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The map label for this gene is glpF [H]

Identifier: 37680807

GI number: 37680807

Start: 2651882

End: 2652733

Strand: Direct

Name: glpF [H]

Synonym: VV2623

Alternate gene names: 37680807

Gene position: 2651882-2652733 (Clockwise)

Preceding gene: 37680806

Following gene: 37680808

Centisome position: 79.05

GC content: 51.29

Gene sequence:

>852_bases
ATGACTAACAAAAAACACACTCTTTTCGGAGAGTGCCTGGCCGAATTCATCGGCACAGGGCTACTTATCTTTTTCGGTGT
TGGCTGCGTGGCAGCACTGGTGCTTGCTGGTGCTCAGTTCGGCCAATGGGAAATCAGTATCGTTTGGGGCTTTGGTGTCT
CGATCGCGATTTACTGTACGGCTGGGGTATCCGGCGCACACATCAACCCCGCGGTCACCATTGCCTTAGCGGCATTCCAC
GGCTTCGATAAAGCGAAAGTGGTCCCTTACATCATCGCGCAGTTACTGGGCGCATTCTGCTCTGCGGCTCTGGTTTATAC
CCTCTACAGCAACTTGTTTACTGACTACGAGATCGCGCATAACTTTGTTCGCAGCAGCCAAGAAGCTCTTGCTACAGCAG
GCATCTTCTCCACTTACCCACACGCTTCACTCTCCTTCTTTGGCGCGTTTGCGGTGGAATTTGTCATTACTGCAGTGTTG
ATGTTTGCCATCCTCGCCTTAGGTGACGAACATAATGGTGCACCGCGTGGAGCCATGAACCCACTGTTGATCGGTATCTT
GATTGCGGTGATCGGTGGATCATTAGGTCCACTAACGGGCTTCGCCATGAACCCAGCACGTGACTTCGGACCAAAACTCT
TTGCTTATTTTGCTGGTTGGGATTATGCCCTAAGCGGTGGCCGAGACATTCCTTACTTCATCGTACCGATTTTGGCACCA
ATTGCCGGTGCGTGTTTTGGTGGTTGGCTCTATCCAAAAGCGATTGCGGCATACTTACCGAAAGTAGGCTCAGGTTGCAC
CATTCCGAACCAATGTGAAACGGAAGAAGAAGCCAAAGAAGCACGCGCATAA

Upstream 100 bases:

>100_bases
GTTTGTTTATTTTCGCAATCGTCTAAATGTGCAAAATAGCTTGTGTGCATGCAGTAGATAATAAAAAGCACAAATAACGT
GAACTTAAAGGACGCTTACC

Downstream 100 bases:

>100_bases
TTAGCCGCTTGCACTGAACGACATAAAATTAACTAAAATAGTAATAACGAAACAAAAGGATCCTACCATGACTGAGCAGA
AGTACATCGTTGCACTGGAC

Product: glycerol uptake facilitator

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MTNKKHTLFGECLAEFIGTGLLIFFGVGCVAALVLAGAQFGQWEISIVWGFGVSIAIYCTAGVSGAHINPAVTIALAAFH
GFDKAKVVPYIIAQLLGAFCSAALVYTLYSNLFTDYEIAHNFVRSSQEALATAGIFSTYPHASLSFFGAFAVEFVITAVL
MFAILALGDEHNGAPRGAMNPLLIGILIAVIGGSLGPLTGFAMNPARDFGPKLFAYFAGWDYALSGGRDIPYFIVPILAP
IAGACFGGWLYPKAIAAYLPKVGSGCTIPNQCETEEEAKEARA

Sequences:

>Translated_283_residues
MTNKKHTLFGECLAEFIGTGLLIFFGVGCVAALVLAGAQFGQWEISIVWGFGVSIAIYCTAGVSGAHINPAVTIALAAFH
GFDKAKVVPYIIAQLLGAFCSAALVYTLYSNLFTDYEIAHNFVRSSQEALATAGIFSTYPHASLSFFGAFAVEFVITAVL
MFAILALGDEHNGAPRGAMNPLLIGILIAVIGGSLGPLTGFAMNPARDFGPKLFAYFAGWDYALSGGRDIPYFIVPILAP
IAGACFGGWLYPKAIAAYLPKVGSGCTIPNQCETEEEAKEARA
>Mature_282_residues
TNKKHTLFGECLAEFIGTGLLIFFGVGCVAALVLAGAQFGQWEISIVWGFGVSIAIYCTAGVSGAHINPAVTIALAAFHG
FDKAKVVPYIIAQLLGAFCSAALVYTLYSNLFTDYEIAHNFVRSSQEALATAGIFSTYPHASLSFFGAFAVEFVITAVLM
FAILALGDEHNGAPRGAMNPLLIGILIAVIGGSLGPLTGFAMNPARDFGPKLFAYFAGWDYALSGGRDIPYFIVPILAPI
AGACFGGWLYPKAIAAYLPKVGSGCTIPNQCETEEEAKEARA

