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The map label for this gene is sigA [H]

Identifier: 49187190

GI number: 49187190

Start: 4108719

End: 4109840

Strand: Reverse

Name: sigA [H]

Synonym: BAS4194

Alternate gene names: 49187190

Gene position: 4109840-4108719 (Counterclockwise)

Preceding gene: 49187191

Following gene: 49187189

Centisome position: 78.6

GC content: 39.39

Gene sequence:

>1122_bases
ATGGCTGACAAACCAGCTCGTTCTAAACAAATTGAAACTGAAATGACCCTTGAGCAAGTGAAAGAACAACTCACTGAGCT
CGGAAAAAAACGTGGCGTTCTTACATATGAAGAGATTGCAGAACGCATGAATGGATTTGAAATTGAATCCGATCAAATGG
ATGAATACTATGAATATTTAGGTGAACAAGGGATTGACTTAGTTGGCGACAACGATGAAGGCCCTAATAATCACCAAATT
ACGAAAACAGAAGAAGAGTTTGACCTAAATGACTTAAGTGTACCACCAGGGGTTAAAATCAACGATCCTGTTCGTATGTA
TTTAAAAGAAATTGGTCGTGTAGATTTACTATCTGCAGAAGAAGAAATTCGACTTGCAACGCGTATTGAAGAAGGCGATG
AAGAAGCAAAACGTCGTCTTGCAGAAGCAAACTTACGTCTTGTAGTAAGTATTGCAAAGCGCTACGTAGGTCGCGGTATG
CTTTTCTTAGACTTAATCCAAGAAGGGAATATGGGTCTAATTAAAGCGGTTGAAAAGTTCGATTATCGTAAAGGTTTCAA
ATTTAGTACGTATGCAACTTGGTGGATTCGCCAAGCAATTACACGTGCGATTGCAGACCAAGCAAGAACAATTCGTATCC
CAGTTCATATGGTTGAAACGATTAATAAGTTAATTCGTGTACAACGTCAATTATTACAAGATTTAGGACGTGAACCATCT
CCTGAAGAGATTGGTGAAGAAATGGATCTTGCTCCAGAAAAAGTGCGCGAAATCTTAAAAATTGCTCAGGAGCCAGTCTC
TCTTGAAACACCGATTGGTGAAGAAGATGACTCCCATTTAGGTGATTTTATTGAAGACCAAGAAGCAACATCGCCTGCGG
ACCATGCAGCGTATGAATTGCTAAAAGAACAATTAGAAGATGTGTTAGATACACTAACAGATCGTGAAGAAAATGTTCTA
CGTCTTCGTTTTGGTTTAGATGATGGACGAACTCGTACGCTTGAAGAAGTTGGGAAAGTATTCGGCGTAACGAGAGAACG
TATTCGTCAAATTGAAGCAAAAGCACTTCGTAAATTGAGACATCCTAGCCGTAGTAAGCGTCTTAAGGATTTCTTAGAAT
AG

Upstream 100 bases:

>100_bases
ATTATGTAGCATATTTACAAAATCAAAAAGCGAGAAAATAGCTTTTTTGATAAATTTTTAGTATAATGAATGTTTGTATC
TCGCGTAAGGAGGGGAACAG

Downstream 100 bases:

>100_bases
GCTACCTTTAAGTTTACTTCACCTTTGTGAAGTAAACTTTTTTATTTTGTTAATTTTTGTTAAACTTTAGCTGTCAGCAA
CGATTACAATTTCTTGTAAT

Product: RNA polymerase sigma factor RpoD

Products: NA

Alternate protein names: Sigma-43; Sigma-A [H]

