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The map label for this gene is cobA

Identifier: 45659188

GI number: 45659188

Start: 4131865

End: 4132704

Strand: Reverse

Name: cobA

Synonym: LIC13369

Alternate gene names: 45659188

Gene position: 4132704-4131865 (Counterclockwise)

Preceding gene: 45659189

Following gene: 45659187

Centisome position: 96.62

GC content: 39.88

Gene sequence:

>840_bases
GTGTTAAACGCTGATACAAATAAAGATTCTCGTCCCGGCAAAGTTTACCTTGTTGGGGCCGGTCCGGGAAATCCGGAAGA
TCTGACTTTGCGCGCGTATCGAATTTTAACCAAAGCCGAAATTATTTTGTACGACGCTCTTTTAGACCCTTCTTTTCTCG
AAATTTTTCCAAAGTCCGCGATCGTTCACTACGTCGGAAAAAGATCCGGAGCACATTCCGCGACTCAAGAGGAGATCAAT
CAACTTCTTCTTTCTTATGCTCTTTCCGGAAAAAACGTAGTTCGTTTGAAAGGAGGAGATCCTTTTGTTTTTGGAAGAGG
TGGCGAAGAATTAATTACATTAGTTAATCATAATATAAAATATGAAATTGTTCCAGGCGTAAGCTCTTTGAACGCAGGTT
CTTCTTTTGCAGGTTTTCCTTTGACTCATAGGGGTTTGTCCCGCCAGGTTTTGATCATGGACGGTCATACGGTTCTGAAC
GAGGAAACTGATTGGGAGTGGTTTGCCAAATTCAAAGGAACGATTGCGCTTTTTATGGGAACCTCTTCCCTTCCAAAAAT
CGCGAATTTACTTTTACAACATGGTAGTTCGGCCGATCTGCCGGTTGCACTTGTTGAAAATGCTTCTTTAGAAAATTTTA
ATATTCAAATCTGTTCTTTAAAAGAAGCTGCAAATTCTTATTTGGAAAAGAAAACACAAGGCCCTGGGATTATTTACATT
GGAAAAGTGGTTCGATTTTTGGAAAATAAGAAATTGACTTCGACCTTTTCTCAGTTTTCGAAAGAATCTTTTTTAGGGCA
AATTTCTTCCGAGATCCAAGGAGGAAAGAATGAGTCGTAA

Upstream 100 bases:

>100_bases
GTATCGAATCAATCGTTCTACGGGAAGTTTTATCTTGGTGGATGAAGGGACTAATTCTACTGTAGGCGCGGGGATGATTA
CCGGAGTCGGAGCCTAATCC

Downstream 100 bases:

>100_bases
GTATCCGGCTTTTCTTAATTTAGAAAATAAGAATGTTCTGTTAATTGGTGGAGGAAAAGTCGCCTTAGAAAAGCTTCCTC
ATCTGATCGATTCCGGTGCA

Product: uroporphyrinogen-III C-methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MLNADTNKDSRPGKVYLVGAGPGNPEDLTLRAYRILTKAEIILYDALLDPSFLEIFPKSAIVHYVGKRSGAHSATQEEIN
QLLLSYALSGKNVVRLKGGDPFVFGRGGEELITLVNHNIKYEIVPGVSSLNAGSSFAGFPLTHRGLSRQVLIMDGHTVLN
EETDWEWFAKFKGTIALFMGTSSLPKIANLLLQHGSSADLPVALVENASLENFNIQICSLKEAANSYLEKKTQGPGIIYI
GKVVRFLENKKLTSTFSQFSKESFLGQISSEIQGGKNES

Sequences:

>Translated_279_residues
MLNADTNKDSRPGKVYLVGAGPGNPEDLTLRAYRILTKAEIILYDALLDPSFLEIFPKSAIVHYVGKRSGAHSATQEEIN
QLLLSYALSGKNVVRLKGGDPFVFGRGGEELITLVNHNIKYEIVPGVSSLNAGSSFAGFPLTHRGLSRQVLIMDGHTVLN
EETDWEWFAKFKGTIALFMGTSSLPKIANLLLQHGSSADLPVALVENASLENFNIQICSLKEAANSYLEKKTQGPGIIYI
GKVVRFLENKKLTSTFSQFSKESFLGQISSEIQGGKNES
>Mature_279_residues
MLNADTNKDSRPGKVYLVGAGPGNPEDLTLRAYRILTKAEIILYDALLDPSFLEIFPKSAIVHYVGKRSGAHSATQEEIN
QLLLSYALSGKNVVRLKGGDPFVFGRGGEELITLVNHNIKYEIVPGVSSLNAGSSFAGFPLTHRGLSRQVLIMDGHTVLN
EETDWEWFAKFKGTIALFMGTSSLPKIANLLLQHGSSADLPVALVENASLENFNIQICSLKEAANSYLEKKTQGPGIIYI
GKVVRFLENKKLTSTFSQFSKESFLGQISSEIQGGKNES

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=232, Percent_Identity=41.3793103448276, Blast_Score=192, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6322922, Length=240, Percent_Identity=33.3333333333333, Blast_Score=117, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043 [H]

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4 [H]

Molecular weight: Translated: 30449; Mature: 30449

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNADTNKDSRPGKVYLVGAGPGNPEDLTLRAYRILTKAEIILYDALLDPSFLEIFPKSA
CCCCCCCCCCCCCEEEEEECCCCCCCCEEEHHHHHHHHHHEEEEEHHCCCHHHHHCCHHH
IVHYVGKRSGAHSATQEEINQLLLSYALSGKNVVRLKGGDPFVFGRGGEELITLVNHNIK
HHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCEEEECCCCHHHHHEECCCEE
YEIVPGVSSLNAGSSFAGFPLTHRGLSRQVLIMDGHTVLNEETDWEWFAKFKGTIALFMG
EEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCEEECCCCCHHHHHHHCCEEEEEEC
TSSLPKIANLLLQHGSSADLPVALVENASLENFNIQICSLKEAANSYLEKKTQGPGIIYI
CCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCEEEH
GKVVRFLENKKLTSTFSQFSKESFLGQISSEIQGGKNES
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLNADTNKDSRPGKVYLVGAGPGNPEDLTLRAYRILTKAEIILYDALLDPSFLEIFPKSA
CCCCCCCCCCCCCEEEEEECCCCCCCCEEEHHHHHHHHHHEEEEEHHCCCHHHHHCCHHH
IVHYVGKRSGAHSATQEEINQLLLSYALSGKNVVRLKGGDPFVFGRGGEELITLVNHNIK
HHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCEEEECCCCHHHHHEECCCEE
YEIVPGVSSLNAGSSFAGFPLTHRGLSRQVLIMDGHTVLNEETDWEWFAKFKGTIALFMG
EEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCEEECCCCCHHHHHHHCCEEEEEEC
TSSLPKIANLLLQHGSSADLPVALVENASLENFNIQICSLKEAANSYLEKKTQGPGIIYI
CCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCEEEH
GKVVRFLENKKLTSTFSQFSKESFLGQISSEIQGGKNES
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA