTAGGTAAACCAGTTATTACTGCAACACAAATGTTAGATTCTATGCAACGTAACCCACGTCCAACTCGTGCGGAAGCAA
GTGACGTAGCTAACGCAATCTTCGATGGTACAGATGCAATCATGCTTTCAGGTGAAACGGCTGCTGGACAATACCCAGTA
GAAGCAGTAACAATGATGGCTAACATTGCAGTACGTGTTGAAAAATCATTACAATATGAAGATATGTTCAAAAAACGTAT
TAAAGAGTTCACTCCAACAATTACAGATGCAATTAGCCAATCTGTTGCGCATACAGCACTTGCTCTTGATGTAGCTGCAA
TCGTAGCTCCAACAGAAAGTGGATATACTGCGAAAATGATCTCTAAATACCGTCCAAAATCTCCAATCGTAGCTGTAACA
TCTGATGAGCAAGTAGGACGTCGTCTTGCACTTGTTTGGGGTGTACAAGCATTTATGGCTGAGAAGCGTGCGGCTTCTAC
TGACGAAATGTTAGATACAGCGATTCAAACAGGTATGGATGCAGGTCTAATCGGACTTGGAGACACTGTAGTAATCACTG
CTGGTGTTCCAGTTGCTGAAACTGGTACAACAAACTTAATGAAAATCCACGTTGTTGGTGAAGAAGTTGCTAAAGGACAA
GGAATCGGTCGTAAAGCTGCAAAAGGTAAAGTAGTTGTAGCGAAAACAGCTGCTGAAGCTGTAGCGAACGTAAACGAAGG
TGATATCCTTGTTACAACAAGCACTGATAAAGATATGATTCCTGCAATCGAAAAAGCTGCTGCTTTAGTTGTAGAAGAAG
GCGGCCTAACAAGCCATGCGGCTGTTGTAGGTGTATCAATCGGTATTCCTGTTATCGTTGGTGTAAACGGCGTAACAGCA
ACTTTAAAAAATGGCCAAGAAGTAACAGTTGATGCAGCACGCGGAATTGTTTATAATGGACATGCGGAAGTGCTATAA

Upstream 100 bases:

>100_bases
CAATCGATAAAGATATGTATCAATTATCTAAAGAATTATCCATCTAATCGGCGTAATGTCGGATATTTGGGAACGGTTTC
ATAATTTTCGGAGGTGCAAT

Downstream 100 bases:

>100_bases
GTAATACGGAGAGAAGGATCGGAGTCCTTCTCTTTTTTTTACACAAAAAAAGAGATAGAACTAGCTTGAGGTTCCTGAAA
TTGCTTCTAAAAGCTGTCTA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK; Vegetative protein 17; VEG17 [H]

Number of amino acids: Translated: 585; Mature: 585

Protein sequence:

>585_residues
MRKTKIVCTIGPASESIEKLEQLIEAGMNVARLNFSHGSHEEHGARIKNIREASKKTGKTVGILLDTKGPEIRTHDFVDG
QAELVTGAEVVLSTEQVLGTAEKFSVSYAGLYDDVDPGSRILIDDGLIELEVIEKADGNIRTKVLNSGTVKNKKGVNVPN
VSIKLPGITEKDVKDIIFGIEQKVDFIAASFVRKAADVLEIRELLEEHNAQYIQIVPKIENQEGIDNIDSILEVSDGLMV
ARGDMGVEIPPEEVPLVQKRLIKKCNVLGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAIMLSGETAAGQYPV
EAVTMMANIAVRVEKSLQYEDMFKKRIKEFTPTITDAISQSVAHTALALDVAAIVAPTESGYTAKMISKYRPKSPIVAVT
SDEQVGRRLALVWGVQAFMAEKRAASTDEMLDTAIQTGMDAGLIGLGDTVVITAGVPVAETGTTNLMKIHVVGEEVAKGQ
GIGRKAAKGKVVVAKTAAEAVANVNEGDILVTTSTDKDMIPAIEKAAALVVEEGGLTSHAAVVGVSIGIPVIVGVNGVTA
TLKNGQEVTVDAARGIVYNGHAEVL

Sequences:

>Translated_585_residues
MRKTKIVCTIGPASESIEKLEQLIEAGMNVARLNFSHGSHEEHGARIKNIREASKKTGKTVGILLDTKGPEIRTHDFVDG
QAELVTGAEVVLSTEQVLGTAEKFSVSYAGLYDDVDPGSRILIDDGLIELEVIEKADGNIRTKVLNSGTVKNKKGVNVPN
VSIKLPGITEKDVKDIIFGIEQKVDFIAASFVRKAADVLEIRELLEEHNAQYIQIVPKIENQEGIDNIDSILEVSDGLMV
ARGDMGVEIPPEEVPLVQKRLIKKCNVLGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAIMLSGETAAGQYPV
EAVTMMANIAVRVEKSLQYEDMFKKRIKEFTPTITDAISQSVAHTALALDVAAIVAPTESGYTAKMISKYRPKSPIVAVT
SDEQVGRRLALVWGVQAFMAEKRAASTDEMLDTAIQTGMDAGLIGLGDTVVITAGVPVAETGTTNLMKIHVVGEEVAKGQ
GIGRKAAKGKVVVAKTAAEAVANVNEGDILVTTSTDKDMIPAIEKAAALVVEEGGLTSHAAVVGVSIGIPVIVGVNGVTA
TLKNGQEVTVDAARGIVYNGHAEVL
>Mature_585_residues
MRKTKIVCTIGPASESIEKLEQLIEAGMNVARLNFSHGSHEEHGARIKNIREASKKTGKTVGILLDTKGPEIRTHDFVDG
QAELVTGAEVVLSTEQVLGTAEKFSVSYAGLYDDVDPGSRILIDDGLIELEVIEKADGNIRTKVLNSGTVKNKKGVNVPN
VSIKLPGITEKDVKDIIFGIEQKVDFIAASFVRKAADVLEIRELLEEHNAQYIQIVPKIENQEGIDNIDSILEVSDGLMV
ARGDMGVEIPPEEVPLVQKRLIKKCNVLGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAIMLSGETAAGQYPV
EAVTMMANIAVRVEKSLQYEDMFKKRIKEFTPTITDAISQSVAHTALALDVAAIVAPTESGYTAKMISKYRPKSPIVAVT
SDEQVGRRLALVWGVQAFMAEKRAASTDEMLDTAIQTGMDAGLIGLGDTVVITAGVPVAETGTTNLMKIHVVGEEVAKGQ
GIGRKAAKGKVVVAKTAAEAVANVNEGDILVTTSTDKDMIPAIEKAAALVVEEGGLTSHAAVVGVSIGIPVIVGVNGVTA
TLKNGQEVTVDAARGIVYNGHAEVL

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI10835121, Length=489, Percent_Identity=44.3762781186094, Blast_Score=394, Evalue=1e-109,
Organism=Homo sapiens, GI32967597, Length=489, Percent_Identity=44.3762781186094, Blast_Score=394, Evalue=1e-109,
Organism=Homo sapiens, GI33286422, Length=490, Percent_Identity=43.6734693877551, Blast_Score=394, Evalue=1e-109,
Organism=Homo sapiens, GI33286420, Length=490, Percent_Identity=43.6734693877551, Blast_Score=394, Evalue=1e-109,
Organism=Homo sapiens, GI33286418, Length=490, Percent_Identity=43.8775510204082, Blast_Score=393, Evalue=1e-109,
Organism=Homo sapiens, GI310128732, Length=291, Percent_Identity=47.766323024055, Blast_Score=257, Evalue=2e-68,
Organism=Homo sapiens, GI310128730, Length=291, Percent_Identity=47.766323024055, Blast_Score=257, Evalue=2e-68,
Organism=Homo sapiens, GI310128736, Length=239, Percent_Identity=44.7698744769874, Blast_Score=196, Evalue=4e-50,
Organism=Homo sapiens, GI310128734, Length=239, Percent_Identity=44.7698744769874, Blast_Score=196, Evalue=4e-50,
Organism=Homo sapiens, GI310128738, Length=218, Percent_Identity=43.1192660550459, Blast_Score=166, Evalue=4e-41,
Organism=Escherichia coli, GI1787965, Length=474, Percent_Identity=50.210970464135, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI1788160, Length=481, Percent_Identity=40.1247401247401, Blast_Score=295, Evalue=4e-81,
Organism=Escherichia coli, GI1787994, Length=117, Percent_Identity=38.4615384615385, Blast_Score=83, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17544584, Length=480, Percent_Identity=44.7916666666667, Blast_Score=393, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI71984413, Length=484, Percent_Identity=45.6611570247934, Blast_Score=366, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17506829, Length=484, Percent_Identity=45.6611570247934, Blast_Score=366, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI71984406, Length=484, Percent_Identity=45.6611570247934, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17506831, Length=484, Percent_Identity=45.4545454545455, Blast_Score=365, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6324923, Length=490, Percent_Identity=41.4285714285714, Blast_Score=361, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6319279, Length=486, Percent_Identity=41.5637860082305, Blast_Score=350, Evalue=3e-97,
Organism=Drosophila melanogaster, GI24648964, Length=488, Percent_Identity=44.0573770491803, Blast_Score=387, Evalue=1e-107,
Organism=Drosophila melanogaster, GI28571814, Length=488, Percent_Identity=44.0573770491803, Blast_Score=387, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24648966, Length=410, Percent_Identity=47.0731707317073, Blast_Score=348, Evalue=8e-96,
Organism=Drosophila melanogaster, GI24581235, Length=489, Percent_Identity=35.3783231083845, Blast_Score=289, Evalue=3e-78,
Organism=Drosophila melanogaster, GI24646914, Length=306, Percent_Identity=41.5032679738562, Blast_Score=214, Evalue=2e-55,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 62238; Mature: 62238

