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The map label for this gene is glpK

Identifier: 37680808

GI number: 37680808

Start: 2652801

End: 2654318

Strand: Direct

Name: glpK

Synonym: VV2624

Alternate gene names: 37680808

Gene position: 2652801-2654318 (Clockwise)

Preceding gene: 37680807

Following gene: 37680809

Centisome position: 79.08

GC content: 52.37

Gene sequence:

>1518_bases
ATGACTGAGCAGAAGTACATCGTTGCACTGGACCAAGGCACCACCAGCTCTCGTGCTGTGGTTTTAGATCACGACGCGAA
CATCGTTTCTGTCTCCCAGCGCGAATTCACTCAGATTTACCCACAAGCAGGCTGGGTAGAACATGATCCAATGGAAATTT
ACGCCACTCAGAGCTCTACACTCGTTGAAGCATTGGGTAAAAAGGGCATCCGCAGTGACCAAGTGGCTGCCATCGGTATC
ACCAACCAACGTGAAACCACCATCGTTTGGAACAAAGAAACAGGCAAACCGGTTTACAACGCCATTGTTTGGCAGTGCCG
TCGTACCGCCGATATCTGTGAGGACCTAAAAGCGCGCGGATTGGAATCTTACATTCGCGAAAACACCGGCCTTGTACTTG
ACCCTTACTTCTCGGGCACCAAAGTAAAGTGGATTCTCGATAATGTTGAAGGGGCACGTGAAGACGCCGAAGCGGGTAAA
CTGCTGTTTGGTACCGTTGATACTTGGCTGGTCTGGAAGATGACCCAAGGCCGCGTGCACGTCACCGACTACACCAACGC
CTCCCGCACCATGCTGTTCAACATCAATGACCTATGTTGGGATAGCAAACTGCTCGAAGAAATGGGCATCCCAGCGTCGA
TGATGCCGGAAGTAAAACGCTCATCGGAAATTTACGGCCAAACCAACATCGGTGGTAAAGGCGGTACGCGTATTCCTATC
TCAGGCATTGCGGGTGACCAACAAGCGGCGCTGTACGGCCAAATGTGTGTCGAAGCTGGCCAAGCGAAAAATACCTACGG
AACTGGCTGTTTCTTGTTGATGAACACCGGACAAGAGAAAGTGACCTCACGCAACGGTTTGTTGACCACACTCGCGTGTG
GACCAAAAGGTGAGCCAGCCTACGCACTGGAAGGTGCGGTATTTATGGGCGGCGCTTCGATTCAGTGGCTGCGTGATGAG
CTGAAACTGATTTCCGATGCGCACGATTCCGAATACTTCGCCACAAAAGTGGACACCTCAAACGGCGTGTATGTGGTGCC
AGCCTTTACGGGCTTAGGCGCTCCGTACTGGGATGCCTATGCTCGCGGTACCATTGTTGGCCTCACTCGTGGCGTTAACT
CAAACCACATCATCCGTGCAACGTTAGAAGGCATTGCCTACCAAACCCGTGATGTACTGGACGCAATGCAAGCAGACTCA
GGCATTAAGCTCTCTGCCCTACGCGTTGATGGCGGCGCTGTCGCCAACAACTTCCTCATGCAGTTCCAATCTGATGTGCT
TGATACCGAAGTGCATCGTCCAAAAGTCACCGAAGTGACCGCGCTGGGCGCTGCGTACCTAGCAGGCTTGGCCGTGGGGT
ACTGGAACAGCATTGATGAACTTCAAGGCAAAGCAGAGATCGACCGCAGCTTCCTTCCTCATCAAGATGAAGAGAAACGC
AGCCGACGCTATAAAGGCTGGAAACGCGCAGTCAAATGTGCGCAAACTTGGTCTGAATTGCGCGATCTCGAAGATTAA

Upstream 100 bases:

>100_bases
ACGGAAGAAGAAGCCAAAGAAGCACGCGCATAATTAGCCGCTTGCACTGAACGACATAAAATTAACTAAAATAGTAATAA
CGAAACAAAAGGATCCTACC

Downstream 100 bases:

>100_bases
AATCGCCTCCCCCCAATTTTGGGCGTCTTTTGACGCCCTTTTTTGTTGTTTCACACGAATTCTTGACCTCGACAACTTCT
ATGAACTCATCATTTAGCGC

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MTEQKYIVALDQGTTSSRAVVLDHDANIVSVSQREFTQIYPQAGWVEHDPMEIYATQSSTLVEALGKKGIRSDQVAAIGI
TNQRETTIVWNKETGKPVYNAIVWQCRRTADICEDLKARGLESYIRENTGLVLDPYFSGTKVKWILDNVEGAREDAEAGK
LLFGTVDTWLVWKMTQGRVHVTDYTNASRTMLFNINDLCWDSKLLEEMGIPASMMPEVKRSSEIYGQTNIGGKGGTRIPI
SGIAGDQQAALYGQMCVEAGQAKNTYGTGCFLLMNTGQEKVTSRNGLLTTLACGPKGEPAYALEGAVFMGGASIQWLRDE
LKLISDAHDSEYFATKVDTSNGVYVVPAFTGLGAPYWDAYARGTIVGLTRGVNSNHIIRATLEGIAYQTRDVLDAMQADS
GIKLSALRVDGGAVANNFLMQFQSDVLDTEVHRPKVTEVTALGAAYLAGLAVGYWNSIDELQGKAEIDRSFLPHQDEEKR
SRRYKGWKRAVKCAQTWSELRDLED

