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The map label for this gene is narX [H]

Identifier: 38637926

GI number: 38637926

Start: 279984

End: 281972

Strand: Direct

Name: narX [H]

Synonym: PHG265

Alternate gene names: 38637926

Gene position: 279984-281972 (Clockwise)

Preceding gene: 38637922

Following gene: 38637927

Centisome position: 61.92

GC content: 68.02

Gene sequence:

>1989_bases
ATGCCCGACTCCGGCGCCGATCTCGCCACGCTGCCCGCATTGCGGCACCGGCTTTCGACACGCATCATTTCGCTGTCGCT
TGCATCGCTGTTGGTCGTCCTGGCCATGATCGCCGGCACGCTGTGGCTGTCGTGGAAGCTCGAAGGCGCCGGCGCGGCGA
TCAACGATGCCGGCAGCCTGCGCATGCGCGCCAACCGCGTTGCCATCGAGCTCACACTATCGCAGGCCGGCAAGCCCAAT
GCGCTGGACGACCAGATCGCCGCGCTCGACAACACGCTCGCCCAGCTGCGCAAGGGCAACCCCGCCCGGCCACTGTTTCT
GCCGGACGAGCCGGCCATTCACGCACAGCTCGACCGCGTGGTGGACGGCTGGCAGCACCGGCTCAAGCCCTTGGCGCGGG
CCGGCGGGCTAATGGCCGGCCAGGCCACGACTGCCTACATCGACGCCCTGCCCAGCTTCGTCACGCAGGCCGACCGCTTG
GTCGGCATGATCGAGCACGTCAGCGCCAGCAAGACCGATTTGCTGCGCGTGTCGCAGGCCGGCCTGGCGGCCATGGCCTG
CGTGGGCACGGTGGCAGTGATCTACCTGCTTTACCTGTGGATCATCCTGCCGGTGCTGCGGCTGCAGGACGGTCTGAGGC
GCATGGCGGCGCGCGAGTTCTCGCTGCGCCTGCCCGTGGAGACCCGCGACGAGTTCGGCACTCTGAATGAAGGCTTCAAC
CGCATGGCCAGCGAGCTGCAGGATCTCTACCGGGACCTGGAAGCACGCGTGGCGCAGAAGACCGCCGAACTGGCGCACCA
GAACCGCGACCTGGAAACGCTGTACGAGATGGCCGCGTTCCTCAATCAGGCGACCGATGCCGAAGCCATGTGCAACGGTT
TCCTGCAGCGCGTAATGCGGCAGTTCGATGCGGACGGCGGCAGCGTGCGTGCCTTTGACGATGTCCGCGGCAAGTTGCCG
CTGATGACGTCGATCGGCTTGCCAGCGGCACTGTCCGGTGCCGCGCAATGCGTGTCGGTCAAGGCGTGCCACTGCGGCGA
CGCGACCACGCAGTCCGTGATCGCCATCCAGGACCTGCGCCATGCGCCGGCCCGCGCCGCCGGCCCGGTCAGCCCCTCGC
CCTGCACGCGCAATGGCTTCCAGGCGCTGGCCGCATTCCGCATCGGGAGCCTGCGCGGCGCAACCGGCGTCTTCGCCCTG
CATTTCCGCGAGCCGCGCGAGCTGCCGCCTTCGGACCGCCAGTTGCTGGAAACCCTCGGCCGGCACCTCGGCACCGCGCT
GGAACAGCTACGTCTGTCCGACTCCGCCCGCCAGCTGGCCGTGGTGGAGGAGCGCAACCTGGTCGCACAGGGGCTGCACG
ACAGCATCGCGCAAGGTCTGAACTTCCTCAACCTGCAGGTGCAGTTGCTTGCCGATGCTGTGGGCCGCGACGACCTTCCC
GAAGCGCGCGAGATCGTGCCGATGCTGCGCCACGGCGTGGAAGAGAGCTACCAGGACGTGCGCGAGCTGCTGAACAACTT
CCGCTCGCGGCTGACGCCGGGCGAGTTGCGTCCGGCCGTGGAAGCGACCGTGGAACGCTTCCGCCGCCAATGCCGCACCG
AGGCCACGCTGATCGTTGACGATGGCGGCGACGTCCTGCCACTGCATCCGGAGCAGCAGCTGCAGGTGCTGTTCATCCTG
CAGGAGGCGCTTTCCAATGTACGCAAACACGCGCTGGCGGCTCACGTCACCGTGCGGCTCAGCCACGGCCGCGATTTCCG
GCTGGTGGTACACGACGACGGCGAAGGCTTCGACCCTGCCGACCTCGCGCAACGCAGCGACGCCCACATCGGCCTGAACA
TCATGCGCGAACGCGCCGCGCGCCTGAACGCCCGGCTGCAGATCGACAGCCAGCCGGGGGCAGGCGTGCGCATCGCACTG
CTGTTGCCGCGCGGCGAACAACAACCCGACGCCATGGCTGCCAGCCAACTCCAAAATACCTTCCCATGA

