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The map label for this gene is ywjE [H]

Identifier: 49184678

GI number: 49184678

Start: 1679058

End: 1680275

Strand: Direct

Name: ywjE [H]

Synonym: BAS1664

Alternate gene names: 49184678

Gene position: 1679058-1680275 (Clockwise)

Preceding gene: 49184677

Following gene: 49184680

Centisome position: 32.11

GC content: 32.1

Gene sequence:

>1218_bases
ATGAACATGATTAAAAAAATATTGCGAGTTACTTCTATTATTATTGCTATTTTCGCTTTTATTTTAATTTGTATGCATAT
TGATGTTACTTTAGGTAGGAAAATGGAAGCTGGGAAAAACATGCCGACAGAGTATGCACCTCATTATAGTGATTTTCAAT
TATATGTAGAAGGAAAGTCATTTTATAAACAGTTATTTACTGATATAAAAGAAGCGAAACAATCTATTTACACTTATTTT
TTTATTTTATCGGATGATAAAAGTAGCCATACTTTTTTAAATTTATTAAAAGAGAAGGCAAAAGAAGGAGTAAACGTATA
TTTATCTGTCGATTTACTTAACGATTTATCATTTGAAAGAAAGATGAAAAAAGAATTACAGGAAAATGGTGTGCATTTTA
CATATAGTAGAAAACAGGAATTACCATTCGGGTTTTATTCCCTTCACCATCGTAATCATCGCCGCATTACAACGATTGAT
GGAGAGATTGGTTATACGGGTGGTTTTAATATAGGGGATGAATACTTAGGGAAAGATAAACACTTTGGGTACTGGAGAGA
CTATCATGTACGGATAAAAGGTGAAGGTGCAAAAGACTTAGAGGAACAATTTGCTTTAGATTGGAAACGAGATACGAAAG
AAGATATAAAGAGAAGTACGGATAAAGCTAGTAAAGGGAATACATTACATACTATGGTTAGTTATAATGGGCATCACGTC
GCTGAAAAATATATAGATCTTATAAAACAAGCTCGGCACTCAATTGTAATTGCAACGCCGTATTTTATAGCGAAAAATAA
AGAATCCATGAATGCTTTAATCGCAGCACAAAAGCGTGGTGTTACAGTAAAAATACTTTGGTCATATAAACCAGATCTAC
CTCTTATAAAAGAAGCGGCGTATCCATACATACGTCAAGCTATTAATAATGGGATTACTGTATATGGGTATAAAAAAGGA
ATGTTCCATGGCAAATTAATGCTTATTGATAATGAATTAACCGTTATTGGCACAACAAACTTTACTTCGCGTAGCTTCAA
TATAAATGATGAAATGAATTTTTATATTCATGGTGGTCCTATTGTAGGGCAAGTTAATAAGGCGTTAACAGAGGATTTTC
GTGACTCAAAAGAAATGACGAAAGAATTTTTTGAGAAGTTATCTTTTTGGGAGCGTTGTAAGGAGAAATTTGCAGGGATG
GTGGATTTTTATTTATAA

Upstream 100 bases:

>100_bases
AAAAGGGTTTGACGTTAAGTCGCATCCTTTTTGTATGAAGCATGAAATCTAAATTTGAATGCCAAAATAGTAGGGTGGAA
AGAAAAATGAGCGGAAGGAA

Downstream 100 bases:

>100_bases
AGGTTGATGGGAAAAGGGTGTCTTTTATAGACACCCTTTTAAAATGAAAAATAATTATTTCTCATGCACAAGTAATGAAG
CGTCATCATTAATCAACGTT

Product: cardiolipin synthetase domain-containing protein

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MNMIKKILRVTSIIIAIFAFILICMHIDVTLGRKMEAGKNMPTEYAPHYSDFQLYVEGKSFYKQLFTDIKEAKQSIYTYF
FILSDDKSSHTFLNLLKEKAKEGVNVYLSVDLLNDLSFERKMKKELQENGVHFTYSRKQELPFGFYSLHHRNHRRITTID
GEIGYTGGFNIGDEYLGKDKHFGYWRDYHVRIKGEGAKDLEEQFALDWKRDTKEDIKRSTDKASKGNTLHTMVSYNGHHV
AEKYIDLIKQARHSIVIATPYFIAKNKESMNALIAAQKRGVTVKILWSYKPDLPLIKEAAYPYIRQAINNGITVYGYKKG
MFHGKLMLIDNELTVIGTTNFTSRSFNINDEMNFYIHGGPIVGQVNKALTEDFRDSKEMTKEFFEKLSFWERCKEKFAGM
VDFYL

Sequences:

