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The map label for this gene is cysN

Identifier: 45659189

GI number: 45659189

Start: 4132708

End: 4133958

Strand: Reverse

Name: cysN

Synonym: LIC13370

Alternate gene names: 45659189

Gene position: 4133958-4132708 (Counterclockwise)

Preceding gene: 45659190

Following gene: 45659188

Centisome position: 96.65

GC content: 39.89

Gene sequence:

>1251_bases
ATGGATTTATTACGTTTTATCACTGCAGGAAGTGTGGACGACGGTAAATCCACTTTGATCGGAAGACTTCTCTACGACAG
CAAATCGATCTTCGAAGATCAGCTCGAAGCGATTGAAAAAAACGCTCGTGGAAACAACGGGCAGATCAATCTGGCTCTTC
TTACTGATGGTCTCAAAGCCGAAAGAGAACAGGGAATTACGATTGATGTAGCTTATAAGTATTTTTCCACTCCCAAAAGA
AAGTTCATTATTGCGGACGCACCCGGACATGTTCAATACACTCGGAACATGGTGACTGGAGCGTCTAACTCTAATTTGGC
GATTATTTTGATAGATGCTCGAAAAGGTGTAATCGAACAAACCTATCGTCATTCTTACATTACATCTATGTTAAGAATCC
CTCATCTTGTAGTTTGTATCAATAAAATGGATCTTGTGGAATTTTCCCAAGCTCGTTACGAAGAGATCAAAACGGATTAT
TTAAATTTTGCATCTCGTTTAGATATTAAGGATATTGAATTTATTCCGATCAGTGCTCTCAACGGAGACAACGTAGTCGA
CAAGTCCGAAAATCTTTCCTGGTATGATGGGCGCACTCTTCTCAATCATCTTGAAGAAGTTTATATCGAAAGCGATGAGA
ACATAGAAGACCCACGCTTTCCGGTTCAGTATGTGATCCGTCCTCTTTCCGATGAACATCACGATTATAGAGGTTATGCG
GGTCAGGTTCGAAGTGGTATTTTTAGAAAGGGAGATTCTATTACGGTTCTTCCAAGTGGTTTTACAAGTACGATCGAATC
TATCAATACTTACGATGGAGAAGTGGAAGAGGCATTTCCTCCTATGTCGGTTACAATCCGTTTAAAAGATGAGATTGATA
TTTCTCGTGGTGATATGATCGTTTTATCTGAAAGTCTTCCAATTGTTTCCCAAGATTTAGAAGCAAATATTTGTTGGATG
GATTCCAAGGCCCTTTTATCTGGAAATAAATATATTCTGCGTCAAACAACTAACACTGTAAAAGCGATCGTAAAAGAAAT
CGAATTTAAAGTTGCCCCGGATACACACGAAAAATTGGACGCTTCTAAGGGACTTACGTTAAACGAAATTGGAAAGATTA
AAATTCGCACTGCAAAACCAGTGAGTTTCGACGAGTATCGAATCAATCGTTCTACGGGAAGTTTTATCTTGGTGGATGAA
GGGACTAATTCTACTGTAGGCGCGGGGATGATTACCGGAGTCGGAGCCTAA

Upstream 100 bases:

>100_bases
TCGAAATCCAAACGACTCGGACCACAGAGAGAGGTTCCAGACTAGACGATAAACGCTCCGAAGCCGCTATGGAAGATAGA
AAAAGAGGAGGTTACTTCTG

Downstream 100 bases:

>100_bases
TCCGTGTTAAACGCTGATACAAATAAAGATTCTCGTCCCGGCAAAGTTTACCTTGTTGGGGCCGGTCCGGGAAATCCGGA
AGATCTGACTTTGCGCGCGT

Product: sulfate adenylyltransferase subunit 1

Products: NA

Alternate protein names: Sulfate adenylyltransferase subunit 1; ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT; Adenylyl-sulfate kinase; APS kinase; ATP adenosine-5'-phosphosulfate 3'-phosphotransferase [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MDLLRFITAGSVDDGKSTLIGRLLYDSKSIFEDQLEAIEKNARGNNGQINLALLTDGLKAEREQGITIDVAYKYFSTPKR
KFIIADAPGHVQYTRNMVTGASNSNLAIILIDARKGVIEQTYRHSYITSMLRIPHLVVCINKMDLVEFSQARYEEIKTDY
LNFASRLDIKDIEFIPISALNGDNVVDKSENLSWYDGRTLLNHLEEVYIESDENIEDPRFPVQYVIRPLSDEHHDYRGYA
GQVRSGIFRKGDSITVLPSGFTSTIESINTYDGEVEEAFPPMSVTIRLKDEIDISRGDMIVLSESLPIVSQDLEANICWM
DSKALLSGNKYILRQTTNTVKAIVKEIEFKVAPDTHEKLDASKGLTLNEIGKIKIRTAKPVSFDEYRINRSTGSFILVDE
GTNSTVGAGMITGVGA

