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The map label for this gene is gspE [H]

Identifier: 46447531

GI number: 46447531

Start: 2258106

End: 2259737

Strand: Reverse

Name: gspE [H]

Synonym: pc1897

Alternate gene names: 46447531

Gene position: 2259737-2258106 (Counterclockwise)

Preceding gene: 46447532

Following gene: 46447530

Centisome position: 93.59

GC content: 40.2

Gene sequence:

>1632_bases
ATGACGGGCGATAATGAGGAAACAGGAAATAAAGAGTTTTTATTAAAAGAAAAATTAGGAGGAGAACAAGCTCAAGCGAA
GCTTGCCGAACAATTTGGGATGACTTGTTACCTTGACTTGTCTTCTTTTCGAGTAAGTAAAGAGCGTTATAGACAAGTTC
CTTATGCATTTGCCAAAAAGCATATTATTCTTCCTGTGGAAGATCAGGGAACTATCGTAGTTGTCGCTGTGGCTGATCCT
CTTAATTTAGCTCCACTAGAAGAGCTTCGGTTTTTTTTAAACGCTGAAATTGAAGCCGTTTATAGCCCTCGTGATGTGAT
TTTGTCAGCTATACATGATTGTTATAACACTGAACATGGTGCAGCTTCGCAACTGATTGCTGGGATGGGAGACAAAAGCG
AAGAAGGAAAAGAAGGCGATATTGAAATTTTTGATCTTTTTGATCAAAGCAAGCTGCAATCCCCAGTTGTCCAGCTCCTT
AATTTAATTTTAACAGAAGCCATTCAGCAAGGGGCTTCTGATATTCATTTTGAGCCTTCAGAAAATGGAATGCGGGTTCG
ATATCGCATTGATGGAGTTTTGCAAAATAGGCATGCTCCTTCGCAAGATTACCAAACTCAATTATTGACTCGTATCAAAG
TCATGTCCAAATTAGATATTGCCGAGCATCGTTTGCCGCAAGATGGACGTATCAAATTACGTATGGGAAGACGGGAAGTA
GATTTTCGTGTAAGTACAGTGCCAATTGCAGGAGGCGAGCGTATTGTTTTGCGTATTTTAGATAAAGGAAATGTCTTACT
TGGATTGGATAAAATTGGGATGCTTCCTTCTGTTTTTGAGCAATTTCAAAAGCTCGTTTCATTACCAGAAGGTATTGTTT
TAGTGACGGGGCCAACGGGAAGCGGAAAAACGACAACTCTTTATAGTGCTATTTGTGATATGACAAATGATGAGATCAAC
ATCATGACCATTGAAGATCCAGTAGAGTATAATTTAAGAGGGATTGCACAAATTGGAGTCCACCATAAAATTAAGCTCGA
TTTTGCTACGGGTTTAAGACATATCTTACGTCAAGACCCCGATGTGATTATGGTGGGAGAAATTAGAGATAAAGAAACAG
CAGAAATAGCCATTCAAGCTGCTTTGACAGGGCACCTCGTTTTAAGTACGCTTCATACCAATGATGCTCCTTCTGCGATT
ACTCGCCTTGTCGATATGGGAATTGAACCTTATTTATTGTCTTCTTGTATTGTAGGTGTTCTAGCTCAAAGGCTTGTCAG
AAGAATATGTTCAGATTGTAAAGAAACTTATGAACCGAGTCCTCGAGAGTTGCAAAGTTTGGGAATTCGGTCAGAAGAAT
TGAAAAATGGTTTCCTCTATCGAGGTAAAGGGTGTTCCTCTTGTTATGGAACTGGGTTTAAAGGGCGTCAGGGCGTTTAT
GAGTTAATGCCAGTTAATCATGCAATCAATAAACAAATTGTCCAAAGTCCAGATGCCATAGAAATGCGTAAAGTGGCTTT
GTCACAAGGAATGGTGAGCTTACTTGGTCATGGAGCAGAACTGGTTAAACAAGGTGTTTCGACTGTAGCAGAAGTACTGC
GCGTGGCAAGAGGAATTGAGGAAGAAGGGTAG

Upstream 100 bases:

>100_bases
ATGGAAGGGAGTATGACGGTATTATTCGGTCGTCCTCGTGAACGTTATTACATCTCTGATTGCTGCAAAGTAGATGACCA
TAGCCAACTTGAAGGAGAAT

Downstream 100 bases:

>100_bases
GTATGCCGCTTTATCAATATCAGTATGTCGATGAGAAAAAAAAACGTCGAACAGGAGTGATTGAAGCGATCAGTGAACGT
GATGCTAAAGAAAAATTGCG

Product: general secretion pathway protein

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 543; Mature: 542

Protein sequence:

>543_residues
MTGDNEETGNKEFLLKEKLGGEQAQAKLAEQFGMTCYLDLSSFRVSKERYRQVPYAFAKKHIILPVEDQGTIVVVAVADP
LNLAPLEELRFFLNAEIEAVYSPRDVILSAIHDCYNTEHGAASQLIAGMGDKSEEGKEGDIEIFDLFDQSKLQSPVVQLL
NLILTEAIQQGASDIHFEPSENGMRVRYRIDGVLQNRHAPSQDYQTQLLTRIKVMSKLDIAEHRLPQDGRIKLRMGRREV
DFRVSTVPIAGGERIVLRILDKGNVLLGLDKIGMLPSVFEQFQKLVSLPEGIVLVTGPTGSGKTTTLYSAICDMTNDEIN
IMTIEDPVEYNLRGIAQIGVHHKIKLDFATGLRHILRQDPDVIMVGEIRDKETAEIAIQAALTGHLVLSTLHTNDAPSAI
TRLVDMGIEPYLLSSCIVGVLAQRLVRRICSDCKETYEPSPRELQSLGIRSEELKNGFLYRGKGCSSCYGTGFKGRQGVY
ELMPVNHAINKQIVQSPDAIEMRKVALSQGMVSLLGHGAELVKQGVSTVAEVLRVARGIEEEG

Sequences:

>Translated_543_residues
MTGDNEETGNKEFLLKEKLGGEQAQAKLAEQFGMTCYLDLSSFRVSKERYRQVPYAFAKKHIILPVEDQGTIVVVAVADP
LNLAPLEELRFFLNAEIEAVYSPRDVILSAIHDCYNTEHGAASQLIAGMGDKSEEGKEGDIEIFDLFDQSKLQSPVVQLL
NLILTEAIQQGASDIHFEPSENGMRVRYRIDGVLQNRHAPSQDYQTQLLTRIKVMSKLDIAEHRLPQDGRIKLRMGRREV
DFRVSTVPIAGGERIVLRILDKGNVLLGLDKIGMLPSVFEQFQKLVSLPEGIVLVTGPTGSGKTTTLYSAICDMTNDEIN
IMTIEDPVEYNLRGIAQIGVHHKIKLDFATGLRHILRQDPDVIMVGEIRDKETAEIAIQAALTGHLVLSTLHTNDAPSAI
TRLVDMGIEPYLLSSCIVGVLAQRLVRRICSDCKETYEPSPRELQSLGIRSEELKNGFLYRGKGCSSCYGTGFKGRQGVY
ELMPVNHAINKQIVQSPDAIEMRKVALSQGMVSLLGHGAELVKQGVSTVAEVLRVARGIEEEG
>Mature_542_residues
TGDNEETGNKEFLLKEKLGGEQAQAKLAEQFGMTCYLDLSSFRVSKERYRQVPYAFAKKHIILPVEDQGTIVVVAVADPL
NLAPLEELRFFLNAEIEAVYSPRDVILSAIHDCYNTEHGAASQLIAGMGDKSEEGKEGDIEIFDLFDQSKLQSPVVQLLN
LILTEAIQQGASDIHFEPSENGMRVRYRIDGVLQNRHAPSQDYQTQLLTRIKVMSKLDIAEHRLPQDGRIKLRMGRREVD
FRVSTVPIAGGERIVLRILDKGNVLLGLDKIGMLPSVFEQFQKLVSLPEGIVLVTGPTGSGKTTTLYSAICDMTNDEINI
MTIEDPVEYNLRGIAQIGVHHKIKLDFATGLRHILRQDPDVIMVGEIRDKETAEIAIQAALTGHLVLSTLHTNDAPSAIT
RLVDMGIEPYLLSSCIVGVLAQRLVRRICSDCKETYEPSPRELQSLGIRSEELKNGFLYRGKGCSSCYGTGFKGRQGVYE
LMPVNHAINKQIVQSPDAIEMRKVALSQGMVSLLGHGAELVKQGVSTVAEVLRVARGIEEEG