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKTKIVCTIGPASESIEKLEQLIEAGMNVARLNFSHGSHEEHGARIKNIREASKKTGKT
CCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCE
VGILLDTKGPEIRTHDFVDGQAELVTGAEVVLSTEQVLGTAEKFSVSYAGLYDDVDPGSR
EEEEEECCCCCCEECCCCCCCCEEEECHHHEEEHHHHHCCHHHHEEEEECCCCCCCCCCE
ILIDDGLIELEVIEKADGNIRTKVLNSGTVKNKKGVNVPNVSIKLPGITEKDVKDIIFGI
EEEECCEEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHH
EQKVDFIAASFVRKAADVLEIRELLEEHNAQYIQIVPKIENQEGIDNIDSILEVSDGLMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHCCCEEE
ARGDMGVEIPPEEVPLVQKRLIKKCNVLGKPVITATQMLDSMQRNPRPTRAEASDVANAI
EECCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
FDGTDAIMLSGETAAGQYPVEAVTMMANIAVRVEKSLQYEDMFKKRIKEFTPTITDAISQ
HCCCCEEEEECCCCCCCCCHHHHHHHHHHHEEHHHCCCHHHHHHHHHHHHCCHHHHHHHH
SVAHTALALDVAAIVAPTESGYTAKMISKYRPKSPIVAVTSDEQVGRRLALVWGVQAFMA
HHHHHHHHHHHHHEEECCCCCCHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHH
EKRAASTDEMLDTAIQTGMDAGLIGLGDTVVITAGVPVAETGTTNLMKIHVVGEEVAKGQ
HHHCCCHHHHHHHHHHCCCCCCEEECCCEEEEEECCCEECCCCCCEEEEEEECHHHHCCC
GIGRKAAKGKVVVAKTAAEAVANVNEGDILVTTSTDKDMIPAIEKAAALVVEEGGLTSHA
CCCCCCCCCCEEEEECHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHEEEEECCCCCCCC
AVVGVSIGIPVIVGVNGVTATLKNGQEVTVDAARGIVYNGHAEVL
EEEEEEECCEEEEECCCEEEEECCCCEEEEEECCCEEECCCCCCC
>Mature Secondary Structure
MRKTKIVCTIGPASESIEKLEQLIEAGMNVARLNFSHGSHEEHGARIKNIREASKKTGKT
CCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCE
VGILLDTKGPEIRTHDFVDGQAELVTGAEVVLSTEQVLGTAEKFSVSYAGLYDDVDPGSR
EEEEEECCCCCCEECCCCCCCCEEEECHHHEEEHHHHHCCHHHHEEEEECCCCCCCCCCE
ILIDDGLIELEVIEKADGNIRTKVLNSGTVKNKKGVNVPNVSIKLPGITEKDVKDIIFGI
EEEECCEEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHH
EQKVDFIAASFVRKAADVLEIRELLEEHNAQYIQIVPKIENQEGIDNIDSILEVSDGLMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHCCCEEE
ARGDMGVEIPPEEVPLVQKRLIKKCNVLGKPVITATQMLDSMQRNPRPTRAEASDVANAI
EECCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
FDGTDAIMLSGETAAGQYPVEAVTMMANIAVRVEKSLQYEDMFKKRIKEFTPTITDAISQ
HCCCCEEEEECCCCCCCCCHHHHHHHHHHHEEHHHCCCHHHHHHHHHHHHCCHHHHHHHH
SVAHTALALDVAAIVAPTESGYTAKMISKYRPKSPIVAVTSDEQVGRRLALVWGVQAFMA
HHHHHHHHHHHHHEEECCCCCCHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHH
EKRAASTDEMLDTAIQTGMDAGLIGLGDTVVITAGVPVAETGTTNLMKIHVVGEEVAKGQ
HHHCCCHHHHHHHHHHCCCCCCEEECCCEEEEEECCCEECCCCCCEEEEEEECHHHHCCC
GIGRKAAKGKVVVAKTAAEAVANVNEGDILVTTSTDKDMIPAIEKAAALVVEEGGLTSHA
CCCCCCCCCCEEEEECHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHEEEEECCCCCCCC
AVVGVSIGIPVIVGVNGVTATLKNGQEVTVDAARGIVYNGHAEVL
EEEEEEECCEEEEECCCEEEEECCCCEEEEEECCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 9298659 [H]