Sequences:

>Translated_505_residues
MTEQKYIVALDQGTTSSRAVVLDHDANIVSVSQREFTQIYPQAGWVEHDPMEIYATQSSTLVEALGKKGIRSDQVAAIGI
TNQRETTIVWNKETGKPVYNAIVWQCRRTADICEDLKARGLESYIRENTGLVLDPYFSGTKVKWILDNVEGAREDAEAGK
LLFGTVDTWLVWKMTQGRVHVTDYTNASRTMLFNINDLCWDSKLLEEMGIPASMMPEVKRSSEIYGQTNIGGKGGTRIPI
SGIAGDQQAALYGQMCVEAGQAKNTYGTGCFLLMNTGQEKVTSRNGLLTTLACGPKGEPAYALEGAVFMGGASIQWLRDE
LKLISDAHDSEYFATKVDTSNGVYVVPAFTGLGAPYWDAYARGTIVGLTRGVNSNHIIRATLEGIAYQTRDVLDAMQADS
GIKLSALRVDGGAVANNFLMQFQSDVLDTEVHRPKVTEVTALGAAYLAGLAVGYWNSIDELQGKAEIDRSFLPHQDEEKR
SRRYKGWKRAVKCAQTWSELRDLED
>Mature_504_residues
TEQKYIVALDQGTTSSRAVVLDHDANIVSVSQREFTQIYPQAGWVEHDPMEIYATQSSTLVEALGKKGIRSDQVAAIGIT
NQRETTIVWNKETGKPVYNAIVWQCRRTADICEDLKARGLESYIRENTGLVLDPYFSGTKVKWILDNVEGAREDAEAGKL
LFGTVDTWLVWKMTQGRVHVTDYTNASRTMLFNINDLCWDSKLLEEMGIPASMMPEVKRSSEIYGQTNIGGKGGTRIPIS
GIAGDQQAALYGQMCVEAGQAKNTYGTGCFLLMNTGQEKVTSRNGLLTTLACGPKGEPAYALEGAVFMGGASIQWLRDEL
KLISDAHDSEYFATKVDTSNGVYVVPAFTGLGAPYWDAYARGTIVGLTRGVNSNHIIRATLEGIAYQTRDVLDAMQADSG
IKLSALRVDGGAVANNFLMQFQSDVLDTEVHRPKVTEVTALGAAYLAGLAVGYWNSIDELQGKAEIDRSFLPHQDEEKRS
RRYKGWKRAVKCAQTWSELRDLED

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI189409120, Length=502, Percent_Identity=47.609561752988, Blast_Score=437, Evalue=1e-122,
Organism=Homo sapiens, GI4504007, Length=502, Percent_Identity=47.609561752988, Blast_Score=437, Evalue=1e-122,
Organism=Homo sapiens, GI42794763, Length=506, Percent_Identity=47.4308300395257, Blast_Score=434, Evalue=1e-122,
Organism=Homo sapiens, GI41393575, Length=502, Percent_Identity=45.8167330677291, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI88196792, Length=509, Percent_Identity=32.6129666011788, Blast_Score=304, Evalue=2e-82,
Organism=Escherichia coli, GI1790361, Length=499, Percent_Identity=79.7595190380761, Blast_Score=838, Evalue=0.0,
Organism=Escherichia coli, GI1789987, Length=481, Percent_Identity=24.7401247401247, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1787789, Length=485, Percent_Identity=25.360824742268, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1789168, Length=509, Percent_Identity=22.2003929273084, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1789138, Length=492, Percent_Identity=21.1382113821138, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17535599, Length=504, Percent_Identity=42.2619047619048, Blast_Score=410, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI71986140, Length=514, Percent_Identity=25.0972762645914, Blast_Score=168, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI71986147, Length=260, Percent_Identity=31.1538461538462, Blast_Score=120, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6321755, Length=561, Percent_Identity=37.4331550802139, Blast_Score=317, Evalue=3e-87,
Organism=Drosophila melanogaster, GI17864214, Length=507, Percent_Identity=44.7731755424063, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24655555, Length=502, Percent_Identity=42.0318725099602, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24655560, Length=502, Percent_Identity=42.0318725099602, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24655570, Length=502, Percent_Identity=42.0318725099602, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24655574, Length=502, Percent_Identity=42.0318725099602, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24655565, Length=502, Percent_Identity=42.0318725099602, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24654573, Length=440, Percent_Identity=46.5909090909091, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI19922058, Length=521, Percent_Identity=33.7811900191939, Blast_Score=291, Evalue=6e-79,
Organism=Drosophila melanogaster, GI24652871, Length=500, Percent_Identity=33.8, Blast_Score=276, Evalue=2e-74,
Organism=Drosophila melanogaster, GI24656367, Length=514, Percent_Identity=26.4591439688716, Blast_Score=196, Evalue=3e-50,
Organism=Drosophila melanogaster, GI21357867, Length=526, Percent_Identity=26.0456273764259, Blast_Score=194, Evalue=1e-49,
Organism=Drosophila melanogaster, GI24656372, Length=349, Percent_Identity=28.6532951289398, Blast_Score=159, Evalue=5e-39,
Organism=Drosophila melanogaster, GI45552909, Length=289, Percent_Identity=27.681660899654, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 55633; Mature: 55502