>Translated_405_residues
MNMIKKILRVTSIIIAIFAFILICMHIDVTLGRKMEAGKNMPTEYAPHYSDFQLYVEGKSFYKQLFTDIKEAKQSIYTYF
FILSDDKSSHTFLNLLKEKAKEGVNVYLSVDLLNDLSFERKMKKELQENGVHFTYSRKQELPFGFYSLHHRNHRRITTID
GEIGYTGGFNIGDEYLGKDKHFGYWRDYHVRIKGEGAKDLEEQFALDWKRDTKEDIKRSTDKASKGNTLHTMVSYNGHHV
AEKYIDLIKQARHSIVIATPYFIAKNKESMNALIAAQKRGVTVKILWSYKPDLPLIKEAAYPYIRQAINNGITVYGYKKG
MFHGKLMLIDNELTVIGTTNFTSRSFNINDEMNFYIHGGPIVGQVNKALTEDFRDSKEMTKEFFEKLSFWERCKEKFAGM
VDFYL
>Mature_405_residues
MNMIKKILRVTSIIIAIFAFILICMHIDVTLGRKMEAGKNMPTEYAPHYSDFQLYVEGKSFYKQLFTDIKEAKQSIYTYF
FILSDDKSSHTFLNLLKEKAKEGVNVYLSVDLLNDLSFERKMKKELQENGVHFTYSRKQELPFGFYSLHHRNHRRITTID
GEIGYTGGFNIGDEYLGKDKHFGYWRDYHVRIKGEGAKDLEEQFALDWKRDTKEDIKRSTDKASKGNTLHTMVSYNGHHV
AEKYIDLIKQARHSIVIATPYFIAKNKESMNALIAAQKRGVTVKILWSYKPDLPLIKEAAYPYIRQAINNGITVYGYKKG
MFHGKLMLIDNELTVIGTTNFTSRSFNINDEMNFYIHGGPIVGQVNKALTEDFRDSKEMTKEFFEKLSFWERCKEKFAGM
VDFYL

Specific function: Catalyzes The Reversible Phosphatidyl Group Transfer From One Phosphatidylglycerol Molecule To Another To Form Cardiolipin (Cl) (Diphosphatidylglycerol) And Glycerol. Affects Resistance To The Gyrase Inhibitor Novobiocin. [C]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=361, Percent_Identity=25.4847645429363, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1787007, Length=369, Percent_Identity=25.7452574525745, Blast_Score=122, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 47151; Mature: 47151

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMIKKILRVTSIIIAIFAFILICMHIDVTLGRKMEAGKNMPTEYAPHYSDFQLYVEGKS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCEEEEECCHH
FYKQLFTDIKEAKQSIYTYFFILSDDKSSHTFLNLLKEKAKEGVNVYLSVDLLNDLSFER
HHHHHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEEHHCCHHHH
KMKKELQENGVHFTYSRKQELPFGFYSLHHRNHRRITTIDGEIGYTGGFNIGDEYLGKDK
HHHHHHHHCCCEEEECCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHCCCCC
HFGYWRDYHVRIKGEGAKDLEEQFALDWKRDTKEDIKRSTDKASKGNTLHTMVSYNGHHV
CCCCEEEEEEEEECCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCEEEEEEECCCCHH
AEKYIDLIKQARHSIVIATPYFIAKNKESMNALIAAQKRGVTVKILWSYKPDLPLIKEAA
HHHHHHHHHHHHCCEEEECCEEEECCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHC
YPYIRQAINNGITVYGYKKGMFHGKLMLIDNELTVIGTTNFTSRSFNINDEMNFYIHGGP
CHHHHHHHHCCEEEEEEECCCCCCEEEEEECCEEEEECCCCCCCEECCCCCEEEEEECCC
IVGQVNKALTEDFRDSKEMTKEFFEKLSFWERCKEKFAGMVDFYL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCC
>Mature Secondary Structure
MNMIKKILRVTSIIIAIFAFILICMHIDVTLGRKMEAGKNMPTEYAPHYSDFQLYVEGKS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCEEEEECCHH
FYKQLFTDIKEAKQSIYTYFFILSDDKSSHTFLNLLKEKAKEGVNVYLSVDLLNDLSFER
HHHHHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEEHHCCHHHH
KMKKELQENGVHFTYSRKQELPFGFYSLHHRNHRRITTIDGEIGYTGGFNIGDEYLGKDK
HHHHHHHHCCCEEEECCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHCCCCC
HFGYWRDYHVRIKGEGAKDLEEQFALDWKRDTKEDIKRSTDKASKGNTLHTMVSYNGHHV
CCCCEEEEEEEEECCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCEEEEEEECCCCHH
AEKYIDLIKQARHSIVIATPYFIAKNKESMNALIAAQKRGVTVKILWSYKPDLPLIKEAA
HHHHHHHHHHHHCCEEEECCEEEECCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHC
YPYIRQAINNGITVYGYKKGMFHGKLMLIDNELTVIGTTNFTSRSFNINDEMNFYIHGGP
CHHHHHHHHCCEEEEEEECCCCCCEEEEEECCEEEEECCCCCCCEECCCCCEEEEEECCC
IVGQVNKALTEDFRDSKEMTKEFFEKLSFWERCKEKFAGMVDFYL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]