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The map label for this gene is appA [H]

Identifier: 49183251

GI number: 49183251

Start: 219276

End: 220955

Strand: Direct

Name: appA [H]

Synonym: BAS0218

Alternate gene names: 49183251

Gene position: 219276-220955 (Clockwise)

Preceding gene: 49183250

Following gene: 49183252

Centisome position: 4.19

GC content: 34.58

Gene sequence:

>1680_bases
GTGTTATCTGCTTGTGGGGGACAGGAGGATACGGCTTCTACAGAACCAGTAAAAAAGCAAGACTTAAAAGACGCTAAGAT
CGAATCAATTCCAGCTACAGATAAAAAGAAAAGTCCAGAGAAAGCAAATCAGCGAAAGGATACTTTTATTACGGCCATTT
CTAAGCCAGGGGGAGTTTTCCTTCCATATTTCCAAGAGAATGGTTGGGATGGAAATGTAACGTCTGTAATTTTTGCGTCA
CTAGTAACAACAGACAAACAAAGTAAACCTATTCCGGATCTTGCAGAAAAATGGGATATTTCTGCTGACCAGTTAACATA
TACATTCCACTTACGTAAAAATTTAAAATTTAGTGATGGATCGCCTTTAACTGCAGATGACGTAGCATTTACGTTAACAC
TACTTCATGATAAAGCGTATGAAGGTGGATTGGATATTGCTCAGTATGCTGTTAAAGGCGGGAAAGAATATAAAGAAGGA
AAAACGACTTCTATTGAAGGGATTCAAGTTGTTGATCCACAGACAATTAAAATTACAACTGAAAAAGTTAATTCACAGGC
GCTAACTAATTTAGGTGGCCCAGTGCTATCAAAAGCTTATTATGGAAAAGATTATAAACAAAATACAAGTTTGGACTATT
TAAAAGCATTATATGGGCAACCAATTGCAGCGGGTCCATATAAATTTGAAAAATATGTTCCAGGTCAAGAAGTACGCTTT
GTTTCTAACGAACATTATTATGCAGGTAAACCAAAAATCAAAAACTTTATTTATAAAATCACATCAGGTGATACTGGATT
CCAATTATTCCAAACAGGTGAACTGGACTATAGTGGATTTAGAGCGAACCCTGAAAATATAGACCAATTAAAAGGATTAG
AGTTTGCAAATATTAATCTTGAATCATCAAGTGATATTGCTTATATCTATGTAAATAATAAGAAGTCGTATTTGAAAGAT
AAAAAGGTACGCCAAGCACTTACTTATGGATTAGACCGTAAGAAGTATGTGGATACAGCTTTACAAGGATATGGCTCTGT
TGCTAATGTACCAATTGCTCCAGTTTCATGGGCATATACAGAAGAAGGTATTAATAAATATCCACACGACGTAGAAAAGG
CTAAAAAATTATTGGATGAAGCTGGTTGGAAAGTAGGTTCTGACGGGGTTCGTGAAAAAGATGGTCAAAAGTTAAAATTA
ACATACTATGCATCAAATACAGGTAAGACAAATGATATCTTTATTCCAATTGCAAAAGAAAGTTACAAAGAAATTGGTGT
TGAATTAAATCCAGAGTTGATGGACTTTAATACGATGCTTTCAAAAGTAGGAAAAGGTGACTACGATTTAGCGGCAGTTT
CAACACCAGGGATTAGTGATCCAAGTGAAGTTGTAAGTGAGTACTTATCAACTAATCCAAAAAGTGATACGGGTTATAAT
AATCCGAAAGTAGATGAGTTGATTGTAAAAGGTATAGGAACGACAGATATTGAAAAACGTAAAGCGATTTATAAAGAGCT
GTATAAAGAGCTGTATAAAGAGTTAAGTGATGATCCACCAGTTATTTTATTAAACTATCGTAAACTACTTTATGCTCATA
ATGCACGTATAAAAGGAATTGATCCAGAAAAGTACGATAGCATCAGTTCCAACTTACCAGTGTTATCTATTGAACAATAA

Upstream 100 bases:

>100_bases
AGTTCAAAGGGTATCACTATTAAAAAGTTAGGGTGGGGACAATGAAGAAAAAGACAAAAAATGGGCTGGTGTATTTTCGG
TATTACTGGGTAGTTCGCTT

Downstream 100 bases:

>100_bases
GGGTTGACCTAAATAATAGAAGACGTTTTTTCACACAGCAATGAAATGGTAGCTGTTTTGAAAAAATGTTCTTCTATTTT
TAAGTAAATAGGATTGGGGA

Product: oligopeptide ABC transporter oligopeptide-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 559; Mature: 559

Protein sequence:

>559_residues
MLSACGGQEDTASTEPVKKQDLKDAKIESIPATDKKKSPEKANQRKDTFITAISKPGGVFLPYFQENGWDGNVTSVIFAS
LVTTDKQSKPIPDLAEKWDISADQLTYTFHLRKNLKFSDGSPLTADDVAFTLTLLHDKAYEGGLDIAQYAVKGGKEYKEG
KTTSIEGIQVVDPQTIKITTEKVNSQALTNLGGPVLSKAYYGKDYKQNTSLDYLKALYGQPIAAGPYKFEKYVPGQEVRF
VSNEHYYAGKPKIKNFIYKITSGDTGFQLFQTGELDYSGFRANPENIDQLKGLEFANINLESSSDIAYIYVNNKKSYLKD
KKVRQALTYGLDRKKYVDTALQGYGSVANVPIAPVSWAYTEEGINKYPHDVEKAKKLLDEAGWKVGSDGVREKDGQKLKL
TYYASNTGKTNDIFIPIAKESYKEIGVELNPELMDFNTMLSKVGKGDYDLAAVSTPGISDPSEVVSEYLSTNPKSDTGYN
NPKVDELIVKGIGTTDIEKRKAIYKELYKELYKELSDDPPVILLNYRKLLYAHNARIKGIDPEKYDSISSNLPVLSIEQ

Sequences:

>Translated_559_residues
MLSACGGQEDTASTEPVKKQDLKDAKIESIPATDKKKSPEKANQRKDTFITAISKPGGVFLPYFQENGWDGNVTSVIFAS
LVTTDKQSKPIPDLAEKWDISADQLTYTFHLRKNLKFSDGSPLTADDVAFTLTLLHDKAYEGGLDIAQYAVKGGKEYKEG
KTTSIEGIQVVDPQTIKITTEKVNSQALTNLGGPVLSKAYYGKDYKQNTSLDYLKALYGQPIAAGPYKFEKYVPGQEVRF
VSNEHYYAGKPKIKNFIYKITSGDTGFQLFQTGELDYSGFRANPENIDQLKGLEFANINLESSSDIAYIYVNNKKSYLKD
KKVRQALTYGLDRKKYVDTALQGYGSVANVPIAPVSWAYTEEGINKYPHDVEKAKKLLDEAGWKVGSDGVREKDGQKLKL
TYYASNTGKTNDIFIPIAKESYKEIGVELNPELMDFNTMLSKVGKGDYDLAAVSTPGISDPSEVVSEYLSTNPKSDTGYN
NPKVDELIVKGIGTTDIEKRKAIYKELYKELYKELSDDPPVILLNYRKLLYAHNARIKGIDPEKYDSISSNLPVLSIEQ
>Mature_559_residues
MLSACGGQEDTASTEPVKKQDLKDAKIESIPATDKKKSPEKANQRKDTFITAISKPGGVFLPYFQENGWDGNVTSVIFAS
LVTTDKQSKPIPDLAEKWDISADQLTYTFHLRKNLKFSDGSPLTADDVAFTLTLLHDKAYEGGLDIAQYAVKGGKEYKEG
KTTSIEGIQVVDPQTIKITTEKVNSQALTNLGGPVLSKAYYGKDYKQNTSLDYLKALYGQPIAAGPYKFEKYVPGQEVRF
VSNEHYYAGKPKIKNFIYKITSGDTGFQLFQTGELDYSGFRANPENIDQLKGLEFANINLESSSDIAYIYVNNKKSYLKD
KKVRQALTYGLDRKKYVDTALQGYGSVANVPIAPVSWAYTEEGINKYPHDVEKAKKLLDEAGWKVGSDGVREKDGQKLKL
TYYASNTGKTNDIFIPIAKESYKEIGVELNPELMDFNTMLSKVGKGDYDLAAVSTPGISDPSEVVSEYLSTNPKSDTGYN
NPKVDELIVKGIGTTDIEKRKAIYKELYKELYKELSDDPPVILLNYRKLLYAHNARIKGIDPEKYDSISSNLPVLSIEQ

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=474, Percent_Identity=27.4261603375527, Blast_Score=135, Evalue=8e-33,
Organism=Escherichia coli, GI1787762, Length=459, Percent_Identity=27.0152505446623, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1789887, Length=462, Percent_Identity=24.8917748917749, Blast_Score=125, Evalue=1e-29,
Organism=Escherichia coli, GI1787052, Length=501, Percent_Identity=26.1477045908184, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI1787495, Length=485, Percent_Identity=24.1237113402062, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787551, Length=340, Percent_Identity=24.1176470588235, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI87081878, Length=477, Percent_Identity=24.1090146750524, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1789397, Length=444, Percent_Identity=22.2972972972973, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 62195; Mature: 62195

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSACGGQEDTASTEPVKKQDLKDAKIESIPATDKKKSPEKANQRKDTFITAISKPGGVF
CCCCCCCCCCCCCCCCCHHHCCCCCHHCCCCCCCCCCCCHHHHCCCCEEEEEECCCCCEE
LPYFQENGWDGNVTSVIFASLVTTDKQSKPIPDLAEKWDISADQLTYTFHLRKNLKFSDG
EEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHCCCCHHHEEEEEEEECCCCCCCC
SPLTADDVAFTLTLLHDKAYEGGLDIAQYAVKGGKEYKEGKTTSIEGIQVVDPQTIKITT
CCCCHHHHEEEEEEHHHHCCCCCCHHHHHHHHCCCHHCCCCCCCCCCEEEECCCEEEEEH
EKVNSQALTNLGGPVLSKAYYGKDYKQNTSLDYLKALYGQPIAAGPYKFEKYVPGQEVRF
HHCCHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHCCCCCCEEE
VSNEHYYAGKPKIKNFIYKITSGDTGFQLFQTGELDYSGFRANPENIDQLKGLEFANINL
ECCCCEECCCCHHCEEEEEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHCCCEEEEEEE
ESSSDIAYIYVNNKKSYLKDKKVRQALTYGLDRKKYVDTALQGYGSVANVPIAPVSWAYT
CCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCEEHHH
EEGINKYPHDVEKAKKLLDEAGWKVGSDGVREKDGQKLKLTYYASNTGKTNDIFIPIAKE
HHHHHCCCHHHHHHHHHHHHCCCEECCCCCCCCCCCEEEEEEEECCCCCCCEEEEEECHH
SYKEIGVELNPELMDFNTMLSKVGKGDYDLAAVSTPGISDPSEVVSEYLSTNPKSDTGYN
HHHHCCCEECCHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCCCC
NPKVDELIVKGIGTTDIEKRKAIYKELYKELYKELSDDPPVILLNYRKLLYAHNARIKGI
CCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHCCCEEECC
DPEKYDSISSNLPVLSIEQ
CCHHHHHHHCCCCEEEECC
>Mature Secondary Structure
MLSACGGQEDTASTEPVKKQDLKDAKIESIPATDKKKSPEKANQRKDTFITAISKPGGVF
CCCCCCCCCCCCCCCCCHHHCCCCCHHCCCCCCCCCCCCHHHHCCCCEEEEEECCCCCEE
LPYFQENGWDGNVTSVIFASLVTTDKQSKPIPDLAEKWDISADQLTYTFHLRKNLKFSDG
EEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHCCCCHHHEEEEEEEECCCCCCCC
SPLTADDVAFTLTLLHDKAYEGGLDIAQYAVKGGKEYKEGKTTSIEGIQVVDPQTIKITT
CCCCHHHHEEEEEEHHHHCCCCCCHHHHHHHHCCCHHCCCCCCCCCCEEEECCCEEEEEH
EKVNSQALTNLGGPVLSKAYYGKDYKQNTSLDYLKALYGQPIAAGPYKFEKYVPGQEVRF
HHCCHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHCCCCCCEEE
VSNEHYYAGKPKIKNFIYKITSGDTGFQLFQTGELDYSGFRANPENIDQLKGLEFANINL
ECCCCEECCCCHHCEEEEEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHCCCEEEEEEE
ESSSDIAYIYVNNKKSYLKDKKVRQALTYGLDRKKYVDTALQGYGSVANVPIAPVSWAYT
CCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCEEHHH
EEGINKYPHDVEKAKKLLDEAGWKVGSDGVREKDGQKLKLTYYASNTGKTNDIFIPIAKE
HHHHHCCCHHHHHHHHHHHHCCCEECCCCCCCCCCCEEEEEEEECCCCCCCEEEEEECHH
SYKEIGVELNPELMDFNTMLSKVGKGDYDLAAVSTPGISDPSEVVSEYLSTNPKSDTGYN
HHHHCCCEECCHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCCCC
NPKVDELIVKGIGTTDIEKRKAIYKELYKELYKELSDDPPVILLNYRKLLYAHNARIKGI
CCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHCCCEEECC
DPEKYDSISSNLPVLSIEQ
CCHHHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]