Upstream 100 bases:

>100_bases
GCGCGCGAAGCCAGTGCTTCATCGTCTTCATTGCCACCCCGATCCTCGCCTCATGTCCAAGACCACGCCAGATCCTACTT
CCGACGAGGTGCACGAAAGC

Downstream 100 bases:

>100_bases
CCATCTGCATCCTGCTGATCGATGACCACACGCTGTTTCGCTCCGGCATCCGGGCCCTGCTGCAGCGGCAGGCCGATTTC
GAAATCGTCGACGAGGCCGC

Product: transmembrane nitrate/nitrite sensor kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 662; Mature: 661

Protein sequence:

>662_residues
MPDSGADLATLPALRHRLSTRIISLSLASLLVVLAMIAGTLWLSWKLEGAGAAINDAGSLRMRANRVAIELTLSQAGKPN
ALDDQIAALDNTLAQLRKGNPARPLFLPDEPAIHAQLDRVVDGWQHRLKPLARAGGLMAGQATTAYIDALPSFVTQADRL
VGMIEHVSASKTDLLRVSQAGLAAMACVGTVAVIYLLYLWIILPVLRLQDGLRRMAAREFSLRLPVETRDEFGTLNEGFN
RMASELQDLYRDLEARVAQKTAELAHQNRDLETLYEMAAFLNQATDAEAMCNGFLQRVMRQFDADGGSVRAFDDVRGKLP
LMTSIGLPAALSGAAQCVSVKACHCGDATTQSVIAIQDLRHAPARAAGPVSPSPCTRNGFQALAAFRIGSLRGATGVFAL
HFREPRELPPSDRQLLETLGRHLGTALEQLRLSDSARQLAVVEERNLVAQGLHDSIAQGLNFLNLQVQLLADAVGRDDLP
EAREIVPMLRHGVEESYQDVRELLNNFRSRLTPGELRPAVEATVERFRRQCRTEATLIVDDGGDVLPLHPEQQLQVLFIL
QEALSNVRKHALAAHVTVRLSHGRDFRLVVHDDGEGFDPADLAQRSDAHIGLNIMRERAARLNARLQIDSQPGAGVRIAL
LLPRGEQQPDAMAASQLQNTFP

Sequences:

>Translated_662_residues
MPDSGADLATLPALRHRLSTRIISLSLASLLVVLAMIAGTLWLSWKLEGAGAAINDAGSLRMRANRVAIELTLSQAGKPN
ALDDQIAALDNTLAQLRKGNPARPLFLPDEPAIHAQLDRVVDGWQHRLKPLARAGGLMAGQATTAYIDALPSFVTQADRL
VGMIEHVSASKTDLLRVSQAGLAAMACVGTVAVIYLLYLWIILPVLRLQDGLRRMAAREFSLRLPVETRDEFGTLNEGFN
RMASELQDLYRDLEARVAQKTAELAHQNRDLETLYEMAAFLNQATDAEAMCNGFLQRVMRQFDADGGSVRAFDDVRGKLP
LMTSIGLPAALSGAAQCVSVKACHCGDATTQSVIAIQDLRHAPARAAGPVSPSPCTRNGFQALAAFRIGSLRGATGVFAL
HFREPRELPPSDRQLLETLGRHLGTALEQLRLSDSARQLAVVEERNLVAQGLHDSIAQGLNFLNLQVQLLADAVGRDDLP
EAREIVPMLRHGVEESYQDVRELLNNFRSRLTPGELRPAVEATVERFRRQCRTEATLIVDDGGDVLPLHPEQQLQVLFIL
QEALSNVRKHALAAHVTVRLSHGRDFRLVVHDDGEGFDPADLAQRSDAHIGLNIMRERAARLNARLQIDSQPGAGVRIAL
LLPRGEQQPDAMAASQLQNTFP
>Mature_661_residues
PDSGADLATLPALRHRLSTRIISLSLASLLVVLAMIAGTLWLSWKLEGAGAAINDAGSLRMRANRVAIELTLSQAGKPNA
LDDQIAALDNTLAQLRKGNPARPLFLPDEPAIHAQLDRVVDGWQHRLKPLARAGGLMAGQATTAYIDALPSFVTQADRLV
GMIEHVSASKTDLLRVSQAGLAAMACVGTVAVIYLLYLWIILPVLRLQDGLRRMAAREFSLRLPVETRDEFGTLNEGFNR
MASELQDLYRDLEARVAQKTAELAHQNRDLETLYEMAAFLNQATDAEAMCNGFLQRVMRQFDADGGSVRAFDDVRGKLPL
MTSIGLPAALSGAAQCVSVKACHCGDATTQSVIAIQDLRHAPARAAGPVSPSPCTRNGFQALAAFRIGSLRGATGVFALH
FREPRELPPSDRQLLETLGRHLGTALEQLRLSDSARQLAVVEERNLVAQGLHDSIAQGLNFLNLQVQLLADAVGRDDLPE
AREIVPMLRHGVEESYQDVRELLNNFRSRLTPGELRPAVEATVERFRRQCRTEATLIVDDGGDVLPLHPEQQLQVLFILQ
EALSNVRKHALAAHVTVRLSHGRDFRLVVHDDGEGFDPADLAQRSDAHIGLNIMRERAARLNARLQIDSQPGAGVRIALL
LPRGEQQPDAMAASQLQNTFP

Specific function: Acts as a sensor for nitrate/nitrite and transduces signal of nitrate availability to the narL protein and of both nitrate/nitrite to the narP protein. NarX probably activates narL and narP by phosphorylation in the presence of nitrate. NarX also plays a

COG id: COG3850

COG function: function code T; Signal transduction histidine kinase, nitrate/nitrite-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=257, Percent_Identity=33.4630350194552, Blast_Score=112, Evalue=1e-25,
Organism=Escherichia coli, GI1788812, Length=243, Percent_Identity=32.9218106995885, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR016380
- InterPro:   IPR003661
- InterPro:   IPR011712
- InterPro:   IPR005467 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 72243; Mature: 72112

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDSGADLATLPALRHRLSTRIISLSLASLLVVLAMIAGTLWLSWKLEGAGAAINDAGSL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEECCCCCC
RMRANRVAIELTLSQAGKPNALDDQIAALDNTLAQLRKGNPARPLFLPDEPAIHAQLDRV
EEEECEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
VDGWQHRLKPLARAGGLMAGQATTAYIDALPSFVTQADRLVGMIEHVSASKTDLLRVSQA
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
GLAAMACVGTVAVIYLLYLWIILPVLRLQDGLRRMAAREFSLRLPVETRDEFGTLNEGFN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHCCCHHHHHH
RMASELQDLYRDLEARVAQKTAELAHQNRDLETLYEMAAFLNQATDAEAMCNGFLQRVMR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
QFDADGGSVRAFDDVRGKLPLMTSIGLPAALSGAAQCVSVKACHCGDATTQSVIAIQDLR
HHCCCCCCEEEHHHHCCCCCCHHHCCCCHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHH
HAPARAAGPVSPSPCTRNGFQALAAFRIGSLRGATGVFALHFREPRELPPSDRQLLETLG
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHH
RHLGTALEQLRLSDSARQLAVVEERNLVAQGLHDSIAQGLNFLNLQVQLLADAVGRDDLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCC
EAREIVPMLRHGVEESYQDVRELLNNFRSRLTPGELRPAVEATVERFRRQCRTEATLIVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEE
DGGDVLPLHPEQQLQVLFILQEALSNVRKHALAAHVTVRLSHGRDFRLVVHDDGEGFDPA
CCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEEEECCCCCCCHH
DLAQRSDAHIGLNIMRERAARLNARLQIDSQPGAGVRIALLLPRGEQQPDAMAASQLQNT
HHHHCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHC
FP
CC
>Mature Secondary Structure 
PDSGADLATLPALRHRLSTRIISLSLASLLVVLAMIAGTLWLSWKLEGAGAAINDAGSL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEECCCCCC
RMRANRVAIELTLSQAGKPNALDDQIAALDNTLAQLRKGNPARPLFLPDEPAIHAQLDRV
EEEECEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
VDGWQHRLKPLARAGGLMAGQATTAYIDALPSFVTQADRLVGMIEHVSASKTDLLRVSQA
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
GLAAMACVGTVAVIYLLYLWIILPVLRLQDGLRRMAAREFSLRLPVETRDEFGTLNEGFN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHCCCHHHHHH
RMASELQDLYRDLEARVAQKTAELAHQNRDLETLYEMAAFLNQATDAEAMCNGFLQRVMR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
QFDADGGSVRAFDDVRGKLPLMTSIGLPAALSGAAQCVSVKACHCGDATTQSVIAIQDLR
HHCCCCCCEEEHHHHCCCCCCHHHCCCCHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHH
HAPARAAGPVSPSPCTRNGFQALAAFRIGSLRGATGVFALHFREPRELPPSDRQLLETLG
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHH
RHLGTALEQLRLSDSARQLAVVEERNLVAQGLHDSIAQGLNFLNLQVQLLADAVGRDDLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCC
EAREIVPMLRHGVEESYQDVRELLNNFRSRLTPGELRPAVEATVERFRRQCRTEATLIVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEE
DGGDVLPLHPEQQLQVLFILQEALSNVRKHALAAHVTVRLSHGRDFRLVVHDDGEGFDPA
CCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEEEECCCCCCCHH
DLAQRSDAHIGLNIMRERAARLNARLQIDSQPGAGVRIALLLPRGEQQPDAMAASQLQNT
HHHHCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHC
FP
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]