Sequences:

>Translated_416_residues
MDLLRFITAGSVDDGKSTLIGRLLYDSKSIFEDQLEAIEKNARGNNGQINLALLTDGLKAEREQGITIDVAYKYFSTPKR
KFIIADAPGHVQYTRNMVTGASNSNLAIILIDARKGVIEQTYRHSYITSMLRIPHLVVCINKMDLVEFSQARYEEIKTDY
LNFASRLDIKDIEFIPISALNGDNVVDKSENLSWYDGRTLLNHLEEVYIESDENIEDPRFPVQYVIRPLSDEHHDYRGYA
GQVRSGIFRKGDSITVLPSGFTSTIESINTYDGEVEEAFPPMSVTIRLKDEIDISRGDMIVLSESLPIVSQDLEANICWM
DSKALLSGNKYILRQTTNTVKAIVKEIEFKVAPDTHEKLDASKGLTLNEIGKIKIRTAKPVSFDEYRINRSTGSFILVDE
GTNSTVGAGMITGVGA
>Mature_416_residues
MDLLRFITAGSVDDGKSTLIGRLLYDSKSIFEDQLEAIEKNARGNNGQINLALLTDGLKAEREQGITIDVAYKYFSTPKR
KFIIADAPGHVQYTRNMVTGASNSNLAIILIDARKGVIEQTYRHSYITSMLRIPHLVVCINKMDLVEFSQARYEEIKTDY
LNFASRLDIKDIEFIPISALNGDNVVDKSENLSWYDGRTLLNHLEEVYIESDENIEDPRFPVQYVIRPLSDEHHDYRGYA
GQVRSGIFRKGDSITVLPSGFTSTIESINTYDGEVEEAFPPMSVTIRLKDEIDISRGDMIVLSESLPIVSQDLEANICWM
DSKALLSGNKYILRQTTNTVKAIVKEIEFKVAPDTHEKLDASKGLTLNEIGKIKIRTAKPVSFDEYRINRSTGSFILVDE
GTNSTVGAGMITGVGA

Specific function: APS kinase catalyzes the synthesis of activated sulfate [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the APS kinase family [H]

Homologues:

Organism=Homo sapiens, GI4503471, Length=437, Percent_Identity=29.2906178489703, Blast_Score=166, Evalue=5e-41,
Organism=Homo sapiens, GI194018522, Length=422, Percent_Identity=29.1469194312796, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI4503475, Length=437, Percent_Identity=29.2906178489703, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI194018520, Length=422, Percent_Identity=29.1469194312796, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI194097354, Length=422, Percent_Identity=29.1469194312796, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI223555963, Length=429, Percent_Identity=28.4382284382284, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI5729864, Length=429, Percent_Identity=28.4382284382284, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI46094014, Length=422, Percent_Identity=28.436018957346, Blast_Score=158, Evalue=9e-39,
Organism=Homo sapiens, GI34147630, Length=324, Percent_Identity=25.6172839506173, Blast_Score=76, Evalue=6e-14,
Organism=Escherichia coli, GI1789108, Length=414, Percent_Identity=50.9661835748792, Blast_Score=397, Evalue=1e-112,
Organism=Escherichia coli, GI1790412, Length=138, Percent_Identity=28.9855072463768, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1789737, Length=138, Percent_Identity=28.9855072463768, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI2367247, Length=153, Percent_Identity=32.0261437908497, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17552884, Length=437, Percent_Identity=30.6636155606407, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17569207, Length=437, Percent_Identity=30.6636155606407, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI32566303, Length=430, Percent_Identity=30, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI32566629, Length=424, Percent_Identity=26.8867924528302, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI115532067, Length=286, Percent_Identity=32.1678321678322, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI115532065, Length=286, Percent_Identity=32.1678321678322, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI32566301, Length=142, Percent_Identity=37.3239436619718, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25141371, Length=278, Percent_Identity=26.6187050359712, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=313, Percent_Identity=26.517571884984, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6325337, Length=435, Percent_Identity=29.6551724137931, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6319594, Length=435, Percent_Identity=29.6551724137931, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6322937, Length=361, Percent_Identity=31.0249307479224, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6320377, Length=433, Percent_Identity=30.9468822170901, Blast_Score=141, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6324761, Length=161, Percent_Identity=32.2981366459627, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24652838, Length=454, Percent_Identity=28.1938325991189, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI17137572, Length=454, Percent_Identity=28.1938325991189, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI45553807, Length=437, Percent_Identity=28.3752860411899, Blast_Score=157, Evalue=9e-39,
Organism=Drosophila melanogaster, GI45553816, Length=437, Percent_Identity=28.3752860411899, Blast_Score=157, Evalue=9e-39,
Organism=Drosophila melanogaster, GI24651721, Length=437, Percent_Identity=28.3752860411899, Blast_Score=157, Evalue=9e-39,
Organism=Drosophila melanogaster, GI17864154, Length=437, Percent_Identity=28.3752860411899, Blast_Score=157, Evalue=9e-39,
Organism=Drosophila melanogaster, GI17137380, Length=429, Percent_Identity=28.4382284382284, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI45550900, Length=435, Percent_Identity=27.5862068965517, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI281363316, Length=317, Percent_Identity=26.1829652996845, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17864358, Length=317, Percent_Identity=26.1829652996845, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19921738, Length=164, Percent_Identity=29.8780487804878, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002891
- InterPro:   IPR000795
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF01583 APS_kinase; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4; =2.7.1.25 [H]

