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The map label for this gene is dnaG [H]

Identifier: 46446324

GI number: 46446324

Start: 839989

End: 841767

Strand: Direct

Name: dnaG [H]

Synonym: pc0690

Alternate gene names: 46446324

Gene position: 839989-841767 (Clockwise)

Preceding gene: 46446315

Following gene: 46446325

Centisome position: 34.79

GC content: 36.48

Gene sequence:

>1779_bases
ATGCCTATTTTTAATAAAGAAAGTTTAGAAAATCTGAGACAACGAGTCGATTTAGTCGAAGTATTATCTTCTCATATCGA
ACTTAAGCGCAGTGGAGCTTCTTATAAAGGTCTCTGTCCGTTTCATGACGAAAAATCTCCTTCTTTTATCGTTCAAAAAG
GAGATTCTCATTATCATTGTTTCGGTTGCGGGGCACATGGAGATGCAATTCAATTTTTGATGTCCCATCAAAAGCTCAGT
TTTGCCGAATCAGTTGAAAGTTTAGCCCAACGCTTTCAGGTTCATTTAGAACTAGTGGAAGATCGAGAAGAAAAAAAAGG
GGTGACCAAAGCTTTTTTAAAGTTAGCATTAGAGACTGCTTCTCAGTTTTTTCATTATTGTCTGCTCTATTCAGAAGAAG
GGCATGAAGCATTAAATTATCTTTATAATCGAGGCATTGATTTAGATTTTATTTGCCATTTTCAAGTGGGGTTGGCTCCG
AAAACGGCAGGGATTTTTCGTAAATTTATGCATGCAAAAGGAATCAAAGATGACTCGTTATTAGAAGCTGGTTTGTTGAG
TGTGAATAAAGATGGTCAAGTCAGAGAGTTTTTTAATGATCGCATTCTTTTTCCCATTCACCACCATTCTCAAGGAGTGA
TTGGTTTTTCAGGAAGGAAATATAAGGAAGAAACATTTGGAGGAAAGTATATCAATACTCCAGAAACTTCCCTATTTAAA
AAATCTCGGGTTTTATTTGGATTGAATTATTCTCGTCGCAGAATAGCAAAAGAGCGCAAAGCCATTATTGTAGAAGGACA
AATTGATGCCCTTCGTCTCATTCAAATGGGATTTAATTTAACAGTGGCAGGGCAAGGAACAGCATTTGGTGAAGGGCATG
TTCAAGAACTGATCAATTTAGGTGTGAATCAAGTTTTTTTAGCTCTCGACTCTGACCTTGCTGGACAAGAAGCAACAAGC
AAAATTGGCCATCTTTTTCAAAAAGAGGGGATTGAAGTTCGCATTGTGCAATTGCCAGTTGGGGGAGATCCCGATAGTTT
TTTGAGAGAGCAAGGACCTGAAGCCTTTTTAGAATTGCTCAAAAATAGCTCTGATTATTTAAACTTTTTAATTAAACATC
TCTCTCAAGACTTAAATTTAGATTCTCCTGCCGCTAAAAATGAGCTAGTTCAAAAAGCAACTAAGCTTATTCGTGAATGG
GATCATCCTCTCATGGTCCATGAAACTCTTCGAAAATTAGCCCATCTCATGAAAGTACCCGAAGAAATTATCGGTGTGGG
CAAAAACCATTTACCCAATATTTATATTAAAAAATCTGCAAGCGTTGGGGCACAAACGATTGACCCTGACCGAATTCTAG
AAACGGATCTTTTAAGATGGCTTTTATTACTGGGTCAAGAACAGACAAAGCTGGTTGAAATAGTCAGAACAAATTTAGTA
AAAGAAGATTTTCGCGTTGCCATTTGTCAAAAAATTTATGATATCTATCGTAATAATTATGAGAATCAACGCTCCTGTGA
TTTATTATCGCTAGCGATTGACTTAGATGATGCAGAGGGACAGTTGGTTTTATCAGACCTATTGCAAAAAAAAGTGAACA
AAGAAAAAGCTGAGCAATTGCTAATTGAAACAGTCAAAAAAATATTAGATCGAAATTGGATGCACAAAAGAGAAGAAATT
AAAATTAAAGTGCAAAGTGGTCATTGCTCAGACGATGAGGTGATGGAGTTGATTAAACAGTTTGATGAGTTAAAACGAAA
TCCCCCTATTGTCAAATGA