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00933 FGGY_KINASES_1 ; PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQKYIVALDQGTTSSRAVVLDHDANIVSVSQREFTQIYPQAGWVEHDPMEIYATQSST
CCCCEEEEEEECCCCCCCEEEEECCCCEEEECHHHHHHHCCCCCCCCCCCEEEEECCCHH
LVEALGKKGIRSDQVAAIGITNQRETTIVWNKETGKPVYNAIVWQCRRTADICEDLKARG
HHHHHHHCCCCCCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCC
LESYIRENTGLVLDPYFSGTKVKWILDNVEGAREDAEAGKLLFGTVDTWLVWKMTQGRVH
HHHHHHCCCCEEEECCCCCCEEEEEEECCCCCCCHHHCCCEEEEECCEEEEEEEECCEEE
VTDYTNASRTMLFNINDLCWDSKLLEEMGIPASMMPEVKRSSEIYGQTNIGGKGGTRIPI
EEECCCCCCEEEEECHHHHCCHHHHHHHCCCHHHHHHHHHCHHHCCCCCCCCCCCCEEEE
SGIAGDQQAALYGQMCVEAGQAKNTYGTGCFLLMNTGQEKVTSRNGLLTTLACGPKGEPA
CCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCHHHHHCCCCCEEEEECCCCCCCC
YALEGAVFMGGASIQWLRDELKLISDAHDSEYFATKVDTSNGVYVVPAFTGLGAPYWDAY
EEECCEEEECCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEECCCCCCCHHHHH
ARGTIVGLTRGVNSNHIIRATLEGIAYQTRDVLDAMQADSGIKLSALRVDGGAVANNFLM
HCCEEEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHH
QFQSDVLDTEVHRPKVTEVTALGAAYLAGLAVGYWNSIDELQGKAEIDRSFLPHQDEEKR
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHCCCCCCCHHHH
SRRYKGWKRAVKCAQTWSELRDLED
HHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TEQKYIVALDQGTTSSRAVVLDHDANIVSVSQREFTQIYPQAGWVEHDPMEIYATQSST
CCCEEEEEEECCCCCCCEEEEECCCCEEEECHHHHHHHCCCCCCCCCCCEEEEECCCHH
LVEALGKKGIRSDQVAAIGITNQRETTIVWNKETGKPVYNAIVWQCRRTADICEDLKARG
HHHHHHHCCCCCCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCC
LESYIRENTGLVLDPYFSGTKVKWILDNVEGAREDAEAGKLLFGTVDTWLVWKMTQGRVH
HHHHHHCCCCEEEECCCCCCEEEEEEECCCCCCCHHHCCCEEEEECCEEEEEEEECCEEE
VTDYTNASRTMLFNINDLCWDSKLLEEMGIPASMMPEVKRSSEIYGQTNIGGKGGTRIPI
EEECCCCCCEEEEECHHHHCCHHHHHHHCCCHHHHHHHHHCHHHCCCCCCCCCCCCEEEE
SGIAGDQQAALYGQMCVEAGQAKNTYGTGCFLLMNTGQEKVTSRNGLLTTLACGPKGEPA
CCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCHHHHHCCCCCEEEEECCCCCCCC
YALEGAVFMGGASIQWLRDELKLISDAHDSEYFATKVDTSNGVYVVPAFTGLGAPYWDAY
EEECCEEEECCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEECCCCCCCHHHHH
ARGTIVGLTRGVNSNHIIRATLEGIAYQTRDVLDAMQADSGIKLSALRVDGGAVANNFLM
HCCEEEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHH
QFQSDVLDTEVHRPKVTEVTALGAAYLAGLAVGYWNSIDELQGKAEIDRSFLPHQDEEKR
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHCCCCCCCHHHH
SRRYKGWKRAVKCAQTWSELRDLED
HHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA