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The map label for this gene is dnaG [H]

Identifier: 49187191

GI number: 49187191

Start: 4109900

End: 4111696

Strand: Reverse

Name: dnaG [H]

Synonym: BAS4195

Alternate gene names: 49187191

Gene position: 4111696-4109900 (Counterclockwise)

Preceding gene: 49187192

Following gene: 49187190

Centisome position: 78.64

GC content: 37.17

Gene sequence:

>1797_bases
ATGGGGAACAGAATTCCTGAAGAAGTTGTTGAACAGATTCGGACGTCATCCGATATTGTCGAAGTGATTGGTGAATACGT
TCAACTTAGAAAACAGGGACGTAACTATTTCGGCCTTTGTCCGTTTCATGGTGAGAATTCTCCTTCATTCTCTGTTTCAT
CTGATAAGCAAATTTTTCATTGCTTCGGATGTGGAGAAGGTGGAAATGTCTTTTCCTTTCTAATGAAAATGGAAGGACTG
GCTTTTACCGAGGCTGTTCAAAAGCTAGGTGAAAGAAATGGAATTGCGGTTGCAGAGTATACATCAGGGCAAGGACAACA
AGAAGACATATCTGATGACACTGTCATCATGCAACAGGCTCATGAACTTTTGAAAAAGTATTATCATCATTTATTAGTAA
ATACAGAAGAAGGAAATGAAGCACTTTCGTATTTGTTAAAACGTGGTATTACAAAAGAAATGATTGAGAAGTTTGAAATT
GGTTATGCGTCACCTGCTTGGGATGCAGCAACGAAAATTTTACAAAAAAGAGGTTTATCGCTGTCTAGTATGGAACAAGC
TGGTCTTCTCATAAGAAGTGAGAAGGATGGGAGCCATTATGATCGCTTCCGTGGACGAGTTATGTTTCCAATCTATACGT
TACAAGGTAAAGTGATAGCGTTTAGTGGAAGGGCATTAGGAGATGATACTCCGAAATATTTAAATAGCCCAGAAACACCG
ATTTTTCACAAAAGTAAATTGTTGTATAACTTCCACCAAGCGAGGCCGTTTATTAGAAAACGTGGGCAAGTGGTCCTTTT
TGAAGGATATGCCGACGTACTAGCTGCGGTAAAAAGTGGTGTGGAAGAAGCTGTTGCGACAATGGGAACAGCTTTAACTG
AAGAACAAGCAAAACTTCTGCGACGTAACGTTGAAACTGTTGTTCTTTGCTATGATGGTGATAAAGCAGGGCGAGAAGCG
ACGATGAAAGCAGGGCAATTATTGTTGCAAGTTGGTTGCCAAGTAAAAGTTACATCCTTGCCAGATAAGCTTGATCCTGA
TGAATATGTGCAACAATATGGGACAACTGCTTTTGAAAATCTTGTGAAATCAAGTATAAGTTTTGTTGGTTTTAAAATAA
ATTATTTGCGTTTAGGGAAAAATTTGCAAGATGAGTCGGGCAAAGAAGAGTATGTGAAAAGTGTTTTAAAAGAGTTATCG
TTGTTACAAGATGCGATGCAGGCAGAATCATATTTGAAGTCATTATCGCAAGAATTTTCGTATTCAATGGAAACACTTTT
GAACCAATTGCACCAATATCGCAAAGAACAAAAGGTACAGCAAAAACAAGTAAAGCAGGTTTCTAAGCCGTCTCAAATTG
TTCAAACAAAACCGAAATTAACAGGTTTTGAAAGGGCAGAAAGAGAAATTATTTACCATATGTTGCAAAGTCCAGAAGTG
GCCGTTCGTATGGAATCCCACATAGAAGATTTTCATACAGAAGAACATAAAGGGATTTTATATGAACTATACGCATATTA
TGAAAAGGGAAATGAACCTTCAGTCGGAACATTTTTAAGTTGGCTCTCTGATGAAAAGTTGAAAAATATTATTACCGATA
TTTCGACGGATGAATTTATTAATCCAGAATACACAGAAGAAGTGTTACAAAGCCATTTGGAGACGCTTAGACGTCATCAA
GAAAAACTTGAAAAGATGGAAATCATCTTCAAGATAAAACAAATGGAAAAAACAGATCCTGTAGAGGCTGCTAAATATTA
TGTAGCATATTTACAAAATCAAAAAGCGAGAAAATAG

