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The map label for this gene is ebgR [H]

Identifier: 37680824

GI number: 37680824

Start: 2670852

End: 2671868

Strand: Direct

Name: ebgR [H]

Synonym: VV2640

Alternate gene names: 37680824

Gene position: 2670852-2671868 (Clockwise)

Preceding gene: 37680818

Following gene: 37680825

Centisome position: 79.62

GC content: 49.75

Gene sequence:

>1017_bases
GTGCCAACAGGAAGAAGAATGGCTACGTTAAAAGATATTGCCACGGAAGCGGGCGTTTCCCTGGCCACGGTGTCGCGAGT
GCTCAATGAAGACCCGACTCTGAGTGTCAAAGAAGAGACCAAACACCGCATCTTAGAAATTGCGGAGAAATTGGAATACC
GCACCAGCAGCGCGAAAAAGAACAGTGTTGCCGCCGTACCGGCCCATCATTTCCTGGCATTGTACAACTACAAGCCAGAA
GCTGAGATTAATGATCCTTACTATCTCTCCATTCGCCACGGCATTGAAACCCAGTGTGAAAAACTCGGCATCAATTTAAC
CAATTGCTACGACAGCAAAATCGACCCCGACGTGAAAAAGATCACCGGTGTCCTTATGGTTGGCAAAACCACGCCTGCCG
CGATGTTGCGCGCCAGCAAGATGACCGACAACATCTGCTATCTCGATTTTAGCGATGACCATGGCGACTTCGACAGCGTG
GATATTGATCTGGTAAAAATCAGTAAAGAAGTAACGGATTTCTTTATCAGCCAAGGCTTTGAGCGCATCGGTTTTATTGG
TGGTCAAGACGAACCCGGCCAAGCCGACATTCGCGAAATGGCCTTTGCTAAATATGGTCAGTTGAAAGGCGTGGTCAACG
AGTCAGACATTTATCGCGGTGATTTTTCTAGCGCTTCTGGTTACAAACTGGCGCAAGCGATGTTGGAAAAGGGCGACTAT
CCGAAGGCGCTGTTTATCGCTTCCGATTCCATCGCGATTGGCGTGCTGCGTGCTTTGCTCGAACATGGGCTCAACATTCC
CCGCGATATTTCGCTGATCAGCGTCAACGACATTCCCAACGCCAGATTCACTTTCCCGCCTCTCTCTACGGTGAGAATTC
ACTCAGAAATGATGGGCATTCAAGGCGTTAACTTATTGGTGGAAAAAGCGCGTGATGGCCGAGCACTGCCGCTTAAAGTG
TATGTGCCAAGCAAGCTCAAGCTAAGAGGAACGACTCAGGAGAGGAGCCAAGCATGA

Upstream 100 bases:

>100_bases
AAAAACACTCACGGCTCGATTTTTACTAAAAAAAACGGATGCATCACGCAGCCTCTTGATTACAATAGAGAACAGATGAA
TTCACTCAGCACAGTGCGCT

Downstream 100 bases:

>100_bases
ACCACGAGCAACTTCTTAAGGAGCTGGAAGAAAACGCCTGTCTTGATCTGCATAAACTCTATTACGCCAACCCAGATAAG
CGCTTTTACGCTCTGCTGGC

Product: DNA-binding transcriptional repressor EbgR

Products: NA

Alternate protein names: Ebg operon repressor [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MPTGRRMATLKDIATEAGVSLATVSRVLNEDPTLSVKEETKHRILEIAEKLEYRTSSAKKNSVAAVPAHHFLALYNYKPE
AEINDPYYLSIRHGIETQCEKLGINLTNCYDSKIDPDVKKITGVLMVGKTTPAAMLRASKMTDNICYLDFSDDHGDFDSV
DIDLVKISKEVTDFFISQGFERIGFIGGQDEPGQADIREMAFAKYGQLKGVVNESDIYRGDFSSASGYKLAQAMLEKGDY
PKALFIASDSIAIGVLRALLEHGLNIPRDISLISVNDIPNARFTFPPLSTVRIHSEMMGIQGVNLLVEKARDGRALPLKV
YVPSKLKLRGTTQERSQA

Sequences:

>Translated_338_residues
MPTGRRMATLKDIATEAGVSLATVSRVLNEDPTLSVKEETKHRILEIAEKLEYRTSSAKKNSVAAVPAHHFLALYNYKPE
AEINDPYYLSIRHGIETQCEKLGINLTNCYDSKIDPDVKKITGVLMVGKTTPAAMLRASKMTDNICYLDFSDDHGDFDSV
DIDLVKISKEVTDFFISQGFERIGFIGGQDEPGQADIREMAFAKYGQLKGVVNESDIYRGDFSSASGYKLAQAMLEKGDY
PKALFIASDSIAIGVLRALLEHGLNIPRDISLISVNDIPNARFTFPPLSTVRIHSEMMGIQGVNLLVEKARDGRALPLKV
YVPSKLKLRGTTQERSQA
>Mature_337_residues
PTGRRMATLKDIATEAGVSLATVSRVLNEDPTLSVKEETKHRILEIAEKLEYRTSSAKKNSVAAVPAHHFLALYNYKPEA
EINDPYYLSIRHGIETQCEKLGINLTNCYDSKIDPDVKKITGVLMVGKTTPAAMLRASKMTDNICYLDFSDDHGDFDSVD
IDLVKISKEVTDFFISQGFERIGFIGGQDEPGQADIREMAFAKYGQLKGVVNESDIYRGDFSSASGYKLAQAMLEKGDYP
KALFIASDSIAIGVLRALLEHGLNIPRDISLISVNDIPNARFTFPPLSTVRIHSEMMGIQGVNLLVEKARDGRALPLKVY
VPSKLKLRGTTQERSQA

Specific function: Repressor for beta galactosidase alpha and beta subunits (ebgA and ebgC). Binds lactose as an inducer [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789456, Length=327, Percent_Identity=74.6177370030581, Blast_Score=509, Evalue=1e-146,
Organism=Escherichia coli, GI1787948, Length=333, Percent_Identity=28.5285285285285, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1790369, Length=339, Percent_Identity=27.4336283185841, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1790194, Length=316, Percent_Identity=26.8987341772152, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1786540, Length=344, Percent_Identity=24.7093023255814, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1788474, Length=324, Percent_Identity=25.6172839506173, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1789202, Length=353, Percent_Identity=24.929178470255, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1789068, Length=309, Percent_Identity=27.831715210356, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787580, Length=342, Percent_Identity=23.6842105263158, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37364; Mature: 37233

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTGRRMATLKDIATEAGVSLATVSRVLNEDPTLSVKEETKHRILEIAEKLEYRTSSAKK
CCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCC
NSVAAVPAHHFLALYNYKPEAEINDPYYLSIRHGIETQCEKLGINLTNCYDSKIDPDVKK
CCEEEECHHHEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHCCEEEECCCCCCCCCHHH
ITGVLMVGKTTPAAMLRASKMTDNICYLDFSDDHGDFDSVDIDLVKISKEVTDFFISQGF
HEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHCCH
ERIGFIGGQDEPGQADIREMAFAKYGQLKGVVNESDIYRGDFSSASGYKLAQAMLEKGDY
HHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCC
PKALFIASDSIAIGVLRALLEHGLNIPRDISLISVNDIPNARFTFPPLSTVRIHSEMMGI
CCEEEEECCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEECCCCCCEEHHHHHHCC
QGVNLLVEKARDGRALPLKVYVPSKLKLRGTTQERSQA
CCHHHHHHHCCCCCEEEEEEECCCCEEEECCCHHHCCC
>Mature Secondary Structure 
PTGRRMATLKDIATEAGVSLATVSRVLNEDPTLSVKEETKHRILEIAEKLEYRTSSAKK
CCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCC
NSVAAVPAHHFLALYNYKPEAEINDPYYLSIRHGIETQCEKLGINLTNCYDSKIDPDVKK
CCEEEECHHHEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHCCEEEECCCCCCCCCHHH
ITGVLMVGKTTPAAMLRASKMTDNICYLDFSDDHGDFDSVDIDLVKISKEVTDFFISQGF
HEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHCCH
ERIGFIGGQDEPGQADIREMAFAKYGQLKGVVNESDIYRGDFSSASGYKLAQAMLEKGDY
HHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCC
PKALFIASDSIAIGVLRALLEHGLNIPRDISLISVNDIPNARFTFPPLSTVRIHSEMMGI
CCEEEEECCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEECCCCCCEEHHHHHHCC
QGVNLLVEKARDGRALPLKVYVPSKLKLRGTTQERSQA
CCHHHHHHHCCCCCEEEEEEECCCCEEEECCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2515108; 3939708; 9278503 [H]