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=397, Percent_Identity=48.1108312342569, Blast_Score=377, Evalue=1e-105,
Organism=Escherichia coli, GI1786296, Length=381, Percent_Identity=44.3569553805774, Blast_Score=318, Evalue=7e-88,
Organism=Escherichia coli, GI87082188, Length=148, Percent_Identity=47.972972972973, Blast_Score=116, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 60061; Mature: 59930

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGDNEETGNKEFLLKEKLGGEQAQAKLAEQFGMTCYLDLSSFRVSKERYRQVPYAFAKK
CCCCCCCCCCHHEEEHHHCCCHHHHHHHHHHHCCEEEEEHHHHHHCHHHHHHCCHHHHCC
HIILPVEDQGTIVVVAVADPLNLAPLEELRFFLNAEIEAVYSPRDVILSAIHDCYNTEHG
EEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHCCCCEEEECHHHHHHHHHHHHHCCCCC
AASQLIAGMGDKSEEGKEGDIEIFDLFDQSKLQSPVVQLLNLILTEAIQQGASDIHFEPS
HHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCC
ENGMRVRYRIDGVLQNRHAPSQDYQTQLLTRIKVMSKLDIAEHRLPQDGRIKLRMGRREV
CCCCEEEEEECHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCEEE
DFRVSTVPIAGGERIVLRILDKGNVLLGLDKIGMLPSVFEQFQKLVSLPEGIVLVTGPTG
CEEEEEEEECCCCEEEEEEECCCCEEEECHHHCCCHHHHHHHHHHHCCCCCEEEEECCCC
SGKTTTLYSAICDMTNDEINIMTIEDPVEYNLRGIAQIGVHHKIKLDFATGLRHILRQDP
CCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHEEECCCEEEEEEHHHHHHHHHHCCC
DVIMVGEIRDKETAEIAIQAALTGHLVLSTLHTNDAPSAITRLVDMGIEPYLLSSCIVGV
CEEEEECCCCCCHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHH
LAQRLVRRICSDCKETYEPSPRELQSLGIRSEELKNGFLYRGKGCSSCYGTGFKGRQGVY
HHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHCCCCEEECCCCCCCCCCCCCCCCCCC
ELMPVNHAINKQIVQSPDAIEMRKVALSQGMVSLLGHGAELVKQGVSTVAEVLRVARGIE
EEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC
EEG
CCC
>Mature Secondary Structure 
TGDNEETGNKEFLLKEKLGGEQAQAKLAEQFGMTCYLDLSSFRVSKERYRQVPYAFAKK
CCCCCCCCCHHEEEHHHCCCHHHHHHHHHHHCCEEEEEHHHHHHCHHHHHHCCHHHHCC
HIILPVEDQGTIVVVAVADPLNLAPLEELRFFLNAEIEAVYSPRDVILSAIHDCYNTEHG
EEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHCCCCEEEECHHHHHHHHHHHHHCCCCC
AASQLIAGMGDKSEEGKEGDIEIFDLFDQSKLQSPVVQLLNLILTEAIQQGASDIHFEPS
HHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCC
ENGMRVRYRIDGVLQNRHAPSQDYQTQLLTRIKVMSKLDIAEHRLPQDGRIKLRMGRREV
CCCCEEEEEECHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCEEE
DFRVSTVPIAGGERIVLRILDKGNVLLGLDKIGMLPSVFEQFQKLVSLPEGIVLVTGPTG
CEEEEEEEECCCCEEEEEEECCCCEEEECHHHCCCHHHHHHHHHHHCCCCCEEEEECCCC
SGKTTTLYSAICDMTNDEINIMTIEDPVEYNLRGIAQIGVHHKIKLDFATGLRHILRQDP
CCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHEEECCCEEEEEEHHHHHHHHHHCCC
DVIMVGEIRDKETAEIAIQAALTGHLVLSTLHTNDAPSAITRLVDMGIEPYLLSSCIVGV
CEEEEECCCCCCHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHH
LAQRLVRRICSDCKETYEPSPRELQSLGIRSEELKNGFLYRGKGCSSCYGTGFKGRQGVY
HHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHCCCCEEECCCCCCCCCCCCCCCCCCC
ELMPVNHAINKQIVQSPDAIEMRKVALSQGMVSLLGHGAELVKQGVSTVAEVLRVARGIE
EEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC
EEG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]