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The map label for this gene is narK2 [H]

Identifier: 38637930

GI number: 38637930

Start: 285762

End: 287156

Strand: Direct

Name: narK2 [H]

Synonym: PHG269

Alternate gene names: 38637930

Gene position: 285762-287156 (Clockwise)

Preceding gene: 38637929

Following gene: 38637931

Centisome position: 63.2

GC content: 66.81

Gene sequence:

>1395_bases
ATGTCTACCTCCGTACTCACCCGCTGGGAGCCCGAGAACGCGGCCTTCTGGCAAAGCAAAGGCGAACGCATCGCCTATCG
CAACCTCGGGATCTCGATTCCGGCGCTGATGCTGGCTTTCGTGGTCTGGATGCTGTGGAGCGTGGTCGCGGTCAACCTCG
ACCGCGCCGGCTTCCAGTTCACCAAGAACCAGCTGTTCTGGCTGACCGCGCTGCCGGCGCTGTCCGGCGCCACGCTGCGC
ATCTTCTATTCCTTCCTGGTGCCGGTGTTCGGCGGGCGCCGCTTCACCGCGATCTCCACCGCGCTGCTGCTAATCCCGGC
GCTGGGCATGGGCTTTGCGCTGCGCGACCCGTCCACCGGCTACCCGACGCTGCTGGTGCTGGCGCTGCTGTGCGGGCTGG
GCGGGGCCAACTTCAGCTCATCGATGGCCAATATCAGCTTCTTCTTCCCCAAGGCGAAGAAGGGGCTGGCCACCGGGCTC
AACGCGGGCATCGGCAACCTGGGGGTGTCGGTGGTGCAGTTCGTCACGCCGCTGGTAGTGTCGGTGGCCATCTTCGGCGC
GCTTGGCGGCGAGCCGCAGCAATTCCCGGCCAACGAGGCCAACGGCACCACGCAGAGTCTGTGGCTGCAGAACGCCGGCT
TCATCTGGGTGCCGTTCATCGTGGTATCGGCGCTGGCGGCGTGGTTCGGCATGCACGATATCGCCGATGCCAAGGCATCG
TTCGCCGAACAGGCCGTGATCTTCAAGCGCAAGCACAACTGGCTGATGTGCTGGCTGTACGTGGGCACCTTCGGTTCGTT
CATCGGCTTCTCGGCCGGGCTGGCACTGCTGACCAAGTCGCAGTTCCCGGGCGTGAACCCGACCGCCTATGCCTTCCTCG
GGCCGCTGGTGGGCGCGCTCACGCGCCCGGTGGGCGGCTGGATCTCGGACAAGCTCGGCGGCGCACGCGTCACGCTGTGG
ACCTTCGCCGGCATGATCGCCGCGGTGTTCGCGGTGCTGGCAGCGCTGCCGCATGACGGCGCCGGCGGCAACTTCATGTA
CTTCCTGGCGGCCTTCGTCGCGTTGTTCGCGCTGACCGGCATCGGCAACGGCTCGACCTTCCGCATGATCCCGGTGATCT
TCCTGACCGAGCGCCAGCGCGCTGCCGCCGGCAAGGGCGACGTGGCGCAGAAGCAGGCCCTGCAGGAAGCCGGCAAGGAA
TCCGCCGCCGTGCTGGGCTTCTCCGGTGCGATCGGCGCCTACGGCGGCTTCTTTATCCCCAAGAGCTTCGGCACCTCGCT
GGAGCTGACCGGCGCCGCCGACGCCGCGCTGTATTGCTTTATCGCCTTCTACCTCAGCTGCGTGCTGGTGACGTGGTGGC
ACTACGCGCGCCGCAACGCGCCCATGCCTTGCTAA

Upstream 100 bases:

>100_bases
CAGCCACCGCACCGAACGCCAGCGCAAGGAACAGGCAGTGGGCCTGACCCAGCCGGCCTGACGACCCTTCACATAACGCC
ACCAACCAACGCACACCATC

Downstream 100 bases:

>100_bases
CAGCCGCCGGCCGGACTAGTTCCATCGAACTAGTCCGCCCTCCGTCACCTAGCCCGACCGGCGTGCGGCGCCTGTTCCAC
CAGCGAATTGGCGCCGCCGC

Product: putative nitrite/nitrate transporter

Products: NA

Alternate protein names: Nitrite facilitator 1 [H]

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MSTSVLTRWEPENAAFWQSKGERIAYRNLGISIPALMLAFVVWMLWSVVAVNLDRAGFQFTKNQLFWLTALPALSGATLR
IFYSFLVPVFGGRRFTAISTALLLIPALGMGFALRDPSTGYPTLLVLALLCGLGGANFSSSMANISFFFPKAKKGLATGL
NAGIGNLGVSVVQFVTPLVVSVAIFGALGGEPQQFPANEANGTTQSLWLQNAGFIWVPFIVVSALAAWFGMHDIADAKAS
FAEQAVIFKRKHNWLMCWLYVGTFGSFIGFSAGLALLTKSQFPGVNPTAYAFLGPLVGALTRPVGGWISDKLGGARVTLW
TFAGMIAAVFAVLAALPHDGAGGNFMYFLAAFVALFALTGIGNGSTFRMIPVIFLTERQRAAAGKGDVAQKQALQEAGKE
SAAVLGFSGAIGAYGGFFIPKSFGTSLELTGAADAALYCFIAFYLSCVLVTWWHYARRNAPMPC

Sequences:

>Translated_464_residues
MSTSVLTRWEPENAAFWQSKGERIAYRNLGISIPALMLAFVVWMLWSVVAVNLDRAGFQFTKNQLFWLTALPALSGATLR
IFYSFLVPVFGGRRFTAISTALLLIPALGMGFALRDPSTGYPTLLVLALLCGLGGANFSSSMANISFFFPKAKKGLATGL
NAGIGNLGVSVVQFVTPLVVSVAIFGALGGEPQQFPANEANGTTQSLWLQNAGFIWVPFIVVSALAAWFGMHDIADAKAS
FAEQAVIFKRKHNWLMCWLYVGTFGSFIGFSAGLALLTKSQFPGVNPTAYAFLGPLVGALTRPVGGWISDKLGGARVTLW
TFAGMIAAVFAVLAALPHDGAGGNFMYFLAAFVALFALTGIGNGSTFRMIPVIFLTERQRAAAGKGDVAQKQALQEAGKE
SAAVLGFSGAIGAYGGFFIPKSFGTSLELTGAADAALYCFIAFYLSCVLVTWWHYARRNAPMPC
>Mature_463_residues
STSVLTRWEPENAAFWQSKGERIAYRNLGISIPALMLAFVVWMLWSVVAVNLDRAGFQFTKNQLFWLTALPALSGATLRI
FYSFLVPVFGGRRFTAISTALLLIPALGMGFALRDPSTGYPTLLVLALLCGLGGANFSSSMANISFFFPKAKKGLATGLN
AGIGNLGVSVVQFVTPLVVSVAIFGALGGEPQQFPANEANGTTQSLWLQNAGFIWVPFIVVSALAAWFGMHDIADAKASF
AEQAVIFKRKHNWLMCWLYVGTFGSFIGFSAGLALLTKSQFPGVNPTAYAFLGPLVGALTRPVGGWISDKLGGARVTLWT
FAGMIAAVFAVLAALPHDGAGGNFMYFLAAFVALFALTGIGNGSTFRMIPVIFLTERQRAAAGKGDVAQKQALQEAGKES
AAVLGFSGAIGAYGGFFIPKSFGTSLELTGAADAALYCFIAFYLSCVLVTWWHYARRNAPMPC

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=460, Percent_Identity=55.6521739130435, Blast_Score=469, Evalue=1e-133,
Organism=Escherichia coli, GI1787743, Length=461, Percent_Identity=54.2299349240781, Blast_Score=425, Evalue=1e-120,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49472; Mature: 49341

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSVLTRWEPENAAFWQSKGERIAYRNLGISIPALMLAFVVWMLWSVVAVNLDRAGFQF
CCCCCEEEECCCCCHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEE
TKNQLFWLTALPALSGATLRIFYSFLVPVFGGRRFTAISTALLLIPALGMGFALRDPSTG
CCCCEEEEEECHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEECCCCCC
YPTLLVLALLCGLGGANFSSSMANISFFFPKAKKGLATGLNAGIGNLGVSVVQFVTPLVV
CHHHHHHHHHHCCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
SVAIFGALGGEPQQFPANEANGTTQSLWLQNAGFIWVPFIVVSALAAWFGMHDIADAKAS
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FAEQAVIFKRKHNWLMCWLYVGTFGSFIGFSAGLALLTKSQFPGVNPTAYAFLGPLVGAL
HHHHHHHHEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
TRPVGGWISDKLGGARVTLWTFAGMIAAVFAVLAALPHDGAGGNFMYFLAAFVALFALTG
HHCCCCHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHC
IGNGSTFRMIPVIFLTERQRAAAGKGDVAQKQALQEAGKESAAVLGFSGAIGAYGGFFIP
CCCCCCEEEEEEEEEECHHHHCCCCCCHHHHHHHHHCCCCCEEEEECCCCHHCCCCEECC
KSFGTSLELTGAADAALYCFIAFYLSCVLVTWWHYARRNAPMPC
CCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
STSVLTRWEPENAAFWQSKGERIAYRNLGISIPALMLAFVVWMLWSVVAVNLDRAGFQF
CCCCEEEECCCCCHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEE
TKNQLFWLTALPALSGATLRIFYSFLVPVFGGRRFTAISTALLLIPALGMGFALRDPSTG
CCCCEEEEEECHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEECCCCCC
YPTLLVLALLCGLGGANFSSSMANISFFFPKAKKGLATGLNAGIGNLGVSVVQFVTPLVV
CHHHHHHHHHHCCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
SVAIFGALGGEPQQFPANEANGTTQSLWLQNAGFIWVPFIVVSALAAWFGMHDIADAKAS
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FAEQAVIFKRKHNWLMCWLYVGTFGSFIGFSAGLALLTKSQFPGVNPTAYAFLGPLVGAL
HHHHHHHHEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
TRPVGGWISDKLGGARVTLWTFAGMIAAVFAVLAALPHDGAGGNFMYFLAAFVALFALTG
HHCCCCHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHC
IGNGSTFRMIPVIFLTERQRAAAGKGDVAQKQALQEAGKESAAVLGFSGAIGAYGGFFIP
CCCCCCEEEEEEEEEECHHHHCCCCCCHHHHHHHHHCCCCCEEEEECCCCHHCCCCEECC
KSFGTSLELTGAADAALYCFIAFYLSCVLVTWWHYARRNAPMPC
CCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2668029; 8905232; 9278503; 2657652 [H]