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MADKPARSKQIETEMTLEQVKEQLTELGKKRGVLTYEEIAERMNGFEIESDQMDEYYEYLGEQGIDLVGDNDEGPNNHQI
TKTEEEFDLNDLSVPPGVKINDPVRMYLKEIGRVDLLSAEEEIRLATRIEEGDEEAKRRLAEANLRLVVSIAKRYVGRGM
LFLDLIQEGNMGLIKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQDLGREPS
PEEIGEEMDLAPEKVREILKIAQEPVSLETPIGEEDDSHLGDFIEDQEATSPADHAAYELLKEQLEDVLDTLTDREENVL
RLRFGLDDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLRHPSRSKRLKDFLE

Sequences:

>Translated_373_residues
MADKPARSKQIETEMTLEQVKEQLTELGKKRGVLTYEEIAERMNGFEIESDQMDEYYEYLGEQGIDLVGDNDEGPNNHQI
TKTEEEFDLNDLSVPPGVKINDPVRMYLKEIGRVDLLSAEEEIRLATRIEEGDEEAKRRLAEANLRLVVSIAKRYVGRGM
LFLDLIQEGNMGLIKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQDLGREPS
PEEIGEEMDLAPEKVREILKIAQEPVSLETPIGEEDDSHLGDFIEDQEATSPADHAAYELLKEQLEDVLDTLTDREENVL
RLRFGLDDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLRHPSRSKRLKDFLE
>Mature_372_residues
ADKPARSKQIETEMTLEQVKEQLTELGKKRGVLTYEEIAERMNGFEIESDQMDEYYEYLGEQGIDLVGDNDEGPNNHQIT
KTEEEFDLNDLSVPPGVKINDPVRMYLKEIGRVDLLSAEEEIRLATRIEEGDEEAKRRLAEANLRLVVSIAKRYVGRGML
FLDLIQEGNMGLIKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQDLGREPSP
EEIGEEMDLAPEKVREILKIAQEPVSLETPIGEEDDSHLGDFIEDQEATSPADHAAYELLKEQLEDVLDTLTDREENVLR
LRFGLDDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLRHPSRSKRLKDFLE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=238, Percent_Identity=68.0672268907563, Blast_Score=342, Evalue=3e-95,
Organism=Escherichia coli, GI1789098, Length=269, Percent_Identity=46.0966542750929, Blast_Score=239, Evalue=3e-64,
Organism=Escherichia coli, GI1789871, Length=272, Percent_Identity=30.1470588235294, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788231, Length=202, Percent_Identity=28.7128712871287, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 43038; Mature: 42907

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADKPARSKQIETEMTLEQVKEQLTELGKKRGVLTYEEIAERMNGFEIESDQMDEYYEYL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHH
GEQGIDLVGDNDEGPNNHQITKTEEEFDLNDLSVPPGVKINDPVRMYLKEIGRVDLLSAE
HHCCCEEECCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEECCH
EEIRLATRIEEGDEEAKRRLAEANLRLVVSIAKRYVGRGMLFLDLIQEGNMGLIKAVEKF
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHH
DYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQDLGREPS
HHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCC
PEEIGEEMDLAPEKVREILKIAQEPVSLETPIGEEDDSHLGDFIEDQEATSPADHAAYEL
HHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHH
LKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLR
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HPSRSKRLKDFLE
CCHHHHHHHHHHC
>Mature Secondary Structure 
ADKPARSKQIETEMTLEQVKEQLTELGKKRGVLTYEEIAERMNGFEIESDQMDEYYEYL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHH
GEQGIDLVGDNDEGPNNHQITKTEEEFDLNDLSVPPGVKINDPVRMYLKEIGRVDLLSAE
HHCCCEEECCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEECCH
EEIRLATRIEEGDEEAKRRLAEANLRLVVSIAKRYVGRGMLFLDLIQEGNMGLIKAVEKF
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHH
DYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQDLGREPS
HHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCC
PEEIGEEMDLAPEKVREILKIAQEPVSLETPIGEEDDSHLGDFIEDQEATSPADHAAYEL
HHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHH
LKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLR
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HPSRSKRLKDFLE
CCHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987246; 3086839; 8969508; 9384377; 2166045; 8363585 [H]