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The map label for this gene is fabI [H]

Identifier: 38637922

GI number: 38637922

Start: 276258

End: 277046

Strand: Direct

Name: fabI [H]

Synonym: PHG261

Alternate gene names: 38637922

Gene position: 276258-277046 (Clockwise)

Preceding gene: 38637921

Following gene: 38637926

Centisome position: 61.1

GC content: 66.41

Gene sequence:

>789_bases
ATGGGCATTCTCAGTGGGAAGCGCATCCTGATCACCGGGATGCTTTCCAACCGGTCGATCGCGTTTGGCATCGCCACCGC
ATGCCGGCGCCAAGGCGCCGAACTGGCCTTCACCTATGCCGACGAACGTGTCGCGGCGCGCGTGCGCGAACTGGCGGCGG
ACTTCAGCAGCGAACTGGTCTTTCCCTGCGACGTTGCCAGCGATACGCAGATCGACGCGGCCCTGGCTTCGCTGCGCGCC
CACTGGTCGCAGCTCGATGGCCTGGTCCATGCGATCGGCTACGCGCCGCGCGAGGCGTTGTCGGGCGGCTTCCTCGACGG
GCTGTCGCGCGACAGCTTCCGCATCGCGCACGATATTTCCGCCTACAGCTTCCCCGCACTCGCCAGCGCCGCGCTGCCGA
TGCTGGCACCACGCGCCGTCTTGCTGACACTGTCTTACCTGGGCGCGCAGCGTACGCTGCCGCACTACAACGTGATGGGG
CTGGCCAAGGCCTCCCTCGAAGCCAGCGTACGGTACCTTGCCGCCGCACTGGGCGGGCGCGGCGTGCGTGTGAACGCCAT
CTCGGCGGGCCCACTGCGCACGCTGGCTGCCGCCGGCATCCGCGATTTCGGCAAGATCCTGGAACACGTGGCAAACACTG
CGGCATTGCGGCGCAATATCGACAGTGAAGAAGTTGGCAACGTGGCGGCGTTCCTGCTATCGGATCTTGCCAGCGGCATG
ACGGGGCAGGTCAGCTATGTTGACGCCGGATACCGGTGCATGGCAGCCCCGCTCGACCTGGCAGATTAG

Upstream 100 bases:

>100_bases
CCGGCGAGACGCCGGCAGCGCTGACAGTCGCACCCGCAGGCAGCAGCGGCCTGAGGCAGCCATGATATCGATCCATTCCA
CCCACTCACCACACATCCCT

Downstream 100 bases:

>100_bases
TCCCCACGCAGCGCGACCACGTCCAGTGGCGCGCTGCAATCACCTCTCGTTTGAGGCGGCAACCTGTCGCGCCAGTCCTT
CCGCATCGAGCAGGAAGATC

Product: putative enoyl-(ACP) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MGILSGKRILITGMLSNRSIAFGIATACRRQGAELAFTYADERVAARVRELAADFSSELVFPCDVASDTQIDAALASLRA
HWSQLDGLVHAIGYAPREALSGGFLDGLSRDSFRIAHDISAYSFPALASAALPMLAPRAVLLTLSYLGAQRTLPHYNVMG
LAKASLEASVRYLAAALGGRGVRVNAISAGPLRTLAAAGIRDFGKILEHVANTAALRRNIDSEEVGNVAAFLLSDLASGM
TGQVSYVDAGYRCMAAPLDLAD

Sequences:

>Translated_262_residues
MGILSGKRILITGMLSNRSIAFGIATACRRQGAELAFTYADERVAARVRELAADFSSELVFPCDVASDTQIDAALASLRA
HWSQLDGLVHAIGYAPREALSGGFLDGLSRDSFRIAHDISAYSFPALASAALPMLAPRAVLLTLSYLGAQRTLPHYNVMG
LAKASLEASVRYLAAALGGRGVRVNAISAGPLRTLAAAGIRDFGKILEHVANTAALRRNIDSEEVGNVAAFLLSDLASGM
TGQVSYVDAGYRCMAAPLDLAD
>Mature_261_residues
GILSGKRILITGMLSNRSIAFGIATACRRQGAELAFTYADERVAARVRELAADFSSELVFPCDVASDTQIDAALASLRAH
WSQLDGLVHAIGYAPREALSGGFLDGLSRDSFRIAHDISAYSFPALASAALPMLAPRAVLLTLSYLGAQRTLPHYNVMGL
AKASLEASVRYLAAALGGRGVRVNAISAGPLRTLAAAGIRDFGKILEHVANTAALRRNIDSEEVGNVAAFLLSDLASGMT
GQVSYVDAGYRCMAAPLDLAD

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=251, Percent_Identity=57.3705179282869, Blast_Score=301, Evalue=3e-83,
Organism=Escherichia coli, GI87082100, Length=259, Percent_Identity=30.5019305019305, Blast_Score=80, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24644337, Length=122, Percent_Identity=36.0655737704918, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 27570; Mature: 27439

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGILSGKRILITGMLSNRSIAFGIATACRRQGAELAFTYADERVAARVRELAADFSSELV
CCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCE
FPCDVASDTQIDAALASLRAHWSQLDGLVHAIGYAPREALSGGFLDGLSRDSFRIAHDIS
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHCCCCCCCHHHHHCC
AYSFPALASAALPMLAPRAVLLTLSYLGAQRTLPHYNVMGLAKASLEASVRYLAAALGGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
GVRVNAISAGPLRTLAAAGIRDFGKILEHVANTAALRRNIDSEEVGNVAAFLLSDLASGM
CEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
TGQVSYVDAGYRCMAAPLDLAD
CCCEEEECCCCEEEECCCCCCC
>Mature Secondary Structure 
GILSGKRILITGMLSNRSIAFGIATACRRQGAELAFTYADERVAARVRELAADFSSELV
CCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCE
FPCDVASDTQIDAALASLRAHWSQLDGLVHAIGYAPREALSGGFLDGLSRDSFRIAHDIS
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHCCCCCCCHHHHHCC
AYSFPALASAALPMLAPRAVLLTLSYLGAQRTLPHYNVMGLAKASLEASVRYLAAALGGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
GVRVNAISAGPLRTLAAAGIRDFGKILEHVANTAALRRNIDSEEVGNVAAFLLSDLASGM
CEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
TGQVSYVDAGYRCMAAPLDLAD
CCCEEEECCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]