Molecular weight: Translated: 46537; Mature: 46537

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLLRFITAGSVDDGKSTLIGRLLYDSKSIFEDQLEAIEKNARGNNGQINLALLTDGLKA
CCHHHEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHH
EREQGITIDVAYKYFSTPKRKFIIADAPGHVQYTRNMVTGASNSNLAIILIDARKGVIEQ
HHHCCCEEEEEEHHHCCCCEEEEEECCCCCEEEEHHEEECCCCCCEEEEEEECCCCHHHH
TYRHSYITSMLRIPHLVVCINKMDLVEFSQARYEEIKTDYLNFASRLDIKDIEFIPISAL
HHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEC
NGDNVVDKSENLSWYDGRTLLNHLEEVYIESDENIEDPRFPVQYVIRPLSDEHHDYRGYA
CCCCCCCCCCCCCEECHHHHHHHHHHHEECCCCCCCCCCCCHHHHHCCCCCCCCCCCCCH
GQVRSGIFRKGDSITVLPSGFTSTIESINTYDGEVEEAFPPMSVTIRLKDEIDISRGDMI
HHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCCCHHHCCCCEEEEEEEECCCCCCCCCEE
VLSESLPIVSQDLEANICWMDSKALLSGNKYILRQTTNTVKAIVKEIEFKVAPDTHEKLD
EEECCCCCEECCCCCEEEEECCHHHHCCCEEEEEECHHHHHHHHHHHEEEECCCCHHHHH
ASKGLTLNEIGKIKIRTAKPVSFDEYRINRSTGSFILVDEGTNSTVGAGMITGVGA
HHCCCCHHCCCEEEEEECCCCCCCCEEEECCCCCEEEEECCCCCCCCCCCEECCCC
>Mature Secondary Structure
MDLLRFITAGSVDDGKSTLIGRLLYDSKSIFEDQLEAIEKNARGNNGQINLALLTDGLKA
CCHHHEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHH
EREQGITIDVAYKYFSTPKRKFIIADAPGHVQYTRNMVTGASNSNLAIILIDARKGVIEQ
HHHCCCEEEEEEHHHCCCCEEEEEECCCCCEEEEHHEEECCCCCCEEEEEEECCCCHHHH
TYRHSYITSMLRIPHLVVCINKMDLVEFSQARYEEIKTDYLNFASRLDIKDIEFIPISAL
HHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEC
NGDNVVDKSENLSWYDGRTLLNHLEEVYIESDENIEDPRFPVQYVIRPLSDEHHDYRGYA
CCCCCCCCCCCCCEECHHHHHHHHHHHEECCCCCCCCCCCCHHHHHCCCCCCCCCCCCCH
GQVRSGIFRKGDSITVLPSGFTSTIESINTYDGEVEEAFPPMSVTIRLKDEIDISRGDMI
HHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCCCHHHCCCCEEEEEEEECCCCCCCCCEE
VLSESLPIVSQDLEANICWMDSKALLSGNKYILRQTTNTVKAIVKEIEFKVAPDTHEKLD
EEECCCCCEECCCCCEEEEECCHHHHCCCEEEEEECHHHHHHHHHHHEEEECCCCHHHHH
ASKGLTLNEIGKIKIRTAKPVSFDEYRINRSTGSFILVDEGTNSTVGAGMITGVGA
HHCCCCHHCCCEEEEEECCCCCCCCEEEECCCCCEEEEECCCCCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]