Upstream 100 bases:

>100_bases
ATCGATAACATTTTCTAATAAGTTTAGAAAAAATATTGTCGAATTATAAAGGAATTTTTTAATTTATATCGAATACAAAA
TACGACACGGAGATGGAGTT

Downstream 100 bases:

>100_bases
CTTTTTTGATAAATTTTTAGTATAATGAATGTTTGTATCTCGCGTAAGGAGGGGAACAGATGGCTGACAAACCAGCTCGT
TCTAAACAAATTGAAACTGA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 598; Mature: 597

Protein sequence:

>598_residues
MGNRIPEEVVEQIRTSSDIVEVIGEYVQLRKQGRNYFGLCPFHGENSPSFSVSSDKQIFHCFGCGEGGNVFSFLMKMEGL
AFTEAVQKLGERNGIAVAEYTSGQGQQEDISDDTVIMQQAHELLKKYYHHLLVNTEEGNEALSYLLKRGITKEMIEKFEI
GYASPAWDAATKILQKRGLSLSSMEQAGLLIRSEKDGSHYDRFRGRVMFPIYTLQGKVIAFSGRALGDDTPKYLNSPETP
IFHKSKLLYNFHQARPFIRKRGQVVLFEGYADVLAAVKSGVEEAVATMGTALTEEQAKLLRRNVETVVLCYDGDKAGREA
TMKAGQLLLQVGCQVKVTSLPDKLDPDEYVQQYGTTAFENLVKSSISFVGFKINYLRLGKNLQDESGKEEYVKSVLKELS
LLQDAMQAESYLKSLSQEFSYSMETLLNQLHQYRKEQKVQQKQVKQVSKPSQIVQTKPKLTGFERAEREIIYHMLQSPEV
AVRMESHIEDFHTEEHKGILYELYAYYEKGNEPSVGTFLSWLSDEKLKNIITDISTDEFINPEYTEEVLQSHLETLRRHQ
EKLEKMEIIFKIKQMEKTDPVEAAKYYVAYLQNQKARK

Sequences:

>Translated_598_residues
MGNRIPEEVVEQIRTSSDIVEVIGEYVQLRKQGRNYFGLCPFHGENSPSFSVSSDKQIFHCFGCGEGGNVFSFLMKMEGL
AFTEAVQKLGERNGIAVAEYTSGQGQQEDISDDTVIMQQAHELLKKYYHHLLVNTEEGNEALSYLLKRGITKEMIEKFEI
GYASPAWDAATKILQKRGLSLSSMEQAGLLIRSEKDGSHYDRFRGRVMFPIYTLQGKVIAFSGRALGDDTPKYLNSPETP
IFHKSKLLYNFHQARPFIRKRGQVVLFEGYADVLAAVKSGVEEAVATMGTALTEEQAKLLRRNVETVVLCYDGDKAGREA
TMKAGQLLLQVGCQVKVTSLPDKLDPDEYVQQYGTTAFENLVKSSISFVGFKINYLRLGKNLQDESGKEEYVKSVLKELS
LLQDAMQAESYLKSLSQEFSYSMETLLNQLHQYRKEQKVQQKQVKQVSKPSQIVQTKPKLTGFERAEREIIYHMLQSPEV
AVRMESHIEDFHTEEHKGILYELYAYYEKGNEPSVGTFLSWLSDEKLKNIITDISTDEFINPEYTEEVLQSHLETLRRHQ
EKLEKMEIIFKIKQMEKTDPVEAAKYYVAYLQNQKARK
>Mature_597_residues
GNRIPEEVVEQIRTSSDIVEVIGEYVQLRKQGRNYFGLCPFHGENSPSFSVSSDKQIFHCFGCGEGGNVFSFLMKMEGLA
FTEAVQKLGERNGIAVAEYTSGQGQQEDISDDTVIMQQAHELLKKYYHHLLVNTEEGNEALSYLLKRGITKEMIEKFEIG
YASPAWDAATKILQKRGLSLSSMEQAGLLIRSEKDGSHYDRFRGRVMFPIYTLQGKVIAFSGRALGDDTPKYLNSPETPI
FHKSKLLYNFHQARPFIRKRGQVVLFEGYADVLAAVKSGVEEAVATMGTALTEEQAKLLRRNVETVVLCYDGDKAGREAT
MKAGQLLLQVGCQVKVTSLPDKLDPDEYVQQYGTTAFENLVKSSISFVGFKINYLRLGKNLQDESGKEEYVKSVLKELSL
LQDAMQAESYLKSLSQEFSYSMETLLNQLHQYRKEQKVQQKQVKQVSKPSQIVQTKPKLTGFERAEREIIYHMLQSPEVA
VRMESHIEDFHTEEHKGILYELYAYYEKGNEPSVGTFLSWLSDEKLKNIITDISTDEFINPEYTEEVLQSHLETLRRHQE
KLEKMEIIFKIKQMEKTDPVEAAKYYVAYLQNQKARK

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=485, Percent_Identity=35.4639175257732, Blast_Score=293, Evalue=2e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007693
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 68262; Mature: 68130

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNRIPEEVVEQIRTSSDIVEVIGEYVQLRKQGRNYFGLCPFHGENSPSFSVSSDKQIFH
CCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCEEE
CFGCGEGGNVFSFLMKMEGLAFTEAVQKLGERNGIAVAEYTSGQGQQEDISDDTVIMQQA
EEECCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHH
HELLKKYYHHLLVNTEEGNEALSYLLKRGITKEMIEKFEIGYASPAWDAATKILQKRGLS
HHHHHHHHHHHEECCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
LSSMEQAGLLIRSEKDGSHYDRFRGRVMFPIYTLQGKVIAFSGRALGDDTPKYLNSPETP
HHHHHHCCCEEEECCCCCHHHHHCCCEEEEEEEECCEEEEEECCCCCCCCHHHCCCCCCC
IFHKSKLLYNFHQARPFIRKRGQVVLFEGYADVLAAVKSGVEEAVATMGTALTEEQAKLL
CEEHHHHHHHHHHHCHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRNVETVVLCYDGDKAGREATMKAGQLLLQVGCQVKVTSLPDKLDPDEYVQQYGTTAFEN
HCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHCCHHHHH
LVKSSISFVGFKINYLRLGKNLQDESGKEEYVKSVLKELSLLQDAMQAESYLKSLSQEFS
HHHHHHHHHHEEEEHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSMETLLNQLHQYRKEQKVQQKQVKQVSKPSQIVQTKPKLTGFERAEREIIYHMLQSPEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCH
AVRMESHIEDFHTEEHKGILYELYAYYEKGNEPSVGTFLSWLSDEKLKNIITDISTDEFI
HHHHHHHHHHHCCHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
NPEYTEEVLQSHLETLRRHQEKLEKMEIIFKIKQMEKTDPVEAAKYYVAYLQNQKARK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GNRIPEEVVEQIRTSSDIVEVIGEYVQLRKQGRNYFGLCPFHGENSPSFSVSSDKQIFH
CCCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCEEE
CFGCGEGGNVFSFLMKMEGLAFTEAVQKLGERNGIAVAEYTSGQGQQEDISDDTVIMQQA
EEECCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHH
HELLKKYYHHLLVNTEEGNEALSYLLKRGITKEMIEKFEIGYASPAWDAATKILQKRGLS
HHHHHHHHHHHEECCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
LSSMEQAGLLIRSEKDGSHYDRFRGRVMFPIYTLQGKVIAFSGRALGDDTPKYLNSPETP
HHHHHHCCCEEEECCCCCHHHHHCCCEEEEEEEECCEEEEEECCCCCCCCHHHCCCCCCC
IFHKSKLLYNFHQARPFIRKRGQVVLFEGYADVLAAVKSGVEEAVATMGTALTEEQAKLL
CEEHHHHHHHHHHHCHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRNVETVVLCYDGDKAGREATMKAGQLLLQVGCQVKVTSLPDKLDPDEYVQQYGTTAFEN
HCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHCCHHHHH
LVKSSISFVGFKINYLRLGKNLQDESGKEEYVKSVLKELSLLQDAMQAESYLKSLSQEFS
HHHHHHHHHHEEEEHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSMETLLNQLHQYRKEQKVQQKQVKQVSKPSQIVQTKPKLTGFERAEREIIYHMLQSPEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCH
AVRMESHIEDFHTEEHKGILYELYAYYEKGNEPSVGTFLSWLSDEKLKNIITDISTDEFI
HHHHHHHHHHHCCHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
NPEYTEEVLQSHLETLRRHQEKLEKMEIIFKIKQMEKTDPVEAAKYYVAYLQNQKARK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3919021; 3086839; 8969508; 9384377 [H]