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The map label for this gene is nuoE [H]

Identifier: 46446197

GI number: 46446197

Start: 696374

End: 696853

Strand: Direct

Name: nuoE [H]

Synonym: pc0563

Alternate gene names: 46446197

Gene position: 696374-696853 (Clockwise)

Preceding gene: 46446196

Following gene: 46446198

Centisome position: 28.84

GC content: 36.88

Gene sequence:

>480_bases
TTGCAGAATCTATATCCAAATAAACGCTCAGCTCTTATTCCAGCCCTTCATTTAGCACAAGCTGAAAAAGGATATTTACC
AATTGAAGTTCAAAATGAACTTGCCTTCTTATTTGATTTAGAACCAAGTGAAGTTAATTCTATTGTCACTTTTTATGATA
TGTTTTTTGAAGAACCTGTCGGAAAACATGTGATTCATGTTTGCAAGAATATTTCTTGCATGTTAAGAGGCGCTGATGGA
TTTCTAGCGAGATTATGTCAAAAAATGCATATTTCTCCAGGTGAAACGACGCAGGACGGTGAGTTTACGGTGATTGCTTC
AGAATGTTTAGCAGCTTGTGATAAAGCTCCAGTGATGATTGTTGATGATAAAGTCATTGGACCAGTAGAAATCACACAAA
TCGATGATTACATTCAAAAAGCCAAACAAAGCTATGGCCATCACTCTCCGATCACCATTAAGGAGGTAGAAGATGTCTGA

Upstream 100 bases:

>100_bases
TCATTTCAGATGTTGTTGTTGCTATTGCTAGTGTTGACCCTATACTTGGAGACGTAGATCGCTAATGCTTACCGAAGAAA
CAAAACAGGCTATCTTAAAA

Downstream 100 bases:

>100_bases
AATGCGTATTTTAACAGCTTACATGGATGATCCACATCAATGTAAGATCGAAACTTATGAGCGTAATGGCGGTTATCAAG
CACTCAAAAAAGCAATTTCT

Product: putative NADH-ubiquinone oxidoreductase chain E

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E 2; NDH-1 subunit E 2 [H]

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MQNLYPNKRSALIPALHLAQAEKGYLPIEVQNELAFLFDLEPSEVNSIVTFYDMFFEEPVGKHVIHVCKNISCMLRGADG
FLARLCQKMHISPGETTQDGEFTVIASECLAACDKAPVMIVDDKVIGPVEITQIDDYIQKAKQSYGHHSPITIKEVEDV

Sequences:

>Translated_159_residues
MQNLYPNKRSALIPALHLAQAEKGYLPIEVQNELAFLFDLEPSEVNSIVTFYDMFFEEPVGKHVIHVCKNISCMLRGADG
FLARLCQKMHISPGETTQDGEFTVIASECLAACDKAPVMIVDDKVIGPVEITQIDDYIQKAKQSYGHHSPITIKEVEDV
>Mature_159_residues
MQNLYPNKRSALIPALHLAQAEKGYLPIEVQNELAFLFDLEPSEVNSIVTFYDMFFEEPVGKHVIHVCKNISCMLRGADG
FLARLCQKMHISPGETTQDGEFTVIASECLAACDKAPVMIVDDKVIGPVEITQIDDYIQKAKQSYGHHSPITIKEVEDV

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=140, Percent_Identity=39.2857142857143, Blast_Score=107, Evalue=6e-24,
Organism=Escherichia coli, GI1788621, Length=138, Percent_Identity=34.7826086956522, Blast_Score=104, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17561328, Length=148, Percent_Identity=37.1621621621622, Blast_Score=109, Evalue=8e-25,
Organism=Drosophila melanogaster, GI18859877, Length=123, Percent_Identity=40.650406504065, Blast_Score=104, Evalue=3e-23,
Organism=Drosophila melanogaster, GI21355353, Length=115, Percent_Identity=36.5217391304348, Blast_Score=90, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 17752; Mature: 17752

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS01099 COMPLEX1_24K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNLYPNKRSALIPALHLAQAEKGYLPIEVQNELAFLFDLEPSEVNSIVTFYDMFFEEPV
CCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCEEEEEECCHHHHHHHHHHHHHHHHCCH
GKHVIHVCKNISCMLRGADGFLARLCQKMHISPGETTQDGEFTVIASECLAACDKAPVMI
HHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHCCCCCEEE
VDDKVIGPVEITQIDDYIQKAKQSYGHHSPITIKEVEDV
ECCCCCCCEEHHHHHHHHHHHHHHCCCCCCEEEEECCCC
>Mature Secondary Structure
MQNLYPNKRSALIPALHLAQAEKGYLPIEVQNELAFLFDLEPSEVNSIVTFYDMFFEEPV
CCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCEEEEEECCHHHHHHHHHHHHHHHHCCH
GKHVIHVCKNISCMLRGADGFLARLCQKMHISPGETTQDGEFTVIASECLAACDKAPVMI
HHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHCCCCCEEE
VDDKVIGPVEITQIDDYIQKAKQSYGHHSPITIKEVEDV
ECCCCCCCEEHHHHHHHHHHHHHHCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]