Upstream 100 bases:

>100_bases
CTAATTATGTTTGTTAAAAAAAATAGATTTCTCAAACCTTCTTCGATGCAAATTTAAGTTAAAATCCTTATAATAATGGC
TCGACTAAGGAGAATGAAGG

Downstream 100 bases:

>100_bases
ATGAATGCAAACATTTAGTTTTTTATGATGGAGAATGTGGACTTTGTGATTCACTTGTTCAGTTTTTGATTAAAATCGAT
CATGATAAACAATTTGCCTT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 592; Mature: 591

Protein sequence:

>592_residues
MPIFNKESLENLRQRVDLVEVLSSHIELKRSGASYKGLCPFHDEKSPSFIVQKGDSHYHCFGCGAHGDAIQFLMSHQKLS
FAESVESLAQRFQVHLELVEDREEKKGVTKAFLKLALETASQFFHYCLLYSEEGHEALNYLYNRGIDLDFICHFQVGLAP
KTAGIFRKFMHAKGIKDDSLLEAGLLSVNKDGQVREFFNDRILFPIHHHSQGVIGFSGRKYKEETFGGKYINTPETSLFK
KSRVLFGLNYSRRRIAKERKAIIVEGQIDALRLIQMGFNLTVAGQGTAFGEGHVQELINLGVNQVFLALDSDLAGQEATS
KIGHLFQKEGIEVRIVQLPVGGDPDSFLREQGPEAFLELLKNSSDYLNFLIKHLSQDLNLDSPAAKNELVQKATKLIREW
DHPLMVHETLRKLAHLMKVPEEIIGVGKNHLPNIYIKKSASVGAQTIDPDRILETDLLRWLLLLGQEQTKLVEIVRTNLV
KEDFRVAICQKIYDIYRNNYENQRSCDLLSLAIDLDDAEGQLVLSDLLQKKVNKEKAEQLLIETVKKILDRNWMHKREEI
KIKVQSGHCSDDEVMELIKQFDELKRNPPIVK

Sequences:

>Translated_592_residues
MPIFNKESLENLRQRVDLVEVLSSHIELKRSGASYKGLCPFHDEKSPSFIVQKGDSHYHCFGCGAHGDAIQFLMSHQKLS
FAESVESLAQRFQVHLELVEDREEKKGVTKAFLKLALETASQFFHYCLLYSEEGHEALNYLYNRGIDLDFICHFQVGLAP
KTAGIFRKFMHAKGIKDDSLLEAGLLSVNKDGQVREFFNDRILFPIHHHSQGVIGFSGRKYKEETFGGKYINTPETSLFK
KSRVLFGLNYSRRRIAKERKAIIVEGQIDALRLIQMGFNLTVAGQGTAFGEGHVQELINLGVNQVFLALDSDLAGQEATS
KIGHLFQKEGIEVRIVQLPVGGDPDSFLREQGPEAFLELLKNSSDYLNFLIKHLSQDLNLDSPAAKNELVQKATKLIREW
DHPLMVHETLRKLAHLMKVPEEIIGVGKNHLPNIYIKKSASVGAQTIDPDRILETDLLRWLLLLGQEQTKLVEIVRTNLV
KEDFRVAICQKIYDIYRNNYENQRSCDLLSLAIDLDDAEGQLVLSDLLQKKVNKEKAEQLLIETVKKILDRNWMHKREEI
KIKVQSGHCSDDEVMELIKQFDELKRNPPIVK
>Mature_591_residues
PIFNKESLENLRQRVDLVEVLSSHIELKRSGASYKGLCPFHDEKSPSFIVQKGDSHYHCFGCGAHGDAIQFLMSHQKLSF
AESVESLAQRFQVHLELVEDREEKKGVTKAFLKLALETASQFFHYCLLYSEEGHEALNYLYNRGIDLDFICHFQVGLAPK
TAGIFRKFMHAKGIKDDSLLEAGLLSVNKDGQVREFFNDRILFPIHHHSQGVIGFSGRKYKEETFGGKYINTPETSLFKK
SRVLFGLNYSRRRIAKERKAIIVEGQIDALRLIQMGFNLTVAGQGTAFGEGHVQELINLGVNQVFLALDSDLAGQEATSK
IGHLFQKEGIEVRIVQLPVGGDPDSFLREQGPEAFLELLKNSSDYLNFLIKHLSQDLNLDSPAAKNELVQKATKLIREWD
HPLMVHETLRKLAHLMKVPEEIIGVGKNHLPNIYIKKSASVGAQTIDPDRILETDLLRWLLLLGQEQTKLVEIVRTNLVK
EDFRVAICQKIYDIYRNNYENQRSCDLLSLAIDLDDAEGQLVLSDLLQKKVNKEKAEQLLIETVKKILDRNWMHKREEIK
IKVQSGHCSDDEVMELIKQFDELKRNPPIVK

