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The map label for this gene is nuoF [H]

Identifier: 46446198

GI number: 46446198

Start: 696846

End: 698144

Strand: Direct

Name: nuoF [H]

Synonym: pc0564

Alternate gene names: 46446198

Gene position: 696846-698144 (Clockwise)

Preceding gene: 46446197

Following gene: 46446199

Centisome position: 28.86

GC content: 37.34

Gene sequence:

>1299_bases
ATGTCTGAAATGCGTATTTTAACAGCTTACATGGATGATCCACATCAATGTAAGATCGAAACTTATGAGCGTAATGGCGG
TTATCAAGCACTCAAAAAAGCAATTTCTTCTATTTCCTCGGATCAGCTAATTGAAATGGTAAAACAATCTTGGTTGAGAG
GTAGAGGTGGAGGTGGTTTTCAAACAGGCTTGAAATGGAGTTTTGTCCCCAAAGATTGTCAAATTTCTAAATATCTCGTT
TGCAATTGTGATGAAAGTGAACCTGGTACATTTAAAGATCGATATATCATTGAAAATGATCCTCATCAATTAATTGAAGG
AATAATTTTAGCTTGTTACGCAATAGGGGCAAAGCAGGCTTTTATTTATTGCAGAGGAGAATTTTTTGAAGGAAATAAAA
AGTTGCGATTAGCTATTCAAGAAGCTAAAAAAAGAGGTTATTTAGAAGCTCCATTAGGAGAAGCAAATTTTTCTGTTAGT
ATTATTGTTCATCCAGGCGCAGGAGCCTATATTGCAGGCGAAGAAACTGCTCAATTGAATTCACTTGAAGGCTATCGTGC
AACTCCTCGTTTAAAACCCCCTTTTCCAGCAGTTTCTGGCTTATATGAAAAACCGACTGTCGTTAATAATGTTGAAACAT
TATGTAACGTTGTTCACATTGTGAATCGTGGAGTTGAATGGTATCAAAGTATTGGTAAACCAAAAAATACAGGAACAAAA
ATCTTCCAAGTTAGCGGTCATGTTCAAAATCCAGGATGTTTTGAATTTCCTTTGGGAATTCCTTTAAAAGAAGTTTTAGA
AAGAGCTGGTTGGATGCTACCAGGCCATCAATTTAAAGCTTGTTATCCGGGGGGATCATCTTGTTCATTATTGACTGAAC
GGGATTTAGATACTCCTATGGATTTTGATAGTGTTGCAGCAAAAAAATCCATGTTGGGAACGGCATCAATTATTGTTATG
GATGATTCGACGGATATGGTGAAAGTTGCCAATCGTTTGATGGAATTCTATCAAAATGAGTCTTGTGGAAAATGTACTCC
TTGTCGCGAAGGGACGCGTTGGACAAAACAAATCCTTACTCGTATCTTAGCGGGTAGGGGAACAGTTGGGGATTTAAAAA
CGATAGAGCGAATTTGTCAACAAATGGAAATGGATTCGTTTTGCCCGTTGGCTCCAGGAGCTGCTCTTCCAATTGTCAGT
GCGATTAGAGAATTCAGAGGCGAATTTGAATCTTATATTATGCGTAACATTCATGCTGATAAACCTCCTGAAATGAAAAT
TACTTATCCTTATTTGTAG

Upstream 100 bases:

>100_bases
GTCATTGGACCAGTAGAAATCACACAAATCGATGATTACATTCAAAAAGCCAAACAAAGCTATGGCCATCACTCTCCGAT
CACCATTAAGGAGGTAGAAG

Downstream 100 bases:

>100_bases
GTAGTGACTGTGGAAATGATGATTAACTTAATAATCGATGGAAAGGAATTAAGTGTTCCAAAGGGAACAACTGTTTATCA
TGCCGCCAAACAACTAGGCA

Product: NADH-ubiquinone oxidoreductase chain F

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MSEMRILTAYMDDPHQCKIETYERNGGYQALKKAISSISSDQLIEMVKQSWLRGRGGGGFQTGLKWSFVPKDCQISKYLV
CNCDESEPGTFKDRYIIENDPHQLIEGIILACYAIGAKQAFIYCRGEFFEGNKKLRLAIQEAKKRGYLEAPLGEANFSVS
IIVHPGAGAYIAGEETAQLNSLEGYRATPRLKPPFPAVSGLYEKPTVVNNVETLCNVVHIVNRGVEWYQSIGKPKNTGTK
IFQVSGHVQNPGCFEFPLGIPLKEVLERAGWMLPGHQFKACYPGGSSCSLLTERDLDTPMDFDSVAAKKSMLGTASIIVM
DDSTDMVKVANRLMEFYQNESCGKCTPCREGTRWTKQILTRILAGRGTVGDLKTIERICQQMEMDSFCPLAPGAALPIVS
AIREFRGEFESYIMRNIHADKPPEMKITYPYL

Sequences:

>Translated_432_residues
MSEMRILTAYMDDPHQCKIETYERNGGYQALKKAISSISSDQLIEMVKQSWLRGRGGGGFQTGLKWSFVPKDCQISKYLV
CNCDESEPGTFKDRYIIENDPHQLIEGIILACYAIGAKQAFIYCRGEFFEGNKKLRLAIQEAKKRGYLEAPLGEANFSVS
IIVHPGAGAYIAGEETAQLNSLEGYRATPRLKPPFPAVSGLYEKPTVVNNVETLCNVVHIVNRGVEWYQSIGKPKNTGTK
IFQVSGHVQNPGCFEFPLGIPLKEVLERAGWMLPGHQFKACYPGGSSCSLLTERDLDTPMDFDSVAAKKSMLGTASIIVM
DDSTDMVKVANRLMEFYQNESCGKCTPCREGTRWTKQILTRILAGRGTVGDLKTIERICQQMEMDSFCPLAPGAALPIVS
AIREFRGEFESYIMRNIHADKPPEMKITYPYL
>Mature_431_residues
SEMRILTAYMDDPHQCKIETYERNGGYQALKKAISSISSDQLIEMVKQSWLRGRGGGGFQTGLKWSFVPKDCQISKYLVC
NCDESEPGTFKDRYIIENDPHQLIEGIILACYAIGAKQAFIYCRGEFFEGNKKLRLAIQEAKKRGYLEAPLGEANFSVSI
IVHPGAGAYIAGEETAQLNSLEGYRATPRLKPPFPAVSGLYEKPTVVNNVETLCNVVHIVNRGVEWYQSIGKPKNTGTKI
FQVSGHVQNPGCFEFPLGIPLKEVLERAGWMLPGHQFKACYPGGSSCSLLTERDLDTPMDFDSVAAKKSMLGTASIIVMD
DSTDMVKVANRLMEFYQNESCGKCTPCREGTRWTKQILTRILAGRGTVGDLKTIERICQQMEMDSFCPLAPGAALPIVSA
IREFRGEFESYIMRNIHADKPPEMKITYPYL

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260656005, Length=380, Percent_Identity=45.5263157894737, Blast_Score=331, Evalue=8e-91,
Organism=Homo sapiens, GI20149568, Length=380, Percent_Identity=45.5263157894737, Blast_Score=331, Evalue=8e-91,
Organism=Escherichia coli, GI1788620, Length=405, Percent_Identity=48.1481481481481, Blast_Score=394, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17531727, Length=405, Percent_Identity=44.1975308641975, Blast_Score=328, Evalue=4e-90,
Organism=Drosophila melanogaster, GI19920794, Length=429, Percent_Identity=42.6573426573427, Blast_Score=337, Evalue=8e-93,
Organism=Drosophila melanogaster, GI45550452, Length=412, Percent_Identity=41.747572815534, Blast_Score=319, Evalue=2e-87,
Organism=Drosophila melanogaster, GI24653819, Length=381, Percent_Identity=34.6456692913386, Blast_Score=233, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24653817, Length=357, Percent_Identity=33.3333333333333, Blast_Score=208, Evalue=6e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 48168; Mature: 48037

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEMRILTAYMDDPHQCKIETYERNGGYQALKKAISSISSDQLIEMVKQSWLRGRGGGGF
CCCCEEEEEECCCCCEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCC
QTGLKWSFVPKDCQISKYLVCNCDESEPGTFKDRYIIENDPHQLIEGIILACYAIGAKQA
CCCCEEEECCCCCCCCEEEEEECCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCEE
FIYCRGEFFEGNKKLRLAIQEAKKRGYLEAPLGEANFSVSIIVHPGAGAYIAGEETAQLN
EEEEECEEECCCCEEEHEEHHHHHCCCEECCCCCCCEEEEEEEECCCCEEECCCCHHHHC
SLEGYRATPRLKPPFPAVSGLYEKPTVVNNVETLCNVVHIVNRGVEWYQSIGKPKNTGTK
CCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCE
IFQVSGHVQNPGCFEFPLGIPLKEVLERAGWMLPGHQFKACYPGGSSCSLLTERDLDTPM
EEEEECCCCCCCCEECCCCCCHHHHHHHCCCCCCCCCEEEECCCCCCCEEEECCCCCCCC
DFDSVAAKKSMLGTASIIVMDDSTDMVKVANRLMEFYQNESCGKCTPCREGTRWTKQILT
CHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
RILAGRGTVGDLKTIERICQQMEMDSFCPLAPGAALPIVSAIREFRGEFESYIMRNIHAD
HHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
KPPEMKITYPYL
CCCCEEEECCCC
>Mature Secondary Structure 
SEMRILTAYMDDPHQCKIETYERNGGYQALKKAISSISSDQLIEMVKQSWLRGRGGGGF
CCCEEEEEECCCCCEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCC
QTGLKWSFVPKDCQISKYLVCNCDESEPGTFKDRYIIENDPHQLIEGIILACYAIGAKQA
CCCCEEEECCCCCCCCEEEEEECCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCEE
FIYCRGEFFEGNKKLRLAIQEAKKRGYLEAPLGEANFSVSIIVHPGAGAYIAGEETAQLN
EEEEECEEECCCCEEEHEEHHHHHCCCEECCCCCCCEEEEEEEECCCCEEECCCCHHHHC
SLEGYRATPRLKPPFPAVSGLYEKPTVVNNVETLCNVVHIVNRGVEWYQSIGKPKNTGTK
CCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCE
IFQVSGHVQNPGCFEFPLGIPLKEVLERAGWMLPGHQFKACYPGGSSCSLLTERDLDTPM
EEEEECCCCCCCCEECCCCCCHHHHHHHCCCCCCCCCEEEECCCCCCCEEEECCCCCCCC
DFDSVAAKKSMLGTASIIVMDDSTDMVKVANRLMEFYQNESCGKCTPCREGTRWTKQILT
CHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
RILAGRGTVGDLKTIERICQQMEMDSFCPLAPGAALPIVSAIREFRGEFESYIMRNIHAD
HHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
KPPEMKITYPYL
CCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]