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The map label for this gene is narK1 [H]

Identifier: 38637929

GI number: 38637929

Start: 284442

End: 285722

Strand: Direct

Name: narK1 [H]

Synonym: PHG268

Alternate gene names: 38637929

Gene position: 284442-285722 (Clockwise)

Preceding gene: 38637927

Following gene: 38637930

Centisome position: 62.91

GC content: 66.51

Gene sequence:

>1281_bases
ATGGCCACCCCACATCCCGCGCGGGCGCCGATCCCGCCCGGTGCCTATACGACGCTGGCATCCAGCACTTTCGCCTTCAC
CATCTGCTTTGCCGTCTGGATGCTGTTCGCGGTGCTCGGCATTCCGCTCAAGAAGGAACTCGGCCTGAACGACACCGAGT
TCGGGCTGCTGGCGGCAACGCCAGTCCTGAGCGGCTCGCTGATCCGCGTGCCGCTAGGCCTCTGGACCGACCGCTACGGC
GGCCGCATCGTCTTCTTCGTGCTGATGCTGGCCACCGTGATCCCGATCTGGCTGATCTCGTACGCGCACAGCCTGTGGCA
ACTGCTGGTGCTGGGCCTGTTCGTCGGCCTGGCCGGCGGCTCATTCTCGGTGGGCACGCCGTATGTGGCGCGCTGGTTCC
CGCGCTCGCGCCAGGGCCTGGCGATGGGCATCTTCGGCGCCGGCAACTCGGGCGCGGCGCTGACCAAGTTCGTCGCGCCG
GCGATGATCCTGGCCGCCGGCACTTGGGAAATCGTGCCGCGCGTCTATTCGCTGGCGATGCTCGCCACCGCGCTGGTGTT
CTGGGTGTTCTCGCGCAGCAACCCCGCGCACCTGGTCAAGTCCAGCACCGGCTGGCGCGAGCAGCTTTCCATGCTGCGCG
ACCCGCGCGTGTGGCGCTACTCGCAGTACTACTCGGTGGTGTTCGGCGGCTACGTGGGCCTGTCGCTGTGGATGACCAAG
TACTACATCGGCGAATACGGTTTCGACATCAAGACCGCCGCCTTCCTGGCCGCCTGCTTCTCGCTGCCCGGCGGCGCGCT
GCGTGCCATCGGCGGCTGGATCTCCGACCGCTACGGCGCGCACCGCACCACCTGGTGGGTGATGTGGGTGAGCTGGGTCG
GCTTCTTCCTGCTGAGCTATCCGCAGACGGACTTCATCGTCCAGACCACCAGCGGTCCGCAGGGGTTCCGCATCGCGCTG
ACGCCCACGGTGTTCACCTTGCTGCTGTTCGTGGTCGGCATCGCCTTCGCCATCGGCAAGGCCTCGGTGTTCAAGTTCAT
CTCCAACGACTTCAACCACAACATCGGCGCGGTTTCCGGCGTGGTGGGCCTGGCTGGCGGGCTGGGTGGCTTTGTCCTGC
CCATTCTGTTCGGCATGCTGGCCGACCTCACCGGCATCCGCAGCAGCTGCTTCATGCTGATGTACGGCACCGTGTGCGTG
AGCCTGGTGTGGATGCACTACAGCCACCGCACCGAACGCCAGCGCAAGGAACAGGCAGTGGGCCTGACCCAGCCGGCCTG
A

Upstream 100 bases:

>100_bases
GGTTCTTCCCCGCCCCTGGCTGTGCTCCCGGCGAATGTTCAAGCGATGTGGCGGCCCCTACGCTGGCAGCACCCGTCAAA
GTTGACACAGGAGCATGGCC

Downstream 100 bases:

>100_bases
CGACCCTTCACATAACGCCACCAACCAACGCACACCATCATGTCTACCTCCGTACTCACCCGCTGGGAGCCCGAGAACGC
GGCCTTCTGGCAAAGCAAAG

Product: putative nitrite/nitrate transporter

Products: Proton [Cytoplasm]; nitrite [Periplasm] [C]

Alternate protein names: Nitrite facilitator [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MATPHPARAPIPPGAYTTLASSTFAFTICFAVWMLFAVLGIPLKKELGLNDTEFGLLAATPVLSGSLIRVPLGLWTDRYG
GRIVFFVLMLATVIPIWLISYAHSLWQLLVLGLFVGLAGGSFSVGTPYVARWFPRSRQGLAMGIFGAGNSGAALTKFVAP
AMILAAGTWEIVPRVYSLAMLATALVFWVFSRSNPAHLVKSSTGWREQLSMLRDPRVWRYSQYYSVVFGGYVGLSLWMTK
YYIGEYGFDIKTAAFLAACFSLPGGALRAIGGWISDRYGAHRTTWWVMWVSWVGFFLLSYPQTDFIVQTTSGPQGFRIAL
TPTVFTLLLFVVGIAFAIGKASVFKFISNDFNHNIGAVSGVVGLAGGLGGFVLPILFGMLADLTGIRSSCFMLMYGTVCV
SLVWMHYSHRTERQRKEQAVGLTQPA