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=408, Percent_Identity=33.3333333333333, Blast_Score=228, Evalue=7e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 67481; Mature: 67349

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIFNKESLENLRQRVDLVEVLSSHIELKRSGASYKGLCPFHDEKSPSFIVQKGDSHYHC
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCEEEE
FGCGAHGDAIQFLMSHQKLSFAESVESLAQRFQVHLELVEDREEKKGVTKAFLKLALETA
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SQFFHYCLLYSEEGHEALNYLYNRGIDLDFICHFQVGLAPKTAGIFRKFMHAKGIKDDSL
HHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCCCHHH
LEAGLLSVNKDGQVREFFNDRILFPIHHHSQGVIGFSGRKYKEETFGGKYINTPETSLFK
HHHHHHCCCCCCCHHHHHCCCEEEEEECCCCCEEECCCCCCCHHHCCCCCCCCCCHHHHH
KSRVLFGLNYSRRRIAKERKAIIVEGQIDALRLIQMGFNLTVAGQGTAFGEGHVQELINL
HCCCEEECCHHHHHHHHHHCEEEEECCHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHC
GVNQVFLALDSDLAGQEATSKIGHLFQKEGIEVRIVQLPVGGDPDSFLREQGPEAFLELL
CHHHEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHCCHHHHHHHH
KNSSDYLNFLIKHLSQDLNLDSPAAKNELVQKATKLIREWDHPLMVHETLRKLAHLMKVP
HCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
EEIIGVGKNHLPNIYIKKSASVGAQTIDPDRILETDLLRWLLLLGQEQTKLVEIVRTNLV
HHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
KEDFRVAICQKIYDIYRNNYENQRSCDLLSLAIDLDDAEGQLVLSDLLQKKVNKEKAEQL
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCHHHHHHH
LIETVKKILDRNWMHKREEIKIKVQSGHCSDDEVMELIKQFDELKRNPPIVK
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PIFNKESLENLRQRVDLVEVLSSHIELKRSGASYKGLCPFHDEKSPSFIVQKGDSHYHC
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCEEEE
FGCGAHGDAIQFLMSHQKLSFAESVESLAQRFQVHLELVEDREEKKGVTKAFLKLALETA
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SQFFHYCLLYSEEGHEALNYLYNRGIDLDFICHFQVGLAPKTAGIFRKFMHAKGIKDDSL
HHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCCCHHH
LEAGLLSVNKDGQVREFFNDRILFPIHHHSQGVIGFSGRKYKEETFGGKYINTPETSLFK
HHHHHHCCCCCCCHHHHHCCCEEEEEECCCCCEEECCCCCCCHHHCCCCCCCCCCHHHHH
KSRVLFGLNYSRRRIAKERKAIIVEGQIDALRLIQMGFNLTVAGQGTAFGEGHVQELINL
HCCCEEECCHHHHHHHHHHCEEEEECCHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHC
GVNQVFLALDSDLAGQEATSKIGHLFQKEGIEVRIVQLPVGGDPDSFLREQGPEAFLELL
CHHHEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHCCHHHHHHHH
KNSSDYLNFLIKHLSQDLNLDSPAAKNELVQKATKLIREWDHPLMVHETLRKLAHLMKVP
HCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
EEIIGVGKNHLPNIYIKKSASVGAQTIDPDRILETDLLRWLLLLGQEQTKLVEIVRTNLV
HHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
KEDFRVAICQKIYDIYRNNYENQRSCDLLSLAIDLDDAEGQLVLSDLLQKKVNKEKAEQL
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCHHHHHHH
LIETVKKILDRNWMHKREEIKIKVQSGHCSDDEVMELIKQFDELKRNPPIVK
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]