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI157266307, Length=279, Percent_Identity=39.426523297491, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI4826645, Length=285, Percent_Identity=36.8421052631579, Blast_Score=154, Evalue=8e-38,
Organism=Homo sapiens, GI22538420, Length=259, Percent_Identity=35.9073359073359, Blast_Score=148, Evalue=5e-36,
Organism=Homo sapiens, GI4502187, Length=258, Percent_Identity=34.1085271317829, Blast_Score=145, Evalue=4e-35,
Organism=Homo sapiens, GI310133356, Length=205, Percent_Identity=30.7317073170732, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI310114181, Length=205, Percent_Identity=30.7317073170732, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI45446752, Length=211, Percent_Identity=28.436018957346, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI4502179, Length=272, Percent_Identity=29.7794117647059, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI86792455, Length=204, Percent_Identity=34.3137254901961, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI4502181, Length=209, Percent_Identity=32.0574162679426, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI4755125, Length=209, Percent_Identity=32.0574162679426, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1790362, Length=276, Percent_Identity=63.4057971014493, Blast_Score=342, Evalue=2e-95,
Organism=Escherichia coli, GI1787101, Length=216, Percent_Identity=30.5555555555556, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71994009, Length=281, Percent_Identity=34.1637010676157, Blast_Score=147, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI32564052, Length=272, Percent_Identity=32.7205882352941, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17533613, Length=272, Percent_Identity=32.7205882352941, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17544068, Length=240, Percent_Identity=35.8333333333333, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17531429, Length=260, Percent_Identity=35.7692307692308, Blast_Score=131, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17531431, Length=260, Percent_Identity=35.7692307692308, Blast_Score=131, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI71992966, Length=243, Percent_Identity=34.5679012345679, Blast_Score=129, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71992961, Length=243, Percent_Identity=31.2757201646091, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI71993722, Length=244, Percent_Identity=27.0491803278689, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17558372, Length=221, Percent_Identity=30.7692307692308, Blast_Score=68, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6321054, Length=211, Percent_Identity=39.3364928909953, Blast_Score=142, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6322985, Length=290, Percent_Identity=29.3103448275862, Blast_Score=108, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24762344, Length=216, Percent_Identity=34.2592592592593, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24762346, Length=216, Percent_Identity=34.2592592592593, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24762348, Length=216, Percent_Identity=34.2592592592593, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI20130305, Length=216, Percent_Identity=34.2592592592593, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI45550503, Length=210, Percent_Identity=31.4285714285714, Blast_Score=91, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 29828; Mature: 29697

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNKKHTLFGECLAEFIGTGLLIFFGVGCVAALVLAGAQFGQWEISIVWGFGVSIAIYCT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEEEE
AGVSGAHINPAVTIALAAFHGFDKAKVVPYIIAQLLGAFCSAALVYTLYSNLFTDYEIAH
CCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NFVRSSQEALATAGIFSTYPHASLSFFGAFAVEFVITAVLMFAILALGDEHNGAPRGAMN
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
PLLIGILIAVIGGSLGPLTGFAMNPARDFGPKLFAYFAGWDYALSGGRDIPYFIVPILAP
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHCHHHHHHHHCCHHHCCCCCCCHHHHHHHHHH
IAGACFGGWLYPKAIAAYLPKVGSGCTIPNQCETEEEAKEARA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCC
>Mature Secondary Structure 
TNKKHTLFGECLAEFIGTGLLIFFGVGCVAALVLAGAQFGQWEISIVWGFGVSIAIYCT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEEEE
AGVSGAHINPAVTIALAAFHGFDKAKVVPYIIAQLLGAFCSAALVYTLYSNLFTDYEIAH
CCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NFVRSSQEALATAGIFSTYPHASLSFFGAFAVEFVITAVLMFAILALGDEHNGAPRGAMN
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
PLLIGILIAVIGGSLGPLTGFAMNPARDFGPKLFAYFAGWDYALSGGRDIPYFIVPILAP
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHCHHHHHHHHCCHHHCCCCCCCHHHHHHHHHH
IAGACFGGWLYPKAIAAYLPKVGSGCTIPNQCETEEEAKEARA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]