Sequences:

>Translated_426_residues
MATPHPARAPIPPGAYTTLASSTFAFTICFAVWMLFAVLGIPLKKELGLNDTEFGLLAATPVLSGSLIRVPLGLWTDRYG
GRIVFFVLMLATVIPIWLISYAHSLWQLLVLGLFVGLAGGSFSVGTPYVARWFPRSRQGLAMGIFGAGNSGAALTKFVAP
AMILAAGTWEIVPRVYSLAMLATALVFWVFSRSNPAHLVKSSTGWREQLSMLRDPRVWRYSQYYSVVFGGYVGLSLWMTK
YYIGEYGFDIKTAAFLAACFSLPGGALRAIGGWISDRYGAHRTTWWVMWVSWVGFFLLSYPQTDFIVQTTSGPQGFRIAL
TPTVFTLLLFVVGIAFAIGKASVFKFISNDFNHNIGAVSGVVGLAGGLGGFVLPILFGMLADLTGIRSSCFMLMYGTVCV
SLVWMHYSHRTERQRKEQAVGLTQPA
>Mature_425_residues
ATPHPARAPIPPGAYTTLASSTFAFTICFAVWMLFAVLGIPLKKELGLNDTEFGLLAATPVLSGSLIRVPLGLWTDRYGG
RIVFFVLMLATVIPIWLISYAHSLWQLLVLGLFVGLAGGSFSVGTPYVARWFPRSRQGLAMGIFGAGNSGAALTKFVAPA
MILAAGTWEIVPRVYSLAMLATALVFWVFSRSNPAHLVKSSTGWREQLSMLRDPRVWRYSQYYSVVFGGYVGLSLWMTKY
YIGEYGFDIKTAAFLAACFSLPGGALRAIGGWISDRYGAHRTTWWVMWVSWVGFFLLSYPQTDFIVQTTSGPQGFRIALT
PTVFTLLLFVVGIAFAIGKASVFKFISNDFNHNIGAVSGVVGLAGGLGGFVLPILFGMLADLTGIRSSCFMLMYGTVCVS
LVWMHYSHRTERQRKEQAVGLTQPA

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787743, Length=436, Percent_Identity=27.0642201834862, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787475, Length=446, Percent_Identity=26.6816143497758, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46581; Mature: 46450

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATPHPARAPIPPGAYTTLASSTFAFTICFAVWMLFAVLGIPLKKELGLNDTEFGLLAAT
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHEEC
PVLSGSLIRVPLGLWTDRYGGRIVFFVLMLATVIPIWLISYAHSLWQLLVLGLFVGLAGG
HHHCCCEEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SFSVGTPYVARWFPRSRQGLAMGIFGAGNSGAALTKFVAPAMILAAGTWEIVPRVYSLAM
CCCCCCCHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LATALVFWVFSRSNPAHLVKSSTGWREQLSMLRDPRVWRYSQYYSVVFGGYVGLSLWMTK
HHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHH
YYIGEYGFDIKTAAFLAACFSLPGGALRAIGGWISDRYGAHRTTWWVMWVSWVGFFLLSY
HHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
PQTDFIVQTTSGPQGFRIALTPTVFTLLLFVVGIAFAIGKASVFKFISNDFNHNIGAVSG
CCCCEEEEECCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
VVGLAGGLGGFVLPILFGMLADLTGIRSSCFMLMYGTVCVSLVWMHYSHRTERQRKEQAV
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLTQPA
CCCCCC
>Mature Secondary Structure 
ATPHPARAPIPPGAYTTLASSTFAFTICFAVWMLFAVLGIPLKKELGLNDTEFGLLAAT
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHEEC
PVLSGSLIRVPLGLWTDRYGGRIVFFVLMLATVIPIWLISYAHSLWQLLVLGLFVGLAGG
HHHCCCEEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SFSVGTPYVARWFPRSRQGLAMGIFGAGNSGAALTKFVAPAMILAAGTWEIVPRVYSLAM
CCCCCCCHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LATALVFWVFSRSNPAHLVKSSTGWREQLSMLRDPRVWRYSQYYSVVFGGYVGLSLWMTK
HHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHH
YYIGEYGFDIKTAAFLAACFSLPGGALRAIGGWISDRYGAHRTTWWVMWVSWVGFFLLSY
HHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
PQTDFIVQTTSGPQGFRIALTPTVFTLLLFVVGIAFAIGKASVFKFISNDFNHNIGAVSG
CCCCEEEEECCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
VVGLAGGLGGFVLPILFGMLADLTGIRSSCFMLMYGTVCVSLVWMHYSHRTERQRKEQAV
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLTQPA
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nitrite [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: nitrite [Cytoplasm] + Proton [Periplasm] = Proton [Cytoplasm] + nitrite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9384377; 9353933 [H]