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The map label for this gene is gspF [H]

Identifier: 46447530

GI number: 46447530

Start: 2256916

End: 2258103

Strand: Reverse

Name: gspF [H]

Synonym: pc1896

Alternate gene names: 46447530

Gene position: 2258103-2256916 (Counterclockwise)

Preceding gene: 46447531

Following gene: 46447529

Centisome position: 93.52

GC content: 38.72

Gene sequence:

>1188_bases
ATGCCGCTTTATCAATATCAGTATGTCGATGAGAAAAAAAAACGTCGAACAGGAGTGATTGAAGCGATCAGTGAACGTGA
TGCTAAAGAAAAATTGCGCGATCAAGGATTACTCATTACGCAGATTCAAACAAAAAGTAAAATTAACGGAAAGCAAAACC
TAAAAGGAGAGGCTCTCTTAGCTTTTACAGTTCAACTTTCTCAATTAGTCAATGCTGGTATTCCCCTTTATGAAAGCTTA
ATTGCTATTGAAGAGCAATCGAGAGGAGAGTCTTTTCACCGCGTTGTTTTAAGTTTATGTGAACAAATTCGTACAGGAAT
GAGTTTGTCTGGAGCAATGGCAACGTATTCGGAGAGTTTTGACCAACTGTATTGCGGAATGGTAGCGGCTGGAGAAGCTG
TTGGGATGCTAGGTCCGATTTTAGAAAAACTCACGCAACTTCTTGCCAAACAAATGAAATTAAAAAAACAAATCACGACA
GCAATGATTTATCCTTGTATCTTAGGGGGATTTTCGTTGTTGATTATCACCCTCTTACTTGGATTTGTCGTTCCCTCTTT
AGAAGGAATTTTTGCAGAACGTAAACTCAATGCTTTTACAAATGCCATTTTAGGTTTAAGCCACTTTTTTAGAAATTATT
GGTGGTTATACTTGCCTGCTTTCGTAGCAGGAACAGTTTTTGTCATTTGGAAATTTCGTTCTTCAGAAGGAAAGTTATGG
ATGGAAAAAACGCTTTTGCGCATACCTGTGATTAATACTTTAGTTATTCAAACGGCTGTGGCAAGATTTTGCCGTACGAT
GGGAACGCTTCTTCAGGGGGGATTGAACATGATAGAGTCTCTTCGCATCTCTCGTGGAGTGATGCATAATGTGGTTTTGG
AAAAAGAAATTCAACAGGCTGAAGCGAAGATTATCGAAGGGCACTCTTTAAGCCAAGAATTAGGGAAGTCTAAATATATT
CCTCCATTGGTTTCAAAAATGTTAGCTGTTGGTGAAGAATCAGGGACGTCTATCGTGATGTTAAGTCGTATTGCAGATAT
GTATGAACAAGAAATTGAGAAAACATTAGATCGGGTGATGGCTTTGGCACAACCAGTTATTTTAATTGTGATGGGACTCG
TTATTGGCACAGTGCTTTTGGCTATTATGTTGCCATTAACAGATGTCAGCTCTTTTTCAGCAGGTTGA

Upstream 100 bases:

>100_bases
TGAGCTTACTTGGTCATGGAGCAGAACTGGTTAAACAAGGTGTTTCGACTGTAGCAGAAGTACTGCGCGTGGCAAGAGGA
ATTGAGGAAGAAGGGTAGGT

Downstream 100 bases:

>100_bases
AAAAATAAAGAGGATGTTATGAAAGTTGTAAAAAGTAAAAAGCATTTTGTTACACTTGTAGAAATGATGATCGTCATGTT
CTTAATCGCAATGATTACAG

Product: putative protein of the general secretion pathway

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MPLYQYQYVDEKKKRRTGVIEAISERDAKEKLRDQGLLITQIQTKSKINGKQNLKGEALLAFTVQLSQLVNAGIPLYESL
IAIEEQSRGESFHRVVLSLCEQIRTGMSLSGAMATYSESFDQLYCGMVAAGEAVGMLGPILEKLTQLLAKQMKLKKQITT
AMIYPCILGGFSLLIITLLLGFVVPSLEGIFAERKLNAFTNAILGLSHFFRNYWWLYLPAFVAGTVFVIWKFRSSEGKLW
MEKTLLRIPVINTLVIQTAVARFCRTMGTLLQGGLNMIESLRISRGVMHNVVLEKEIQQAEAKIIEGHSLSQELGKSKYI
PPLVSKMLAVGEESGTSIVMLSRIADMYEQEIEKTLDRVMALAQPVILIVMGLVIGTVLLAIMLPLTDVSSFSAG

Sequences:

>Translated_395_residues
MPLYQYQYVDEKKKRRTGVIEAISERDAKEKLRDQGLLITQIQTKSKINGKQNLKGEALLAFTVQLSQLVNAGIPLYESL
IAIEEQSRGESFHRVVLSLCEQIRTGMSLSGAMATYSESFDQLYCGMVAAGEAVGMLGPILEKLTQLLAKQMKLKKQITT
AMIYPCILGGFSLLIITLLLGFVVPSLEGIFAERKLNAFTNAILGLSHFFRNYWWLYLPAFVAGTVFVIWKFRSSEGKLW
MEKTLLRIPVINTLVIQTAVARFCRTMGTLLQGGLNMIESLRISRGVMHNVVLEKEIQQAEAKIIEGHSLSQELGKSKYI
PPLVSKMLAVGEESGTSIVMLSRIADMYEQEIEKTLDRVMALAQPVILIVMGLVIGTVLLAIMLPLTDVSSFSAG
>Mature_394_residues
PLYQYQYVDEKKKRRTGVIEAISERDAKEKLRDQGLLITQIQTKSKINGKQNLKGEALLAFTVQLSQLVNAGIPLYESLI
AIEEQSRGESFHRVVLSLCEQIRTGMSLSGAMATYSESFDQLYCGMVAAGEAVGMLGPILEKLTQLLAKQMKLKKQITTA
MIYPCILGGFSLLIITLLLGFVVPSLEGIFAERKLNAFTNAILGLSHFFRNYWWLYLPAFVAGTVFVIWKFRSSEGKLWM
EKTLLRIPVINTLVIQTAVARFCRTMGTLLQGGLNMIESLRISRGVMHNVVLEKEIQQAEAKIIEGHSLSQELGKSKYIP
PLVSKMLAVGEESGTSIVMLSRIADMYEQEIEKTLDRVMALAQPVILIVMGLVIGTVLLAIMLPLTDVSSFSAG

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=395, Percent_Identity=28.1012658227848, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1786295, Length=399, Percent_Identity=24.3107769423559, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 43859; Mature: 43728

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: PS00874 T2SP_F

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLYQYQYVDEKKKRRTGVIEAISERDAKEKLRDQGLLITQIQTKSKINGKQNLKGEALL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHCCCCCCCCCCHHH
AFTVQLSQLVNAGIPLYESLIAIEEQSRGESFHRVVLSLCEQIRTGMSLSGAMATYSESF
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
DQLYCGMVAAGEAVGMLGPILEKLTQLLAKQMKLKKQITTAMIYPCILGGFSLLIITLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFVVPSLEGIFAERKLNAFTNAILGLSHFFRNYWWLYLPAFVAGTVFVIWKFRSSEGKLW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCH
MEKTLLRIPVINTLVIQTAVARFCRTMGTLLQGGLNMIESLRISRGVMHNVVLEKEIQQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAKIIEGHSLSQELGKSKYIPPLVSKMLAVGEESGTSIVMLSRIADMYEQEIEKTLDRVM
HHHHHCCCCHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ALAQPVILIVMGLVIGTVLLAIMLPLTDVSSFSAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PLYQYQYVDEKKKRRTGVIEAISERDAKEKLRDQGLLITQIQTKSKINGKQNLKGEALL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHCCCCCCCCCCHHH
AFTVQLSQLVNAGIPLYESLIAIEEQSRGESFHRVVLSLCEQIRTGMSLSGAMATYSESF
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
DQLYCGMVAAGEAVGMLGPILEKLTQLLAKQMKLKKQITTAMIYPCILGGFSLLIITLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFVVPSLEGIFAERKLNAFTNAILGLSHFFRNYWWLYLPAFVAGTVFVIWKFRSSEGKLW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCH
MEKTLLRIPVINTLVIQTAVARFCRTMGTLLQGGLNMIESLRISRGVMHNVVLEKEIQQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAKIIEGHSLSQELGKSKYIPPLVSKMLAVGEESGTSIVMLSRIADMYEQEIEKTLDRVM
HHHHHCCCCHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ALAQPVILIVMGLVIGTVLLAIMLPLTDVSSFSAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1944223; 12024217 [H]